Speg (NM_007463) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Apeg1; AW125581; BPEG; D1Bwg1450e; mKIAA1297; SPEGalpha; SPEGbeta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225472 representing NM_007463 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAAAGCCCGGGGCACGCGAGGCGAGGACGCCGGCACGAGGGCACCTCCTAGCCCCGGGGTGCCCC CGAAGAGAGCCAAGGTGGGGGCCGGCAGAGGGGTCCTCGTCACCGGGGATGGGGCCGGGGCGCCGGTCTT CCTGCGGCCCCTGAAGAACGCGGCGGTGTGTGCGGGCAGTGATGTAAGGCTGCGGGTAGTAGTGAGCGGG ACACCCCAGCCTAGCCTCAGCTGGTTCCGGGATGGGCAGCTCCTGCCTCCACCGGCCCCAGAGCCCAGCT GCCTGTGGCTGCGGAGCTGCGGGGCTCAGGACGCCGGCGTGTACAGCTGCAGCGCCCAGAATGAGCGGGG CCAGGCCTCCTGTGAGGCAGTGCTCACAGTGCTGGAGGTCAGAGACTCGGAGACTGCCGAGGATGACATC AGCGATGTGCCGGGGACCCAGCGCCTGGAGCTTCGGGATGACAGGGCCTTCAGCACCCCCACGGGGGGCT CTGATACCCTGGTGGGTACCTCCCTGGACACACCCCCGACCTCCGTGACAGGCACCTCAGAGGAGCAAGT GAGCTGGTGGGGCAGCGGGCAGACGGTCCTGGAGCAGGAAGCGGGCAGCGGGGGTGGCACCCGCCCCCTC CCAGGCAGCCCAAGGCAAGCACAGACAACCGGGGCCGGGCCACGGCACCTGGGGGTGGAGCCGCTGGTGC GGGCATCGCGAGCTAATCTGGTGGGCGCAAGCTGGGGGTCAGAGGATAGCCTTTCGGTGGCCAGTGACCT CTATGGCAGCGCGTTCAGTCTGTACAGAGGACGGGCGCTCTCGATCCACGTCAGCATTCCTCCGAGTGGA TTGCACAGAGAGGAGCCTGACCTTCAGCCTCAGCCAGCCAGTGATGCCCTTCGTCCACGCCCTGCCCTTC CACCTCCCTCCAAATCCGCTTTGCTTCCCCCACCATCTCCTCGGGTGGGAAAGAGGGCTCTGCCAGGACC TAGTACCCAGCCCCCAGCTACTCCCACTTCGCCCCATCGGCGTGCTCAGGAGCCCTCGCTGCCTGAGGAC ATCACCACCACGGAAGAGAAGCGAGGGAAAAAGCCCAAGTCATCCGGGCCGTCGCTGGCGGGCACAGTGG AGTCCCGGCCCCAGACGCCACTGAGCGAGGCTTCGGGTCGCCTGTCAGCATTGGGCCGCTCGCCCCGGCT GGTGCGCGCGGGGTCCCGCATCCTGGACAAGCTACAGTTCTTCGAAGAGCGGCGACGCAGCCTGGAGCGC AGCGACTCGCCGCCAGCGCCCCTGCGGCCCTGGGTGCCCCTGCGCAAGGCTCGCTCGCTGGAGCAGCCGA AGTCCGAGGGCGGTGCGGCGTGGGGCACACCCGAGGCCTCGCAGGAGGAGCTGCGGTCACCTCGGGGCAG TGTGGCAGAGCGGCGTCGCCTGTTCCAGCAAAAGGCGGCCTCGTTGGATGAACGCACGCGACAACGCAGT GCAACCTCGGACCTCGAACTCCGCTTCGCCCAGGAGCTGGGTCGCATCCGCCGATCTACGTCGCGGGAGG