Ep400 (NM_029337) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700020J09Rik; AU023439; mDomino; mKIAA1498; p400
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225450 representing NM_029337 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCATGGCAGTGGTCCTCAGAATGTCCAACATCAGCTGCAGAGGTCTAGGTCCTTCACTGGGAGTG AAGAGGAGCAGCCAGCCCATCCCAACTTACCCCCATCTCCTGCAGCGCCTTTCGCTCCATCAGCCAGTCC ATCTGCACCCCAATCCCCTGGGTACCAGATACAGCAGCTGATGAGCAGAAGTCCTGTGGCCGGGCAGAAT GTGAACATCACACTGCAGAATGTTGGCCCAGTTGTGGGAGGAAACCAGCAGATCACACTGGCCCCCCTGC CACTGCCCAACCCCACTTCTCCAGGTTTTCAGTTTGGTGCCCAGCAAAGGCGCTTTGAGCATGGCTCTCC ATCATACATTCAAGTTACTTCTCCGATGTCGCAGCAGGTCCAGACTCAAAGCCCCACACAGCCTAGCCCT GGGCCAGGGCAAACCCTGCAGAATGTGCGTGCAGGTGCTCCAGGTCCTGGGCTGGGCATATGCAGCAACA GCCCCACTGGAGGCTTTGTGGATGCCAGTGTGCTTGTGAGGCAGATCAGCTTGAGTCCATCAAGTGGTGG TCACTTTGTATTTCAGGAGGCACCAGGCCTGACTCAGATGGCCCAGGGAGCCCAGGTTCAACTCCAGCAT TCAGGAGCTCCCATCACTGTCCGAGAACGGAGACTCTCCCAACCTCATGCACAGTCGGGAGGCACCATCC ACCATCTAGGGCCTCAGAGCCCTGCAGCTGCAGGAGGCACTGGACTTCAACCTCTGGCCAGCCCAAACCA CATCACCACGGCCAGCCTGCCACCCCAGATCAGCAGCATTATTCAGGGCCAGTTGATACAGCAACAGCAG CAGGTGCTTCAGGGACAGCCAATGAACCGATCTCTGGGATTTGAGAGGACACCTGGTGTGCTGCTTCCTG GCGTGGGAGGGCCTTCAGCATTTGGGATGACATCCCCACCACCACCCACCAGCCCTTCCAGAACCACCAT GCCTCCAGGCCTTTCCAGTGTGCCTCTTACATCTATGGGAAGCTCAGGAATGAAGAAGGTTCCCAAGAAG TTGGAGGAGATCCCCCCAGCCTCCCAAGAGATGGCACAGATGAGGAAGCAGTGTCTGGACTACCACTACA AGGAAATGGAGGCACTCAAGGAAGTCTTCAAGGAGTACCTGATTGAACTGTTCTTCTTGCAACATCTACA GGGAAACATGATGGATTTCTTGGCTTTTAAAAAGAAGCACTATGCCCCTTTACAAGCGTATCTTAGGCAG AATGATTTGGACATTGAAGAGGAGGAGGAGGAAGAGGAAGAGGAGGAAGGAAAATCTGAAGTTATCAACG ATGAGCACCAATCCCTGACTGGGAGCCTAGTGGTGGGGCCTGGCAGTGCCACAGAAGCCGACCCCTTTAA GAGGCAACAGGTGATGCCACCCACAGAGCAGTCTAAGAGACCTCGTCTTGAAGTGGGTCATCCAGGGGTA GTTTTCCAGCATCCAGGGGTGAATGCAGGAGTCCCTCTGCAGCAGTTAATGCCAACAGTCCAAGGAGGGA TGCCCCCAACTCCTCAGGCCACACAGCTCACTGGACAGAAGCAGAGTCAGCAGCAGTACGACCCTTCCAC AGGGCCTCCTGTGCAGAACGCCGCCAGCTTGCATACCCCACCACCACAGCTTCCTGCGAGGCTGCCTCCG GCCAGTGTCCCAGCCACAGCCCTCCCTTCCACCTTACAGTTTTCACAGCAGTCACAGATGGTGGAGGCTT