Ube2d2b (NM_001276397) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700013N18Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225915 representing NM_001276397 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTGAAGAGAATCCACAAGGAACTGAACGACCTGGCCCAGGATCCCCCAGCACAGTGTTCAGCAG GTCCTGTCGGGGAAGATATGTTTCACTGGCAAGCTACAATCATGGGGCCAAATGATAGTCCCTATCAGGG CGGAGCATTTTTCTTGACAATTGATTTCCCAACAGAGTACCCCTTCAAACCACCTAAGGTTGAATTTACA ACAAGAATTTATCATCCAAATGTTAACAGTAACGGCAGTATTTGTCTTGATATTCTTCGGTCACAGTGGT CTCCAGCACTAACTATTTCCAAAGTACTTTTGTCCATCAGTTCTCTGTTGTGTGACCCCAATCCAGATGA TCCCTTAGTGCCTGAGATTGCTCAGATCTACAAAACAGATAGAGACAAGTACAACAGAACAGCTCGGGAA TGGACTCAGAAATATGCGATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276397
Insert Size	444 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276397.1, NP_001263326.1
RefSeq Size	1616 bp
RefSeq ORF	444 bp
Locus ID	73318
UniProt ID	Q6ZWY6
Gene Summary	Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection (By similarity). Plays a role in early maturation of the testis.[UniProtKB/Swiss-Prot Function]

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