Kalrn (NM_177357) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2210407G14Rik; AV235988; DUET; E530005C20Rik; Gm539; Hapip; TRAD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225438 representing NM_177357 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGACCGCTTCTGGGACCAGTGGTATCTTTGGTATCTCCGCTTGCTTCGGCTTCTGGATCGAGGAT CTTTTCGGAATGATGGTTTGAAAGCTTCTGATGTCCTTCCTATCCTAAAGGAGAAAGTGGCCTTTGTGTC TGGGGGGCGTGACAAGCGAGGTGGACCCATCCTGACCTTCCCTGCCCGAAGCAATCATGACAGAATAAGA CAGGAAGACCTACGGAAACTTGTGACGTATTTGGCCAGCGTGCCAAGTGAGGACGTTTGCAAACGTGGCT TCACCGTCATCATCGACATGCGGGGCTCCAAGTGGGATCTCATCAAGCCCCTCCTCAAGACGCTGCAGGA AGCCTTCCCCGCCGAGATCCATGTGGCCCTCATCATCAAGCCTGACAACTTCTGGCAGAAGCAGAAGACC AACTTTGGCAGCTCCAAATTCATCTTTGAGACAAGCATGGTGTCTGTGGAGGGCCTCACGAAGCTGGTGG ACCCCTCCCAGCTGACAGAGGAGTTTGACGGCTCCCTGGATTACAACCATGAGGAGTGGATTGAGTTGCG CCTCTCCCTGGAGGAGTTCTTCAACAGTGCGGTCCACCTGCTCTCACGCCTTGAGGACCTGCAGGAGATG CTGGCCCGGAAGGAGTTCCCTGTGGATGTGGAGGGCTCTCGGAGGCTTATCGACGAGCACACGCAACTTA AGAAAAAGGTGCTGAAGGCCCCCGTGGAAGAGCTGGACCGGGAGGGGCAGCGTCTACTGCAGTGCATCCG CTGCAGCGATGGCTTCTCAGGGCGCAACTGCATCCCAGGAAGTGCCGACTTCCAGAGCCTTGTACCCAAG ATCACCAGCCTCCTGGACAAGCTGCATTCCACCCGACAACACCTGCACCAAATGTGGCACGTGCGAAAGC TCAAGCTGGATCAGTGCTTCCAGCTGCGGCTCTTCGAGCAGGATGCTGAGAAGATGTTCGACTGGATAAG CCACAACAAGGAGTTATTTCTTCAGAGCCACACAGAGATCGGAGTCAGCTACCAGCATGCACTAGACCTC CAGACACAGCACAATCACTTTGCCATGAACTCCATGAATGCCTATGTCAACATCAACCGCATCATGTCTG TCGCTTCCCGCCTCTCAGAGGCCGGCCATTATGCCTCCCAGCAAATCAAGCAGATCTCCACGCAGCTAGA CCAGGAGTGGAAGAGCTTTGCCGCTGCCCTGGATGAACGCAGTACAATCCTGGCCATGTCTGCTGTGTTC CACCAGAAGGCAGAGCAGTTCCTGTCAGGCGTGGATGCCTGGTGTAAGATGTGCAGCGAAGGCGGCCTTC CTTCTGAGATGCAGGACCTGGAGCTGGCAATCCACCATCACCAGTCCTTATATGAGCAGGTGACCCAGGC CTACACAGAGGTCAGCCAGGATGGCAAAGCACTACTGGATGTCCTGCAGCGTCCCCTGAGTCCTGGGAAC TCTGAGTCCCTCACAGCCACAGCCAACTACTCCAAGGCGGTCCACCAGGTGCTGGATGTGGTCCACGAGG TGCTGCACCATCAGCGACGGCTCGAGAGCATCTGGCAGCACCGCAAGGTGCGGCTTCACCAGCGGCTGCA GCTGTGTGTCTTCCAGCAGGATGTGCAGCAGGTGCTGGACTGGATTGAAAACCATGGCGAGGCCTTTCTC