Dchs1 (NM_001162943) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110041P15Rik; C130033F22Rik; CDH25; FIB1; Gm165; PCDH16
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225474 representing NM_001162943 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAAGGAGCTGAGTGTCGCACTTTCCTGCCCTGGCATGAAGAGCCTCAGGACTCTATTACCATTGC TGGTGCTGTTAGGGGCTACAGTGCCAGGAAGCTGGGGTCAGGCTGGGAGCCTGGACCTACAGATCGACGA GGAACAACCAGCAGGCACGCTGATTGGAGACATCAGTGCAGGGCTTCCACCAGGCACAGCGCCTCCTCCC ATGTACTTCATCTCTGCCCAGGAAGGCAGTGGCGTAGGCACAGACCTTGCTATTGATGAGCACAGTGGAG TGGTCCGTACAGCTCGGGTCCTAGACCGTGAGCGGAGAGACCGTTACCGCTTCACTGCAGTCACTCCTGA TGGGGCCACCGTGGAAGTTACAGTGAGAGTAGCTGATATCAATGACCACGCACCAGCTTTCCCTCAGGCT CGGGCAGCCCTGCAGATACCTGAGCATACAGCTCTTGGTACACGTTACCCACTGGAGCCTGCTCGTGATG CAGATGCCGGCCGCCTAGGAACCCAAGGGTATGCGCTATCTGGTGATGGGGCTGGAGAGACCTTTCGGCT GGAGACACGTCCTGGTCCTGGTGGAGCTCCAGTACCTGAGCTGGTGATTGCCGGAGAACTGGACCGGGAG AACCGCTCACACTACATGTTGCAGCTCGAAGCCTACGATGGTGGCTCACCTCCGCGGAGAGCACAGGCCC TGCTAGATGTGACACTTCTGGACATCAATGACCATGCCCCAGCTTTCAATCAAAGCCGATACCATGCTGT GGTATCTGAGAGTCTGGCCCCTGGAAGTCCTGTTTTGCAGGTGTTTGCATCTGATGCTGATGCTGGTGCC AACGGAGCTGTGACTTATGAGATCAACAGGAGACAGAGTGAAGGTGATGGACCCTTCTCCATTGATGCAC ACACAGGGTTTCTGAGGCTGGAGCGCCCACTGGACTTTGAACAAAGGCGAGTTCATGAACTGGTGGTACA GGCACGGGATGGTGGGGCTCACCCAGAGCTGGGTTCAGCTTTTGTGACAGTGCATGTGCGAGATGCAAAT GACAATCAGCCGTCCATGACTGTCATCTTCCTGAGTGCTGATGGCTCCCCCCGAGTGTCTGAGGCTGCCC CACCTGGACAGCTTGTTGCTCGAATCTCTGTGTCAGATCCAGATGATGGTGACTTCGCCCACGTCAATGT GTCCCTGGAAGGTGGAGAGGGCCACTTTGCTCTGAGCACCCAAGACAGTGTCATCTATCTGGTGTGTGTG GCTCGAAGGCTGGATCGGGAGGAGAGAGATGTCTATAACTTGCGAGTCACAGCCACGGACTCAGGCTCAC CCCCACTTAGGGCCGAAGCTGCTTTTGTGCTACATGTCACTGATGTCAATGACAACGCACCTGCTTTTGA CCGCCAGCTCTACCGACCTGAGCCTCTGCCTGAAGTTGCATTGCCTGGCAGCTTCGTGGTACGGGTGACT GCCCGGGATCCTGACCAAGGCACCAATGGTCAGATCACCTACAGCCTGGCTCCTGGCACTCACACTCACT GGTTCTCCATTGATCCCACTTCAGGCATCATCACCACAGCTGCCACACTGGACTATGAACTAGAGCCTCA GCCACAGCTTATTGTGGTGGCCACAGATGGGGGCCTGCCTCCTTTAGTCTCCTCGGCCACAGTTAGTGTG GCCTTACAAGACGTGAATGACAATGAGCCCCAGTTCCAGAGGACTTTCTACAATGCCTCGCTGCCTGAAG GTACCCAGCCTGGAACCTGTTTCCTGCAGGTAACAGCCACAGATGCTGACAGTGGCCCATTTGGCCTCCT CTCCTATTCCTTGGGTGCTGGGCTTGGAGCTTCTGGATCCCCCCCATTCCGCATTGATGCCCACAGTGGT