Myo15 (NM_182698) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Myo15a; sh-2; sh2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225264 representing NM_182698 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCCATACGCAACCTGCCTTCTATGCGGTCCCGTGAGCAGCACAGGGAAGATGGAGTGGAGGACA TGACGCAGCTGGAAGACCTCCAAGAGACCACTGTGCTAGCCAACCTAAAGACCAGATTTGAGCGAAACCT CATCTATACCTATATTGGCAGTATTCTGGTGTCGGTGAACCCATATCGAATGTTTGCAATCTACGGGCCT GAGCAGGTGCAGCAGTACAGTGGGCGTGCCCTAGGAGAAAATCCCCCGCACCTCTTTGCAATTGCTAATC TTGCCTTCGCCAAAATGCTTGATGCCAAACAGAACCAGTGTGTCATCATCAGTGGGGAGAGTGGCTCTGG AAAGACTGAGGCTACGAAACTGATTCTGCGCTGCCTGGCTGCCATGAACCAGAGACGGGACGTCATGCAG CAGATAAAGATCCTGGAGGCAACGCCTCTCCTGGAGGCCTTTGGTAATGCCAAAACTGTCAGGAATGACA ACTCCAGCCGCTTTGGGAAGTTTGTGGAAATCTTTCTAGAAGGGGGTGTGATCTGTGGTGCCATAACCTC TCAGTACCTGCTGGAGAAGTCAAGGATTGTGTTCCAGGCCAAAAATGAAAGAAATTATCATATTTTCTAT GAGCTGCTGGCTGGGCTCCCTGCTCAACTAAGGCAGGCCTTCAGCCTGCAGGAGGCTGAGACCTACTACT ACCTGAACCAGGGCGGGAACTGTGAGATCGCTGGAAAGAGTGACGCAGATGACTTCCGCCGTCTGCTGGC TGCGATGGAGGTGCTTGGCTTCACCAGTGAGGACCAGGACAGCATCTTCCGAATCCTAGCCTCCATCTTA CACCTGGGCAATGTCTATTTTGAGAAACATGAGACCGATGCCCAGGAGGTGGCCTCGGTGGTGAGCGCCC GGGAGATCCAGGCTGTGGCAGAGCTGCTTCAGGTCTCTCCTGAGGGCCTGCAGAAGGCTATCACCTTCAA AGTGACTGAGACAATTCGAGAGAAGATCTTCACACCCCTAACAGTGGAGAGTGCCGTAGATGCCAGGGAC GCCATCGCCAAGGTCTTGTATGCACTGCTGTTTGGCTGGCTCATCACCAGGGTCAACGCTCTGGTGTCCC CAAAACAAGATACATTGTCCATCGCCATCTTGGACATATATGGCTTTGAGGACCTGAGCTTCAACAGCTT TGAACAACTGTGCATCAACTATGCCAACGAGAACCTTCAGTACCTGTTCAACAAGATCGTCTTCCAGGAG GAGCAGGAGGAGTACATCCGGGAGCAGATGGACTGGAGAGAGATTGCCTTTGCTGACAACCAGCCCTGCA TCAACCTCATCTCCCTGAAGCCCTACGGCATCCTGCGCATCCTGGATGACCAGTGCTGCTTTCCCCAGGC CACAGACCACACATTCCTGCAGAAGTGCCACTACCACCATGGCGCCAACCCGCTCTACTCTAAGCCTAAG ATGCCACTGCCAGAGTTTACCATCAAGCACTATGCAGGCAAGGTCACCTACCAGGTGCACAAGTTCCTGG ACAAGAACCATGACCAAGTGCGCCAAGACGTCCTGGACTTGTTTGTGCACAGCCGCACAAGGGTGGTAGC ACACCTTTTCTCCAGCCACGCTGCGCAGACTGCCCCTCCACGCCTGGGCAAGAGCAGCTCCATTACCCGT CTCTACAAGGCTCACACGGTGGCGGCCAAGTTCCAGCAGTCCCTCCTGGACCTGGTGGAAAAGATGGAGA