AGCAAACACACTGGGGTTGGCAAGTCCTTGCATCGAGCACGTGCACTACAGAAGAGGCATGATGACTTTG AAGAAGTGGCACAGAATACATACACCAATGCGGACAAGCTCCTAGAAGCAGCGGAGCAGCTGGCTCAGAC TGGGGAATGTGACCCGGAGGAGATCTACAAGGCAGCCCGGCACCTGGAGGTGCGCATCCAAGACTTTGTG CGCAGAGTGGAGCAGCGGAAGCTGCTTCTGGACATGTCGGTCTCTTTCCACACGCACACCAAAGAGCTGT GGACATGGATGGAGGACCTTCAGAAGGAGGTGCTGGAGGATGTCTGTGCAGACTCTGTGGATGCGGTCCA GGAGCTGATCAAGCAGTTCCAGCAGCAGCAGACCGCCACCCTGGATGCCACTCTCAATGTCATCAAGGAA GGCGAAGACCTTATCCAGCAGCTCAGGTCAGCGCCTCCCTCCCTGGGGGAGCCCACCGAGGCCAGGGACT CAGCGATGTCCAACAACAAGACACCCCACAGTAGCTCTATCAGCCATATTGAGTCTGTCCTCCAGCAGCT GGACGATGCTCAGGTACAGATGGAAGAGCTGTTTCACGAGCGGAAGATCAAGCTGGACATCTTCCTACAG CTGCGCATCTTTGAACAGTACACCATTGAGGTCACTGCAGAGCTGGATGCCTGGAACGAGGACTTGCTCC GGCAGATGAATGATTTCAACACAGAGGATCTGACCCTGGCTGAGCAGCGGCTACAGCGCCACACAGAACG GAAGCTTGCCATGAACAACATGACGTTCGAGGTCATCCAGCAGGGCCAGGACCTGCACCAGTACATCATG GAAGTGCAAGCGTCAGGAATCGAGTTAATCTGTGAGAAGGACCTTGACCTGGCAGCTCAGGTGCAGGAGC TGCTGGAGTTTCTCCATGAGAAGCAGCACGAGCTGGAGCTCAATGCGGAGCAGACGCACAAGCGGCTAGA GCAGTGTCTCCAGCTGAGGCACCTCCAGGCTGAGGTCAAACAGGTTCTGGGATGGATCCGCAATGGAGAG TCGATGCTCAACGCCAGCCTGGTCAACGCCAGCTCTTTGTCGGAGGCAGAGCAGCTGCAGCGGGAGCATG AACAGTTCCAGCTAGCCATCGAGAAGACGCACCAGAGCGCCCTGCAGGTACAGCAGAAGGCAGAGGCACT GCTCCAGGCCGGCCACTACGACGCAGACGCCATCCGGGAATGTGCTGAGAAGGTGGCCCTCCACTGGCAA CAGCTCATGCTAAAGATGGAGGACCGGCTGAAGCTTGTCAACGCCTCTGTGGCCTTTTACAAGACCTCTG AGCAGGTGTGCAGTGTTCTGGAGAGCTTGGAGCAGGAGTACCGGAGAGACGAGGACTGGTGTGGTGGGAG AGACAAGCTGGGGCCGGCGGCAGAGATGGACCACGTTATTCCACTCCTCAGCAAGCACCTGGAGCAGAAG GAGGCCTTCCTGAAGGCCTGCACGCTGGCAAGGCGGAACGCCGAGGTGTTCCTCAAATACATCCACAGGA ACAATGTGAGCATGCCTAGTGTTGCCAGCCACACCCGGGGGCCTGAACAGCAAGTAAAAGCCATCTTGAG CGAGCTCTTGCAGAGGGAGAATCGAGTCCTGCACTTCTGGACCTTGAAGAAGCGGCGGTTAGACCAGTGT CAGCAATATGTGGTGTTTGAGCGCAGTGCTAAGCAGGCACTGGACTGGATCCAAGAAACAGGAGAATATT ACCTCTCAACACATACCTCCACCGGAGAGACCACGGAGGAGACTCAGGAGCTGCTGAAAGAGTATGGAGA ATTCAGGGTGCCTGCCAAGCAAACGAAGGAGAAGGTGAAACTTCTGATCCAGCTAGCAGACAGCTTTGTG