Flg2 (NM_001013804) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	EG229574
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225291 representing NM_001013804 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATATCTCTTAAGAAGTGTTGTCACCATCATTGATGTATTCTACAAATATACCAAGCAAGATGAAG AATGCGGCACACTGAGCAAGGATGAGCTAAAGGAACTGCTGGAGAAGGAGTTCCGGCCAATTCTGAAGAA TCCAGATGATCCAGACACAGTAGATGTCATCATGCATATGTTGGATCGAGATCATGACCGAAGACTAGAC TTTACTGAGTTTATTCTGATGATATTCAAACTGGCTCTGGCCTGCAACAAGGTTCTTGGGAAAGAATACT GTAAAGCTTCAGGGTCAAAGAAGCATAGGCGTGGCCATCAACACCAAGAGGAAGAAAGTGAAACAGAAGA GGAAGAGGAGACACCAAGACAGAAATCAGGTTTCAGATTTTCAAGTTGGAGTGAGGGAGAGGAGCATGGA CATAGTTCTGGGGGCTCACGGGGACCTGCAAAACATAGACGTGGGTCCAACTCTAAGAGGTTGGAAAGGC AAGATGAATTATCCAGCTCAGAGGAATCAAGGAAAAAACACCATGGGTCTATCTTTGGTCACTCTTGGAG TAGTAACAAAGAGAAAGATGGTTCCAGATCTGAAGAGCTAGGAGAAAAAGGAGACAAGTCATATGATAGC CCCTCTAGAGAATCTGAGGAAGAGTATGAGTCTGGATATAGGTTAAACCACCAGGGAAGGGAAGGTCATA GTGGATTGTCATGTGGATTGGAGAAGAACAAATATGAGTTAAATTACATTCAATTAAGAAAGGGTGGAGA ACAAAAGCTTGGGTATAATACTTCAAGTTCAGGAAACAGTAAGATACAAAGCCATGTATATGGTTTTAGC AATTCTAGTGGATGTTGCAGACCAAAAAATGCTTCAAGTTCTTGTCAGGCAAGTAGATCACAGGGACAAG GAAATCAGTCTTGCCGTACCCAGTCAAACTGTCAATCAGGAACTAGTGGAGGACAAGGATATGGGTGTGT CTCAGAAGGTCAGTCCTCTAGATGTTGTCAACCTAAACCTAGATCCTGTAGCCAGTCTTCTAGTCAGAGA GGGTATGGATCTAAACAATGTGGTCAACCACAGAACTGTGGAAGACAACAAAGAATGGGTTCAAGTCACT CTTCTTGCTGTGGACCATATGGGTCTGGAGCAACTCAATCTTCTGGTTGTGGCCAACAGAGAATGAGTTC CTGTGGACATTCTTCAAGCTCTCATCAAAAGGGGTGTAGTTCAAATGGATTTTCCAAAGGTGATCAGCGT GCATCTGGTTCGGGGCACTCATCCTGCTGTGAACAACATGGAACAAACTCAAGTCAGTCCTCTGGCTTTA AACAACATGGACATGAATCAGGTCAGTCTTGCTGTGGCCAACATGGTACTGCATCAAGTCAATCCTCAGG TTATAGTCAACATAGGGTTGGCTCAGGGCAGTCATGTCACTATGGGCAGCATGGGTCCAGTTCTGGTCAG TCATCTAGCTCTGGGCGTCATGGATCTGGCTCAGGTCAGTCATCTAGCTCTAGGCATAACAGGTCAGGCT CAAGTCAGTCATCTGGCCTTGAGGAGCATGGGTCCAGTTCTCATCAGTCACATAGTTCTGGGCATCATGG ATCTGGATCACGTCAGTCATCTGGGTCTGAGCAGCATGGTGCTGTCTCAGGTCAGTCATCTGGCTCTGGG AAGCATGAGACTGGACCCAGTCAGTCATCTAGTTCTGGGCATCATGGTTCTGGCTCTCAGCAGCATGGGG