Dido1 (NM_175551) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6720461J16Rik; C130092D22Rik; D130048F08Rik; Datf; DATF-1; Datf1; di; dido; DIO; DIO-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225247 representing NM_175551 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGATAAAGGGCACCTGAGCAATGAGGAAGCACCCAAGGCTATCAAACCCACCAGTAAGGAGTTCA GGAAAACCTGGGGTTTTCGAAGAACCACGATTGCCAAACGTGAGGGTGCAGGAGACACGGAGGTGGACCC CAGTGAGCAGCAACCACAGCAGCATAACCTCTCCCTGCGCCGCAGTGGACGGCAACCAAAACGTACTGAG AGGGTAGAAGAGTTTCTTACCACGGTTCGGCGCCGAGGGAAAAAGAATGTGCCGGTGTCCCTGGAGGATT CCAGTGAGCCCACATCTTCCACAGTCACTGATGTGGAGACAGCTTCCGAGGGGAGCGTTGAAAGCAGTTC TGAGATCAGAAGTGGCCCTGTATCTGACTCCTTAGGGAAAGAACATCCTGCCTCTTCTGAAAAGGCAAAA GGAGGTGAAGAGGAAGAAGACACCTCTGACAGTGACAGTGATGGCCTTACGTTGAAGGAACTTCAGAACC GCCTTCGGAGAAAGCGAGAGCAAGAACCTGTGGAGAGGTCCCTGAGAGGCAGTCAGAATCGCCTGAGGAA GAAGCGCAGAGAGGAAGATTCTGCCGAAACTGGGAGTGTCCAAATAGGCAGTGCCGAGCAGGACAGACCT CTCTGTAAGCAGGAGCCTGAGGCTAGTCAGGGACCAGTGTCCCAGTCAGAGACAGATGACATAGAAAATC AGTTGGAAGGGAAGGCGACTCAGGGAAATACAGAGGAAAACCCCAGGGAAGCGGGCAAACCAAAGCCTGA GTGTGAGGTTTACGACCCCAATGCCCTGTACTGCATCTGCCGCCAGCCTCACAACAACAGGTTTATGATC TGCTGTGATCGGTGTGAGGAGTGGTTCCATGGTGACTGTGTGGGTATTTCTGAGGCCCGAGGGCGGCTCC TGGAAAGGAACGGGGAAGACTACATCTGCCCAAATTGCACCATTTTGCAAGTGCAGGATGAGACAAACGG TAGCGCCACCGATGAGCAGGACTCTGGGTGCAGATCTGTGGGTGCTGATGGCACAGACTGCACAAGCATA GGGACAGTAGAGCAGAAGTCCGGAGAAGACCAGGGCATAAAGGGTAGGATTGAGAAGGCAGCAAACCCCA GCGGCAAGAAAAAACTCAAGATATTCCAGCCTGTCGTAGAGGCTCCTGGTGCTCCTAAATGCATTGGCCC TGGGTGTTCCAGTGTAGCACAGCCTGACTCTGTGTATTGCAGTAATGACTGCATTCTCAAACACGCAGCA GCTACCATGAGATTTCTAAGTTCAGGTAAAGAACAAAAAACAAAACCCAAGGAAAAGGTCAAGACGAAGC CAGAAAAGTTCAGTCTTCCAAAATGCAGTGTTCAGGTGGGGATTAAAATCTCTTCTGTGCACAAGAGACT AGCGTCAGAGAAAAGGGAAAACCCAGTGAAGAAAGTGATGCTGGCTTCCAGGAGTGAGACTTCTGGGAAG GAGGCAGCCTGTGAGAGCAGCACACCATCCTGGGCAAGTGACCACAACTACAATGCTGTGAAGCCAGAGA AGCCAGAGAAGCCCACTGCACTCTCGCCCACCCTATTGAGTAAATCCATGAAGGATGACAGGAGGGTGGA GGACAGGACAATGGCAGCAGTTACCATCCCGAAGAAAGCACTTCCTTCAGCCTCTTTGGTGGGCAGACAG