GAAAAGGGCCACATCCACGCCACAGAGATACGGAAATGGGTGACCACGGTGGACAAGCACTACCGCGATT TCTCCCTGAGGATGGGGAAGTACCGGTACTCGCTGGAGAAGGCGCTGGGGGTCAACACGGAGGATAACAA GGACCTAGAGCTGGACATCATCCCAGCAAGCCTTTCGGATCGGGAGGTCAAGCTCCGGGACGCTAACCAT GAGATCAATGAAGAGAAACGGAAGTCAGCTCGAAAGAAAGAGTTCATTATGGCTGAACTGCTCCAAACTG AGAAGGCTTATGTAAGGGACTTGCACGAGTGCTTGGAGACCTACCTGTGGGAGATGACTAGTGGTGTGGA AGAGATACCCCCCGGCATCCTCAATAAGGAACATATCATCTTCGGCAACATCCAGGAGATCTATGACTTC CATAACAACATCTTCCTCAAAGAGCTGGAGAAGTACGAGCAGCTGCCTGAGGATGTGGGACACTGCTTTG TTACCTGGGCAGACAAATTTCAGATGTATGTCACCTACTGTAAAAACAAGCCTGATTCCAACCAGCTGAT CCTGGAGCATGCGGGCACCTTCTTTGATGAGATCCAGCAGCGGCATGGCCTGGCCAACTCCATCTCCTCC TACCTAATTAAGCCTGTCCAGAGGGTTACCAAGTACCAGCTGCTCCTGAAGGAACTTCTAACTTGCTGTG AAGAAGGGAAAGGAGAGCTGAAGGATGGCCTGGAGGTGATGCTCAGTGTCCCCAAGAAAGCCAACGATGC CATGCATGTCAGCATGCTGGAAGGGTTCGATGAAAACTTGGATGTACAGGGGGAGTTGATTCTCCAGGAT GCCTTTCAAGTGTGGGACCCAAAGTCGCTGATCCGGAAGGGGCGGGAGCGCCATCTGTTCCTCTTTGAGA TCTCCTTGGTGTTTAGCAAAGAGATCAAAGACTCTTCAGGACACACGAAATATGTTTACAAGAACAAGCT ACTGACCTCAGAGCTGGGTGTGACCGAGCATGTAGAAGGTGACCCCTGCAAATTCGCCTTGTGGTCTGGG CGCACCCCATCCTCAGACAATAAAACAGTGCTCAAAGCTTCCAACATCGAAACCAAGCAGGAATGGATCA AGAACATTCGAGAAGTGATTCAGGAGAGGATCATTCATCTTAAAGGCGCTTTAAAGGAGCCAATTCAGCT CCCCAAAACACCAGCCAAACTGAGGAACAATAGTAAGAGGGATGGGGTAGAGGATGGTGACAGCCAAGGG GATGGGAGCAGCCAGCCAGATACCATATCCATTGCCTCCAGGACCTCTCAGAACACAGTGGAAAGTGACA AGCTCTCAGGTGGATGTGAGCTGACCGTGGTCCTCCAGGACTTCAGCGCAGGCCACAGCAGCGAGCTGAG CATCCAGGTGGGCCAGACGGTGGAGCTGCTGGAACGACCGAGTGAGAGGCCTGGCTGGTGTCTGGTCCGC ACCACAGAGCGGAGCCCCCCACAGGAAGGCCTGGTACCCAGCAGCGCCCTGTGCATCTCACACTCTCGCA GCAGCGTGGAAATGGACTGCTTCTTCCCCTTGAAAGATTCATACTCTCATTCCTCCAGTGAGAATGGAGG CAAATCCGAGTCCGTGGCCCACCTGCAGTCTCAGCCCTCCCTGAACTCCATCCACAGTTCCCCTGGTCCC AAGCGCTCCACCAACACCCTCAAAAAGTGGCTGACCAGCCCCGTGCGCCGCCTCAACAGCGGAAAAGCCG ACGGGAACATCAAAAAGCAGAAGAAAGTCCGGGATGGGCGGAAGAGCTTCGATCTGGGATCGCCCAAGCC TGGGGATGAGACGACACCTCAGGGAGACAGCGCTGACGAGAAGAGCAAGAAGGGTTGGGGCGAAGACGAG CCAGATGAAGAGTCACACACACCCCTCCCTCCGCCCATGAAGATCTTTGACAACGACCCCACACAGGATG