Nbea (NM_030595) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Lyst2; mKIAA1544
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225434 representing NM_030595 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCGACAAGCCGGGCCCGGGGCTCGAGCCGCAGCCCGTCGCGCTCCTGGCCGTCGGGGCCGGCG GCGGTGCAGGCGGCGGAGGCGCCATGGGGGAGCCTCGGGGGGCGGCCGGCTCCGGCCCGGTGGTGCTGCC GGCGGGCATGATCAACCCTTCGGTGCCGATCCGCAACATCCGTATGAAATTCGCCGTTCTGATCGGACTC ATACAGGTCGGCGAGGTCAGCAACAGGGACATCGTGGAGACCGTGCTCAACCTGCTGGTTGGTGGGGAAT TTGATTTGGAGATGAACTTTATTATCCAGGATGCTGAGAGCATAACATGTATGACAGAGCTTTTGGAGCA CTGTGATGTGACATGCCAAGCAGAAATATGGAGCATGTTTACAGCCATCCTACGGAAGAGTGTTCGGAAT TTACAGACCAGCACAGAAGTGGGGCTAATTGAGCAAGTGTTGCTGAAAATGAGTGCAGTAGATGACATGA TAGCAGATCTTCTAGTGGATATGCTGGGAGTTCTGGCAAGCTACAGCATTACTGTCAAGGAGCTAAAGCT TTTATTCAGCATGCTTCGAGGAGAAAGTGGAATCTGGCCAAGGCATGCAGTAAAATTATTATCAGTTCTT AATCAGATGCCACAAAGACATGGTCCTGATACATTTTTCAATTTTCCTGGGTGTAGTGCTGCGGCAATTG CATTGCCTCCCATTGCAAAGTGGCCTTATCAGAATGGCTTCACTCTCAACACTTGGTTTCGAATGGATCC ATTAAATAACATTAATGTTGATAAGGATAAACCTTATCTGTATTGTTTTCGTACTAGCAAAGGAGTGGGC TACTCTGCTCATTTTGTTGGCAACTGTTTGATAGTCACATCTCTGAAGTCCAAAGGAAAAGGTTTCCAGC ACTGTGTGAAGTATGATTTCCAGCCTCGCAAGTGGTACATGATCAGCATCGTCCACATCTACAATCGATG GAGGAACAGTGAGATCCGTTGTTATGTTAATGGCCAGCTGGTATCCTATGGTGACATGGCTTGGCATGTT AACACAAATGATAGCTATGACAAGTGCTTTCTTGGATCTTCAGAAACTGCTGATGCCAATAGGGTGTTCT GTGGCCAGCTTGGGGCTGTGTATGTGTTCAGTGAAGCTCTTAACCCAGCACAGATATTTGCAGTTCATCA ATTAGGACCTGGTTATAAGAGTACCTTCAAGTTTAAGTCTGAGAGTGATATTCATTTGGCAGAACATCAC AAACAGGTTCTGTATGATGGGAAGCTTGCCAGTAGTATTGCCTTTTCCTACAACGCCAAGGCCACCGATG CTCAGCTTTGTCTGGAGTCCTCACCAAAAGAAAATGCATCCATCTTTGTGCACTCCCCACATGCACTGAT GCTCCAGGATGTGAAAGCTATAGTAACACATTCAATTCATAGTGCAATCCATTCAATTGGAGGGATTCAA GTACTTTTCCCACTTTTTGCCCAGCTGGACAATCGGCAGCTTAATGACAGTCAAGTAGAAACAACTGTCT GTGCTACTCTTTTGGCATTCCTGGTTGAACTACTTAAAAGTTCAGTAGCCATGCAAGAACAGATGCTGGG CGGAAAAGGCTTTTTAGTCATTGGCTACTTACTTGAAAAGTCATCAAGAGTTCATATAACTAGAGCTGTC