Wdfy4 (NM_001146022) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gm18810
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225448 representing NM_001146022 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTGAAGATCTTTCAAAGACTGAGGACAGACCCGAGGACCCAGGTTTCCAAAATGAAGGGCAAT CCCCTGCTGTGAAGCCCAGTTTTTCACTAGAAGGGCAGTCGCCCGGCCCCAGTGTCCTCTGGGATATGCT TGAACAGAAATTCCTGGACTATCAGCAGCTAATGCCCAGGAACCCAGAAGAACGTCGCAAGAACTTGCTG AGCCTTCTCCCCCTCTTCCTAAAGGCTTGGGAACATTCTGTGGGTATTATCTGCTTTCGAAGTCTCCAAA GGCTGGCAGAAGATGTGTCTGACCAACTTGCCCAGGAAATACAACAGGCACTTGCTGGGAAGCCCGCAGA GCAAGCCCGGGCCGCAGCTGGGCAGCTACTGCAGTGGAAGAGTGACGCGGATCAGGATGGCAACCTGCTC CTAAAGTCAGTGTATGTGCTCACGGGGACAGACTCGGAGACACTGGGCAGAGTGGTGGATTCAGGGCTTC CAGCCCTGCTCCTTCAGTGCCTGTATCTCTTCTTCGCTTTTCCTGTGGAAAAGGATGACCTTTTGGAGAG CGATGTTCAAGGGCAGAGGATGTTTGTGCAGATGTTGTTAAACATTTGCAGCGAATCTCAGGGTCTGGAG GGACTTCTCTCAGGAAGCGAGCTCCAGTCTCTACTGATCGCCACCACCTGTCTGAGGGAACACAGCTGCT TGTTCTGGAAACAGCCTACCTTCTGTGTGCTGCGGGCGATCTCCAAGGCTCAGAGCCCCAGCGTCATCCA ATATCTGCGAACTGCAGACTGTGTCCGGCTCTCAGTTCAGAACCTCTCCAAGTTGGCAGACACTCTCCCC GCGCCGGAGGTGAGCGAGGCTGTGAGCCTCATCCTGAACTTTGTGAGAGACTCCTACCCCATCTCCTCTG CGCTGCTCCTGGAGTTTGAGAACGGGGAGGGCTACCCTCTTCTCCTCAAGGTACTGCTGCGGTATAACGG GCTGACCCAGGGTGTGGTGGAGCCCCATCTGGAGGAGCTCATCGAGCTGGTGATGTGGCTGACAACCTGT GGGAGGTCAGAGCTGAAGGTGTTTGACAGCGTCACTTATCCTCAGCTTGAAGGCTTCAAGTTCCATCAGG AGGCTTCTGGAGTGACTGTTAAGAATCTCCAGGCCTTCCAGGTCCTGCAGAATCTTTTCCACAGAGCCAG TGACTCTGTCCTTTGCATTCAAGTGCTTTTGGCCATCAAGACCATGTGGGCCTGGAATCCTCGCAATTTC TTCCTGCTGGAGTGGACCCTGCAACCCATCTCACAGTTTGCAGAGATCATTCCCCTGAAGCCAACCCCAG TGCAGGAGCACTTTTTCCAGCTGTTGGAGACTCTGGTGTTCAAGCTGCTCTATGTGCCCCACGAGGTCCT GGCTAAGGTGCAGCGTCTGATCAAGGAGAGCTCGGAGCTCTCCTGTACCCTCGTGGCCTTGCGGAGCATC CTCAGGATCACAGCCAGTGACCGGCTCTTCACAGACATCTTCCGGGACTCGGGACTACTAGGTCTGCTGC TGGCCCAGCTGCGGAAACAGGCCAAGATCATGAGGAAGTCAGGAAACAAAGAGTGCAGCCCTGATGTGCA GGATCCTGAGAGGGAACTCACCTACGTGATGCTGAGCACTGTGGTCACACTTCTGCAAGGCTCTGTTCGA AATGCAGTTGTCCTAAAAGACCATGGCATGGTGCCCTTCATCAAGATCTTCCTGGATGATGAGTGCTACC