AGATGTCGTCTTTGCTAGCAGCCCGGCAGGCTCCCCCTGACGTGCCTACTGCTGCAGACCTTGTCAGTGC AATAGAAAAGTTGGTCAAAAATAAGCTGACTCTGGAAGGAGGCTCTTACCGGGGGAGCTTGAAGGACCCT ACAGGCTGCCTGAATGAGGGGATGACCCCTCCCACACCTCCTAGAAACCTGGAAGAAGAACAGAAAGCCA AGGCGCTGAGAGGCAGGATGTTTGTCCTGAATGAGCTGGTACAGACAGAGAAGGACTACGTCAAGGACCT GGGGATTGTGGTGGAGGGCTTCATGAAGAGAATAGAAGAGAAGGGGGTCCCTGAGGACATGCGAGGGAAG GAAAAGATCGTGTTTGGAAATATCCACCAGATTTACGACTGGCATAAAGATTTTTTCCTGGCTGAACTGG AAAAATGTATCCAAGAGCAAGACAGATTGGCCCAGCTCTTCATTAAACACGAGCGGAAGCTGCACATCTA CGTGTGGTACTGCCAGAACAAGCCGCGTTCCGAGTACATCGTGGCTGAGTACGATGCCTATTTTGAGGAG GTTAAACAAGAGATCAATCAAAGGCTGACCCTTAGTGACTTCCTGATCAAGCCCATTCAGAGAATAACAA AGTACCAGCTGCTACTCAAGGACTTCCTGCGATACAGCGAGAAGGCTGGTTTGGAGTGTTCCGATATTGA GAAAGCAGTGGAGTTAATGTGCCTTGTCCCCAAGCGCTGTAATGACATGATGAATCTGGGACGCCTGCAG GGCTTCGAGGGCACCCTGACCGCCCAGGGCAAGCTACTGCAGCAGGACACATTCTATGTGATCGAGCTGG ATGCCGGCATGCAGTCCCGGACCAAGGAGAGGCGTGTGTTTCTCTTTGAGCAGATTGTCATCTTCAGTGA ACTGCTCCGAAAGGGGTCCCTCACCCCAGGCTACATGTTCAAAAGAAGCATCAAGATGAATTACTTGGTC CTGGAAGACAATGTGGATGGTGACCCCTGCAAATTTGCACTCATGAACAGGGAGACTTCAGAAAGGGTCA TTCTACAGGCTGCCAACTCAGACATCCAGCAGGCCTGGGTGCAGGACATCAACCAAGTCCTAGAAACACA GAGGGACTTCTTAAATGCACTACAGTCACCCATTGAGTATCAGCGGAAGGAGAGAAGCACAGCTGTGATC CGGTCCCAGCCCCCGAGGGTTCCACAAGCCAGCCCCAGGCCCTACTCTTCTGGCCCGGTGGGCTCAGAGA AGCCCCCCAAGGGCTCCAGCTATAACCCGCCTCTGCCACCCCTGAAGATATCTACCTCTAATGGCAGTCC AGGGTTCGACTACCATCAGCCTGGGGACAAGTTTGACGCTAGCAAGCAGAACGACCTGGGAGGCTGCAAT GGGACATCAACCATGACTGTAATCAAAGATTACTATGCACTGAAGGAGAATGAAATCTGCGTGAGCCAAG GTGAGGTGGTACAGGTCCTCGCCGTCAACCAGCAGAACATGTGTCTGGTGTACCAGCCTGCCAGCGACCA TTCCCCGGCAGCAGAGGGCTGGGTCCCAGGCAGCATCCTGGCGCCCCTCGCCAAAGCCACAGCAGCGGCC GAAAGTAGTGACGGGAGCATCAAGAAGTCATGCTCTTGGCACACTCTACGCATGAGAAAGCGGGCAGACG TGGAGAACTCGGGTAAAAATGAAGCCACAGGGCCTCGTAAACCCAAGGATATTCTGGGCAACAAAGTCTC TGTTAAAGAGACGAACAGTTCCGAGGAGTCAGAGTGTGATGATCTTGACCCTAATACTAGTATGGAGATC TTGAATCCAAATTTCATCCAGGAAGTGGCTCCAGAATTCCTCGTGCCCTTAGTAGATGTGACCTGTTTGC