Nsd1 (NM_008739) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI528500; KMT3B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225389 representing NM_008739 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGGACTTGTGAATTATCTAGAAGAAATTGTCTGCTGTCCTTTTCTAATCCGGTGAATTTAGATG CCTCCGAAGACAAGGACAGCCCTTTCGGTAATGGTCAATCCAATTTTTCTGAGCCACTTAATGGGTGTAC TATGCAGTTACCGACTGCTGCCAGTGGAACATCCCAAAATGCTTATGGACAAGATTCTCCATCTTGTTAC ATTCCACTGCGGAGACTACAGGATTTGGCCTCCATGATCAATGTAGAATATTTAAGTGGGTCTGCTGATG GATCAGAATCCTTTCAAGACCCTGCAAAAAGTGATTCAAGAGCTCAGTCGCCAATTGTTTGCACTTCCTT GAGTCCTGGTGGTCCAACAGCACTTGCTATGAAACAGGAGCCCACTTGTAATAACTCCCCCGAACTCCAG TTAAGAGTAACAAAGACGACCAAGAATGGCTTTCTGCACTTTGAGAATTTTACTGGTGTGGACGATGCAG ATGTAGATTCTGAAATGGACCCAGAACAGCCAGTCACAGAGGATGAGAGTATAGAGGAGATCTTTGAGGA GACTCAGACCAATGCCACCTGCAATTATGAGCCTAAATCAGAGAATGGTGTAGAAGTGGCCATGGGAAGT GAACAAGACAGCATGCCAGAGAGTAGACATGGTGCAGTGGAACGGCCATTCTTGCCGTTAGCTCCTCAAA CTGAAAAACAGAAAAATAAACAAAGAAGTGAAGTGGACGGAAGCAATGAAAAGACAGCCCTCCTCCCAGC CCCCACCTCACTAGGAGACACGAACGTTACCGTAGAAGAGCAATTTAACTCAATAAATTTATCTTTTCAG GATGATCCAGACTCCAGTCCCAGTCCATTAGGAAACATGCTAGAAATACCTGGAACTTCATCACCATCCA CTTCACAGGAATTGCCATTTTGTCAACCGAAGAAAAAGTCTACACCACTGAAATATGAAGTTGGAGATCT TATTTGGGCAAAATTCAAGAGACGCCCTTGGTGGCCCTGCAGGATCTGTTCTGATCCATTGATTAATACA CACTCAAAAATGAAAGTTGCCAATAGGAGGCCATATCGCGAATACTACGTAGAGGCTTTTGGAGACCCTT CTGAAAAAGCCTGGGTGGCTGGAAAAGCAATCGTCATGTTTGAAGGCAGACATCAATTTGAAGAACTACC TGTCCTTAGGAAAAGAGGGAAACAGAAAGAAAAAGGATATAGGCATAAGGTTCCTCAGAAGATTTTGAGT AAATGGGAAGCCAGCGTTGGTCTTGCCGAGCAATATGATGTTCCCAAAGGCTCTAAGAACCAAAAGTGTG TCAGTAGCTCAGTCAAGTTGGACAGTGAGGAAGATATGCCATTTGAGGACTGTACAAACGATCCTGATTC AGAACACCTGTTGCTTAATGGCTGCTTGAAGTCTCTGGCTTTTGATTCTGAGCATTCTGCAGATGAAAAG GAAAAGCCCTGTGCCAAGTCTCGAGTTAGAAAAAGCTCTGATAATATAAAAAGGACTAGTGTGAAAAAGG ATCTGGTGCCATTTGAATCACGTAAAGAAGAACGGAGGGGGAAAATTCCAGACAACCTTGGCCTAGACTT TATCTCTGGGGGTGTATCTGATAAACAGGCTTCTAATGAACTTTCCAGGATAGCAAACAGCCTCACAGGG TCTAGCACTGCACCAGGAAGTTTCCTCTTTTCTTCCAGTGTACAGAACACTGCTAAGACAGACTTTGAGA CTCCAGATTGCGATTCCTTATCAGGTTTGTCTGAGAGTGCTTTAATCTCTAAACATTCTGGAGAGAAAAA GAAACTCCAACCAGGCCAGGTCTGCAGCTCAAAAGTACAGCTCTGCTACGTTGGAGCTGGTGATGAGGAA AAGCGAAGCAATTCTGTCAGTGTGAGTACCACTTCTGATGATGGCTGCAGTGACCTGGATCCTACAGAGC