Megf8 (NM_001160400) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW049492; b2b288Clo; b2b1702.2Clo; b2b1702Clo; Egfl4; m687Ddg; mKIAA0817
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225411 representing NM_001160400 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTGGGTGGAGCTCTGGCCTTGGCCCTTGCACTGGCCTTGGCAGTGCTGGGACCGCTGTCCCTCA GGGTCCTGGCTGGTGACTGCAAGGGGCAGCGGCAGGTGCTACGGGAGGCGCCAGGCTTCGTGACGGATGG TGCGGGCAACTACAGCGTCAACGGCAACTGCGAGTGGCTTATCGAGGCCCCGAGCCCTCAGCACCGGATC CTGCTGGACTTCCTTTTCCTGGACACGGAGTGCACTTATGACTACCTCTTTGTGTATGACGGTGACTCGC CCCAAGGCCCTCTGCTTGCTAGTCTGAGTGGGAGCACCCGGCCCCCACCCATTGAGGCTTCCTCAGGCAA GATGCTCCTCCACCTCTTCAGCGATGCCAACTACAACCTGCTGGGCTTTAACGCCTCCTTCCGCTTCTCC CTGTGTCCCGGAGGCTGCCAGAACCATGGGCAGTGCAAGTCCCCGGGAGTGTGCGTTTGCGAGCCGGGCT GGGGAGGTCCAGACTGCGGCCTGCAGGAGTGCTCAGCCTACTGTGGCAGCCATGGCACCTGCGCCTCGAC CCTGGGACCATGCCGCTGTGAACCTGGATTCTTGGGACGAGCCTGTGACCTGCACTTGTGGGAGAACCAG GGGGCTGGTTGGTGGCACAGTGTGAGTGCCGGGGACCCTGCCTTCTCTGCCCGAATTGGGGCAGCTGGTG CCTTTCTGTCCCCACCAGGGCTGCTGGCTGTCTTTGGAGGCCAGGACCTTAACAAGGCCCTGGGTGACCT TGTCCTGTATAACTTCTCCACGAACACCTGGGAGTCCTGGGACCTGACGCCTGCTCCGGCTGCCCGTCAT TCTCATGTGGCCGTGGCCTGGGCTGGCTTGCTGGTGCTGATGGGTGGTGAGCTGGCCAATGGCTTGCTAA CCAATGATGTGTGGGCCTTTAGCCCCCTGGGTGGAGGCCACTGGGAGCTCCTGGCACCACCTGCTTCCAG TTCTTCTGGGCCCCCGGGGCTGGCAGGCCATGCAGCTGCTCTGGTGGATGACATCTGGCTTTATGTGTCC GGTGGCCGCACTCAGCATGACCTCTTTTCTTCTGGCCTCTTCCGGTTCCGCCTGGACCACACCAGCAGGG GCTATTGGGAGCAGGTGATCCCGGCAGGTGGGCGGCCCCCTGCTGCCACTGGGCACTCTATGGTGTTCCA TGCCCCATCTCGTACTCTGCTGGTCCATGGTGGACACCGGCCTTCCACTGCCCGGTTTTCTGTGCGGGTG AATTCCACTGAGCTCTTCCACGTGGAACGGCGTGTGTGGACCACGCTGAAGGGCCGGGATGGGCTCCAGG GTCCACGGGAGAGAGCCTTCCACACTGCCAGTGTTCTAGGAAACTACATGGTGGTCTATGGGGGCAATGT GCATACGCATTACCAGGAGGAAAAGTGTTACGAAGATGGCATCTTCTTCTACCACCTTGGCTGCCACCAG TGGGTGTCAGGGGCTGAGCTTGCCCCGCCAGGAACCCCTGAGGGTCGAGCGGCGCCTCCTAGTGGCCGTT