TTGGAGCAGTTTTTATCATTTGCAAAATACCTCGATGGCTTATCTCATGGAGCCCCATTGCTGAAGCAGC TTTGTGACCACATTTTGTTCAATCCCGCCATCTGGATACACACACCTGCAAAGGTTCAACTTTCCCTATA TACTTACTTGTCTGCTGAGTTCATTGGAACTGCTACCATCTATACCACCATACGCAGAGTTGGGACAGTA TTGCAGCTCATGCACACATTAAAATATTACTACTGGGTTATTAACCCTGCTGACAGCAGTGGCATTGCAC CTAAAGGACTAGATGGTCCCCGGCCATCACAAAAAGAAATCATATCACTTCGGGCTTTTATGCTGCTTTT TCTGAAACAGCTCATATTAAAGGATCGAGGTGTGAAAGAAGATGAACTTCAGAGTATATTAAATTATTTA CTTACAATGCATGAGGATGAAAATATTCATGATGTCTTACAGTTGTTAGTGGCTCTAATGTCAGAACACC CAGCCTCAATGATACCAGCATTTGATCAAAGAAATGGAATAAGGGTGATCTACAAATTATTGGCTTCTAA AAGTGAAAGTATCTGGGTCCAAGCGCTGAAAGTTCTGGGATATTTTTTGAAGCATTTAGGTCATAAGCGA AAGGTTGAAATTATGCATACCCATAGTCTCTTCACTCTTCTTGGAGAAAGGCTGATGCTGCATACAAACA CTGTGACTGTCACTACATACAACACACTTTATGAGATCTTGACAGAGCAAGTCTGTACTCAAGTTGTGCA TAAACCACACCCGGAGCCAGACTCCACAGTCAAAATCCAGAATCCAATGATTCTTAAGGTGGTGGCAACT TTGTTAAAGAACTCAACACCAAGTGCAGAATTGATGGAAGTCCGTCGGCTGTTTTTATCCGACATGATTA AGCTTTTCAGTAACAGCCGTGAAAATAGAAGATGCTTACTGCAATGTTCAGTGTGGCAGGACTGGATGTT TTCTCTTGGCTATATCAATCCAAAAAGTTCTGAGGAACAGAAGATTACTGAGATGGTCTACAACATTTTC CGGATTCTTTTGTATCATGCAATAAAATATGAATGGGGAGGCTGGAGAGTCTGGGTGGACACACTCTCAA TAGCCCATTCCAAGGTTACTTACGAAGCACACAAAGAGTACCTGGCCAAAATGTATGAGGAGTACCAGCG ACAAGAGGAGGAGAACATTAAGAAAGGAAAGAAAGGAAATGTGAGCACCATCTCTGGTCTCTCATCACAG ACAGCAGGAGCAAAAGGAGGAATGGAAATCCGGGAGATAGAAGACCTTTCCCAAAGCCAGAGCCCAGAGA GTGAGACAGACTACCCAGTCAGCACAGATACACGAGACTTGCTCATGTCAACCAAAGTATCAGATGATAT TCTTGGAAGTTCAGATAGGCCTGGCAGTGGAGTGCACGTAGAAGTGCATGATCTTCTAGTTGACATAAAA GCAGAGAAAGTGGAAGCCACAGAAGTAAAGCTTGATGATATGGACTTGTCACCAGAGACATTGGTGGGTG GAGAGAATGGTGCCCTTGTGGAAGTGGAGTCCTTGTTGGATAATGTGTATAGTGCTGCTGTAGAGAAACT CCAGAACAATGTGCATGGAAGTGTGGGTATCATTAAAAAAAACGAAGAGAAAGACAACGGCCCGTTGATC ACGCTAGCAGATGAGAAGGAGGAGCTTCCAAACAGTAGCACACCCTTCCTCTTTGATAAAATACCCAGAC AGGAAGAGAAATTACTTCCAGAACTTTCTAGCAATCACATCATCCCAAATATTCAGGATACCCAAGTACA TCTTGGTGTCAGTGATGATCTTGGGTTGCTTGCTCACATGACTGCGAGTGTAGAGTTAACGTGCACATCA AGCATAATGGAAGAGAAAGACTTCAGAATTCATACGACGTCAGATGGAGTGAGCAGTGTCTCTGAACGAG