GGGGCCCCTCGCTCAGCATCTTGGAGCAGCTCTCAGTCATCAATGCAGAGGAGTACATGAGCATCATTGT GGGAGCTTTGTGTTCGTCCACTCAGGGGGAGCTGCAGCTGAAGCTGGATCTTCTCAAGTCTCTGCTCCGG ATCCTGGAGACCCCCAAGGGCCATGCTGCATTCCGCGTCTCCAGTGGCTTCAATGGGCTGCTGTCCCTGC TTTCCGACCTGGAGGGGTCCCTCCAAGTACCTGAAGTGACAACATGTGGTGCAGTGTCCCCCAGCCAGAC CCTGGAGCTGGTGTTACACACCCTTTGTGTAGTGTCTGCTGCTCTGCACTTGGACCCTGTCAACGAGCAC TTCTTCAGGAGCAATGGACTCTTTGAGAAGCTGGCTGAGGACCTCTGTCTGCTGGGCTGTTTTGGGACCC CAGAGGAGGAGCGCACTCGATGGGACTCTTCATCTGACATGAAAGCCAGGCCCTTCATGGATTTGCTGAG CTGTGCCTTCTCCTCCAGCTGCCAGTTCCCACCCAGGCTGCAGAGCTGCTTACAGATTCTCAGCTTCCTA GAGAGCATGGCCAGTGGTACCCTCCACCTGCGGGGAGACCTGATGGAGCCAGCCAGGGCTGGGCAGGAGC CTTCTGTGGATGCTCAAAAGGCAGAGGCTGGTGGCCGCCAAGGCAAATTCAAGCAGTGGCCAGACATGGA GGACAGGATGGATGAAGGTGACGTCATGATCATGCATCCTGGGATCATTTGCATCATGGTGAGACTGCTG CCTCGTCTATACCTCGAAGATCACCCACAGCTTTCTGAGGAGATCCAGTGCTCTGTGGCCCGCCACCTTT TGTCCCTGGTGAAATCGGAGAAGAACCGCCAGGTCATGTGTGAGGCAGGGATGCTCCGGACACTCATGAC ATTTTGCCCCAGGACCCTCAGCACTGGTGGCAGTGACCTGCACTCGATTCTCATCAGGATCTTTGAAAAG CTTGGATCCCAAGCCATAGAGCCAGATGTGCTAAGCCCACGTCCTGGAGGGTCTCAGGCCCTCAGGCCCT CATGGGCCTCCCAATATTCGGCCACAGCCCTCCAGACAACACTGAGCCTCATCTCCATGACCTCCCCTCG AAATCTGCAGCCTCAGAGGGCGGCCCTGACACCATCATTTGTGGAATTTGACATGTCTTCAGAAGGTTAT GGCTGTCTGTTTACTCCGACCTTGTCTACAGTCATGGGGACCAGCACAGAGCACTCCATCTCTGGAGGGA CTGGATCAGGAGCTCCCAGGCCGTTCCCTCCGCCTGGAGGTCTCACCTTCTCCTGTTGGTTTCTGATCAG CCGGCAGGCCAATGTCATGGAGGGCCACCCACTGCGCTTCCTAACCCTGGTGCGCCACCTGGCCAGGACA GAGCAGCCCTTTGTCTGCTTCTCCATCAGCCTCTGCATGGATGACCTCTCCTTGGTTGTGTCCACAGAAG AGAAAGAGTTCCAGCCCCTGGATGCCATGGAGCCTGAGGATGAGGCAGAGCCCTCTGCAGGCCGCCAGCT CCAGGTCAGGTGCAGCCAGCTGCTGACATGTGGTCAGTGGTATCACCTGGCTGTGGTGGTCAGCAAAGAA ATGAAGAGGAATTGCAGTGTTACCACGTACCTGGATGGGCAAGCCATTGGTTCAGCCAAGATGTTGTATA TTCAGGCCTTACCGGGGTCTTTTTTTTCAATGGATCCCTCATCATTTGTGGATGTTTATGGCTATATTGG GACCCCCAGAGTTTGGAAACAGAAATCATCGCTAACCTGGCGTCTCGGGCCTGCATACCTCTTTGAAGAA GACATATCAGCGGATACTCTAGCACTTATTATCAAACTCGGCCCGAGGTACTGTGGTAACTTTCAAGCTG TGCACCTTCAAGGAGAAGACCCAGATGGCGAAGCCACGCCCCTCATCGCGGAGGAAAGGGTTTCCTTTGG TCTTTATGTACCCAGCTCTTCTATCACAAGCATAATGAACATCAGAAACACCTACAATGAGGTGGACAGC