GTGGCTCAGGCCAGTCAACTGGCTTTGGGGAGCATGAGTCGAGTTCTGGGCACTCATCTAGCTCTGGCCA ACACAGGTCAGGCTCCCGTCACTCATCTGGCTCTGGGAAGCATGAGTCTGGACGCAGTCAGTCATCTGGT TCTGGGCATCATGGTTCTGGCTCTCAGCAGCATGGGGGAGGTTCAGGAAACTCAACTGGCTTTGGGGAGC ATGGATCCAGTTCTCATCCACTGCCTAGTTCTGGTCAAAATGAATCTAGCTCAGGTCAGTCTTCCAGGTC TGAGCGGCATGGGACAGGCTCAGGTCAGTCATCTGGCTTTGGGCAACATGGGTCAGGTTCTCATCAGTCA TCTAGCTCTGGGCATAATGAATATGGCTCAGGTCAAACATCTAGCTCTTGGCCGCATGGGAAAGGCTCAG GTCAGGAATCTGGCTATGGAGAGCAGGAATCTGGACATGGTCAGTCTTCTAGCTCATGGCAACATGGGAC TGGCCCAGGTCAGTCATCAAGCAGTGAAGAGGAGGAATCTAGACCTGGTCAGTCTTCTAGCTCATGGCAA CATGGGAAAGGCTCAGGTCAGGAATCTGGCTATGGAGAGCAGGAAGCTGGACATGGTCAGTCTTCTAGCT CATGGCAGCATGGAACTGGTGCTGGCAACCAGTCATCTGGCTATGGAGAGCACAAGTCTGGACCAAGTCA TTCATCTAGATCTTGGCACCATGGGACAGGCTCAGGTCAGTCATTGGGATTTGGACAGCATGGGAAGGGA TCTCATCAGTCAGAAAGTTCTGGACATTATGAATCTGTCTCAGAACCATCCTCTAGTTCATGGCAGCATG GGAATGGGTCAGGTGAGTCTTATGGATACGGAGAGCATGAATCTGGACATGGTCAGTCATCAAGTGCTTG GAATCATGGAAATGAATCAGGTCAGTCTAATGGATATGGAGAGCATGAATCTGGACATGGTCAGTCATCA AGTGCTTGGAATCATGGAAATGAATCAGGTCAGTCTAATGGCTTTGGGGAGAATGAATCAGGACGAGATC AGGAGGGATATCAACAGAGAGAGTCTTTTCATGGCCAACATAGACATCCGCTATCCCAACATGAACAACA CTCACAGTTTGGATATGGTAGATCTCCAAGATCACCAGTACACCCTGAGTCCAGTGAGGGTGAGGAACAC TCAGTAGTCCCACGGAGATATTCTGGATATGGTCACGGTCAGGGTCAGGCAGGACATCAACAGAGAGAGT CAGGGTATGGCCAACGGGGCAGACCTCAAGGGCCCTCTCAGGACAGCAGTAGACAGCCTCAGGCCGGTCA TGGACAGCCCTCACAGTCTGGCTATGGCAGATCTCCAAGGAGATCACAAGTCCACCCTGAGTACAGCGAG GGTGAGGCACACTCAGAAGTCTCACAGAGACATTCTGGATCCAGTCACTGTCACTGTCACTGTCATGGTC AGGCAAGACATCAACAGAGAGAGTCTGTGCATGGCCAACGGGGCAGACCTCAAGGGCCCTCTCAGGACAG CAGTAGACATCCTCAGGCCGGCCCTGGACAGCCCTCACAGTCTGGCTCTCGCAGATCTCCAAGGAGCCAA CCAGTCCACCCTGAGTCCAGTGAGGGTGAGGAACACTCAGTAGTCCCACAGAGACATTCTGGATCCGGTC ACGGTCACGGTCAGGGTCAGGGTCAGGCAGGACACCAACAGAGAGAGTCTGTGCATGGCCAACAGGGCAG ACCTCAAGGGCCCTCTCAGGACAGCAGTAGACAGCCTCAGGCCGGCCAGGGACAGCCCTCACAGTCTGGC TCTGGCAGATCTCCAAGGAGATCACCAGTCCACCCTGAGTCCAGTGAGGGTGAGGAACACTCAGTAGTCC CACAGAGACATTCTGGATCCGGTCACGGTCACGGTCAGGGTCAGGGTCAGGGTCAGGCAGGACATCAACA