GATGTGTGTACCACCCGGACCCTGGACCGTGATCAGGGACCTTCAAGCTTTGACTTCACGGTGACAGCTA TCGATGGGGGAGGCCTCAAGTCCATGGTGTACGTGAAGGTATTTGTGGCAGATGAGAATGACAACCCACC TCAGTTTTATCCACGGGAGTATGCTGCCAGTCTGAGTGCTCAGAGTACACCAGGCACAGCTGTGCTGAGG GTGCATGCCCATGACCCTGACCAGGGACCCCATGGGCGACTTTCCTACCATATCCTGGCTGGCAACAGTC CCCCACTCTTTGCATTGGATGCACACTCAGGGCTGTTGACAGTAGCCTGGCCCTTAGGCAGACGGGCTAA TTCTGTGGTGCAGCTGGAGATCGGGGCTCAGGATGGAGGTGGTCTACAAGCAGAACCCATTGCCCGAGTC AACATCAGCATTGTGCCTGGGACCCCCACACCACCTATATTTGAGCAACTACAGTATGTTTTTTCAGTGC CAGAAGATGTGGCACCCGGCACCAGTGTGGGCATAATCCAGGCACACAACCCACCAGGTCGCTTAGGGCC TGTGACCCTCACCCTATCAGGTGGGGACCCCCGAGGGCTCTTCTCCCTAGATTCACCCTCAGGGCTATTA AAAACACTTCGCCCTCTGGACCGGGAGCTCCTGGGACCTGTCCTAGAGCTAGAAGTTCGAGCAGGCAGTG GAACCCCACCAGTGTTTGCTGTGGCTCGGATCCGCGTGCTGCTGGATGATGTGAATGATAATTCTCCTGC CTTCCCTGCACCTGAAGACACTGTACTGTTGCCACAAAACACTGCTCCAGGGACTCCCATCTATACACTG CGCGCTCTAGACCCTGACTCAGGAGCTAACAGTAGAATCACCTTTAATCTACTTGCTGGGGGTGATGGAT TGTTCACTGTGGATCCCACCACAGGCCACGTCCGGCTCATGGGACCTCTGGGGCCTCCAGGGGGACCAGC CCATGAGTTGGAGGTGGAGGCCCGGGATGGAGGCTCCCCACCACGCACCAGCCATTTCCGACTTCGTGTG GTGATACAGGATTTGGGAATCCATGGGCTGGCTCCACGATTTGATAGCCCTACCTACCGTGTGGACTTAC CCTCGGGCACTACCACCGGAACTCAGATCTTGCAAGTGCAGGCCCAGGCACCAGATGGGAGCCCTGTCAC TTACCATCTTGCAGCAGATGGTGCCAGTAGCCCCTTTGGCCTGGAGTCACAGAGTGGGTGGCTGTGGGTA CGGACAGCACTAGACCGTGAGTCCCAGGAGTTATACACATTGAAGGTGATGGCTGTATCCGGTTCCAAAG CTGAATTGGGGCAACAGACAGGCACAGCCACAGTGAGGGTCATTATCCTCAACCAAAATGACCACAGTCC CCGCTTGTCTGAAGAGCCCACCTTTCTGGCTGTAGCAGAGAATCAACCCCCAGGGACCAGTGTAGGCCGA GTCTTTGCCACTGACAGAGACTCAGGTCCTAATGGACGTCTGACCTACAGCCTTCAACAGCTTTCAGAAG ACAGCAAGGCCTTCCGCATCCACCCCCAGACTGGAGAAGTGACTACACTCCAAACCCTGGACCGAGAGCA GCAGAGCAGTTTCCAGCTCCTGGTGCAAGTGCAGGATGGGGGTAGTCCACCTCGCAGTGCCACAGGCACT GTGCATGTCGCTGTGCTCGATCTTAATGACAACAGTCCCACCTTCCTGCAGGCTTCAGGGGCTGCTGGTG GGGGCCTCCCTATACAGGTACCAGATCGAGTGCCTCCAGGAACACTGGTGACAACTCTACAGGCCAAAGA TCCAGATGAGGGGGAGAATGGAACCATCCTATACACCCTAACTGGTCCTGGCTCTGAGCTCTTCTCTCTG CATCCTCACACCGGGGAGCTGCACACAGCAGCATCCCTGGTTAGAGCTGAGCGGCCTCACTATGTACTAA CTCTAAGTGCTCACGACCAGGGCAGCCCCCCTCGAAGTGCCAGCCTCCAACTGCTCGTGCAGGTACTTCC CTCGACTCGGGTGGTGGAGTCACCAGATCTCATAGAAGCTGACTCAGCGGCAACAGTGCCTGTAGTACTT ACGGTGACAGCAGCTGAGGGCCTTCGACCTGGATCCCTGTTGGGTTCGGTGGCCCCGCAGGAGCCAGCTA