ACTTCACCTAGGAATCTTGTTCCAAAGAAGCTTCCTCCTTACTCTAATATGGCAGGAGCCAAACCAGCCA TTAAGAAACTGCCTTCAGGCTTCAAGGGTACCATCCCCAAGAGGCCATGGCCCTCAGCCACCCTGTCAGG CACTTCTGCCAGGCAGGCAGGACCAACACCTATGACAGCTGCTTCCAAAAAGTTGCCTGGCTCTGCTGCT GTGGTGGGAGTTACCAGGAAGCCAATGTCTGCCAATGTTCCTGCTGCTTCTCCAGCCCCAGGACGGCTTG GTCCTGTGAGCCCAGCTCCATCACAGCCCAATTCACAAATTCGACAAAATATAAGGCGCTCCTTGAAAGA GATTTTGTGGAAAAGAGTCAATGACAGCGATGACTTAATAATGACAGAAAATGAAGTAGGAAAAATTGCC CTCCACATTGAGAAGGAGATGTTTAACTTGTTCCAGGTTACTGATAATCGCTATAAGAGCAAATATCGCA GCATCATGTTCAACCTTAAGGATCCTAAGAATCAGGGGCTCTTCCATCGTGTTCTTCGAGAAGAAATCTC CTTGGCAAAACTTGTCAGAATGAAGCCTGAAGAACTTGTATCTAAAGAGCTTTCTATGTGGACAGAGAAG CCCACAAAATCTGTGATAGAATCCAGGACTAAGTTGCTTAATGAAAGCAAGAAGAACACTACTAAACCAG AAACCATTCCTGACATGGAAGATTCTCCACCGGTATCAGATTCAGAAGAACAACAAGAGTCAGTGCGAGC CGCCCCTGAGAAGAGCGCAGCACCTCTCCTGGACGTCTTCAGCAGCATGCTGAAGGACACCACAAGCCAG CACCGTGCCCATCTTTTTGATTTAAACTGTAAAATCTGTACAGGTCAGGTTCCATCCTCGGAGGATGAAC CAGCTCCTAAGAAGCAAAAGCTTTCAGCTTCTTCTAAGAAGGAAGACTTTAAGCCCAGGCATGACAGCTC TCCACCTAATGCAGTTCCTAACACTGCTGATGAAGGGATTGCAGACACGCTGCCTGAAAATGCCTCTGAG CCAGACCCGGAGAGTACATCTAGTCTTAACCAGGAGAGAAAGTGTTTCCCTGAGTCTCCAGGCGATAGCC ATCCTGAGCCCTCGTCTCTGGGTGGCCTTTCTCCCTCTTCTGCCTCTGGTGGGAGTGGGGTGGTCACCAC AGTCACCATGTCTGGTCGAGACCCCAGAACTGCCCTGAGTGGGTCGTGCACAGTCACAGCCTCCATGGCA GCCCATCTGGACAACTCCCAGGCTTCAGAAACCAAACTGGACATGATAAAGCCTGCATTGACTTCTGCAG TGGTGCCCAAGTCCATACTGGCTAAGCCATCCTCCTCTCCTGACCCGAGATACCTGTCAGTACCACCATC ACCAAGCATTTCAGAATCACGATCCCCACCAGAAGGAGATACAACCCTCTTTTTGTCTCGACTCAACACG ATTTGGAAAGGATTTATTAATATGCAGAGTGTAGCAAAATTTGTCACTAAGGCATATCCTGTCTCTGGGT GTTTGGATTATCTCAGTGAGGATTTGCCAGACACCATCCACATCGGTGGAAGGATTGCTCCAAAGACCGT GTGGGATTACGTTGGCAAACTCAAGTCATCGGTGTCCAAGGAGCTCTGTCTGATCCGCTTCCACCCTGCC ACTGAGGAGGAGGAGGTTGCATACATTTCTCTCTACTCCTATTTTAGCAGCCGTGGTCGCTTTGGGGTTG TAGCTAATAACAACAGGCATGTCAAGGACCTGTACCTGATTCCACTGAGTGCTAAGGACCCTGTGCCATC CAAACTCTTGCCCTTTGAGGGCCCAGGTCTTGAATCACCACGTCCAAATATAATACTTGGGTTAGTAATA TGTCAGAAAGTGAAACGTCCTTCAAGTGCTGGCGAGTTAGACAAGACAGATGAGAAGCGGACTCGACTCC