CGCTTGATCGCCAAGGAGATGAATATTTCCTCCCGTGACAATGCCACACCTGTGTTCTTGCTGAGAAACT GTGCCGGGCACCTCTCAGGGCCTCTGCGGACTCTCGGGGCTGTTGCTGTGGGCCAGTTAGGGGTAAGGGT GTTCCATTCCAGCCCTGCTGCCAGCAGCCTGGACTACATTGGAGGACCTGCCATCCTCCTAGGCCTCATC TCCCTGGCAACAGATGACCACACCATGTATGCAGCTATGAAGGTCCTGCACTCGGTCCTGACCAGCAATG CCATGTGTGATTACCTGATGCAGCACATCTGTGGGTACCAGATCTTAGCCTTTCTCCTGCGGAAGAAGAC ATCCTTTCTAAACCACCGCATTTTCCAGTTGATCCTCTCAGTGGCTGGCACAGCGGAGCTGGGTTTCAGG CCGTCTGCTGTCACTAACATGTGTATCTTCCAGCATGTGCTCTGCAATTTTGAGCTCTGGACAAACACTG CAGACAACCTGGAGCTCACGCTCTTTTCTCATCTCCTGGAGATCCTGCAGTCACCAAGGGAAGGACCCAG GAACGCTGAGGTAGCCCACCAGGCACAGCTGCTACCCAAGCTCTTGTTTCTCTTCAATGAGCCGAGTCTG GCTCTGTCCAAGGTCTCCACCATCATTGCCATTCTGGGCTGTCAGCTGAAGGGCCACTTCAACATCCAGG ACTTACTCAGGGTTGGGCTGTTTGTCATATACACCCTCAAGCCTTCATCAGTGAACGAGAGGCAGATCTG CCTGGATGGAGCCCAGGACCCCTCCAGACCCGCTGGAAGCCAAACATCTGGAAAGGCAATCTGGCTTAGA AACCAGTTGCTGGAGATGCTGTTTGGTGTAATATCTTCCTCCCAACTTCATCTGACCTCTGAGTTAAAGG AACAGGTGTTTCTGAGCCTGGGGCCCGACTGGTTCCTGCTCCTCCTGCAGGGTCACCTGCATCCCAGCAC CACCACGTTGGCATTGAAGCTGCTGCTGTACTTTCTGTCAAGCCCTCCCCTCCGTGGGCGATTTAGGGAT GGCCTATCTGCTGGATGCTGGGTAGAGAACTGCATGGACGGGGTGGACATTGTGATGGACAACCTGAAGA GCCGCCCTGCAGTGCCTGACCAGAGCCCCTGCCTGCTTCCTGGGTTCCGGGTCTTGAATGACTTTCTGGC CTACCATGTTCTCATTCCGGAAGTGTACCTCATCGTATCTTCCTTCTTCCTCCAGACACCACTTACAGAG CTGACCAATGGGCCCAGAGAGAACTTGGATTTGATGCTTCAGTGGCTTCTTCAGAAACATCATCAGCAAG AAGTCCTCCAGGCTGGGCTGTGCATAGAAGGTGCTCTGCTACTCCTGGGGATGCTGAAAGCCATCATGAA TCAGCCCCCTGCAGGTTCAGGAGACGGCGCCTGGGAGCAGACACTCCCCAGCAGTGTTCTTCAGTTCCTC CGCCTGGTGCACCGCTCCTACCCCCAGGACTCAGCGTGGAGGACCCCTGACTTCCTCCAGACTGTGGCCA TTATCACTTTCCCCCTGGAAACCCAGAAGGAGCCTCTGCCAAATGCAGAGGCTGGTGCTGTGCCTTCCCT TGCAAGCGTTTCCTACTTCACCCAGAAGCTGGTTGAGAAGATATATAGTGGGGTGTTCTCAGCGGATCCC AGGCACATCCTCCTCTTCATCACAGAGCACATCATAGCGGTCATCGAGAACCCTTCTTCCCAAAAGGACA CTGTCATGAGTGCATTGTACAGCAGTTTGAATAAAGTCATCCTGCATTGCCTCTCCAAGCCCCAGCAGTC CCTGTCTGAGTGCCTTGGCCTCCTCACCATCCTGGACTTCCTGCAGGAGCACTGGGACATCATCTTTGCC ACCTACAATTCCAATGTCAGCTTCCTCCTGTGCCTCATGCATTGTCTTTTGCTGCTGAATGCCAGGAGTT