GTGTGGGCGTACTCACCTATACTCTGGTTGGTGGCGCGGACCCCGAGGGCACCTTCGCGCTGGACTCAGC CTCCGGTCGCCTGTACTTGGCGCGTCCCTTGGACTTCGAGGCTGGGCCAGCCTGGCGCGCGCTCACCGTG CGCGCCGAGGGGCCGGGAGGCGCAGGCGCGCGGCTGTTGAGAGTGCAGGTGCGTGTGCAGGACGAGAACG AGCACGCGCCAACTTTTGCGCGCGACCCCCTGGCACTGGCGCTACCTGAGAACCCCGACCCCGGTGCCAC GCTATACACTTTCCGCGCATCAGACGCCGATGGCCCAGGTCCCAACAGTGAGGTGCGATACCGCCTGCTG AGGCAGGAGCCCCCGGTGCCCGCCCTGCGCTTGGACGCGCGCACCGGGGCGCTCAGCGCTCCTCGTGGTC TGGATCGAGAGACCACACCTGCGCTGTTACTGCTCGTGGAAGCCACCGACCGGCCAGCCAATGCCAGCCG CCGCAGGGCAGCGCGCGTTTCAGCGCGCGTCTTTGTCACAGATGAAAATGACAACGCCCCGGTCTTCGCC TCCCCATCACGGGTGCGCCTCCCAGAGGATCAGCCCCCTGGGCCCGCAGCACTGCACGTGGTAGCTCGGG ATCCAGACCTGGGGGAGGCTGCCCGCGTGTCCTATCGCCTTGCGGCTGGAGGGGACGGCCACTTCCGGCT ACACGCGACCACTGGAGCGCTGTCCGTGGTGCGGCCTTTGGACCGTGAACAGCGAGCTGAGCATGTACTG ACAGTGGTGGCCTTGGACCATGGCTCCCCTCCACGTTCATCCACTCAGCTCCTGACTGTCAGTGTTGTTG ACGTCAACGACGAGGCACCGGCTTTCCCTCAGCAGGAGTACAATGTCATCCTTCGTGAGAACAGTCCCCC TGGCACTTCTCTGCTCACTCTGAAGGCAACTGACCCTGACCTTGGGGCCAATGGGCAAGTGACTTATGGG GGTGTCTCTGGCGAGAGCTTCTCTCTGGACCCAAACACTGGAGTTCTCACGACTCTGAGGGCTCTGGACC GGGAGGAGCAGGAGGAGATCTATTTGACAGTATATGCTCGGGATAGGGGCTTGCCACCCCTGTTAACGCA CATCACAGTGAGAGTGACTGTGGAAGATGAAAATGACCATACTCCAACTTTTGGGAACACCCACCTCTCC CTGGAAGTGCCTGAAGGCCAGGACCCCCAAACCCTAACCACACTAAGAGCCTCTGATCCAGATGGGGGAC TCAATGGACAGCTACAGTACCGCATCCTTGATGGGGACTCTTCAGGAGCCTTTGCACTAGATCTCACTTC TGGGGAGTTTGGCACCATGAGGCCACTAGACCGGGAGGTAGAGCCAGCATTCCAGCTGCAGATAGAGGCC AGAGATGGAGGCCAGCCAGCCCTCAGTGCCACCTTGCTTGTGACAGTGACAGTGCTGGATGCCAATGACC ATGCACCAGTCTTTCCTGTGCCTTCTTATTCTGTGGAGGTGCCTGAGGATGCACCTGTAGGGACCCTACT GCTGCAGCTACAGGCTCATGACCCAGATGATGGGGACAATGGCCGTGTGATGTACTACCTGGGTGCAGGT ACAGCGGGGGCCTTCCTGTTGGAGCCTACCTCTGGGGAACTGAGCACAGCTACAGCCTTGGACAGAGAAC ACTGTGCCAGCTATGCCTTTTCTGTGACCGCAGTGGATGGTGCAGCTGCTGGGCCTCTGAGCACCACAGT ACCAATCACCATCACAGTGCGTGACGTCAATGATCATGCACCCGCTTTCCCCACGAGTCCTCTCCGCCTC CGCCTACCCCGCCCAGGTCCTAGCCTCAACAAACCAACCCTGGCTCTGGCCACGCTTCGAGCTGAAGATC GAGATGCTGGTGCCAATGCCTCCATTCTATACCGGCTGGCAGGCACACCACCACCTGGCACCACTGTGGA CTCTTACACTGGTGAAATTCGTGTGGCTCGATCCCCCGTGGCTCTTGGACCCCAGGATCGTGTCCTCTTC ATTGTAGCTACTGACCTTGGACGCCCAGCTCGCTCCGCCACTGGTGTGGTCGTTGTTGGAATACAGGGAG