Ptprq (NM_001081432) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225263 representing NM_001081432 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTTCTTTTCTTTTTTCTTTTCTCTCTCATTGGAACTTCAGAGTCACAGGTTGATGTTTCTGGTT CTTTTGATGACACTGTGTATGATATCACGCTGTCTTCAATTTCTGCCACAACCTACAGCTCACCCGTGAG TAGAACACTGGCAACAAATGTGTCAAAACCGGGGCCTCCAGTCTTCCTAGCCGGGGAGAGAGTTGGCTCT GCCGGGATTCTCCTTTCATGGAATACCCCTCCTAACCCAAATGGAAGAATCATATCGTATGTTGTTAAAT ACAAGGAAGTGTGTCCATGGATGCAGACAGCGTATACCCGAGTGAGAGCGAAGCCCGACAGTCTGGAGGT CCTTCTCACTAACCTTAACCCTGGAACAACATACGAGATTAAGGTTGCTGCCGAAAACAGCGCTGGCATT GGAGTGTTTAGTGACCCATTTCTTTTTCAAACCGCAGAAAGTGCTCCAGGGAAAGTGGTTAACCTCACAG TGGAGGCCCTCAACTATTCTGCAGTAAACTTGATTTGGTATTTACCTCGGCAACCAAATGGTAAAATCAC CAGCTTCAAGATCAGTGTCAAACACGCCAGAAGCGGTATAGTAGTGAAGGATGTCTCCATCAAAGTAGAG GACCTTTTATCTGGGAAATTACCTGAATGTAATGAGAACAGTGACTCTTTTCTGTGGAGTACCACCAGCC CCTCTCCAACCCTCAGTAGAGCCACACCTCCACTTCGTACTACACATTTATCCAACACATTGGCACGGAA TAAGATCAGCTCTGTGTGGAAAGAGCCCATCAGCTTTGTGGTGACTCACTTGAGACCATACACAACGTAT CTCTTTGAAGTTTCAGCCGTTACGACGGAAGCAGGGTATATTGACAGTACCATTGTTAGGACACCAGAAT CAGTGCCTGAAGGACCACCCCAGAACTGTATAACAGGCAATGTCACAGGAAAGGCGTTTTCAATTTCATG GGACCCACCAGCAATTGTCACAGGAAAGTTTAGTTACAGAGTTGAATTATATGGGCCAACAGGAAGGATT TTGGATAATAGTACAAAAGACCTCCGGTTTGTGTTTACGCATCTCACGCCATTCACAATGTATGATGTCT ATGTTGCTGCAGAAACCAGTGCTGGGGTTGGACCCAAGTCAAATCTTTCAGTATTCACTCCCCCAGATGT TCCAGGAGCAGTGTTTGATTTACAGATCGTGGAGGTAGAAGCCACAGAAATAAGAGTTTCTTGGAGGAAA CCTCGACAACCAAATGGAATCATCAGCCAGTACCGTGTAAAAGTGTCAGTCCTAGAGTCAGGAGTCATTT TGGAAAACACTCTGCTCACAGGACAAGATGAGTATATAAACAACCCCATGACTCCAGAAATAATGAACCT AGTAGACCCAATGATAGGATTCTATGAGGGCTCAGGAGAGATGTCTTCAGACCTGCACTCCCTGGCTTCG TTCATATACAACAGCCATCCACACGACTTTCCTGCCAGGACAAGAGTTGAAGACCAAAGATCACCAGTTG TGGCCACCAGGAATCAGTATATGACTGACATTGCTGCTGAACACCTGTCTTATGTTATCAGGCGGCTTGT ACCCTTCACTGAGCACACGATTAGCGTATCTGCTTTCACTGTCATGGGCGAAGGACCACCAACAGTTCTC ACTGTTAGGACACGTGAGCAAGTGCCAAGCTCCATCCAAATTATAAACTATAAAAATATTAGTTCTTCAT CTATTTTGCTATATTGGGATCCTCCAGAATATCCCAATGGGAAAATCACTCATTATACAATCTATGCAAT GGAACTGGATACAAACAGAGCCTTCCAGATGACTACTGTAGATAACAGCTTTCTCATCACAGGGTTGAAG