GAGAGAGTCTGTGCATGGCCAACGGAGCAGACCTCAAGGGCCCTTTCAGGACAGCAGTAGACAGCCTCAG GCCGGCCAGGGACAGCCCTCACAGTCTGGCTCTGGCAGATCTCCAAGGAGATCACCAGTCCACCCTGAGT CCAGTGAGGGTGAGGAACACTCAGTAGTCCCACAGAGACATTCTGGATCCGGTCACGGTCACGGTCAGGG TCAGGGTCAGGCAGGACACCAACAGAGAGAGTCTGTGCATGGCCAACCAGTCAGACCAGAAGTGCCCACT CAGGACAGCAGTAGACAACCTCAGGCCGGCCAGGGACAGCCCTCACAGTCTGGCTCTGGCAGATCTCCAA GGAGATCACCAGTCCACCCTGAGTCCAGTGAGGGTGAGGAACACTCAGTAGTTCCACAGAGAAATTCTGA ATCCTGTCACTGTCACTGTCATGATCAGGCAGGACATCAACAGAGAGAGTCTGTGCATGGCCAACGGGGC AGACCTCAAGGGCCCTCTCAGGACAGCAGTAGACATCCTCAGGCCGGCCCTGGACAGCCCTCACAGTCTG GCTCTCGCAGATCTCCAAGGAGCTCACCAGTCCACCCTGAGTCCAGTGAGGGTGAGGAACACTCAGTAGT CCCACAGAGACATTCTGGATCCGGTCACGGTCACGGTCAGGGTCAGGGTCAGGCAGGACATCAACAGAGA GAGTCTGTGCATGGCCAACGGGGCAGACCTCAAGGACCCACTCAGGACAGCAGTAGACAGCCTCAGGCCG GCCAGGGACAGCCCTCACAGTCTGGCTCTGGCAGATCTCCAAGGAGATCACCAGTCCACCCTGAGTCCAG TGAGGGTGAGGAACACTCAGTAGTCCCACAGAGACATTCTGGATCCGGTCACGGTCACGGTCACGGTCAG GGTCAGGGTCAGGCAGGACACCAACAGAGAGAGTCTGTGCATGGCCAACGGGGCAGACCTCAAGGGCCCT CTCAGGACAGCAGTAGACAGCCTCAGGCCGGCCAGGGACAGCCCTCACAGTCAGGCTCTGGCAGATCTCC AAGGAGATCACCAGTCCACCCTGAGTCCAGTGAGGGTGAGGAACACTCAGTAGTCCCACAGAGATATTCT GGATCCGGTCACGGTCACGGTCAGGGTCAGGCAGGACATCAACAGAGAGAGTCTGTGCATGGCCAACGAG GCAGACCTCAAGGGCCCTCTCAGGACAGCAGTAGACAGCCTCAGGCCGGCCAGGGACAGCCCTCACAGTC TGGCTCTGGCAGATCTCCAAGGAGATCACCAGTCCACCCTGAGTCCAGTGAGGGTGAGGAACACTCAGTA ATCCCACAGAGACATTCTGGATCCGGTCACAGTCACGGTCAGGGTCAGGTTCATGCAGAACATCAACAGA GAGAGTCTGTGCATGGCCAACGGGGCAGACCTCAAGGGCCCTCTCAGGACAGCAGTAGACAGCCTCAGGC CGGCCAGGGACAGCCCTCACTGTCTGGCTCTGGCAGATCTCCTAGGAGATCACCAGTCCACCCTGAGTCC AGTGAGGGTGAGGAACACTCAGTAGTCCCACAGAGACATTCACATTCTGAATCCGGTCACGGTCATGGTC AGGGTCAGGGTCAGGCAGGACACCAACAGAGAGAGTCTGTGCATGGCCAACGGGGCAGACCTCAAGGGCC CTCTCAGGACAGCAGTAGACAGCCTCAGGCCGGCCAGGGACAGCCCTCACAGTCTGGCTCTGGTAGATCT CCAGGGAGATCACCAGTCCACCCTGAGTCCAGTGAGGGTGAGGAACACTCAGTAGTCCCACAGAGACATT CTGAATCCGGTCACGGTCATGGTCAGGGTCAGGGTCAGGCAGGACACCAACAGAGAGAGTCTGTGCATGG