CAACTCAGCTCCAAATCCCTGTGAAGACTCAACAGCTAAATGCCCCCATCCCTGCCCCCCTGCCCAGCCA GCTCCCTGCTCCTTCTTCACAGCCCGCCCAGCCGGCCCTACATGTTCCGATGCCCGGGAAGGCGCAGATG CAGACATCTCAGCTTTCCTCCCAGACCCAGACAGTGGCATCGACCAGGCCCCCCTTGGACTCTGCCCAGC CCTGCCAGCGGTCTCTGCCCACTTCCTCCTCTTCCTCATCCCTTGTACCTGTGAGTGGCTCAGGTCCTGG ACCCTCACCTGCTCGTTCTTCACCAGTGAACAGACCCTCTTCTGCTACCAACAAGGCTCTGTCTCCCATC ACTTCTCGGTCTCCAGGGGTGGCAGTATCAGCTCCTCCTAAGCCACAGAGTCCTGCTCAGAATGCAGCCT CATCCCAGGACGGTTCTCAGGATAAACTGGCAGAACAAATAACACTGGAAAACCAGATCCATCAGCGAAT AGCAGATTTAAGGAAGGAAGGCTTGTGGTCCCTAAGGCGGTTGCCAAAGCTTCAAGAGGCCCCACGCCCG AAATCTCACTGGGACTATTTGCTGGAAGAGATGCAGTGGATGGCCACAGACTTTGCTCAAGAGCGGAGGT GGAAGTTGGCTGCTGCTAAGAAGCTTGTCAGAACTGTAGCCCGGCATCATGAGGAAAAGAAACTTCGTGA GGAAAGAGGGAAGAAGGAAGAACAGAGCAGACTGAGGCGCATTGCTGCCACTACTGCCAGAGAAATAGAA TATTTTTGGTCAAATATTGAACAGGTTGTGGAAATAAAATTACAAGTAGAATTAGAAGAGAAAAGAAAAA AAGCCTTAAATTTACAGAAAGTTTCCAGAAGAGGTAAAGAGTCCAGATTAAAGGGATTTGATACATCACC AGAACACTCTCTGGATCTAGGAATATCTGGAAGGAAAAGAAAAGCTAGCACATCTTTGACTGATGATGAA GTGGAAGATGAAGAAGAGACCATTGAAGAGGAGGAAGCACACGAAGGGCTTGTAGACCACCACACAGAAC TTACTAATCTGGCCAAAGAAGCTGAGTTGCCCTTAATCGATTTGATGAAGTTGTATGAAGGTGCCTTTCT GCCAAATTTTCAGTGGCCACAGCCTGAACCTGATCATGAAGAGTCAAGTGGGGAAGAAGATGTAGAAGAC TGTCCTAGTGACAGGGAGAGCCGGAGGGACTCCGTTCTCATTGACTCACTCTTCATCATGGATCAGTTTA AAGCTGCAGAGAGAATGAGCATTGGAAAATCCAACACCAAGGACATCACAGAAGTTACTGCTGTGGCTGA AGCCATCCTCCCTAAGGGCAGTGCCCGAGTCACAACTGCGGTGAAGTTTAGTGCTCCATCTTTGTTGTAT GGTGCTCTCCGAGACTATCAGAAGATAGGCCTGGACTGGTTGGCCAAGCTATACCGGAAGAATCTCAATG GCATATTGGCTGATGAAGCAGGGCTTGGGAAAACTGTGCAGATCATTGCTTTTTTTGCTCACCTTGCCTG TAATGAAGGTAACTGGGGCCCTCATCTTGTTGTCATGAGGAGTTGTAACATACTCAAGTGGGAACTCGAG TTGAAACGCTGGTGTCCTGGCCTCAAAACTCTTTCTTATGTTGGCAGCCACAGAGAACTCAAAGCAAAGA GACAGGAGTGGACCGAGCCCAATAACTTCCACATCTGCATCACGTCCTATAAGCAGTTCTTCAGGGGCTA CACTGCCTTCTCAAGGGTGCACTGGAAATGCCTTGTTGTTGACGAAATGCAGAGGGTGAAGGGCATGACT GAGCGGCACTGGGAAGCCATCTTCAAGCTGCAGAGCCAGCAGCGCCTGCTTCTGATCGATGTTCCTCTGC ACAACACTTTCCTGGAGCTCTGGACCATGGTGCACTTCCTCATTCCTGGGATCTCCAGGCCTTATCTGAG