AGTTAGCATCGTCAACTAAGGGGTTAGACTATGCGGAAATGACTGCCACAACTTTGGAAACAGAGTCTTC CAATAGCAAAGCTGTACCAAATGTTGATGCAGGAAGTATAATTTCAGACACTGAGCGATCTGATGATGGC AAAGAATCGGGAAAGGAAATCCGAAAAATCCAGACCACTGCTACAACACAAGCTGTCCAGGGCCGGTCTT CCACCCAGCAAGACCGAGATCTCAGAGTCGATTTAGGATTCCGAGGGATGCCAATGACTGAGGAACAGCG GCGCCAGTTCAGCCCTGGCCCCCGGACCACCATGTTTCGGATCCCTGAGTTTAAGTGGTCTCCGATGCAC CAGAGACTGCTCACTGATCTGTTGTTTGCATTAGAGACAGATGTGCATGTGTGGAGAAGCCATTCTACAA AGTCTGTAATGGATTTTGTCAACAGCAATGAAAACATTATATTTGTACATAACACAATTCACCTCATTTC CCAAATGGTAGACAACATCATCATTGCCTGTGGAGGAATTTTACCTTTGCTCTCAGCAGCTACATCGCCA ACTGGTTCTAAGACTGAATTGGAAAACATTGAAGTGACACAAGGCATGTCAGCAGAGACAGCAGTGACGT TTCTCAGCCGACTCATGGCAATGGTTGATGTCCTTGTGTTTGCCAGCTCTCTAAATTTTAGTGAGATTGA AGCTGAAAAAAACATGTCCTCTGGAGGGCTAATGAGACAGTGCCTGCGGCTAGTTTGTTGTGTAGCTGTG AGAAACTGTTTAGAATGCCGACAGAGACAGAGAGACAGGGGAAGCAAATCTTCCCACGGAAGCAGCAAAC CTCAGGAAGCTCCCCACAGTGTGACTGCCGCATCCGCATCCAAGACTCCTTTAGAAAACGTTCCAGGTAA CCTTTCTCCCATCAAGGATCCCGATAGGCTTCTTCAGGATGTTGATATCAATCGCCTTCGTGCTGTTGTC TTTCGAGATGTGGATGATAGCAAGCAGGCACAATTCTTAGCCCTGGCTGTTGTTTACTTTATTTCTGTTC TGATGGTATCGAAGTATCGGGACATATTAGAACCCCAGAGAGAGACTGCAAGAACTGGAAGCCAACCAGG TAGAAATATCAGACAAGAAATAAATTCACCAACTAGTACAGTTGTGGTCATACCATCTATCCCTCATCCA AGTTTGAACCATGGACTCCTTGCCAAGCTGATGCCTGAGCAGAGTTTTGCCCACTCATTTTATAAAGAAA CACCTGCTACATTTCCAGACACTGTAAAGGAGAAGGAAACACCAACCCCTGGTGAAGATATTCAGCTAGA AAGTTCAGTTCCTCACACAGATTCCGGAATGGGAGAGGAGCAAGTAGCTAGCATCCTGGATGGGGCAGAG CTGGAGCCTGCTGCAGGTCCTGATGCAATGAGTGAACTTTTATCCACTTTGTCATCAGAAGTAAAGAAAT CACAGGAGAGCTTAACTGAACATCCCAGTGAAATGCTGAAGCCTGCACCGTCCATATCTAGCATTAGTCA AACCAAAGGTATTAATGTGAAGGAGATACTAAAAAGTCTTGTGGCTGCCCCAGTTGAGATTGCTGAGTGT GGCCCTGAGCCTATTCCATACCCAGATCCGGCACTGAAGAGAGAAGCACACGCTATTCTTCCTATGCAGT TCCATTCCTTTGACAGGAGTGTGGTGGTACCTGTAAAGAAGCCGCCTCCAGGTAGTCTAGCTGTCACCAC GGTGGGAGCCACTGCTGCAGGAAGTGGGCTGCCAACAGGCAGCACCTCAAGTATATTTGCTGCCCCTGGA GCTACACCAAAAAGTATGATTAACACGACAGGTGCTGTGGATTCAGGGTCCTCCTCCTCTTCCTCCTCTT