GCAACGGGGCAGACCTCAAGGGCCCTCTCAGGACAGCAGTAGACAACCTCAGGCCGGCCAGGGACAGCCC TCACAGTCTGGCTCTGGCAGATCTCCAAGGAGATCACCAGTCCACCCTGAGTCTAGTGAGGGTGAGGAAC ACTCAGTAGTCCCACAGAGACATTCTGGATCCGGTCACGGTCACGGTCAGGGTCAGGGTCAGGCAGGACA TCAACAGAGAGAGTCTGTGCATGGCCAACCGGTCAGACCTCAAGGGCCCTCTCAGGACAGCAGTAGTCAG CCTCAGGCCAGCCAGGGACAGCCCTCACAGTCTGGCTCTGGCAGATCTCCAAGGAGATCACCAGTCCACC CTGAGTCCAGTGAGGGTGAGGAACACTCAGTAGTCCCACAGAGACATTCTGGATCCGGTCACGGTCACGG TCAGGGTCAGGGTCAGGCAGGACATCAACAGAGAGAGTCTTTGCATGGCCAACGGGGCAGATCCCAAAGT CCTTTTCATCCATCACATAGTATCCATTGGCAATCAAAGTGTACCATAAGCAAAAAATCTAGTAGACTTT CTGGCCATTATGGAAGAAATCATTTTCAATCAACCATTAGTGGTAACCAGTATGATTCCAGTCAATCCTC GAGACATGGCAGCTATGGGCCTCAAGACTATGACTATGGTCAGTCTGGGTATGGACCTTCTGGAAGGTTG AGAAGTAACAGCCAGAGTTCTATTCCTTTCAGTTCTGCACATAGAGCAACACATATGGAAGTCTTACCTT GTGGACAGTCTTTCTCTCCTTCTGATCATGTAGGTACCAAAGCAAATGAACAAATTGGAGAGCTAGTTTT TAAATACAGAGAGTCAGAAACAGGTCCTGACCAGTCAGTTGATTATTACAATCTTACTGAGTCCAATAGT ACAACTAGAGGGCATGAGTGTAGTCATGGCCATTCTGTAGTAGTACCTGAACATTCAGATGACAGTGATT TCAATTATGGTCACAGTTATAATGGTAAACAACAAATTTGTCAGAGCCAGCCAACTGTCCAGTCTTGTTT TGATGATAGCCAATACATATTATTTCAGAAACATTTAGAATCCCCTTCATTTGGAAATCAGTCTGGATTC AGCCCTAATGAAAGGCAATTATATACCTGTAACGAATCGATCGATAGCTATCATCTGTCAAGTGATAGCA ACAATAGAAATCAAATATATTCTTCCAATAACTCTTTTCCCAACCTATACTGCATTGGAACTGAACAGTG TATATATTTACCAAGTGCAACCATATTGGGTGAGGGAACAGAAGGACAGGAGCCTGGATATACCCAACCA GGGACCATCTGTAAATATAATCAATTTTTAGATGGCAGGAAGAGTAGAACCAGAGGTAACCATGAAACGG GGAAAATGAAGTCAGGTTCTGCCTACTTAGATAGCAATACTCCGCTATATACATATGTCCAAGAACAGAA ATCCTACTACTTTGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001013804
Insert Size	7089 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001013804.1, NP_001013826.1
RefSeq Size	7755 bp
RefSeq ORF	7089 bp
Locus ID	229574
UniProt ID	Q2VIS4
Gene Summary	Essential for normal cell-cell adhesion in the cornified cell layers. Important for proper integrity and mechanical strength of the stratum corneum of the epidermis.[UniProtKB/Swiss-Prot Function]

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