Cep295 (NM_176976) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5830418K08Rik; 5832426L23Rik; Gm1131; Kiaa1731
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225300 representing NM_176976 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGAAGTGACGAGAGCCGGGTCATGGCTGTCCCGCAGGTACCCAAACGGCTGCTGGGTAGCCGGCC GGGTGCGGGTGCCTGTGTGTCGTGAGGCCAGACCACACACGCTGTCAGATCCTTTATATTTAACATACTC AGAAATGAAAAGAAAGGGAATGAATACAAAGCTGAGATTGAGCCCTAGTGAAGAAGCCTTCATTTTGAAG GAAGATTATGAAAGAAGACGAAAACTAAGATTGCTGCAGGTTCGAGAACAAGAAAGGGGCATTGCCTTCC AGATAAGAGAAGGCATAAAACAGAGGCGAAGTCAGCAGGTTTCTCATTTGGCTGAGGAGCTAAGGGCAAA ATGGGAAGAAGCACAAAGCCAGAAAATACAGAACTTGGAAAAACTATATTTGGCAAGTTTAAGACATATG GGAGATGGACATCGACAGGCTAAAGAAAATGAACCTGACTTGGATGCTCTTTCTCGGCGGGCAGCAGAAA GAAAAAGAAAAGCAGAAGTGAGGCATAAAGAAGCCCTAAAAGTGCAGAAAAATCAAAAAGAAATGTTAAT GAAACAGAAAACCAGGCACATAAAAGCTCGAAAGGAAGCAGTGCTTGTGGAAAAACAGAGATCTGCTAAA ATGGCCAGGCTGCCACCGCCTGTCCCATCCCCCTTTGAGAACATTGACGTGAACAGAATTCCTTCATTGA AAACTAACAGTTCCACTTACCATCATATTTCCACCTTTGTGAGTAGACAGATGGGTACAAAGCAGCCAGA TGCTCATTTGGCTGCTGAAGAAGAAGCTAGACGAGTGGAAAGACTACGGAAACAGGCGGCACAGGAGAGA GTGAAACAGTTTGAAAGGGCACATGTGCGGGGATCCCAAGCCATGAAAAAGATCCACTTGGCCCAAAACC AGGAGCGATTGATGGAAGAACTCAAACAGCTGCAGAAAGAGGACCTGGCCCGTAGGAGGCAGACCGTGGC ACAGATGCCGCCACAGATGCTGGAGTTGCCATACCGACGGAGTGAGATGAAGGAAGACAGGCAGCGAGAG CTGGAGTTTGCCTTTGAAGATATGTACAATGCAGATAGGAAGGTGAAAGGAAACCTGATTTTACATCTTA AGCCAGAACCTTTGCCTACAATCTCTGATCAGCTCCAAGATGAAGAACTGGACCTTTCAATGGAACAAGA AAATCAAGTGCCCTTGGCAGCAAAGATCCAACAAATCCCCTCAAGAATTCTTTTTAAAAGATTATTAAAC AAGATTAGAAGTCAAAAATCTCTCTGGACAATTAAATCTGTGTCTGAGGATGAAGGTGAGGTGACTTCGA GCATCATTGAGATTGAGAGTAAAGTGCCAAGCGTGGACTCGGGAGCCATTATCACAGAGGAGAGAACAGC GGCGTCCTTTGAGCAGGAGCAAGTCACAGACAGTGATAGGCTGACGATAGAATCTGGACCACTGAGCAGT GAAGATAAGCCATTGTACTACAAAGCGGGGACTGGAAGGGAACAAGCAATGGCAGTGTCTCCACCTGCCA CAGCTGTGGCTCAGAGTTCAGTCCTGCTTCATCCTCAGGAAGAGGCAGTCAGAATTCGAATGTCATTAAG GCGCAAACAGATAATGGAGATAGAAGAGCAGAAGCAGAAGCAGTTGGAATTACTTGAACAAATTGAGCAA CAGAAGTTAAGGTTAGAAACTGATTGTTTCCGGGCTCAACTGGAAGAGCAAAGAAAGCAAGCTGATCAGC CTGAGGTTTGCTGTGCTCCAATGTCACATGCTATGATTTCTGATGAAGACTCTCACAGGCAGATGATTCG