CTAGTTTTGTTAATGGTGCTACCAGCAAAAATCTTCCAGCTGTACAAACCGTAGCCCCAATGCCAGAAGA TTCAGCTGAGAACATGAGCATCACTGCAAAACTTGAAAGAGCTTTAGAAAAAGTTGCTCCTCTTCTTCGT GAAATTTTTGTAGACTTTGCCCCATTCCTATCTCGTACACTTCTGGGCAGTCATGGACAAGAGCTATTGA TAGAAGGCCTTGTTTGTATGAAGTCCAGCACATCCGTGGTGGAGCTAGTCATGCTGCTTTGCTCTCAGGA ATGGCAGAACTCTATCCAGAAGAATGCAGGCCTTGCATTTATTGAACTCATCAATGAAGGAAGGTTGTTG TGCCATGCTATGAAGGACCACATAGTCCGTGTTGCAAATGAAGCTGAGTTTATTTTGAACAGACAGAGAG CAGAGGATGTACATAAGCATGCAGAGTTTGAGTCGCAGTGTGCACAGTATGCTGCTGACAGGAGAGAGGA GGAGAAGATGTGTGACCACCTCATCAGTGCTGCCAAGCACCGGGACCACGTGACAGCCAACCAGCTGAAA CAGAAGATCCTCAACATCCTCACCAACAAGCATGGCGCCTGGGGGGCCGTGTCACACAGTCAATTGCATG ACTTCTGGCGCCTGGATTACTGGGAGGATGATCTTCGTCGAAGGAGAAGATTTGTTCGAAATGCCTTTGG CTCTACACACGCTGAAGCACTGCTCAAATCTGCAGTAGAGTATGGCACTGAAGAAGATGTGGTGAAGTCC AAGAAAGCTTTCAGAAGCCAAGCGATTGTGAACCAGAACTCAGAGACTGAGCTGATGCTGGAAGGAGATG ATGATGCCGTCAGCCTGCTCCAGGAGAAAGAAATTGACAACCTTGCAGGCCCTGTGGTTCTCAGCACCCC TGCCCAGCTCATTGCTCCTGTGGTGGTGGCCAAAGGAACCCTCTCCATCACTACAACAGAAATCTACTTC GAGGTAGATGAGGATGATGCTGCCTTCAAGAAGATCGACACAAAAGTTCTTGCATACACTGAGGGACTTC ATGGGAAATGGATGTTCAGTGAGATTCGAGCTGTCTTTTCCAGACGGTACCTTCTGCAGAACACTGCGCT GGAAGTGTTCATGGCGAACCGAACCTCTGTGATGTTTAATTTTCCTGACCAAGCAACAGTTAAAAAAGTT GTCTACAGCTTGCCTCGGGTTGGAGTGGGGACCAGCTATGGTTTGCCACAAGCCAGGAGGATATCACTGG CCACTCCTCGACAGCTGTATAAGTCTTCCAATATGACTCAGCGCTGGCAAAGAAGGGAAATCTCCAACTT TGAGTATTTGATGTTTCTCAACACGATAGCAGGTCGGACGTATAATGATCTGAACCAGTATCCTGTGTTT CCATGGGTGTTAACAAACTATGAATCAGAGGAGTTGGACCTGACTCTCCCAGGAAACTTCAGGGATCTGT CAAAGCCAATTGGTGCTTTGAACCCGAAGAGAGCAGTGTTTTACGCAGAGCGCTATGAGACATGGGAGGA GGATCAAAGCCCACCCTTCCACTACAACACACATTACTCAACGGCGACTTCCGCCCTGTCATGGCTTGTT CGGATTGAGCCATTCACAACCTTCTTCCTCAATGCAAATGATGGGAAATTTGACCATCCAGACCGAACCT TCTCATCCATTGCAAGGTCATGGAGAACCAGTCAGAGAGATACATCCGATGTCAAGGAACTAATTCCAGA GTTCTACTACCTACCAGAGATGTTTGTCAACAGCAATGGGTACCATCTTGGAGTGAGGGAGGACGAAGTG GTGGTTAATGATGTGGACCTGCCCCCCTGGGCCAAGAAGCCAGAAGACTTTGTGCGGATCAACAGGATGG