ACTCACATGTGGCAGCTGTTCTTGGTGGCAGTGTCCTGCTGGTGGCTGGGGGATATAGTGGCCGGCCTCG TGGGGACTTGATGGCCTACAAGGTGCCCCCCTTCGTGTTTCAGGCACCTGCCCTAGATTACCACCTGGAC TATTGTTCCATGTACACAGACCACAGTGTCTGCTCCCGAGACCCGGAATGCAGCTGGTGTCAGGGCGCTT GCCAAGCTGCGCCCCCTCCTGGGACTCCCTCAGGAGCTTGTCCAGCGGCCAGCTGCCTGGGCCTAGGCCG CCTGCTAAGTGACTGCCAAGCATGCCTGGCCTTCAGCAGCCCCACAGCTCCTCCCCGGGGACCTGGCGCC CTAGGTTGGTGTGTGCACAATGAGAGCTGCCTTCCTCGGCCTGAGCAGGCCCGCTGCCGAGGGGAGCAGA TCTCAGGCACCGTGGGCTGGTGGGGGCCAGCACCCGTCTTCGTCACTTCCCTGGAGGCTTGTGTCACCCA GAGCTTCCTCCCTGGCCTGCACCTGCTCACCTTCCAGCAGCCACCCAACGCCTCTCAGCCTGACAAGGTC TCCATTGTCCGAAGTACAACTATCACCTTGACACCCAGCCCGGAGACAGATGTGTCCCTAGTCTACCGAG GCTTCATCCACCCCCTGCTGCCAGGAGGACCAGGCGGCCCTGGGGCTGAAGATGTGGCTGTGTGGGCTCG GGCCCAGCGTCTCCATGTCCTGGCCCGGATGGCCAGAGGCCCTGACACTGAGAACATGGAGGAGGTGGGA CGCTGGGTGGCTCAGCAGGAGAAGGAGACACGGCGTCTGCAGCGCCCTGGCTCTGATCGCCTCTTCCCAT TGCCGGGGAGAGGCAACAAGTACGCGGTGGAGATCCGAGGCCAGCTCAACGGCTCGGCAGGCCCTGGGCA TAGTGAGCTCACCCTCCTGTGGGATCGGACTGGAGTGCCGGGTGGCAGTGAGATCTCCTTCTTCTTCCTG GAGCCCTACCGCTCTTCGGCCTGCACCTCCTACTCCTCCTGTCTGGGCTGCCTGGCTGATCAGGGCTGTG GCTGGTGCCTCAACAGTGCCACCTGTCACCTGCGTCAAGGCAGGGCCCACTGTGAGGATGATGGGAGCGG CGAGTCCCTGCTGGTGCTGGTGCCTGCCCTCTGTCCACTCTGTGAGGAGCATCGTGACTGTCATGCCTGC ACCCAGGACCCCTTCTGTGAGTGGCATCAGAGCACCAACCGCAAAGGAGATGCAGCGTGCAGCCGGCGAG GCCGGGGCCGAGGCGCTCTGAAGAACCCAGAGGAATGCCCCCCACTCTGCAGCCAACGGCTGACCTGTGA GGACTGCCTGGCCAACTCCAGCCAGTGTGCCTGGTGCCAGTCTACTCACACCTGCTTCCTGTTTGCTGCC TACCTGGCCCGGTACCCACATGGGGGCTGCCGTGGCTGGGATGACAGTGTGCACTCAGAGCCACGGTGCA GGAGCTGCGGTGGCTTTTTGACTTGTCATGAGTGTCTGCAGAGCCACGAGTGTGGTTGGTGTGGCAACGA AGACAACCCCACTCTGGGACGGTGCCTACAAGGGGACTTCTCTGGACCCCTGGGTGGGGGGAACTGCTCC CTCTGGGTGGGAGAGGGCCTGGGCCTGCCTGTGGCTCTCCCTGCCCGCTGGGCTTATGCCCGCTGTCCAG ATGTGGATGAATGTCGCCTGGGCCTGGCGAGGTGTCACCCACGGGCAACCTGCCTGAACACGCCTCTCAG CTATGAGTGTCATTGCCAGCGTGGCTACCAGGGCGATGGCATCACACACTGTAACCGCACGTGCCTGGAA