AGCTGGTGCGTTCGCACGAGTCCCTGCGTGCCACGCTGCAGCGCGCCCCATCCCCTCGGGAGCCCGGCGA GCCCCCACTCTTCTCCCGGCCTTCCACACCCAAGACCTCACGGGCTGTGAGCCCGGCTGCCACCCAGCCG CCGCCTCCTAGTGGTGCGGGCAAATCTGGGGACGAGCCTGGGAGGCCCCGAAGCAGAGGGCCGGTGGGCA GGACTGAACCGGGGGAAGGCCCGCAGCAGGAGATCAAGCGTCGGGACCAATTCCCGCTAACCAGGAGCAG AGCCATCCAGGAGTGCAGGAGCCCTGTGCCGCCCTACACCGCGGATCCCCCGGAGAGCAGGACAAAAGCC CCCTCCGGTCGCAAGCGGGAACCCCCTGCTCAAGCGGTGCGCTTTCTGCCCTGGGCCACTCCGGGAGTGG AGGACTCTGTTCTGCCCCAAACCTTGGAGAAGAATAGAGCGGGACCCGAGGCTGAGAAGAGGCTTCGCAG AGGACCTGAGGAGGATGGCCCCTGGGGGCCCTGGGACCGCAGAGGGACCCGCAGCCAAGGCAAAGGTCGC CGTGCTCGGCCTACTTCCCCCGAGCTCGAGTCCTCAGACGACTCCTATGTGTCCGCTGGGGAAGAGCCCC TGGAGGCACCCGTGTTTGAGATCCCTCTGCAGAATATGGTGGTGGCGCCAGGAGCTGACGTGCTACTTAA GTGTATCATCACCGCCAACCCCCCACCCCAAGTGTCCTGGAAAAAGGATGGGTCCATGTTGCACAGCGAG GGTCGTCTTCTCATCCGGGCTGAAGGTGAACGGCACACACTGCTGCTCAGAGAGGCCCAGGCTGCCGATG CTGGGAGCTACACAGCCACTGCCACCAACGAACTGGGCCAAGCTACCTGTGCTTCTTCACTGGCTGTGAG ACCTGGCGGCTCCACATCCCCTTTCAGCAGCCCCATCACCTCTGATGAGGAGTACCTGAGCCCCCCAGAG GAGTTCCCAGAGCCTGGGGAGACCTGGCCCCGAACCCCTACCATGAAGCTCAGTCCCAGCCAGGATCATG ATTCCTCCGACTCTTCTTCCAAGGCACCCCCAACGTTCAAGGTCTCACTCATGGACCAATCGGTGAGAGA AGGTCAAGATGTCATTATGAGCATCCGTGTGCAGGGAGAGCCCAAGCCTGTGGTTTCCTGGCTGAGGAAT CGACAGCCCGTGCGCCCAGACCAGCGGCGCTTTGCAGAGGAGGCCGAGGGTGGGCTCTGCCGCTTGAGGA TCCTGGCTGCTGAACGGGGCGATGCTGGTTTCTACACATGCAAGGCGGTCAACGAATATGGCGCTCGGCA GTGCGAGGCCCGCCTGGAGGTCCGAGCACACCCTGAAAGCCGGTCCCTGGCCGTGCTGGCCCCCCTGCAG GACGTGGACGTGGGGGCCGGGGAGATGGCGCTGTTTGAGTGCCTGGTGGCAGGTCCAGCTGACGTCGAGG TGGACTGGCTGTGCCGGGGCCGCCTGCTGCAGCCTGCGCTGCTCAAATGCAAGATGCATTTTGATGGCCG CAAGTGCAAGCTGCTGCTCACCTCTGTGCACGAGGACGACAGTGGTGTCTACACCTGCAAGCTGAGCACA GCCAAAGATGAACTGACCTGCAGTGCCCGGCTGACGGTTCGGCCGTCGCTGGCGCCTCTCTTCACCCGGT TGCTGGAAGATGTGGAGGTGTTGGAAGGCCGTGCTGCCCGCTTGGATTGCAAGATCAGCGGTACCCCGCC TCCCTCCGTTACCTGGACTCATTTTGGCCACCCCGTGAATGAGGGTGACAACTTGCGGCTTCAACAGGAT