AGCCTGAGCGTGGACCCCGCTTTCCCAGAACTAGTAGTGAGGCTGTGCTCCGTGAAAATGCACCACCAGG GACTCCAGTTATCTCCCCAAAGGCTGTCCATTCAGGAGGTTCAAATGGACCAATTACTTATAGCATTCTC AGTGGGAATGAGAGAGGGATATTCTCCATCCAACCTAGCACAGGAGCCATCACAGTTCGCTCAGCAGAGG GTCTTGACTTTGAAACAAGCCCACGGCTGCGGCTGGTGCTACAGGCAGAGAGTGGAGGAGCCTTTGCTTT CTCAGTGCTGACCCTGACCCTTCAAGATGCCAATGACAACGCTCCCCGTTTCCTGCGGCCTCACTATGTG GCTTTCCTGCCAGAGTCCCGACCCTTGGAAGGGCCCCTGCTGCAGGTGGAAGCCGATGACCTGGACCAAG GCTCTGGAGGACAGATTTCCTACAGTCTGGCTGCATCCCAGCCAGCACGGGGCTTGTTCCATGTAGACCC AGCCACAGGCACTATCACTACCACAGCCATCCTGGACCGGGAAATCTGGGCAGAAACACGGCTGGTACTG ATGGCCACAGACAGAGGAAGCCCAGCACTGGTGGGCTCAGCTACCCTGACAGTGATGGTCATCGATACCA ATGACAATCGTCCCACCATCCCCCAGCCCTGGGAGCTCCGAGTATCAGAAGATGCACTGTTGGGCTCAGA GATCGCACAGGTGACTGGGAATGACGTGGACTCTGGACCAGTGCTATGGTATGTGCTGAGCCCATCGGGG CCTCAGGATCCCTTCAGTATTGGCCGGTATGGAGGACGTGTCTCCCTCACGGGCCCCCTGGACTTTGAGC AGTGTGACCACTACCACCTACAGCTGCTGGCACATGATGGGCCTCATGAGGGCCATGCCAACCTCACAGT GCTTGTGGAAGATGTCAATGACAATGTACCTACCTTCTCACAAAGCCTCTACCAGGTAATGATGTTGGAG CACACCCCTCCGGGCAGCGCCATTCTCTCCGTCTCTGCCACCGACAGAGACTCGGGTGCCAATGGTCACA TATCTTACCATCTGGCTTCCCCTGCTGAGGGCTTCCGTGTCGACCCTAACAATGGAACTCTGTTCACAAC AGTAGGAGCAATGGCCTTGGGTCATGAGGGGCCAGGAGTGGTGGATGTGGTACTGGAAGCCCGAGACCAT GGGGCTCCAGGCCGGACAGCACAGGCCACAGTGCATGTGCAGCTGAAGGACCAGAATGACCATGCCCCAA GCTTCACATTGCCACACTACCGTGTTGCTGTGTCTGAAGATTTGCCCCCTGGTTCCACCCTGCTCACCCT GGAGGCCACAGATGCTGATGGAAGCCGCACCCATGCTACTGTGGACTACAGCATCATCAGTGGCAATCGG GGCCGAGTTTTCCAGCTGGAACCCCGTCTGGCTGAGGTCGGGGACGGTGTTGGGCCAGGTCCCCAAGCAC TGGGCTGCCTAGTGTTGCTTGAACCTCTAGACTTTGAAAGCCTGACACAGTACAATTTAACTGTGGCTGC AGCTGATCGGGGACAGCCACCCCGCAGTTCAGCCGTGCCAGTGACTGTCACTGTGCTGGATGTCAATGAC AACCCACCCGTCTTTACTCGAGCATCCTACCGTGTGACAGTACCTGAGGACATGCCTGTTGGAGCTGAGC TATTGCATGTGGAGGCCTCTGATGCTGACCCTGGACCTCATGGTCTTGTGCATTTCACTCTTAGTTCAGG TGACCCTTTGGGTCTCTTTGAGCTGGATGAGAACTCGGGTGCCTTGAGGCTATCCCGCCCTCTGGACTGT GAAACCCAGGCTCAACACCAGCTTGTGGTGCAGGCAGCTGACCCTGCTGGAACACACTTTTCTTTGGTAC CAGTGACAGTTGAGGTCCAGGATGTGAATGACCATGGTCCGGCCTTCCCATTGAGTCTGCTCAGTACTAG CCTTGCTGAGAATCAGCCTCCAGGCACTCTTGTGACCACACTACACGCGATTGATGGGGATGCTGGGACT TTTGGTAGACTCCGATATAGCCTGTTGGAGGCAGTGCCAGGGCCTGAGGGTCGTGAGGCATTTTCCCTGA ACAGCTCAACAGGGGAATTGCGGGCACGGGTGCCTTTTGACTATGAGCACACAGGAAGCTTCCGTCTACT