GACTGTGGCCACGGTGTGTGTAGCGGTCCTCCGGACTTCACTTGTGTGTGTGACCTGGGCTGGACCTCAG ACCTGCCCCCACCCACGCCTGCCCCAGGACCCCCTGCCCCTCGTTGCTCTCGGGACTGTGGCTGCAGCTT TCATAGCCACTGCCGCAGGCGGGGACCTGGCTACTGTGACGAGTGCCAGGACTGGACGTGGGGGGAACAC TGTGAACGGTGCCGGCCTGGCAGCTTTGGCAATGCAACCGGCTCTGGTGGCTGCCGACCCTGCCAGTGTA ACGGGCATGGGGACCCACGTCGTGGCCACTGTGACAACCTCACTGGGCTCTGCTTCTGCCAGGACCACAC TGAGGGTGCCCACTGTCAGATTTGCTCTCCAGGATACTATGGGGACCCCCGAGCTGGTGGCTCCTGCTTC CGGGAGTGTGGGGGTCGTGCGCTCCTCACCAATGTGTCCTCGGTGGCTCTGGGCTCCCGCCGCTTTGGGG GACTTCTGCCTCCAGGTGGTGGGGCAGCAAGAGCTGGGCCCGGGCTGTCCTACTGCGTGTGGGTTGTCTC TGCCACAGAAGCGCTGCAGCCCTGTGTCCCTGGCACCCTCTGCCCCCCGCTCACCCTCACCTTCTCTCCA GACAGCAGCACGCCTTGCACACTGAGCTACGTCCTGGCCTTTGATGGATTCCCACGCTTCCTGGACACTG GTGTTGTTCAGTCAGACCGGAGCCTCATAGCTGCCTTCTGCGGCCAGCGGCGTGACAGACCTCTCACTGT GCAGGCTTTATCAGGGTTACTTGTGTTGCACTGGGAAGCCAACGGCTCCTCCTCCTGGGGATTCAATGCT TCTGTGGGCTCTGCCCGGTGTGGATCAGGAGGTCCTGGCAGTTGCCCTGTCCCTCAGGAATGTGTGCCCC AGGATGGGGCTGCAGGTGCTGGCCTCTGCCGCTGTCCTCAGGGCTGGGCTGGCCCTCACTGCCGCATGGC TCTGTGTCCTGAGAATTGCAACGCCCACACCGGGGCAGGGATTTGTAACCAGAGCCTAGGTGTGTGCATC TGTGCTGAGGGCTTTGGCGGCCCAGACTGTGCCACGAAGCTGGATGGTGGACAGTTGGTCTGGGAAACGC TCATGGACAGCCGCCTCTCAGCTGATACTGCTAGTCGCTTCCTTCACCGCCTGGGACATACTATGGTAGA GGGGCCTGATGCCACTTTGTGGATGTTTGGGGGCCTGGGTCTGCCACAGGGCCTGCTGGGAAACCTGTAC AGGTATTCAGTCAGTGAGCGCAGATGGACACAGATGTTGGCAGGAGCTGAGGATGGGGGCCCAGGCCCAT CACCCCGTTCCTTCCATGCAGCCGCCTATGTGCCTGCTGGCCGAGGTGCCATGTACCTGCTGGGGGGCCT CACAGCTGGAGGCGTCACCCGTGACTTCTGGGTCCTTAACCTCACTACCCTGCAGTGGAGACAGGAGAAG CCCCCTCAGAACATGGAGTTGCCAGCGGTCGCTGGTCACACCCTTACTGCCCGTCGAGGCCTATCTCTGC TCTTGGTGGGTGGCTACTCCCCCGAAAATGGCTTCAACCAGCAGTTGTTGGAGTACCAGCTAGCAACCGG CACCTGGGTATCTGGAGCCCAGAGTGGGACACCCCCAACAGGTCTCTATGGTCATTCTGCAGTCTACCAC GAGGCCACCGACTCCCTCTATGTGTTTGGGGGTTTCCGCTTCCACGTGGAGCTGGCAGCACCTTCTCCGG AGCTCTACTCCCTGCACTGTCCTGACCGTACCTGGAGCCTGCTGGCACCTTCTCAGGGGGCAAAGCCCCG