ATCCAGAAGGATTTGGATTGGAGCCCAAGCCCAGAATTACTCCTTATCACCAAGTCTTCCTCTCCCCAAA TGAAGAAGTGAAAGATAAGAAGGAGGAGGGCCTCCCAAGTCTGGGTGATGTTCAGCACAGCATCCAGAAG TCAGTGCGGGCGCTGTGGCAGCAGCTGGTGGCACAGAGGCGACAGACCCTGGAGGATGCCTTCAAGATAG ATCTCTCCGTGAAGGCTGGGGAAATCGAAGTGAAGATCGAAGAGATCACCCCACTTTGGGAGGAGACGAT GCTCAGAGCCTGGCAGCATTACTTAGCATCCGAGAAGAAGTCACTGGCCAGCCGTTCAAGTGTCATGCAC CACAGCAAAGTGACTTCATGGAGTGGGAGCCTATCCTCAGCCATGAGACTGATGCCAGGGCGACAGGCAA AGGATCCTGAGTGCAGAGCAGAGGATTTTGTGTCCTGCATCGAGAATTACAGAAGGAAAGGACAGGAGCT GTATGCATCTATATACAAGGACTATGTGCAGAGGAGGAAAAGTGGCAGCATCAAGGCAGCCACAGCCTGG GCCAGGATGCGGGAACAGCTCTTTGGGGAGCTGGGATTGTGGGGCCAAATGACAGAATCCACACGCTGCT CCCGGTGGGAACTGGATGGGAGGGAAGGACCGGCTCGCATGAGGAAACGCATCAGACACCTGTTAGCATG GGAGCCCCTGAACCTAGGATACAAGGAGAGCCAAGAAGGAAAGGGTGATGTTTCTCAGACAAATACTGGA AACCAAGATGAGCTGACAACAGAGGAAGCTGAGAGCAGGCCGGATGAGGTGGGAGTGGATTGCACACAAC TGACCTTCTTTCCTGCTTTGCATGAAAGCCTGCACTCAGAAGACTTCTTGGAATTGTGCCGGGAAAGACA AGTCATTTTACAAGAGCTTCTTGATGGAGAAAAGGTGTCACAGAAGGTCCCCATGGTGATTGTGCAGGGC CACCTGGTCTCAGAGGGGATCCTGCTCTTTGGCCAGCATCACTTCTACATCTGTGAGAACTTTACTCTGT CTCCTACAGGCGATGTCTACTGCACCCACCACTGCTTATCCAATATCAGTGACCCCTTCATTTTCAACAT GTGCAGCAAAGACCGGTCCTCTGACCATTACTCCTGCCAGCGCCATGCCTACAGTGACCTCAGAGAGCTC CGGCAGGCCCGCTTTCTCCTTCAGGATATTGCCCTGGAGATCTTCTTCCAAAACGGGTATTCCAAGCTCT TAGTCTTCTATAACAGCGACCGGAGTAAAGCCTTGAAAAGCTTTAGCACTTTTCAACCCAGCTTGAAAGG GAAAGGCACCACAGAGGACCCCTTCAACCTAAGGAAACACCCAGGCTTCGATAGGACCATGCTGCAGAGG TGGCAGAAAAGGGAGATCAGCAACTTTGAATACCTCATGTACCTCAACACACTGGCCGGAAGGACCTACA ATGACTACATGCAGTATCCCGTGTTTCCCTGGGTCCTCGCTGACTACACCTCAGAGATGTTGAACTTGAC GAATCCCAAGACTTTCCGGGATCTTTCTAAGCCAATGGGGGCTCAGACCAAGGAAAGGAAGTTGAAGTTT ACCCAGAGGTTTAAAGATGTTGAAAAGATTGAAGGAGACATGACCGTGCAGTGCCACTACTACACCCACT ATTCCTCAGCCATCATTGTCGCTTCCTACTTGGTCCGAATGCCACCATTCACGCAGGCCTTCTGCTCCTT ACAGGGCGGAAGCTTTGATGTGGCTGATAGAATGTTCCACAGTGTAAAGAGCACGTGGGAGTCTGCCTCC AAAGAGAACATGAGCGATGTCAGGGAGCTGACACCTGAATTCTTCTACCTGCCCGAGTTTTTAACCAACT GTAATGCAGTGGAGTTTGGCTGCATGCAGGATGGAACGACACTGGGGGATGTGCAGCTTCCTCCCTGGGC TGACGGGGATCCGAGGAAATTCATCAGCTTGCACAGACAGGCTCTGGAAAGTGACTTCGTCAGCAGCAAC