TAACTATCAACATCAGCTTTTACAACAAAACAGGTTACACAAGGAGACTGTTGAAACTGCCAGGAAACGA TTACTCGAATACCAAACTGTATTAAAAGAAAGATCCCCATCCCTGTCAGCCTCAGCACTGGTACCTGATT CTGTTGTGTCAGGGCCACCACAGCAATCCTACAAGCCTGCTGCTGCTTCAGACTCTTGGGATCCAAGCCA GAGACTGAAACTAAGTCCTAGCAAATATCAACCTGTGCAACCCTCACAGATCCCTGCATTAGAGCAGAGT CATATTCAGGTACCAAGACATGGTCACATTACACAGAGGCAAGGAAAAATGGCTGTATCAGAAATGTTAG GTAAGCAGCCCGTGGAGTCACAAGAACGTCAGTGGCAGTTCTCTCAGGTGGAAACACATCAGGGAGACTA TGAGTTCGTCCTTAAAGATTCTCATTCACTTTCAAGGACTTTGTCTTATGTTAGGCCACAAACATTACAA GATGCTAGAGAAGTATCTAAACCTCCTAGGGTAATAATTTGTCAAAGTCTAGATTCCCAACAGATATCAT CAGAGGATAGTGAAAATATATCTTCTAAGCCAAGTGAACCGTCTCCTTTTCTCCCACTGGTGCCTGAACG CCCTTTTACTAGTCTCCCAGTTAAATTCCATTCTGGAACAATTCACAAACCCTTTACGACCATAAACCAG TCTGTAATCAGTCAAATGCATGATCAACCTTTGTCATCCTCAGAGACTATTACAGCTCAGCAGGGTGACT TAAGATTTCTTCAAGAACAGTTAGAACTACAGAAGAAAGTTCTTCAGGCAAGACAAGAGGCTCGGGAAAA GCTGCTTTTGTGCACACAGAAAGAACTGGGACAGCAGACTGGCCTCCCAGTGTTCCTCCCTTCACCAGCT GGAAATATATTTTCTTCATTACCCTCTGCCTCAGCTGAATCAGGAAACTTTCAGACATCTTCAACAAAAA GTGATGCCACTGTTTCCTCAGACAATATGGACAGGCTTTGGGATTCTTCACAACCCATCTCATCACAGCA AACTCATTTAGAATTTCTCCAAGAGCAGTCCAGTGTTGAGACGGATAACCTTCAGGCTAGGCGGGAAGCC CAGGAGGTCTTGTTTGCACATACACAAAATACGCTGGAAAAAATTGTACGTTCTGAACAAGCTGGGTCTT CTTTGCCACATCAGGTAGCTCAGCAGTCATTTAGTTCACTAACATTGGCTGATACACAATCTAAAAAAAT CCAGAAACAGCCTTTACCTGCAAACAAGAAAGGGCTACTCCCAAGCCAGTCTGAAGTCTCAAAAGCTCAG GATGGATCTTCAGGTTTTCTACAGCAGACCCTACCTCTCCAGAACACTTTAAAGTTGCTTCAAGAGCAGC TGACTAGACAGAGGAGTATGATTCCGCCTAGACGTGATGGTCAAGAGACATTACTTTTATATAAAGAAAG CTGTTCAGAAGACAGTGAGGCTGGGCCAGTAGAATCACTCTCCTCCGTGGTTGTTCAGCATGCAGATGCT TCTCGTGCAGTTTCAGAAGTACCTAAGCGGCTCCAGGACGTGTATTCATCTGAGGAAGAAAATAGAGTTC TCTCCAGTCATTTGATCACCCATGGCTTTCCACAGCACAGCCTGCAAAGACAAGAACATTTTACACCACT CCAGGAAGAGACACACATTCAGAGGCTTATACTTGGTGCTAGAAAAAATAATGAGGAATTTGCTCCCAAG CAAAATGAATTAGAAAAGGGGCTCTGCTCTCAACAGACTGATGCCTTGTCTTCTCCATCCCAAGTCACCG ACTGGGGAACATCCCGGGGGTCTGTATCAGTCAGAAGTGATAGGACAGACCCCTTAAGGCATTTTAAGAT TCCAGCGTTTCGGGAAAGACTAGTTAGAGTTTCACAACATACATTCCCTCTCCAAGATAATTTGCAGGAA CACCAAGAATGGGTAGACACAGAAAAAGAGAGCTTTCAATCTAGTCCTCTAACTCCAGAAAATCCATCTT CTCAACAGACAGGTTTTTCGTCCTTCAAAGCCTCATTAAGACTGCCATCGTGTGTGTCCTTGCCATCTGC