CTTTCCTCTGAAAGCCCCCAATGAGGAAAACCAGGATTACTATCACAAAATGGTCATAAGGTTACACAGG GTCACACAGCCATTTATTTTGAGGAGAACCAAGAGAGATGTGGAGAAACAGCTAACCAGGAAATATGAGC ATGTTCTGAAGTGCCGCCTTTCCAGTCGTCAGAAGGCCTTATATGAGGATGTCATCCTACAGCCTAGGAC TCAGGAAGCTCTGAAGAGTGGACATTTTGTCAGTGTCTTGAGTGTTCTGACACGGCTACAGCGAATCTGC AACCATCCTGGACTAGTAGAGCCACGGGTACCAGGCTCCTCCTTTGCAGCAGGCTCTCTGCAGTATAAGT CAGCCTCTCTAATTCTCAGAGTTCTGGAGAGAGAGTTCTGGAAGGAAACCGACCTTTCTATATTTGATCT CATTGGGCTAGAAAATAAAATCACTCGCCATGAAGCTGAATTACTGTGTAAGAAAAAAGTGACTCGGAAA CTCATGGAGGAAGTCTTTGCTTCACCTCCTCCGTCAGCCCGACCAGCAGCAGTGAAGCTCAAGGCCAGCA GGTTATTTCAGCCTGTGCAATATGGCCAGAAGCCTGAGGGTCGCACTGTGGCATTCCCCAGCACACATCC ACCTCGGATGGCAAACACCAACACATCCACCGCCACTCCACAGGGCCAGGTTCGAGGACGGCCGCCGATT GCTACTTTCTCTGCCAACCCAGATACAAAAGGTGGGGAAGTAGTGAAAATAGCTCAGCTGGCGTCTATTG CAGGGCCACAGAGCCGTGTGGCCCAGCCAGAGACACCTGTGACACTACAGTTCCAAGGGAACAAATTCAC CTTGTCACATAGCCAGCTGCGGCAGCTCACAGCAGGCCAGCCTCTGCAGCTGCAAGGTAGTGTACTCCAG ATCGTGTCAGCCCCTGGACAGCCCTACCTTCGAGCCCCTGGCCCTGTGGTGATGCAGACTGTGTCTCAGG CAGGTGCTGTGCATAGCACCCTGGGGAGCAAGCCCCCAACCAGTGGCCCCAGCCCTGCACCCTTGACCCC ACAAGTTGGTGTTCCGGGTCGAGTAGCAGTGAGTGCTATGGCTGTTGGAGAACCTGGCCTGGCTTCCAAA CCAGCTTCTCCTGCTGCAGGGCCAACCCAGGAGGAGAAGAGCCGACTTTTGAAGGAACGGTTGGATCAGA TTCATTTTATCAATGAACGACGTTGTTCCCAGGCTCCAGTGTATGGCAGAGACTTGCTGAGGATTTGTTC CCTTCCTGGAAGAAGGAAGAGGCCTTTGTGCTGGTCTTTGGATAGCAACTTTGGGAAGGGTCCTAAAGGA GTAAACTATGACATGTCACTCTCAAAGAGTGAAGGTGATCTGATTTTGACATTGAGTCAAGAGTCTCTTC AAGATGTTCTTGGCAGGGTGGCCTGTGTGATTCCTCCAGTGGTGGCTACACCACCCTCCCTGTGGGTCGC GAGGCCGCCCTCATTGTATAGCAGCAGGTTGAGAGCTCTGAGGCAGTGCCTGAGGGAGCACACCGGTCCT TACCACCGGCAGCTGCAACAACTAACAGCACTGCGCTCACTGCAGTTCCCGGAGCTGAGGCTGGTGCAGT TTGACTCAGGGAAGTTAGAAGCTTTAGCAATCCTGCTACAGAAACTAAAATCTGAAGGACGTCGGGTACT GATTTTATCTCAGATGGTTCTTATGCTAGACATTTTAGAGATGTTCTTGAACTTCCATTATCTCACTTAT GTACGGATTGATGAAAATGCCAACAGTGAACAACGACAGGAGCTGATGAGGAGCTTCAACAGAGACAGGC GGATTTTCTGTGCCCTTCTCTCTACCCATAGTCGTGCCACTGGCATAAACCTTGTTGAGGCTGACACTGT GGTGTTTTATGACAATGACCTAAATCCAGTTATGGATGCCAAAGCCCAGGAGTGGTGTGATAGAATTGGG