GGTGTAACCCTTTGTTCGTGCGCTGCCTGAAGCCCAACCATAAGAAGGAACCTGGTCTCTTTGAGCCAGA TGTGATGATGGCACAGTTACGCTATTCAGGGGTTCTGGAGACTGTGAGGATCCGCAAGGAAGGCTTTCCG GTGCGGCTACCCTTCCAGGTGTTTATCGACAGGTACCGGTGCCTGGTGGCCCTCAAGCTCAACGTGCCAG CAGATGGGGACATGTGTGTGTCATTGCTGAGCCGGCTATGCACGGTCACTCCAGACATGTACCGTGTTGG GATTAGCAAGCTCTTCCTCAAGGAGCACCTGCACCAGCTACTGGAGAGCATGCGGGAGCGAGTCCAGAAC CGAGCTGCCCTCACCCTGCAGCGCTACCTGCGAGGTTTTTTCATCCAACGGCACTTCCGGTCCTTGCGGC GGAAGATCATCCTACTGCAGAGCAGAGCCCGTGGTTTTCTGGCCAGACAACGCTACCAGCAGATGAGGCA GAGTCTGCTGAAGTTCCGTTCCCTGGTGCACACCTACGTGAACCGCCGCCGCTACCTCAAGGAGCTGAGC AAGCGTGAAGTGGTCCCAGTGAGACATCTGGAGGTGCCAGCTGAGGTGGCTGGGCTCCTGCAAGCCGCAG CAGGCCTCAAGCTGTCCAGTGGGCCCCGAGTGGCTGTTGTCCGGGCTCCTAGGCTCCAGGCTGAGCCGTG TGTCACACTTCCCCTTGACATCAACAACTACCCCATGGCCAAGTTCATTCGATGCCACTTCAAGGAACCC TCCTTTGGGATGCTGACAGTGCCCCTGAAGATGCCTCTCACACGGCTGCCTGTGGAGCACCACGCAGAAG CCATAAGTGTCTTTAAGCTAATCCTGCGTTTCATGGGAGACCCCCACCTGCATGGCACCCAAGAGATGAT CTTGGGGAACTACATTGTGCACCAGGGGCTGGTGGAGCCAGCTCTGAGGGACGAGATCCTAGCCCAGCTG GCCAACCAGGTGTGGCGCAATCCCAATGCCTACAACTCCAAGCGGGGCTGGTTGCTGTTGGCTGCCTGTC TCAGTGGCTTTGCGCCTTCCCCGCACCTTGACAAGTTCCTCCTCAAGTTTGTATCTGATTATGGACAGAA TGGCTTCCAGGCTGTGTGCCAGCATCGCCTCCTGCAGGCGATGGGCTCAGGGGCTGCCCGGACCTTTCCC CCAACTCAGCTGGAGTGGACTGCCATTCAGGAGAAGGCCAGTATGGCTCTAGATGTGAGCTGCTTCAATG GTGACCAGTTCTCCTGTCCGGTCCACACCTGGAGTACGGGGGAAGCGGTGGCTGGAGACATCCTAAAGCA CAGGGGGCTGGCTGATGGTTGGAGGGGCTGGACAGTGGCCATGAAGAATGGAGTCCAGTGGGCAGAGCTG GCTGGACATGACTATGTGCTGGACCTGGTATCAGACCTGGAACTGCTCAGGGACTTCCCACGACAGAAAT CCTACTTCATAGTGGGTGCAGAAGGGCCTTTGGCTGGCAGGGGAGACACCAGAGGAGTGTTTGGGAACTG CTGGGACTCAGACGAGGACACACCCACAAGGCCCCAGCCCCAGGACCACGTGGCCAAGATGCCTGACTTG GATGGGTACTGCAGCCACAAAGAGGATGGTACAAATGGAGAAACTGAGGCCCAGAGATGGACATCGAACC GCCAAGCTGTGGACAGCATTGGAGAGTCCACTGTGCCCCCCAGGGAGCTGGATGGCTACTTGGACAGTCT CTTCGACCCTGTGCTGGCCTGTGGGGATGCGGACCTGGAGAAGCCGACAGCCATCGCCTACCGCATGAAA GGTGGGGGCCAGCCAGGTGGAGGTGGTGGCAGTACTTCTGAAGATACCTCCCGGAGACCCCCGGAACCCA