ACAACTCAGGATTCCAGAACAGTGTCCTTGGAATTACAGATGCTTTTGATAAAACAGAGAATGCTTTATC GGTGCACAAAAATGAAACACAGTATTCTAGATATCCTGTCACAAACAGGATAAAAGAGAAACAGAAATCC CTCATTACTAATTCCCATGCAGACCATTTAATGGGTTCTACCAAGACAATGGAGCCTGAAACTGCTGAGT TGTCTCAGGTCAATCTTTCTGATCTTAAAATTAGTAGTCCTATTCCCAAACCCCAACCAGAATTTAGGAA TGATGGTCTCACTACAAAATTCAGCGCGCCACCAGGCATTCGTAATGAAAATCCACTGACAAAGGGTGGG CTTGCAAATCAAACTCTATTACCTCTGAAATGCAGACAGCCCAAGTTTCGTAGTATAAAATGCAAGCATA AAGAAAGCCCAGCTGTTGCAGAAACCTCAGCTACAAGTGAGGACCTCAGTTTGAAATGCTGTTCTTCTGA TACCAATGGCTCTCCTTTGGCCAATATATCCAAAAGTGGGAAAGGAGAGGGGCTAAAACTACTGAACAAT ATGCATGAGAAAACCAGAGATTCTAGTGACATAGAAACAGCAGTGGTGAAGCACGTTCTTTCGGAACTGA AGGAGCTCTCGTACAGATCATTAAGTGAAGACGTAAGTGATTCTGGAACGGCAAAGGCATCAAAGCCATT ACTGTTTTCTTCTGCTTCTAGTCAGAATCATATACCAATTGAACCAGATTACAAGTTTAGCACATTGCTG ATGATGCTGAAGGATATGCATGACAGTAAGACAAAGGAGCAGCGGTTAATGACTGCTCAAAATCTGGCTT CCTACAGGACTCCAGATCGTGGGGACTGTTCTTCTGGTAGCCCTGTAGGGACATCAAAAGTTTTGGTGTT AGGAAGCTCCACACCCAATTCTGAAAAGCCTGGAGATAGCACTCAGGACTCAGTTCACCAGAGTCCTGGT GGGGGTGACTCTGCCTTGTCTGGAGAGTTGTCTTCTTCCCTGTCCAGCTTAGCTTCTGACAAAAGAGAGC TCCCTGCTTGTGGTAAAATTCGCTCTAACTGTATCCCAAGGCGCAACTGTGGCCGAGCAAAGCCATCATC CAAGTTGAGAGAAACCATTTCAGCCCAGATGGTAAAACCTTCAGTAAACCCAAAAGCCTTAAAGACTGAG CGGAAGAGAAAGTTCAGCCGACTTCCAGCTGTGACACTTGCTGCTAACAGACTGGGAAACAAAGAAAGTG GATCAGTGAATGGCCCATCCAGGGGTGGGGCTGAAGATCCTGGTAAAGAAGAGCCTCTGCAACAAATGGA CCTTTTAAGAAATGAAGACACACATTTTTCAGATGTACATTTTGATAGCAAGGCTAAACAATCGGACCCT GATAAAAATCTTGAAAAAGAACCTTCTTTTGAGAACAGAAAAGGCCCAGAGTTGGGCTCTGAAATGAACA CTGAGAATGATGAACTCCATGGTGTTAATCAGGTGGTGCCTAAAAAGCGGTGGCAGCGATTAAACCAAAG GCGCCCTAAGCCCGGAAAGCGTGCTAACAGATTTAGGGAGAAAGAAAACTCGGAGGGTGCTTTTGGAGTC TTACTTCCTGCTGACGCTGTGCAGAAGGCCCGGGAAGACTACTTAGAACAAAGAGCTCCTCCTACAAGTA AACCTGAGGACTCAGCAGCAGATCCCAATCATGGCAGCCACTCTGAATCGGTTGCGCCTCGGCTGAATGT TTGTGAGAAGTCCAGTGTTGGCATGGGTGATGTAGAAAAGGAGACAGGAATTCCCAGTTTGATGCCACAG ACCAAGCTCCCTGAGCCAGCTATTCGGTCAGAGAAGAAACGCCTCAGGAAGCCGAGCAAGTGGCTTCTGG AATATACAGAAGAATATGATCAGATATTTGCTCCTAAGAAAAAACAAAAGAAGGTACAGGAACAAGTGCA TAAGGTAAGTTCGCGCTGTGAAGACGAAAGCCTTCTTGCCCGATGTCAACCTAGTGCTCAGAACAAGCAG GTGGATGAGAATTCTTTGATTTCAACTAAAGAAGAGCCTCCAGTTCTTGAACGAGAGGCTCCATTTTTGG