AACAGGAAGAACTAGAAACTTCAGTCTATCCCAAAGTAACTGCAGCTTTGCCATCCGAGAAGAAGCCTCC CAAGTACAGTGTACACTCTATAGACACAGCTGCTACCAGCACCACGCCCCCTGGCTCTCCTCCCCCACCT CCTCCTCTTCCTGAACCCCCAGTATTGAAAATACTGTCATCCCTCAAGCCAGGGTCCACCAGCACTGTCA CAGCGCCTACCACAGCAGCAATCACTACCACAGCTTCCCCTGTTACTGCTGCCACTTCCAAAACAGCCTC GCCCCTGGAACACATCCTGCAGACTCTCTTTGGGAAAAAGAAATCATTTGAGCCGTCTGGAAAAGAATCT GTTGGGTCTACTCTATCTCCACATCAGGATTCCAAGGCCAAGGGGGAGGACACCATGTCAGCAGCTCCTT TGTTGGATCCAATTGTTCAACAGTTTGGTCAGTTCTCAAAAGACAAGGCTCTGGAGGAGGAGGAGGAAGA TGACAGGCCCTACGACCCTGAAGAAGAGTACAACCCTGATCGAGCATTTCATACACTGCTCGCTGAGCCA GGGAGGCCTCATGATGTGCAGAGTGTGTCTGAGACAGCTGAGAGGGAGGAGGTGGCCTATGACCCAGAAG ATGAGACCATCTTAGAAGAAGCCAAAGTGACCATTGATGACTTGCCCAATCGCATGTGTATGAAAGTTAG CGCCACAGAAAGGCCTGCCGACTTCACCACCGATGCCTCCAGTGCTTCGCTGGTAGAGCAGCAGAAAATG TTAGAAGAGCTAAATAAACAGATTGAGGAGCAAAAGAGGCAGCTGGAGGAGCAAGAAGAAGCTCTCCGAC AGCAGAGGGCTGCTGTGGGTGTGTCCATGGCACACTTTTCTGTGTCCGATGCATTGATGTCACCACCTCC CAAGTCGTCTTTGGGCAAGACAGAGCTGTTCTCTCAAGAACAGCAAGCCCCAGACCCAAGTCAGGGAGCC CCGAACACCAACCACAACCTAGACTCAAGACAAAGTAGGGACCCAAGGCAGGCCAGGCGGCTCGCTGCAG AAAACACAGAGAATGAATCCCTTCCTAGGGCCCCCACAGGAAGCACACCTGGCCCACAAGGCACCCTACC TGCAAGGGAGACTCCTGCTGGGACTGCAGTAGTCCAGGGGCCTGGGCTGGCAGCAGAAGCCAAGGAGTCA ATGGCTGTTCCCTGGGCTCCAGGTGAAAACGCTGTGTTGAGACCTGAACATGACATCCAAAAATGTGAGC ACCCTGGTAACCCTGTCTCATTACCCCTGGACACCAGCCACCTTCCTACAGCTGGGGATGGCGCAGCCAG GCCAGCACCGCCCCGCAGAGTGCTGCTACCCACACCCCCTAGCACCACCTTTCCACCCAGCTTCCCTTTG CAACCTAAGGCCCAAAATTTTAGTTCTGGAAGCAGGGAGCCTTTCTCAGGGCCCACATTTATGTCTCAGG AAACATCTCTTGGCTCATCCCAATATGAGGACCCCAGGGGTGCTCAGTCTGCTGGGAAGAATGACAGCCC AGTAGCTGATATGGAGGACAGCAGAGAGCCACAGCCTAGGCCTGGTGAGAGTACTACTTCATTCCCCCAA CCTGGGCAGAGGGGAGGGGGCCCTCAGCCTCAGTTTCCTGGCCAGCGTGAACCTGCACCACGCACTTTTG GGATGTCGGGTCATCATGGTCCCAGTTTTCCAGGACCTAGGGGGCCAGTCCCTCCTTATTCAGAAGAGAA CCTTGTTCCTAATAGTGATGGACCAAGAGGCCCTCCACCTGCCAGATTTGGGGCACAGAAGCCACCAATC CCTTCCTTATTCTCTGGGCAACATGGGCCACCTCCCTATGGGGACAACAGGGGACTCTCACCTTCTTATC TTGGTGGGCCCCGAGGAGGAGCACCAGCCCAATTTGAAGACCGCAAGGATCCTCATGGGGAGAAAAGGGA