AGGTGCAAGGACATCCACATATACAGGCTTGTCAGTGGCAATTCTATTGAAGAGAAATTGTTAAAAAATG GAACTAAAGATTTGATCCGTGAAGTAGCTGCTCAAGGAAATGACTATTCAATGGCCTTCTTAACGCAGCG AACTATCCAGGAGCTGTTTGAAGTCTACTCACCTATGGATGATACTGGCTTTCCTGTGAAAGCTGAGGAA TTCGTGGTGCTTTCTCAGGAACCATCTGTCAGTGAAACTATCGCACCCAAGATTGCAAGACCTTTCATAG AGGCCCTCAAGAGCATCGAGTGTCTGGAGGAAGATGCACAGAGGTCCACAGAGGAAGCTGTGCCTGGATC CAGCAGTGTAGCTGTCTCCTCAGACTCAGATGGCTCTAGATATGACGAGGAACCATCACAGCTGGAGGAG CTGGCTGACTTTATGGAACAGCTTACTCCAATTGAAAAATATGCATTGAATTACTTGGAATTGTTCCATA CGACCACTGAACAAGAAAAAGAGAGAATCAGTGAGGATTTGGTAATGGCGTCAATGAAGGACTGGGAAAC CCGAAATGCTAGAGCCCTGCAGGAAAGGGAGGCCCGGCTACAGCTGGAGCAGGAGGAGGCAGAGCTCCTC ACCTACACTCGGGAGGACGCCTATACTATGGAGTATGTCTATGAAGATGCTGATGGACAAACAGAAGTCA TGCCGCTCTGGACACCACCCACCCCTCCTCAGGATGACAATGATATCTACATTGACTCAGTTATGTGTCT CATGTATGAGACCACACCCATCCCAGAAGCTAAGCTGCCTCCTGTTTATGTGAGGAAGGAGCGCAAGCGG CACAAAACAGACCCCTCAGCTGCAGGCCGGAAGAAGAAGCAGCGGCATGGTGAGGCAGTTGTCCCGCCCC GGTCCTTGTTTGACCGGGCAACACCTGGTATGTTGAAAATCCGGCGTGAAGGCAAAGAGCAGAAGAAGAA CCTGCTGCTGAAGCAGCAGACACCTTTTGCCAAGCCTCTGCCCACCTATGTCAAGTCCTCAGGGGAACCT GCCCAGGACAGTCCTGACTGGCTCATTGGCGAGGACTGGGCCTTGCTACAGGCTGTAAAGCAGTTGCTTG AGCTGCCCTTGAACCTCACGATTGTGTCACCTGCCCACACTCCTAATTGGGACCTTGTCAGTGATGTTGT CAACTCCTGTAGTCGAATCTACCGTTCTTCCAAGCAGTGCCGGAATCGTTATGAGAACGTCATCATTCCC AGGGAGGAGGGGAAGAGTAAGAACAATCGTCCTCTCCGCACAAGCCAGATTTATGCTCAGGATGAAAACG CCACTCACACCCAGTTGTACACGAGCCACTTTGAGCTGATGAAAATGACAGCTGGCAAGAGAAGCCCCCC AATCAAGCCTCTGCTTGGCATGAATCCCTTTCAGAAGAACCCCAAGCATGCCTCTGTCCTGGCAGAAAGT GGAATCAACTATGACAAGCCGCTGCCTCCCATTCAAGTTGCATCTCTCCGTGCAGAGCGAATTGCAAAAG AGAAAAAGGCTTTGGCTGATCAGCAGAAGGCACAGCAGCCACCAGTGACACAGCCGCCCCCTCAGCAACA ACAGCAGCAGCAACAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAACCCCCGCCACCTCCACAGCAGCCA CCACCACCAGTGCCGCAGCCACAGGCAGCAAGCAGTCAGACACCAGCAGGGCAGCCTGCTGTCCAGCCCC AGCCCCAGCCCCAGGTCCAGACCCAGCCACAGCCTGTGCAGCCACAGTCAAAGGGGCAGCCCACAATGAC GACTGTGGGCAGTGCTGCTGTGCTGGCAGGAACCATTAAAACATCAGTCACTGGAACAAGCATCCCTACA GGGACTGTAAGTGGAAATGTGATTGTGAACACAATAGCTGGTGTCCCTGCTGCTACTTTCCAGTCCATTA