ACCCCGGCTTTTCCACGCTTCAGCTCTGCTCGGAGACACCATGGTGGTCCTTGGGGGGCGCTCGGACCCT GATGAATTCAGCAGTGATGTTCTGCTCTACCAGGTCAACTGCAACACCTGGCTCCTGCCTGCCCTCACCC GTCCAGCCTTTGTGGGGTCCCCGATGGAGGAGTCTGTGGCCCATGCAGTGGCAGCAGTTGGTAGCCGCCT GTACATCTCTGGAGGATTTGGGGGAGTAGCCTTGGGCCGCCTGCTGGCATTGACCTTGCCCCCCGACCCC TGCCGTCTGCTGCCTTCACCTGAAGCTTGTAATCAGTCTGGGGCCTGCACCTGGTGCCATGGTGCTTGCC TGTCTGGGGACCAGGCCCACAGGCTGGGTTGTGGGGTGCCCCCTTGCTCCCCAATGCCTCGCTCGCCTGA GGAATGTCGGAGGCTGCGGACCTGCAGTGAGTGCCTGGCCCGCCATCCTCGGACCCTGCAGCCTGGAGAT GGAGAGGCATCCATTCCCCGTTGTAAGTGGTGTACCAACTGCCCAGAGGGTGCCTGCATTGGGCGCAATG GGTCCTGCACCTCAGAGAATGACTGCCGAATCAACCAGCGAGAGGTCTTCTGGGCTGGCAACTGCTCAGA GGCTGCGTGTGGGGCTGCTGACTGTGAGCAGTGCACGCGGGAGGGCAAGTGCATGTGGACGCGGCAGTTC AAGAGGACCGGGGAGACTCGCCGAATCCTGTCAGTGCAGCCCACCTATGACTGGACTTGCTTCAGCCATT CCCTGTTGAATGTGTCTCCTATGCCAGTGGAGTCCTCGCCTCCACTGCCCTGCCCCACGCCCTGTCACCT CCTGCCCAACTGCACATCGTGCCTGGCCTCCAAGGGTGCAGATGGGGGCTGGCAGCACTGTGTGTGGAGC AGCAGCCTCCAGCAGTGTCTGAGCCCCTCCTATCTGCCCTTGCGATGTATGGCTGGAGGCTGTGGGCGGC TGCTTAGAGGGCCAGAGAGCTGCTCGCTGGGCTGTGCTCAGGCGACACAGTGTGCCCTGTGCCTGCGACG ACCCCACTGTGGCTGGTGTGCCTGGGGAGGCCAGGATGGGGGTGGCCATTGCATGGAAGGAGGTCTCAGT GGTCCCCGAGACGGGCTAACGTGTGGACGTCCCGGGGCCTCCTGGGCCTTCCTGTCTTGCCCCCCTGAGG ACGAGTGTGCAAATGGGCACCATGACTGCAATGAGACCCAGAACTGCCATGACCAGCCCCACGGCTACGA GTGCAGCTGCAAGACAGGCTACACCATGGACAATGTGACTGGCGTCTGCCGCCCTGTGTGCGCCCAGGGC TGTGTGAACGGATCCTGTGTGGAGCCTGATCACTGCCGCTGTCACTTTGGCTTTGTTGGCCGCAACTGCT CCACAGAGTGTCGCTGCAACCGCCACAGTGAGTGTGCCGGCGTTGGAGCACAGGACCACTGCCTGCTGTG CCGGAACCACACCAAGGGTAGCCACTGTGAGCAGTGTCTCCCACTGTTCGTGGGTTCGGCTTTGGGCGGT GGGACCTGCCGACCCTGCCATGCCTTCTGTCGCGGGAACAGCCATGTGTGCGTCTCCAGGAAAGAGCTAG AAATGGCCAGAAAGGAGCCAGAGAAGTACTCACTGGATCCAGAGGAGATTGAAACCTGGGTGGCAGAAGG TCCCAGTGAAGATGAAGCTGTGTGTGTGAACTGTCAGAACAACAGCTACGGGGACAGATGTGAGAGCTGC