GGAGGTCTACACTCACTGCACATTGCCCGGGTGGGCAGTGAGGACGAAGGACTCTATGAAGTCAGTGCCA CCAACACTCATGGCCAGGCCCACTGCTCCGCCCAGCTGTATGTGGAGGAGCCTCGGACAGCTGCCTCTGG CCCCAGCTCGAAGCTGGAGAAGATGCCATCTATCCCTGAGGAGCCGGAGCACGGAGATCTAGAGAGGCTG TCTATTCCTGACTTCCTACGGCCACTGCAAGATCTGGAAGTGGGACTAGCTAAGGAGGCCATGCTGGAGT GCCAAGTGACCGGCCTGCCCTATCCCACCATCAGCTGGTTCCACAATGGCCACCGCATTCAAAGCAGTGA CGACCGACGCATGACACAGTACAGGGACATACATCGCCTGGTGTTCCCAGCTGTGGGGCCTCAGCATGCT GGTGTCTATAAGAGTGTAATCGCCAACAAGCTGGGCAAAGCGGCCTGCTACGCCCATCTCTATGTCACAG ATGTGGTTCCAGGCCCTCCAGATGGTGCTCCGGAAGTAGTAGCTGTGACCGGAAGGATGGTCACACTATC GTGGAACCCACCCAGGAGTCTGGACATGGCTATTGACCCAGATTCCCTAACATACACTGTGCAGCACCAG GTGTTGGGCTCGGACCAGTGGACGGCACTGGTCACAGGCCTGCGGGAGCCTGCGTGGGCAGCCACAGGGC TGAAGAAGGGGATCCAGCACATCTTCCGGGTCCTCAGCTCCAGTGGCAAGAGCAGCAGCAAGCCCTCAGC ACCGTCGGAACCCGTGCAGCTGCTAGAGCATGGCCCACCCCTGGAAGAGGCGCCTGCTGTGCTGGACAAG CAGGACATTGTGTATGTGGTAGAGGGCCAGCCTGCCTGTGTCACCGTCACTTTTAACCATGTGGAGGCCC AGGTTGTCTGGAGGAGCTGCCGGGGAGCCCTCCTCGAGGCGCGCACAGGTGTGTATGAACTGAGTCAGCC AGATGACGACCAGTATTGTCTGCGGATCTGCCGGGTGAGCCGACGAGACCTGGGACCCCTTACTTGCAGT GCCCGAAACCGCCACGGCACAAAGGCCTGCTCGGTCACCCTGGAGCTGGCAGAGGCACCTCGGTTTGAGT CTATCATGGAAGATGTGGAGGTGGGGCCTGGAGAAACCGCTCGCTTTGCTGTGGTAGTTGAGGGGAAACC ACTGCCAGACATCATGTGGTACAAGGATGAGGTGCTGCTGGCCGAGAGCAACCATGTGAGCTTCGTGTAT GAGGAGAACGAGTGTTCCCTAGTGCTGCTCAGCGCAGGAAGCCAGGATGGAGGCGTCTATACCTGTACCG CCCGGAACCTGGCAGGCGAAGTCTCCTGCAAGGCGGAGCTGTCTGTGCTTTCAGCTCAGACAGCCATGGA GGTCGAGGGGGTCGGAGAGGATGAGGAACACCGAGGAAGAAGACTTAGCGACTACTACGACATCCACCAA GAGATTGGCAGGGGTGCCTTCTCCTACCTGCGGCGTGTAGTGGAGAGGAGCTCTGGCCTGGAGTTTGCAG CCAAGTTCATCCCTAGCCAGGCTAAGCCTAAAGCATCAGCAAGACGCGAGGCGCGCTTGCTGGCCCGGCT CCAGCATGGCTGTGTGCTCTACTTCCACGAAGCCTTTGAGAGGCGCAGGGGACTTGTCATAGTCACGGAG CTCTGCACAGAAGAGCTTCTAGAGCGCATGGCCAGGAAGCCCACCGTGTGTGAGTCTGAGACTCGGACCT ATATGCGTCAAGTGCTAGAGGGGATATGCTATCTGCATCAGAGCCATGTGCTGCACCTGGATGTCAAGCC