AAGGGCCATTGGCTCAGTCAGATCTTGGAGTTACACATGCTGAGTTGCCACAGCTGACCTTGTCTGTTCC TGTGGCTCCAGAAGCCTCTCCGCGGCCTGCCCTTGAGTCTGAGGAATTGCTCGTTAAGACACCAGGAAAT TATGAAAGTAAGCGTCAAAGAAAACCAACTAAGAAACTTCTTGAATCTAATGATTTAGACCCTGGATTTA TGCCCAAGAAGGGTGACCTTGGCCTTTCTAGAAAGTGCTTTGAAGCCAGTCGCTCGGGAAATGGTATTGT TGAATCACGGGCTACATCTCATTTGAAAGAGTTCAGTGGAGGCACTACCAAGATCTTTGATAAACCAAGG AAACGGAAACGGCAGAGGCTTGTCACAGCCAGAGTGCACTATAAAAAAGTGAAGAAGGAGGACTTAACAA AAGACACACCCAGCTCGGAGGGAGAACTGCTGATTCACAGGACTGCTGCCAGCCCCAAGGAGATTCTTGA AGAGGGTGTAGAACATGATCCTGGGATGTCTGCATCCAAGAAACTGCAGGTTGAAAGAGGCGGAGGAGCG GCACTCAAGGAGAATGTGTGTCAGAACTGTGAGAAGCTGGGTGAGCTGCTGTTGTGTGAGGCTCAGTGCT GCGGGGCTTTCCACTTGGAGTGCCTTGGCCTCCCCGAGATGCCTCGAGGGAAATTTATCTGCAACGAGTG TCACACAGGAATCCATACCTGTTTTGTATGTAAGCAGAGTGGAGAAGATGTGAAAAGGTGCCTTCTACCC TTATGTGGGAAATTTTACCATGAAGAGTGTGTCCAGAAGTACCCACCGACTGTCACACAGAACAAGGGCT TCAGGTGCCCCCTCCACATTTGTATAACCTGCCATGCTGCTAATCCAGCCAATGTATCTGCTTCTAAAGG TCGTCTGATGCGCTGTGTCCGCTGTCCTGTGGCATACCATGCTAATGACTTTTGTCTTGCTGCTGGTTCA AAAATCCTTGCATCTAATAGTATCATCTGCCCTAATCACTTCACCCCTAGGCGGGGCTGCCGAAATCATG AGCATGTTAATGTCAGCTGGTGTTTTGTGTGTTCAGAAGGAGGCAGCCTTCTGTGCTGTGATTCTTGCCC TGCTGCTTTTCATCGAGAATGCCTAAATATCGATATCCCTGAAGGAAATTGGTACTGCAATGACTGTAAG GCAGGCAAAAAGCCACACTACAGGGAGATTGTCTGGGTAAAAGTTGGTCGCTACAGGTGGTGGCCAGCTG AGATCTGCCATCCTCGAGCTGTTCCCTCTAACATTGATAAAATGAGGCACGATGTGGGAGAATTCCCTGT TCTCTTCTTTGGGTCCAATGACTATCTGTGGACTCATCAGGCTCGAGTCTTCCCTTACATGGAAGGGGAT GTAAGCAGCAAGGATAAGATGGGAAAAGGAGTTGATGGAACATATAAAAAAGCTCTTCAGGAAGCTGCAG CAAGATTTGAAGAGTTAAAAGCTCAAAAAGAATTAAGACAGCTGCAGGAAGATCGGAAGAATGACAAGAA GCCGCCACCTTACAAACATATAAAGGTGAACCGGCCTATTGGCAGGGTACAGATCTTCACTGCAGATTTG TCAGAAATTCCCCGTTGCAATTGTAAAGCTACTGATGAGAACCCCTGCGGGATAGACTCAGAGTGTATCA ACCGGATGCTGCTCTATGAGTGCCACCCAACAGTGTGTCCTGCAGGAGTTCGCTGTCAGAACCAGTGCTT CAGCAAGCGCCAGTATCCAGATGTTGAAATTTTCCGCACATTACAAAGGGGCTGGGGTCTCCGAACAAAA ACGGATATTAAAAAGGGAGAATTTGTGAATGAATATGTGGGTGAGCTAATAGATGAAGAAGAGTGCAGAG CTCGGATCCGTTACGCACAAGAGCACGACATCACTAATTTCTATATGCTCACGCTAGATAAAGATCGAAT CATTGATGCTGGTCCTAAAGGGAACTACGCACGCTTCATGAATCACTGCTGCCAGCCTAATTGTGAGACA CAGAAGTGGTCGGTGAATGGAGATACCCGTGTTGGTCTTTTTGCTTTGAGTGACATTAAAGCAGGCACTG AACTTACCTTCAATTACAACCTAGAGTGTCTTGGGAATGGAAAAACCGTCTGTAAATGTGGAGCACCCAA