AGCTCAAGCCCATCCCAGGCCTGGATGCCTCCACACTGGCTCTGCAGCAAGCCTTCATCCACAGGCAGGC TGTGCTACTGGCCCGGGAGATGACCCTGCAGGCTTTGGCACTTCAGCAGCAGCCTCTGAGTGCAACCTCA AGACCTCAGCTCCCAGAGAGACCCCTAGCACCAGAGGCACGGCCGAAGACTGTAGTAGGCACTGGCCCCC CAGCCAAGCCTGTGCTGGTGCGTCCGACTCCACAGTCGTGGGCTCCGGGCTCTGTGGCAAAGGCCCCGAA GATACCTAGCAAGCCCGTGGCTGTCCCAATCCTAGCTCAAGATTGGACTGCTCCAGAATCCATTTCGGCT TCCCCTGAGCTGGTTCGGTATTCCACACTCAACTCAGAGCACTTCCCACAACCCACACAGCAGATCAGAA GCATCATCAAGCAATACAAACAGCCACCCTGGGCAGGACACCCTGAGGCCCGCAGGACGGATGGTGGAAA GGTCTTCAGGAGGCCACCTGACCCCCATGAGGAGGCCCTAATGATTCTGAAGGGACAGAAGACTCAACTA GCAGTAGTGCCTGGCACTCAGGTGTCCAGAGAGGCTGTGGCCATGGTGAAGCCGGTGACCAGTGCTCCAA GGCCATGCATGGGGCCCACTCCAGTGCAGCCTTCAAGGTCGCTGGAGCCTCCAGAGGATCCTGTGCAGAC ACAGCTGCACCGCCTAGTCAACCCCAACTTCTATGGCTACCAGGACATCCCCTGGCGGATCTTCCTGCGC AAGGAGGTATTTTACCCCAAGGACAACTACAGTCACCCTGTGCAGCTAGACCTCCTGTTCCGGCAGATCC TGCATGACACATTCTCCGAGGCTTGCCTGCGCATATCTGAGGATGAGCGGCTGCAGATGAAGGCTCTGTT TGCCCAGAACCAGCTGGACACACAGCGACCTCTGGTAACAGAAAGTGTGAAGCGGGCCGCCATCAGCATG GCCCGAGACAGCTGGGAGATCTACTTCTCTCGCCTCTTCCCGGCAATGGGCAGTGTTGGCACCGGCGTGC AGATCCTAGCTGTGTCTCACACTGGCATCAAGCTCCTGCAGATGGTCAAGGGCAGCAAGGAGGCCAGCAG GCGGCTCCGCGTCCTGTGTGCATACAGCTTTGCAGACATCCTGTTTGTAACCATGCCATCTCAGAACATG CTAGAGTTCAATCTGTCCAACGAGAAGCTCATCCTCTTCTCAGCCCGGGCGCAGCAGGTCAAGACCCTAG TAGACACCTTCATTCTGGAACTGAAGAAGGATTCTGATTATGTTGTGGCAGTGAGGAACTTCCTGTCTGA AGACCCCGAGCTGCTGTCCTTCCACAAGGGTGACATCATCCACCTGCAGAGCCTGGAACCAACTAGAGTG GGCTACAGCGCCGGTTGTGTAGTCCGCAAGAAACTGGTGTACCTGGAGGAGCTGAGGCGGAGAGGCCCTG ACTTTGGCTGGAGGTTCGGGGCCGTCCACGGGCGCGTAGGACGTTTTCCTTCAGAGCTCGTGCAGCCCGC AGCTGCTCCAGACTTCTTGCAGCTCCCGGCAGAGCCAGGGCGGGGCCGGGCTGCCGCGGTGGCCGCCGCT GTGGCCTCTGCAGCAGCCGCACAGGAGGTGGGGCGCCGGAGAGAGGGTCCCCCAGTCAGGGCGCGTTCCG CTGACAGCGGAGAGGATTCCATAGCGCTCCCACCGAGCACAATGCTCGAATTTGCCCAGAAGTATTTCCG AGACCCTCGGAGGCGTCCCCGGGATGGCCTCAAGCTGAAGTCTAAGGAGGATCGGGAGTCCAAAACTCTG GAGGACGTGCTTTGCTTCACTAAGGTCCCGATCCAGGAATCCCTCATTGAACTCAGTGACAGCAATCTCA ACAAGATGGCGGTTGACATGTTTGTAGCTGTGATGAGGTTCATGGGAGATGCCCCACTGAAGGGCCAGAG