TGACTCTGGTATCACACAGCACCCTCTCTCAACAGAGAGTGACAGTAAAGTCAAGTCAAGCCATCTTCAG ATACCAGAACTGCAGCATAGACTTTCGAAGATATCCCAGCTCATCCCGCCTCAACAAGACAGTTTGAAGG CCCTTCAAGAACAATTAGCTACACAAAGGGAAGCCATCATCCACTCTAGACAAGAAGCTCATGAAGAAAC ACTAAGAGAATGGAAGGAAAAAATATTCCCAGAGCAGGTTGGTCCATTTTCTCCCCTGATACCACAACAT TCACTTGCTTCATTCCCTGTTTCTGATACTGAAAGAGCACAAGAACTGTGTTCAACCAACAGTGACACCA TATCTTCAGGTTATCCTGAGATGCTGGAGTTGCCTGACAGGACATTGGGTTTATCACATACTGCTTTACC TCAGCAGAATAATCTGACTGCACATCCAGAACACCTTCATGCACAAACAAATTTTTTTCACTCTACTGAG AAAGCCCAAGAAGGGTTGGTGTTTCCCAGACCATGTCAATTTGAAGAGATGTCTGCTGAGCATTTTATCC AGCCTCAACATGATGACTTAAAGGCACTTCAACAGCAGTTAGATATGCAAAGGGAAGCCATTAGATCTGG CCAGGAAATGCAAGAGAAAATGCTTTTGCAAAGGTTAAATAAATTGGAGCAAAGGATATCTTCCAAACAA ATTAGCTCCTCTCTATTTTCATCCCAGGTAGCACTGCCCATTGCCAATTCTGACGGGACCTTACAGTCTT TCCCAACTAAAAGTAACGAGACTGAGCTGCTTGGGTCCCAAGATGAGTATCTGAGCTTTTCACAGCCTCG TCTGCCTCTGCAGAATAACATGACAGAGCAGTTGGACTTAGAAAAGGTATTTCATAAAGAACTGCTTTTA CACAAACAAAAGAGCCAAAACAAAAGTGAATCTTCTGAGCATTCCCTCCCTCCTTTGTTTCTGTCCAAGG AAATAGAGCATCCATTCATTTCACTACCTTTTGCAGAAAGTAAATCTAAAAGCATTTGTGAGTTGTATTT ATCTGACAAGAAGCATGCAGCTCCTAATGATGCTGTGATCCCAAGATTGCAGGACAGGCTTTTAAGCTGT TCCCAACCTGTCTTAACTCAGCAAGATAACATGAGTCTTCAGAAGCAGCTGAATCTACAAAGAGAAACTC TACACTCTAGGCAAAAAGCCCAAGAAGAATTGCTTGTGCAGAGACAGACATCCTTACAGCAGCAAATCCA AAGACACAGAGAGACTTTGAAAAATTTCTTTAATGTCAGTCAGGCAAGGAATCCCACAGATGAGAATGAC TTGGAAATGCAGAAGAGAGAACAGCTCGGAGGGTGGTTTCCTCACACCCAAGGCCTTACCTGGGGAGACG CAGGTCAGGGGAGCGCTAATGGTGAGCAGCCACGTGCAGATGTCCATGCTGAGCACAATGGTGAGAGTCT GGCCAAAGAACTGAGTGGTAGAGCCTCAAAGCCACCTGTCTCCAAAGTTAAATGTGTCTTGGACTTAAAC CAGCATGAGCTTAGTACTATACAGGAAGTAGAGTCACCAGCAAGTGGCAGGATTTCCATGCCAGGAAAAG CAGAGTTTTATCAAGACCGAGACCCATTAAGGGTCTCAGTAAGCAGAGAACAAAGTTTCTTGGAGAGCCC ACTGGCACATGATCCATTTGGTTGTCACCAACCACCTGCCCAAGAGAACAGCAAAAGCCATGACGACAAT GCTGAAGCAGTTAAAGTCAAGAAATCTGATGTTGAGGATCATGCAGTATTGAGTCATGCTGTCTCAAAAG AAGAAGCGTGTACAAACCTGGGTCCACTTGGAAAGCCAGACGATGAGGCTGAAACACAAGAGATTTCTCA GGAGCCATTGTCTTCAGTAACTGTTTCTACTGGAAGCTTCTTAAGTTATGAAATCACAGATTTGAGCCTC ACAGATCCAGTTCCAGCTGTGGACCTTGACTTTCGAGAATTGGAACACATATTTCCACACTTGCATCGTC AGCTCTTTAAACCCTTAGAACCACATCTAGATTTTGACTTATCATCCCCTGGGACATCTCAAGAAGACAG