CTGCAGTGGCTTCTTGGGTGTGAGGCCAAAGAATCAACCCATTGTCACAGAAGAAAAGTCAAGGAAATTC AAAAGGAAGCCACATGGGAAGCGCAGGAGCCAGGGCGAAGTCACCAAGGAAAGAGAGGATGAATGTTTCA GCTGTGGGGATGCTGGTCAGCTCGTCTCCTGCAAGAAGCCAGGCTGCCCTAAAGTCTACCATGCAGACTG TCTGAATCTGACAAAGCGACCAGCAGGGAAGTGGGAATGTCCTTGGCACCAGTGTGACGTGTGTGGGAAG GAAGCAGCCTCCTTCTGTGAGATGTGCCCCAGCTCCTTTTGCAAGCAACATCGAGAAGGGATGCTTTTCA TTTCCAAGCTCGATGGGCGTCTGTCTTGTACTGAACATGATCCCTGTGGGCCCAACCCTCTGGAACCTGG GGAGATCCGTGAGTATGTGCCTCCCACAGCGACGAGTCCTCCAAGCCCCGGCACTCAGCCAAAAGAGCAG TCATCAGAGATGGCTACTCAGGGGCCCAAGAAGTCTGATCAGCCACCTACTGATGCCACCCAGTTGCTGC CACTCTCCAAAAAAGCCCTAACAGGGTCTTGTCAGAGGCCACTGCTCCCTGAAAGGCCTCCTGAGAGAAC TGACTCTAGTTCCCACCTTTTAGATAGGATCAGAGACCTTGCTGGGTCAGGGACCAAATCCCAGTCCTTG GTATCCAGCCAGAGACCACAGGACAGGCCACCTGCTAAAGAAGGACCAAGACCTCAGCCACCTGACAGAG CCTCTCCAATGACCAGGCCAAGCTCATCACCTTCAGTCAGCTCCCTGCCACTGGAAAGACCTCTAAGAAT GACTGACTCCAGGCTGGATAAATCCATAGGTGCTGCCAGCCCAAAGTCCCAGGCAGTGGAGAAAACTCCA GCCTCCACTGGCCTGAGACTTTCATCACCAGACAGGCTGCTAACCACTAACAGTCCCAAACCCCAGATTT CTGACAGGCCCCCAGAGAAGTCCCATGCCTCTTTGACCCAGAGACTTCCACCTCCTGAGAAAGTGCTATC AGCTGTGGTCCAGTCTCTGGTAGCTAAAGAGAAAGCCCTGAGGCCTGTGGACCAGAACACCCAATCAAAG CACAGACCTGCTGTGGTGATGGATCTCATAGACCTAACGCCTCGCCAGAAGGAGCGGGCTGCTTCTCCTC AGGAAGTCACCCCACAGGCTGATGAGAAGACAGCAATGCTGGAGTCAAGCTCATGGCCGTCCAGCAAAGG TCTGGGGCATATCCCACGAGCTACGGAGAAGATCAGCGTGTCAGAGTCCCTCCAGCCCTCGGGAAAAGTA GCAGCTCCTTCAGAACACCCTTGGCAAGCTGTTAAATCGCTCACCCATGCCAGATTTCTTTCTCCGCCTT CGGCCAAGGCTTTTTTATATGAATCAGCAACTCAAGCCTCAGGAAGAACCCCTGTGGGAGCTGAGCAGAC CCCAGGACCTCCTAGCCCAGCACCAGGCCTGGTTAAACAGGTGAAACAGCTATCTAGAGGACTCACTGCC AAGAGTGGGCAGTCCTTCAGGTCTCTTGGGAAGATCTCAGCTTCCCTCCCCAATGAAGAAAAGAAGTTGA CAACCACAGAACAGAGTCCCTGGGGCCTGGGAAAAGCCTCACCAGGGGCAGGGCTTTGGCCTATAGTTGC TGGACAAACACTAGCCCAGGCTTGCTGGTCTGCTGGGGGCACACAAACTTTGGCACAAACTTGCTGGTCT CTTGGAAGAGGGCAAGACCCCAAACCAGAAAATGCAATTCAAGCTCTTAACCAGGCTCCTTCTAGTCGCA AATGTGCAGATTCAGAAAAGAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_008739
Insert Size	8076 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_008739.3, NP_032765.3
RefSeq Size	12784 bp
RefSeq ORF	8076 bp
Locus ID	18193

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.