CGAACTGGATGTTCTCTGCACCCTTCTGAAGCTTTGTGGAGACCATGAGGTCATGAGAGATGAATGCTAC TGCCAGATTGTGAAGCAGATCACAGACAACAGCAGCCCTAAGCAGGACAGCTGTCAGCGAGGCTGGAGGC TGCTTTACATCATGGCTGCCTACTATAGTTGCTCTGAGGTCTTCTATCCATACCTCATTCGCTTCCTCCA ACATGTGAGCTGGACTCCTGGCCTGCCCTTCCAAGGGATTGCCAAGGCCTGTGAACAGAACCTGCAGAAG ACCTTGCGTTTTGGAGGCCGCCTGGAGTTCCCCAGCAATATGGAGCTCCGTGCTATGTTGGCAGGCCGCA GTTCCAAGAGGCAGCTCTTTCTTCTTCCAGGAGGCCTTGAACGCCATCTGAAAATCAAAACGTGCACGGT GGCCCTGGATGTCATAGAGGGGCTCTGTACCGAGATGGCCCTGACACGCCCTGAAGCCTTTGATGAGTAT GTCATTTTTGTGGTCACCAACCGAGGCCAGCACGTGTGTCCACTGAGCTGCCGGGCCTACATACTGGATG TGGCCTCAGAGATGGAGCAGGTGGACGGGGGCTACACACTCTGGTTCCGGCGGGTGCTTTGGGATCAGCC ACTGAAGTTTGAGAATGAGCTGTATGTGACCATGCACTACAATCAGGTTCTGCCTGACTACCTGAAGGGC CTCTTTAGCAGTGTGCCGGCCAGGCAGCCCACAGAGCAACAACTACAGCAGGTGTCCAAGTTGGCTTCAC TGCAGCACCGAGCCAAGGACCACTTCTACCTGCCCAGTGTGCGTGAAGTCCAAGAGTACATCCCAGCCCA GCTCTACCACACCACGGCCGGTGACACCTGGCTCAACCTGGTTAGCCAACACAGGCAGCAGACTCAGGCG CTCAGTCCCCACCAGGCCCGCGCTCAGTTCCTGGGCCTCCTCAGTGCCTTCCCCTTGTTTGGCTCCTCCT TCTTCTTCATCCAGAGCTGCAGCAATGTCTTGGTGCCAGCCCCCTGTATCCTCGCTGTCAACCATAACGG CCTCAACTTCCTCAGCACCAAGACCCATGAGTTGATTGTGAAGATTCCCCTGAAGGAGATCCAGTCCACG TGGACTCAGCAGCCCACAGCCAATTCCAGTTACCCGTATGTGGAGATCTCCCTGGGGGATGTGGCAGCCC AGCGGACCATGCAGCTCCAGCTGGAGCAGGGTCTGGAGCTGTGTCGCGTGGTGGCTGTGCATGTGGAGTC CATGCTCAGCGCCCGGGAGGAGCGGCTCACACTGCCTCCCAGCGAGATCACCCTCTTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_182698
Insert Size	6921 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_182698.2, NP_874357.2
RefSeq Size	7953 bp
RefSeq ORF	6921 bp
Locus ID	17910
UniProt ID	Q9QZZ4
Gene Summary	Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments (By similarity). Required for the arrangement of stereocilia in mature hair bundles.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two in-frame exons in the 5' coding region, compared to variant 1, resulting in a protein (isoform 2a) with a shorter N-terminus, compared to isoform 1.

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