GGAGAACCTGCTTGTGTGGGATGGTGCAGGGGGTGAAGAGCAGGTGCGCATCTGTGACTTCGGGAACGCC CAGGAACTCACTCCGGGAGAGCCCCAGTACTGTCAGTATGGCACGCCAGAGTTTGTAGCACCTGAGATTG TCAACCAGAGCCCTGTGTCTGGAGTCACTGACATCTGGCCCGTGGGCGTCGTTGCCTTTCTCTGTTTGAC GGGCATCTCCCCATTTGTTGGGGAGAATGACCGGACAACACTCATGAATATCCGAAACTACAACGTGGCA TTTGAGGAGACTACGTTCCTGAGTCTGAGCAGGGAGGCCCGGGGCTTCCTCATCAAAGTGCTGGTGCAGG ATCGACTGAGACCTACTGCAGAAGAGACATTGGAACATCCTTGGTTCAAAACAGAAGCAAAGGGTGCAGA GGTGAGCACAGATCACCTGAAGCTCTTCCTGTCACGGAGGAGGTGGCAGCGCTCTCAAATCAGCTACAAG TGCCACCTGGTGCTGCGCCCCATTCCGGAACTGCTACGGGCTCCCCCGGAGCGGGTGTGGGTGGCCATGC CTAGAAGACAACCCCCGAGTGGGGGACTCTCATCCTCTTCAGACTCAGAAGAGGAAGAGTTGGAGGAACT TCCCTCAGTACCCCGCCCCCTGCAGCCTGAGTTCTCAGGTTCTCGGGTATCCCTCACCGACATCCCCACC GAAGATGAGGCTCTGGGGACCCCGGAAGCTGGGGCTGCCACCCCCATGGACTGGCAAGAGCAGGAAAGGA CTCCCTCTAAGGACCAGGAGGCCCCCAGCCCTGAGGCACTCCCCTCTCCAGGCCAGGAGTCCCCTGATGG GCCTAGCCCCAGGCGGCCAGAGCTCCGCAGGGGTAGCTCTGCTGAGAGTGCCCTGCCCCGAGTCGGGTCG CGGGAACCGGGCCGGAGCTTGCACAAGGCAGCATCCGTGGAGCTGCCGCAGCGCAGGAGCCCCAGCCCGG GTGCCACCCGCCTGACTCGGGGAGGCCTGGGTGAGGGCGAGTATGCCCAGAGGCTGCAGGCCCTGCGTCA GCGGCTGCTGAGGGGAGGCCCCGAGGATGGCAAGGTCAGCGGCCTCAGGGGACCCCTGCTGGAGAGCCTG GGGGGCCGTGCACGGGATCCCAGGATGGCAAGGGCTGCCTCCAGCGAGGCTGCCCCCCACCACCAGCCCC CTCCGGAGTCCCGGGGTCTGCAAAAGAGCAGCAGCTTCTCTCAGGGCGAGGCAGAGCCCCGGGGTCGGCA CCGCCGCGCGGGGGCACCCCTCGAAATCCCCGTGGCCAGACTTGGGGCTCGCAGGCTGCAGGAGTCTCCC TCCTTATCTGCCCTTAGCGAGACCCAGCCACCGAGCCCTGCGAGGCCCAGCGTCCCCAAACTCAGTATCA CCAAGTCCCCGGAACCTTCCGCTGTCACATCCAGAGACTCTCCCCAGCCCCCGGAACCTCAGCCTGTCCC AGAAAAGGTCCCTGAGCCCAAACCAGAACCAGTCCGAGCTGCAAAACCAGCACAACCCCCTTTGGCTCTG CAAATGCCAACGCAGCCTCTCACCCCCTATGCCCAGATCATGCAGTCGCTCCAGCTGTCCAGTCCCACCC TGTCCCCACAGGACCCCGCTGTGCCTCCATCAGAGCCCAAGCCCCACGCGGCTGTGTTTGCCAGGGTGGC TTCCCCACCACCCGGAGTCTCTGAGAAGCGCGTGCCTTCGGCCAGAACTCCCCCAGTGCTAGCCGAGAAA GCCCGAGTCCCCACGGTGCCCCCCAGGCCAGGCAGCAGCCTCAGCGGCAGCATCGAAAACCTTGAATCAG