ACAAGCGTCTGGCATCACCTGTGGCTCCTGGAACTTTGACTACGTCTGGAGGTTCTGCTCCTGCTCAGGT GGTCCACACTCAACAACGAGCAGTTGGTTCCCCAGCCACTGCCACCACTGACCTGGTATCCATGACAACT ACTCAAGGTGTTCGAGCAGTTACTTCAGTGACGGCCTCAGCTGTGGTCACTACCAACCTGACTCCAGTGC AGACTCCAACCCGTTCCTTAGTAACCCAGGTGTCTCAAGCCACAGGGGTTCAGCTACCTGGAAAAACCAT CACCCCTGCTGCTCATTTCCAGCTTCTTAGGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAACAG CAGACTTCCCAGGTGCAAGTTCCACAGCTCCAGAGCCAGGCCCAATCTCCTGCACAGATCAAAGCTGTGA GCAAGCTAGGACCGGAACACATCATCAAGATGCAGAAACAGAAGATGCAGCTGCCACCACAGCCCCCACC ACCGCAGGCCCAGCCAGGTCCCCCACAGCAACCAGCGCAAGTGCAAGTACAGACTCCACAGCCCCCACAG CAACAGCAGAGTCCTCAGCTTACCACAGTCACGGCCCCACGGCCTGGGGCTTTGCTCACAGGCACCACAG TGACCAACCTTCAGGTGGCCCGGCTTACCCGAGTCCCTACTTCTCAGCTGCAGGCACAAGGGCAGATGCA AACTCAGACGCCCCAGCCTGCTCAGGTGGCCTTGGCAAAGCCTCCGGTGGTATCTGTCCCAGCAGCTGTG GTCTCCTCGCCAGGGGTCACGACCCTGCCTATGAACGTTGCAGGGATCAGCGTGGCAATTGGACAGCCAC AAAAAACAGCAGGGCAGACGGTCGTGGCCCAACCCGTCAATGTCCAACAGCTGCTGAAGTATAAGCAGCA GACAGCCGTCCAACAGCAGAAGGCCATCCAGCCACAAGTTGCCCAGGGCCAGGCTGCTGTTCAGCAGAAG CTGACCACCCAACAGATCACTACTCAGGGCCCGCAGCAGAAGGTCGCTTATGCTGCCCAGCCAGCCCTTA AAACCCAGTTTCTGACCACACCCATCTCCCAGGCCCAGAAACTGGCCGGGACTCAGCAGGTTCAGACTCA GATCCAGGTTGCAAAACTTCCTCAAGTTGTGCAGCAACAAACACCTGTGGCCAGTATACAACAAGTTGCA TCAGCTTCTCAGCAGGCTTCTCCACAGACGGTGACGCTCACACAGGCAACGGCAGCAGGGCAACAGGTAC AGATGATCCCAACGGTGACTGCAACAGCCCAGCTGGTGCAGCAGAAGCTCATCCAGCAGCAGGTGGTGAC GACAGCCTCAGCATCACTGCAGACCCCTGGTGGCCCAAGCCCAGCACAGCTTCCAGCCAGCTCTGACAGC CCAAGCCAGCAGCCAAAGTTGCAAATGAGAGTTCCTGCTGTGAGGCTGAAGACGCCCACCAAACCTCCAT GCCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_029337
Insert Size	9108 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_029337.2, NP_083613.2
RefSeq Size	10798 bp
RefSeq ORF	9108 bp
Locus ID	75560
UniProt ID	Q8CHI8
Gene Summary	Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome (By similarity). Regulates transcriptional activity of ZNF42.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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