TTGGAGACACAGTGCTACTGCAGTGTAAAGCCTGTGGGCGGCCAAAGCCCAGCATCACCTGGAAGGGTCC GGATCAGAACATCCTAGACACTGACAACAGTTCAGCAACATACACCATCTCCTCCTGTGATTCTGGGGAA AGCACCCTCAAGATCTGCAACCTGATGCCCCAGGATAGTGGGATTTATACCTGCATAGCAGCAAACGACC ACGGGACTGCATCCACATCTGCTACAGTTAAGGTGCAAGGTGTCCCCGCAGCCCCCAACCGCCCAATCGC ACAGGAGAGGAGCTGCACCTCCGTCATCCTCCGCTGGCTGCCCCCAGCCAGCACCGGAAACTGCACTATT TCGGGGTACACAGTGGAGTATCGCGAGGAAGGTTCCCAGGTCTGGCAGCAATCCGTAGCTTCGACCTTGG ACACTTACCTGGTCATTGAAGACCTTAGTCCTGGGTGCCCTTATCAGTTCAGGGTCAGTGCCAGCAACCC CTGGGGAATCAGCCTTCCCAGTGAGCCCTCGGAGTTTGTGCGCCTTCCAGAGTACGATGCAGCCGCCGAC GGTGCCACCATTTCTTGGAAGGAAAATTTTGATTCTGCTTACACTGAACTGAACGAGATTGGCAGAGGCC GTTTCTCTATTGTAAAGAAATGCATTCACAAAGCTACCCGCAAAGATGTTGCTGTGAAATTTGTGAGCAA AAAAATGAAGAAGAAGGAGCAGGCTGCTCACGAGGCCGCCCTGCTCCAGCACCTGCAGCACCCTCAGTAT GTCACGCTCCATGACACCTACGAGTCCCCCACATCCTACATCCTGATTTTGGAACTGATGGATGACGGCC GACTCTTGGACTACCTTATGAATCATGATGAGCTGATGGAAGAAAAGGTAGCTTTCTACATTCGAGACAT CATGGAAGCTCTGCAGTACCTGCACAACTGTAGGGTCGCACATTTGGACATAAAGCCCGAAAACCTGCTC ATTGACCTCCGGATCCCAGTGCCTCGAGTGAAGCTCATCGACTTGGAAGACGCTGTCCAGATCTCAGGCC ACTTCCATATCCACCATCTGCTGGGGAACCCTGAGTTCGCTGCCCCCGAAGTGATCCAAGGCATCCCTGT CTCCCTGGGCACAGACATCTGGAGCATCGGGGTTCTGACATATGTCATGCTTAGTGGGGTCTCCCCCTTC TTAGATGAGAGCAAAGAGGAAACCTGTATCAACGTGTGCAGGGTGGACTTTAGCTTCCCCCACGAGTACT TCTGCGGTGTGAGCAATGCTGCCAGGGATTTCATCAACGTAATCTTACAGGAAGACTTCCGGAGGCGGCC CACAGCAGCCACGTGCCTGCAGCATCCCTGGCTACAGCCCCACAACGGCAGCTACTCCAAGATCCCCTTG GACACCTCCCGTCTGGCCTGCTTCATAGAACGCCGCAAGCACCAGAATGATGTGCGGCCCATTCCCAATG TCAAGAGTTACATCGTCAATAGGGTGAACCAAGGGACCTCGCTATCCCACAACCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177357
Insert Size	8949 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177357.3, NP_796331.2
RefSeq Size	15439 bp
RefSeq ORF	8949 bp
Locus ID	545156
Gene Summary	Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on transcript alignments and orthologous data, including rat AF232669.1.

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