CCCTGGAAAGTGAATTTGTTTCTTGCCAACTCCATCAATGGATTGACCTTATATTTGGCTACAAACAGCG AGGGCCAGAGGCAGTCCGTGCTCTCAATGTTTTCCACTACTTGACCTACGAAGGCTCTGTAAACCTGGAC AGCATCACAGACCCTGTGCTCCGGGAGGCCATGGAAGCACAGATACAGAACTTTGGACAGACGCCATCTC AGTTGCTCATTGAGCCGCATCCGCCTAGGAGTTCAGCCATGCATCTGTGTTTCCTTCCACAGAGCCCACT CATGTTCAAAGATCAGATGCAGCAGGATGTGATCATGGTGCTGAAGTTTCCATCCAATTCTCCTGTGACT CATGTGGCTGCCAACACCCTGCCCCACCTGACCATCCCTGCAGTGGTGACAGTGACCTGCAGCCGACTGT TTGCAGTGAACAGATGGCACAACACAGTCGGCCTCAGAGGAGCCCCCGGATACTCCTTGGATCAAGCACA CCATCTTCCCATTGAGATGGACCCATTAATCGCAAATAACTCTGGTGTGAACAAGCGGCAGATCACAGAC CTTGTAGACCAGAGCATCCAGATCAATGCCCACTGCTTCGTGGTCACAGCTGATAATCGCTACATCCTCA TCTGTGGGTTTTGGGATAAAAGTTTCAGAGTTTACTCGACAGAAACAGGGAAACTGACACAGATTGTATT TGGCCACTGGGATGTTGTCACATGCCTGGCCAGGTCGGAGTCCTACATTGGTGGAGACTGCTACATAGTG TCTGGATCTCGGGACGCCACCTTGCTTCTCTGGTACTGGAGTGGGCGTCACCACATCATCGGAGACAACC CCAATAGCAGTGACTATCCTGCGCCCAGAGCTGTCCTCACAGGCCATGACCATGAAGTTGTCTGTGTCTC CGTCTGTGCAGAACTCGGACTCGTTATCAGTGGTGCTAAAGAGGGCCCTTGCCTCGTTCATACCATCACT GGAGATCTGCTGAGGGCCCTGGAAGGACCAGAAAACTGCTTATTTCCACGCCTAATTTCGGTATCCAGTG AAGGCCACTGCATCATATATTATGAGCGAGGACGGTTTAGCAACTTCAGCATCAATGGGAAACTTTTGGC TCAAATGGAGATCAATGATTCCACTAGGGCTATTCTCCTGAGCAGCGATGGACAGAACCTGGTGACTGGA GGGGACAATGGTGTGGTGGAGGTCTGGCAGGCCTGTGACTTTAAGCAGCTGTACATTTACCCAGGATGTG ATGCTGGCATTAGAGCGATGGATTTATCCCATGACCAAAGGACTCTGATCACTGGCATGGCTTCCGGCAG CATTGTAGCTTTTAATATAGATTTTAATCGGTGGCATTATGAGCATCAGAACAGGTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_030595
Insert Size	8811 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_030595.1, NP_085098.1
RefSeq Size	10998 bp
RefSeq ORF	8811 bp
Locus ID	26422
UniProt ID	Q9EPN1
Gene Summary	Binds to type II regulatory subunits of protein kinase A and anchors/targets them to the membrane. May anchor the kinase to cytoskeletal and/or organelle-associated proteins. May have a role in membrane trafficking.[UniProtKB/Swiss-Prot Function]

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