AGGCTGTGTTCGAGGCTAAGTTCAAACGCAGCCGCGAATCGCCACTGTCGCGGGGCCTGAGGCTACTGAG CCGCTCGCGCTCGGAGGAGCGCGGTCCCTTCCGTGGGGCGGAGGACGACGGCATATACCGGCCCAGTCCG GCAGGAACGCCGCTAGAGCTGGTGCGACGGCCCGAGCGCTCGCGCTCCGTGCAGGACCTCAGGGTAGCCG GAGAGCCGGGCCTTGTGCGTCGCCTTTCGCTGTCCCTGTCGCAGAAGCTGCGGCGGACACCGCCGGGACA GCGACACCCTGCCTGGGAGTCCCGCAGCGGAGACGGGGAGAGCTCCGAGGGCGGCAGCTCAGCTCGGGCA TCCCCAGTGCTGGCGGTACGCAGGAGACTCAGCTCCACGCTGGAGCGTCTGTCCAGCCGTTTGCAGCGCA GCGGCAGCAGCGAGGACTCCGGGGGCGCCTCGGGTCGCAGCACGCCGCTGTTTGGACGGCTGCGCAGGGC TACTTCGGAAGGGGAGAGCCTGCGACGCCTCGGTGTTCCGCACAATCAGCTGGGCTCTCAGACTGGTGCC ACTACACCTTCCGCTGAGTCTCTGGGCTCAGAGGCCAGTGGCACATCGGGTTCTTCAGCTCCTGGAGAAA GCCGAAGCCGGCACCGGTGGGGCCTTTCCAGGCTGCGCAAGGACAAGGGATTATCTCAGCCAAACCTCTC CTCCAGTGTCCAAGAGGACTTGGGTCACCAGTATGTGCCCAGTGAGTCAGATTTCCCCCCAGTCTTCCAC ATCAAACTCAAGGACCAGGTGCTGTTGGAGGGGGAAGCAGCCACCCTGCTCTGCCTACCCGCAGCCTGCC CAGCACCCCGCATCTCCTGGATGAAAGATAAGCAGTCCCTGCGTTCAGAGCCCTCAGTGGTCATTGTGTC CTGCAAAGATGGACGGCAGCTGCTGAGCATACCCCGGGCCGGCAAGCGGCATGCTGGGCTCTATGAGTGC TCAGCCACCAATGTCCTGGGCAGCATTACCAGCTCCTGTACTGTGGCCGTGGCCCGCATTCCAGGGAAGC TAGCTCCTCCAGAGGTGCCCCAGACCTACCATGACACAGCACTAGTAGTGTGGAAGCCAGGAGACGGTCG GGCACCTTGCACATACACTCTGGAGCGGCGGGTGGATGGGGAATCTGTCTGGCACCCTGTGAGCTCAGGC ATCCCCGACTGTTATTACAATGTGACTCAGCTGCCTGTCGGCGTGACCGTGCGGTTCCGTGTGGCCTGTT CCAATCGTGCTGGGCAGGGGCCCTTCAGCAACCCTTCTGAGAAGGTCTTCATCCGAGGCACTCCAGATTC TCCTGCTCAGCCAGCTGCTGCTCCCCGAGATGCCCCTGTTACCTCAGGGCCAACCAGGGCCCCACCGCCT GACTCTCCTACCTCACTGGCCCCCACCCCCGCTCTTGCGCCCCCAGCCTCCCAGGCATCCACTCTCAGCC CCTCAACTTCCTCCATGTCTGCCAACCAGGCCTTGTCCTCACTCAAGGCTGTGGGTCCACCACCCGCAAC CCCTCCACGAAAGCACAGGGGCCTTCTGGCCACCCAGCAAGCAGAGCCATCCCCACCCAGTATCGTGGTC ACCCCAAGTGAACCCAGGTCTTTTGTCCCTGACACTGGAACCCTGACCCCAACCTCTAGTCCTCAAGGGG TTAAACCAGCACCTTCCTCAACTTCCTTGTATATGGTGACTTCCTTCGTGTCTGCACCTCCAGCCCCCCA GGCCCCAGCCCCTGAGCCCCCTCCTGAGCCCACCAAGGTGACTGTGCGGAGCCTCAGCCCAGCCAAGGAG