AAATATACAAGATATAAAATGAGGGTTGCTGCCTCAACTCATGTTGGGGAAAGTTCTCTGTCAGAAGAAA ATGACCTCTTTGTGAGAACTCCAGAAGATGAGCCAGAATCATCACCTCAAGATGTCAAAGTAACTGATGT GTCTCCAAGTGAACTGAGCTTGACCTGGTCCCCACCTGAGAAACCTAATGGGATCATTATTGCCTACGAG GTGTTTTACCAAAATGCAGATGCCTTGTTTGTAAAGAACACCTCCACCACGAACATAACTTTAAGTGACC TGAAACCTTACACACTCTATAATATTTCTATCCAGTCATACACCAGACTTGGTCATGGCAATCAGTCATC CTCATTACTTTCTGTAAGGACTTCAGAGACTGTACCTGATAGTGCGCCAGAAAATATTACTTACAAAAAC ATTTCCTCGGAAGAGATTGAGATTTTCTTCCTTCCTCCAAGGAGTCCCAATGGCATCATACAAAAGTACA CGATTTACCTCAAGAGAAGTAATAGCCACGAGGCGAGAACTATAGAAACAACCTCTCTCACTCTCACTAT CGGAGGACTGAAGAAGTATACCCATTATGTGATTGAGGTGTCTGCCAGTACACTGAAAGGTGAAGGAGTT CGGAGTATGCCAATAAGCATACTGACTGAGGAAGACGCTCCTGACTCTCCTCCTCAGAACTTCTCCGTGA AACAGTTGTCTGGTGTCACCGTGATGTTGTCATGGCAACCACCCTTGGAGCCAAATGGAATTATCCTCTA TTACACAGTTTATGTCTGGGATAAAGTATCATTAAAAACTATTAATGCAACTGAAGTATCACTGGAGTTA TCCGACTTAGACTATCATGCTGACTATAGCGCCTATGTAACAGCTAGCACTAGATTTGGTGATGGGAAAA CAAGAAGCAGCGTCATTAACTTCCGAACACCAGAGGGAGAGCCCAGTGATCCTCCCAAAGATGTCCACTA TGTGAACCTCAGTTCTTCATCGATAATTCTCTTTTGGACTCCTCCTGTAAAACCTAATGGAATCATACAA TATTATTCTGTTTATTACCAAAATACTTCAAGTACTTTTGTGCAGAATTTTACACTTCTCGAAGTAACCC AGGAGCCTGGCAATGTGACAGTGTCTGCAAGAATCTATAAACTGGCAGTATTCAGCTACTATACATTTTG GCTAACAGCAAGTACTTTAGTTGGAAATGGGAATAAAAGCAGTGATGTAATTCATGTCTACACAGACCAA GACATACCTGAAGGTGGTGTTGGAAACCTGACTTACGAGTCCCTTTCATCAACTGCAATAAACGTGAGCT GGACGCCACCATCTCAACCAAATGGCCTCGTCTTCTACTACGTTTCCCTGAATTTACAGCAGAGCCCCCC GCGGCACAGGAGACCACCGCTGACTACATATGAAAACAGCATATATTTTGATAATCTGGAAAAATATACT GACTATATATTTAAAATCACTCCATCAACAGAAAAGGGATTCTCTGAGACCTATACTGCCCAGCTACACA TCAAAACTGAAGAAGATGTTCCAGACACTCCACCAATAATCAACACTTTTAAAAATCTTTCTTCTACCTC CATTCTCTTATCGTGGGATCCCCCATTAAAGCCAAACGGTGCAATCCTAAGTTACCATTTAACTTTACAA GGAACCCACGCCAACCGCACTTTCGTCACTTCCGGTAACCACATAGTACTGGAAGAACTCTCGCCATTTA CTTTATATAGTTTTTTGGCTGCGGCAAGAACTATGAAAGGACTTGGTCCTTCCAGTATTCTTTTCTTTTA CACAGATGAGTCAGCGCCTTTAGCACCTCCCCAGAATTTGACTTTAATCAACTACACTTCAGATTTCGTA TGGCTGACGTGGAGTCCAAGCCCTCTTCCGGGCGGTATTGTTAAAGTGTATAGTTTTAAAATTCATGAGC ATGAGACTGATACTGTATTTTATAAGAATATTTCAGGTTTTCAGACTGATGCCAAACTTGCTGGACTGGA