CTCCACGGCTACTTTCTCCTGGACGGGAAATGCACCAAGTGCCAGTGTAATGGCCACGCTGACACCTGTA ACGAGCAGGACGGGACAGGATGCCCATGTCAGAACAACACAGAGACAGGCACCTGCCAGGGCAGCTCTCC CAGTGACCGCCGAGACTGCTACAAGTACCAGTGTGCTAAGTGCCGCGAGTCATTCCACGGGAGCCCGCTA GGTGGCCAGCAGTGTTACCGACTCATCTCTGTGGAACAAGAGTGCTGCCTGGACCCCACTTCCCAGACCA ACTGCTTCCATGAGCCAAAGCGCCGGGCCCTGGGTCCTGGCCGCACCGTGCTGTTTGGCGTCCAACCCAA GTTCACCAATGTGGACATTCGCCTGACACTAGACGTGACCTTCGGCGCTGTGGACCTCTATGTCTCCACC TCCTATGACACTTTTGTGGTCCGAGTGGCCCCTGACACAGGCGTCCACACTGTGCACATCCAGCCACCTC CACCTCCCCCACCTCCCCCGCCTCCTGCTGATGGTGTACCTCGGGTGGCTGCGGACCTAGGAGGATTGGG GACTGGAAGTGGGTCAGGATCTCCGGTGGAGCCACGGGTCCGGGAGGTGTGGCCACGGGGCCTGATTACC TACGTCACAGTGACGGAACCCTCGGCAGTGCTGGTGGTCCGCAGTGTGAGGGACAGGCTGGTGATCACCT ATCCCCATGAGCACCACGCCCTCAAATCCAGCCGCTTCTACCTGCTGCTGCTGGGTGTAGGAGACCCCAA CGGGCCTGGTGCCAACGGTTCAGCAGACTCACAGGGCCTGCTCTTCTTCCGGCAAGACCAGGCCCACATT GACCTGTTCGTCTTCTTCTCTGTCTTCTTTTCCTGCTTCTTCCTCTTCCTCTCACTCTGTGTGCTCCTCT GGAAGGCCAAGCAGGCTCTGGACCAGCGACAAGAGCAGCGCCGCCACCTGCAGGAGATGACCAAGATGGC CAGCCGCCCCTTTGCCAAGGTCACTGTCTGCTTCCCTCCAGACCCTGCAGGCCCAGCACCTGCATGGAAG CCAGCTGGGCTCCCTCCTCCTGCCTTCCGCCGCTCTGAGCCTTTCCTAGCACCCCTGTTGCTGACAGGAG CCGGGGGTCCCTGGGGACCCATGGGAGGCGGGTGCTGCCCACCAGCCCTCCCTGCCACCACAGCTGGCCT ACGAGCTGGACCCATCACCCTTGAGCCCACAGAAGATGGCATGGCTGGTGTGGCCACTCTGCTGCTCCAG CTGCCTGGGGGTCCTCATGCTCCCAATGGGGCCTGCCTTGGGTCAGCCCTCGTCACTCTGCGTCATAGGC TCCATGAGTACTGTGGGGGCAGCGGGGGTGCTGGAGGCAGTGGACACGGGGGTGGTGGAGGCCGGAAGGG ACTGTTGAGTCAGGACAATCTCACTAGCATGTCCCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001160400
Insert Size	8370 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001160400.1, NP_001153872.1
RefSeq Size	10040 bp
RefSeq ORF	8370 bp
Locus ID	269878
UniProt ID	P60882
Gene Summary	Acts as a negative regulator of hedgehog signaling (PubMed:29290584).[UniProtKB/Swiss-Prot Function]

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