CTCCACCACTGGATAGACCTAATTTTTGGGTATAAGCAGCAGGGGCCGGCTGCTGTAGAGGCAGTGAACA CTTTCCACCCCTACTTCTACGGTGATAGAATAGACCTGGGCAGCATCACTGACCCGCTGATCAAGAGCAC CATCCTGGGCTTCATCAGCAACTTTGGACAGGTGCCCAAGCAGATCTTCACTAAACCCCACCCATCCAGA AACACCACAGGGAAAAACCCAGGGCCTGGAAAGGATGCTTCCACCCCTGTAGGCCTCCCAGGCCACTCAC AGTCCTTCCTCCACAGCCTGCCAGCACTGAGACCCTCTCAGGTCACAGTCAAAGATATGTACCTTTTCTC TCTAGGGTCGGAATCCCCCAAAGGGGCCATCGGCCACATCGTCCCTACTGAGAAGTCAATCCTGGCAGTG GAGAAGAACAAGCTGCTGATGCCCCCTCTCTGGAACAGGACCTTCAGCTGGGGCTTTGATGACTTCAGTT GCTGCCTGGGGAGCTACGGCTCTGACAAGATCCTGATGACCTTTGAGAACCTGGCTGCCTGGGGTCCCTG TCTGTGCGCTGTATGCCCTTCCCCCACGATGATCGTCACATCCGGGGCCAGCGCAGTGGTGTGCATCTGG GAGCTGAGCCTGGTCAAAGGTCGCCCGAGAGGTCTGAAACTCCGACAGGCCTTGTATGGACACACTCAGG CGGTCACATGTCTGACAGCCTCTGTCACCTTCAGCCTCCTGGTGAGCGGATCCCAGGATCGCACTTGTAT CCTGTGGGACCTGGACCACCTCTCTCGTGTGGCCTGCCTGCCTGTCCACCGGGAAGGCATCTCAGCCATT GCCATCAGTGATGTCTCGGGAACCATTGTCTCCTGTGCCGGAGCCCACTTGTCCCTGTGGAATGTCAATG GACAGCCCCTGGCCAGTATTACCACAGCCTGGGGCCCAGAAGGAACCATAACGTGCTGCTGCATAGTAGA GGGGCCAGCGTGGGATGCAAGCCACGTGATCATCACGGGGAGTAAGGACGGAATGGTTCGGATTTGGAAG ACAGAGGACGTGAAGATGCCTGTTCCCAGGCAGGCAGTGATGGAGGAGCCCTCCACGGAGCCCCTAAGCC CCAGAGGTCACAAGTGGGCCAAGAATCTTGCCCTGAGCCGAGAGCTGGATGTCAGTGTTGCTCTGAGTGG CAAGCCCAGCAAGGCAAGTCCTGCTGTGACAGCTCTGGCCATCACTAGGAACCAGAGCAAGCTCTTGGTT GGCGATGAGAAGGGGCGAATCTTCTGCTGGTCTGCTGATGGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001146022
Insert Size	9075 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001146022.2, NP_001139494.1
RefSeq Size	9681 bp
RefSeq ORF	9075 bp
Locus ID	545030
Gene Summary	Plays a critical role in the regulation of cDC1-mediated cross-presentation of viral and tumor antigens in dendritic cells (PubMed:30409884). Mechanistically, acts near the plasma membrane and interacts with endosomal membranes to promote endosomal-to-cytosol antigen trafficking (PubMed:30409884). Plays also a role in B-cell survival through regulation of autophagy (PubMed:30257884).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.