GCCTGTGAGCACCTACTCAATTAGTGTGTCTGCCTTTACCAAAGTTGGAAATGGCAATCAGTTCAGTAAT GTGGTGAAATTTACAACCCAGGAGTCTGTTCCAGATGCTGTACAGAATATTGCATGTGTGGCACGTGACT GGCAGTCAGTATCAGTGATGTGGGACCCACCAAGAAAAGCAAATGGAATAATTATTCACTATATGATAAC AGTTGAAGGGAATTCTACCAAAGTCTCCCCCAGAGACCCCATGTACACTTTCACAAAACTTCTTGCCAAT ACATCATACATCTTTGAAGTAAGAGCGTCAACCTCAGCTGGTGAAGGCAATGAAAGCCAATGTAATGTCA GCACACTCCCTGAGACAGTTCCCAGTGTTCCCACGAATACAGCATTTTCTAATGTCCAGTCAACTAGTGT AACACTAAGATGGATAAAACCTGATACTATCCTTGGATACTTCCAAAATTACAAGATAACTACTCAGCTT CGTGCTCAGAAGTGCAGAGAATGGGAGCCTGAGGAATGTGTTGAACATCAGGAAGTTCAATACCTCTATG AAGCAAACCAAACAGAAGACACCGTGCGTGGATTAAAGAAGTTTCAATGGTACAGATTCCAAGTGGCTGC TAGCACCAACGCTGGGTACGGCAATGCCTCCAGCTGGATTTCCACACAAACGCTGCCTGGCCCTCCCGAC GGTCCTCCTGAAAATGTTCGCGTGGTTGCGACATCCCCCTTTGGTATCAATATCAGCTGGAATGAACCTG CCATCATAACTGGACCAACCTTCTACCTGATTGATGTCAAATCGGTAGATAATGATAACTTTAATATTTC CTTCGTGAAGTCAAATGAAGAAAACAAAACCACAGAGATTAATGACTTAGAAGTATTCACAAGGTATTCT GTGGTGATCACCGCTTTTGTTGGAAATGTTAGTGGGGCATACACAGATGGGAAGTCAAGTGCAGAAGTGA TCATTACGACTTTAGAATCAGTGCCCAAGGATCCACCTAATAATATGACATTTCAGAAGATACCGGATGA AGTAACCAAGTTTCAGTTATCATTCCTCCCGCCTTCTCAGCCCAACGGCAATATCCAAGTGTATCAAGCA TTGGTTTACCGAGAAGACGATCCTACTGCCGTCCAGATTCATAACCTCAGCATTATCCAGAAGACAGACA CGTCCGTCATTGCAATGCTCGAAGGCCTGAAGGGTGGACATACATACAACATCAGCGTTTATGCTATCAA CAGTGCTGGCGCCGGCCCAAAAGTGCAGATGCGAATAACCATGGATATTAAAGCTCCAGCACGACCAAAA ACCAAGCCAATTCCTATTCATGATGCCACAGGAAAACTGCTTGTGACGTCAACAACAATTACAATCAGAA TGCCAATCTGCTACTACAATGATGACCACGGGCCAATCAGAAATGTGCAAGTCCTTGTGGCAGAAGCAGG AGCTCAGCAAGACGGAAATGTGACAAAGTGGTATGATGCATATTTTAACAAAGCAAGGCCATATTTTACA AACGAAGGATTCCCTAATCCCCCGTGTATAGAGGGAAAGACTAAGTTCAGCGGTAATGAAGAAATCTATG TGATAGGTGCTGATAATGCCTGCATGATCCCTGGAAATGAGGAGAAAATTTGCAATGGACCTCTGAAACC CAAAAAGCAGTATTTGTTTAAATTTAGAGCCACAAATGTCATGGGACAATTTACTGACTCTGAGTACTCT GACCCTATTAAAACTTTAGGTGAAGGACTTTCAGAAAGAACCGTGGAGATCATACTGTCAGTCACTTTGT GTATCCTCTCAATCATCCTCCTTGGAACAGCTATTTTTGCATTTGCAAGAATCAGACAGAAGCAGAAGGA AGGTGGTACGTACTCTCCTCGGGACGCAGAGATTATTGACACTAAATTCAAGCTGGATCAGCTCATCACG GTGGCAGACTTGGAGCTGAAGGACGAGAGATTAACGCGGTTACTTAGTTATAGAAAATCCATCAAGCCGG