ATTCCAGGACACTCCGTACAACGAGATGACAGGTGCCCCTGCTCAGTGTGAAGGGCCAGATCAGGCCCAG TTCATGGGGAACAGGGCACCCTTTCAGTTTGGAGGCCAGAGACGGCCACTGCTAACTCAAATGAAAGGGC CCCGTGGAGGACCTCCACCCTCTCAGTTTGGAGCCCAAAGGGGACCACCTCCTGGTCATTTCGTGGGCCC ACGTGGGCCCCATCCTAGTCAGTTTGAAAATTCCCGGGGCACTCACCCTGGCCAGTTTGAAGGGGCCAGG GGCCAAGCACCTGGTTTCATGCCAGGCCCCCGGGGTATTCAGCCCCAGCAGTTCGAAGAGCAGAGAGTGA ACTCGCCACCAAGGTTTGCCGGCCAGAGGGCATCGGCGCCCCTGCCATATGGAGGACCAAGGGGCCCTGC ACCCTTTCCTGAAAAAAATGAGCAGCCACCTTCTCGATTTCACTTTCAAGGCCCATCTTCACAGCCCGTG AAGCCTCCCCCGAGGCCCCTGTTGGAGCTACCAAGCCATCCTCCTCAGCATAGGAAGGACCGATGGGATG AGGCAGGCCCAGCCACTGCTCTGCCTTCCAGTGCTGGGCCTGGACAGGGCCATGAGGCCGACGGGCAGTG GGCCACATCAGAGTTCCGAGAAGGCAAAGGCCATGAATATAGGAGCCCAGCTTTTGAAGGGAGACAGAGG GAGCGATTTGAAGCAGGATCTAAAGAAAAGCCTCTGGACGAGCCTGAAGCCCAAGGGTTGGAAAGCCGGC AGGGCCGGGCATTTGAGGATAGGAGGCGAGAGCGAGAGAGAGGCAGAAACTGGAGTCGAGAAAGAGACTG GGAGAGGAGCCGGGACTGGGACCGGCATCGGGAGTGGGATAAAGGCAGGGATAGGAGCTCCAACAGGGAC AGGGAGCGAGACAACGACAGGGCTAAGGAATGGGACCGGAGCCGCGAGAGAAGCCGGAATCGCGACCGGG ACCGAGAAAGGCGGCGAGACCGAGACAGGTCCCGCAGCAGAGACCGAGACAGGGACCGTGAGAGAGCACG AGACAGGGACAGGGACCGAGGCCGGGACCGGAAGGACCGCAGCAAGAGCAGAGAGAGCCCTCGGGACCAG AAGCCTGAGGCCAGGACCTCTGAGGGGGGCCCTGCTGCAGCCCAGGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_175551
Insert Size	6771 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_175551.4, NP_780760.2
RefSeq Size	8476 bp
RefSeq ORF	6771 bp
Locus ID	23856
UniProt ID	Q8C9B9
Gene Summary	This gene encodes a transcription factor involved in apoptosis. The encoded protein functions in cell cycle progression and plays a role in chromosomal stability. This protein regulates the self-renewal of embryonic stem cells. Disruption of this gene in mice causes symptoms similar to myelodysplastic/myeloproliferative diseases in humans. Mice lacking this gene show severely reduced fertility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (Dido3, also known as isoform 3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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