GTGGTCAGCTCCCCAACTCCTGAGAGCACCACTCTTCGGCAGGGTCCCCCTCAGAAACCCTACACCTTCC TGGAGGAGAAGGCCAGGGGCCGCTTTGGCGTTGTGCGGTCATGCCGGGAGAATGCTACGGGCCGAACGTT TGTCGCCAAGATCGTGCCGTACGCCGCGGAGGGCAAGCGACGCGTGCTGCAGGAATACGAGGTGCTTCGG ACGCTGCACCACGAGCGGCTCATGTCCCTGCACGAGGCCTACATCACCCCTCGGTACCTCGTGCTTATCG CTGAGAGCTGTGGCAACCGGGAACTCCTCTGTGGGCTCAGCGACAGATTCCGGTATTCAGAAGATGACGT GGCCACCTATGTGGTGCAGCTGCTACAAGGCCTGGACTACCTCCACGGCCACCACGTGCTACACCTGGAC ATCAAGCCGGACAATCTACTGTTGGCCGCTGACAATGCCCTTAAAATTGTGGACTTCGGCAGTGCTCAGC CCTATAACCCTCAGGCCCTGAAGCCACTTGGCCACCGCACAGGCACTCTGGAGTTCATGGCTCCTGAGAT GGTGAAGGGAGACCCCATTGGCTCTGCCACGGACATCTGGGGAGCGGGTGTGCTCACCTACATCATGCTT AGTGGGTACTCCCCATTCTATGAGCCAGACCCCCAGGAAACAGAGGCTCGGATTGTTGGGGGTCGCTTTG ATGCCTTCCAGTTGTATCCTAACACATCCCAGAGTGCCACCCTCTTCTTGAGAAAGGTCCTCTCAGTACA TCCCTGGAGCCGGCCCTCTCTGCAGGACTGCTTGGCCCACCCATGGCTGCAAGATGCCTACCTGATGAAG CTGCGCCGCCAGACACTCACCTTCACCACCAACCGGCTCAAGGAATTCCTGGGCGAGCAGCGGCGACGTC GGGCTGAGGCTGCTACCCGTCACAAGGTGCTGCTCCGCTCCTACCCTGGCAGCCCCTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_007463
Insert Size	9789 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007463.4, NP_031489.4
RefSeq Size	10795 bp
RefSeq ORF	9789 bp
Locus ID	11790
Gene Summary	This gene encodes a protein with similarity to members of the myosin light chain kinase family. This protein family is required for myocyte cytoskeletal development. Studies have determined that a lack of this protein affected myocardial development. Multiple alternatively spliced transcript variants that encode different protein isoforms have been defined. [provided by RefSeq, Mar 2010] Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1). This variant corresponds to variant SPEGbeta described in PubMed ID: 10973969. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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