TAAGCAAGAAATCCTTCCTGCAACATGTTGAAGAGCTTTGCACAAACAACAACCTAAAGTTTCAAGAGGA ATTTTCGGAATTACCAAAATTTCTTCAAGATCTCTCCTCAACTGATGCTGATCTCCCTTGGAACAGAGCA AAAAATCGCTTTCCCAACATAAAACCATACAATAATAACAGAGTGAAGCTGATAGCTGATGTAAGCATCC CGGGATCAGATTACATTAATGCCAGCTATGTTTCTGGATACTTATGTCCCAATGAGTTTATTGCCACCCA AGGCCCATTACCGGGAACAGTTGGAGACTTTTGGAGAATGGTGTGGGAAACCAGAGCGAAAACATTGGTA ATGCTCACACAGTGTTTTGAGAAAGGACGGATCAGATGCCATCAGTATTGGCCCGAGGACAACAAGCCAG TGACAGTCTTTGGAGACATACTGATTACAAAGCTTATGGAGGACATTCAGATCGACTGGACCATCAGGGA TCTGAAAATTGAAAGGCATGGAGACTGCATGACTGTCAGGCAGTGTAACTTCACGGGTTGGCCTGAGCAT GGGGTTCCTGAAAACACCACCCCTCTAATTCACTTTGTGAAGTTGGTACGAACAAGCAGAGCACACGACG CTACCCCTATGGTTGTGCATTGCAGCGCTGGGGTTGGAAGGACGGGAGTTTTCATTGCTCTGGACCACTT AACACAGCACATACATGACCATGACTTTGTGGACATATATGGACTAGTAGCTGAACTGAGGAGTGAGAGA ATGTGCATGGTGCAGAACCTGGCACAGTATATCTTCTTACACCAGTGCATTCTGGATCTTTTATCAAATA AAGGAGGACATCAGCCGGTCTGTTTTGTTAACTACTCCACACTTCAGAAGATGGACTCTCTGGATGCCAT GGAAGGTGATGTGGAACTTGAATGGGAAGAAACTACCATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081432
Insert Size	6903 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081432.1, NP_001074901.1
RefSeq Size	7058 bp
RefSeq ORF	6903 bp
Locus ID	237523
UniProt ID	P0C5E4
Gene Summary	Phosphatidylinositol phosphatase required for auditory function. May act by regulating the level of phosphatidylinositol 4,5-bisphosphate (PIP2) level in the basal region of hair bundles. Can dephosphorylate a broad range of phosphatidylinositol phosphates, including phosphatidylinositol 3,4,5-trisphosphate and most phosphatidylinositol monophosphates and diphosphates. Phosphate can be hydrolyzed from the D3 and D5 positions in the inositol ring. Has low tyrosine-protein phosphatase activity; however, the relevance of such activity in vivo is unclear. Plays an important role in adipogenesis of mesenchymal stem cells (MSCs). Regulates the phosphorylation state of AKT1 by suppressing the phosphatidylinositol 3,4,5-trisphosphate (PIP3) level in MSCs and preadipocyte cells (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.