CGACTTTTACCAGAGCTCAGAATCTTCATCTGAAAAACATGTTAAGGCATTATCCACAGGCACAATCTGC TTCCCAGCACTGACAGCCAAGTCACATTCTCCTAACCCAAGGCTGAACCAACTTGATATTAACCTGGCTC ATGCAACAACTGAGGGATCTGAACAGTCTTTTCAACAGCTTCGACCAGAATTCTCTTCACAGGAAAGCCA ACATGCTGACCTACCCAGTATTTATAGCATTGAAGCAAGAGGTCCTTCCCAACGCATGGAAAACCAGAAC TATTCTGAAATGTTACAAAATAAGAAAAAAAGTCTCTCTCTCCAGCCCTCAACAGAGGATTTGACTCCAG CTTGCAGTTCATCTGATACTGCTCTGTTTGATCAGTTACACCTGCAGCACAGCACTCCGTGTGCTTCTGT CTCTAGTGAGTGCTCAGTAAAACTGTTAGAAAGCAGAGAAGAAGTGCTGGGATTTGAAGAGCTGTCAAGA AGGGCAGTTACAATGTCACAAAGACTCACTGAAGATGAAAATGTAGTTCTACCTATAAACCCACATGTAG GAAGAGTGGAGAAGGAGGCTTCAGTTCAGGGGTCTAATCCATTGAGTATTCAAAATGAAAAGCCAATTCA GAACTTCATTGAAACTGACACTACAGAAGCTGTAGGAAATGTATGTCAGCTAGCACAAGCAGAGCACATA TTAAAATCCTGTCCATTTCGGAGCCCTATTCCAATCTGGGAAACGGATACTGGCTATGGTATAATGGAAG AACCAGATCTCACTCTAGTGAGTAATAGTGACATCAGCATCACTGAAACAGATCTTGCAAACTTAACCCT TGAAGACAGAGAAGACAATGAAGCACAGTTTTTTCAGGCAGGTGTCGTTCTGCCTACATCTTCAATGGAA ACCTCTGTTTGTGGAGCTGTATCAGAGCCTTATGTGGACCAACCCACAGTGGCACCTTCAGCTACATCAG GTAGTTTACAAGAAGCATTTATGACAAGACAGACACTTACAGAACGATCTTACCAGAGGCAGAGAGAGAT CTGGAATAAAACTCGACTCCCTCAAACCAAAGTATCCAAAGAGAAGCTACCTACAGGTTGCACAGGCAGT TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_176976
Insert Size	7143 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_176976.4, NP_795950.2
RefSeq Size	7380 bp
RefSeq ORF	7143 bp
Locus ID	319675
UniProt ID	Q8BQ48
Gene Summary	Centriole-enriched microtubule-binding protein involved in centriole biogenesis. Essential for the generation of the distal portion of new-born centrioles in a CENPJ- and CEP120-mediated elongation dependent manner during the cell cycle S/G2 phase after formation of the initiating cartwheel structure. Required for the recruitment of centriolar proteins, such as POC1B, POC5 and CEP135, into the distal portion of centrioles. Also required for centriole-to-centrosome conversion during mitotic progression, but is dispensable for cartwheel removal or centriole disengagement. Binds to and stabilizes centriolar microtubule.[UniProtKB/Swiss-Prot Function]

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