AGTGGGTGCTGCCGATGCTGGGAATCTCTCAGCCTCTGTCACTGTGTCAGTACTGATTACTGGAGAAGAT GAATATGACCCTGTATTCTTGGCTCCATCTTTCCATTTCCAAGTGCCAGAAGGTGCCCAGCGAGGCCACA GCCTGGGTCACGTGCAGGCCACAGATGAGGATGGAGGTGCCGATGGTCTGGTGCTCTACTCGCTGGCTAC CTCTTCCCCCTATTTTGGTATCAACCAGACAACAGGTGCTTTGTACTTGCGAGTGGACAGTCGCGCACCA GGAAGTGGAACAACCACCTCTGGGGGTGGGGGCCGGACCCGACGTGAAGCACCTCGGGAGTTAAGGCTGG AGGTAGTAGCAAGAGGGCCTCTGCCTGGATCCCGGAGTGCCACAGTACCTGTGACTGTGGATATTACCCA CACTGCTCTAGGCCTGGCACCTGACCTCAACTTGCTATTGGTTGGGGCTGTGGCAGCCTCCTTGGGAGTT GTGGTGGTGCTTGCCCTGGCAGCTCTGGTCCTGGGCCTTGTTCGGGCGCGAAGCCGCAAGGCTGAGGCAG CCCCTGGCCCGATGTCACAGACAGCGCCGATAGCCAGTAGCTCTTTACAGAAACTGGGTCGGGAACCCCC CAGTCCACCACCCTCTGAACATCTGTATCATCAGACTCTTCCCAGCTACGGTGGCCCAGGAGCTGGAGGT CCCTACCCTCGTGGCGGCTCCCTGGACCCTTCACATTCAAGTGGCAGAGGCTCAGCAGAGGCTGCAGAGG ATGACGAGATCCGCATGATCAACGAGTTTCCCCGAGTGGCCAGTGTAGCCTCCTCCCTGGCTGCTCGAGG CCCTGACTCTGGCATCCAGCAGGATGCGGATGGATTGAGTGACACGTCTTGTGAGCCTCCTGCCCCTGAC ACCTGGTACAAGGGCAGAAAGGCAGGGTTGCTGCTGCCAGGCGCAGGAGCCACTCTGTACCGAGAGGAGG GGCCTCCAGCCACTGCTACAGCCTTCCTGGGGGGCTGTGGCCTGAGCCCTGCACCCGCTGGGGACTATGG CTTCCCAGCAGACGGGAAGCCGTGTGTAGCTGGAGCACTGACGGCCATTGTGGCTGGTGAGGAAGAGCTC CGTGGCAGCTATAACTGGGACTACCTGTTGAGCTGGTGCCCTCAGTTCCAGCCTCTGGCCAGTGTCTTCA CAGAGATTGCTCGGCTCAAGGATGAAGCTCGTCCGTGTCCTCCAGCTCCCCGTATTGACCCACCACCCCT CATCACTGCTGTGGCACACCCAGGAGCCAAGTCTGTTCCCCCAAAGCCTGCAAGCACAGCTGTAGCCCGG GCCATCTTCCCACCAGCCTCTCACCGCTCCCCTATCAGCCATGAAGGCTCCCTCTCCTCAGCTGCCATGT CCCCCAGCTTCTCACCCTCACTGTCTCCTTTGGCTGCTCGCTCTCCCGTTGTCTCACCATTCGGGGTAGC CCAGGGCCCCTCAGCTTCAGCACTCAGTACAGAGTCTGGCCTGGAGCCTCCGGATGACACAGAGCTGCGC ATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001162943
Insert Size	9876 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001162943.1, NP_001156415.1
RefSeq Size	10651 bp
RefSeq ORF	9876 bp
Locus ID	233651
UniProt ID	E9PVD3
Gene Summary	Calcium-dependent cell-adhesion protein. Mediates functions in neuroprogenitor cell proliferation and differentiation. In the heart, has a critical role for proper morphogenesis of the mitral valve, acting in the regulation of cell migration involved in valve formation (PubMed:26258302).[UniProtKB/Swiss-Prot Function]

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