Rttn (NM_175542) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4921538A15Rik; AI666264; C530033I08Rik; D230040K24
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225233 representing NM_175542 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCTGGCGGGGCTCATCAGGAAGCTCGGCCATCAGCTGGCGGAGATCAGGGAACGCGCCCTCAAAA GTATCCTGTGCAAGATTGAGCACAACTTGATCTGCTATGCTGACTTGATTCAGGAAAGGTTGCTTTTTCT CCATTTGCTCGAATGGTTCAATTTCCCATCTGTCCCAATGAAAGAGGAGGTTCTGAACTTGTTAAGCAGA TTGGTTAAGTATTCTCCAGCCGTCCAACACTTGATTGACCTTGGTGCAGTAGAGTTTTTATCTAAGCTCC GTCCAAATGTGGAGCCAGTAATACAGGCTGAGATTGATGGCATTCTCGATGGACTTTTCATTCTTCCTTC AGAAGTTCCTGCACTGTGTTCTTCCAGTTACCACACTGATCAAATTGAATTGTCACAACAACCTGAAGTC TTAACAGGATATTTTCCCCAAGACAAAAGTAATTTCCAGCAGATGGAAGTACCACCAGGGCCAGCGGGAA ACCAGGCTGTGAAGTGGCTGAGGTTCTCTGTGTTTCCTTGGCTACCCCTGACCACAACAGACAGACATGT TCTCTCTTCCAATGAAAGCTCTTTGAGAAGTAGTAACCACACTCTGATCTGGAACACCTGTGAGCTTCTG AAGGACGTGATCATGCAAGACTTTCCTGCTGAGATTTTTCTTCAGAGGCCAAAGATTGTTCAGGGCCTTT TGTGTCTGGTGAAGCTGGCCTTCGGAGGAGATGGGAAGCATCGTCTAGCATTACAGTCAGTGTCCTGTTT GCAGCAGCTGTGCACAAGCTTAAGAAACAGACTCAACTTTTACAGAGACCCCAACTTTTTCTCCAGTAGA CAAGACACAGTCTCCCAGAATTCTTCTTTGTCTTACTGTCATGAAGCAAGAGGTCCTTACCATTCCCCAA ACCCATCCCCGGGAAGCAGTAGCTCTCGGCCTTCTGTGGTTGGGCGCACAGGCCAGCGACCCCGAGGAGA TGGTCAGGACTGGGATGCAGTGTCTTCCAGTGGAAGTAGTAGTCACACGCATGTGAACTCTAGGATATCT GTGCATTCTCCTTTGGACGTGGCACCCGTGGATCTGCCGGAGCTGGAAGCAGAGGACACACTGGAGCTGC AGTTCCAACAGCTTAGTCTTCCTCAGTTCTGCATCTCCACCCTGGAGGCAGCAGCTCCTCTGCTGAGAAC AGGCAGCAGAGAAATGATAATAAGAGTTCTGGAACTGCTTGCAGAGGACATGCTGCTCATTGGACAAGCA ATTTCATCAGAAATCTGGGATGATAACAGCCTTTTTGCTATCAATGTGAAAGAGAAACTGCTCCAAGTGT TGGGGTCCCTTGGAGAAACCATGTGCTACCGTAAAACCAGTTTTAGCATGGAGCAAGCCGAGGCTGTACT CGTGCACCACAGAATGGCCTTTGTCAGCATCTCACTGTTCACAGTTCGACTGTTGCAACTTCTTCTCCCT GTTGAAAAGGCGAGTGAGTTTTTGCCAGAGTCCATGTCAGCAGCATTATTTTTTGTCTCCTTGGACATGC CTATTTCTTTGGAATATCCAGAGATTCATGACACAGTTGTGGCTTATTTGGAACAGCTGGATTCTGAAAA TTACAGCATTTATAAGCGAACTGCAGAGGCTGTTTATTCAATTGAATGCACCTGTAACTTCCTATCTGAT GTGGGGAAAGAGGGAGAAAAGAATCTTCTAGAATTAGTGGAGCTGGCAGACCAAGCCTTGCGTAGCTTTT CCTATCATCAGCATTCTCCCCTAATAAAGGAAATCATCTGCATCTGTTCAAAGATCTGGAAATCTGCACA GGCTAGTCCATTACTCCAGGGAGAGAGTCAGAAGGTGTTTCTCCATATGCTGTCTCACCCATTGCCACAG GTGAAGGTGGAGACTTACCAGTGCTGCCTGGAGATTGTAAAGGAATGTTTAGGTGTCCAAAATGTCACTA AACCTGTGTCTTCACTTTGTAGTGGAATTCATTTTCTGCTTCATCCAAAAGTTCTGTATGAAATCTCTGC ATTTGGTATTCAAGAATCCAAAAATGAGGTGAATGCTGCTGCCAAGGCCATTCTGCTGTATCTGCTTCAG GGACGATTGATGATGACAGCTCTGACCTGGAACAAGTTTATCGAATCTCTCGCTCCTGTCATTCCAGTGC TACAGGGCTACGCTGATACAGAAGATCCCTTAGGCAACTGCATTCTCCTTCTAAGCAAAGTGAGCTCTGA GGCAGGAGAAGGCGCTCTCCCCTGCACTGCCCGGCTGAAGTCTGTGCTACGGCTTTTGCTAGTAAAAAAA CCTTCGGTTCGTTCACTAGCATTAAAACTTTTAGCATTTCATCTTACCAGTGAAGAAGGGGCTGATAAAA AGCGCCCGTTCATAGATGCTGGCGTTCTCTCCAGAGTTACTAATCTGTTTATTGTGAAAAAACCTATTGA CCTTAAGTTGGATGACAGAAGAGAACTAGTTATTAAGTTGGAGACTGTTAAAAAGGTGTGTGACATATTC ACCTCAGACGATGTTGATCTTGTTTTAAGAAAGTCAGCTGCAGAGCAACTAGCTGTGATAATGCAAGATA TTAAAATGCATACAGTGGTGAAAAAGTTATGCTTAGTTGAGAAGATTATTGGACATTTAAATGAATTTGT GGGTCAAGATGGTGAGGTTATAGAATGCTTGATTCAGCCATGCCTCACACTCCTGAGGAAGGTTTTGTAC GCTGACCCGGTTATCCGTGTTTCCTTGTCACAGCAGGCCTCTCTCTTGACCCTATTACTCAGAGTTTCCT TGATATTTCATGAAGATTGTACTGTGGTGGCTGAAGTCGGGGCATTGTTTTGTCTTCTATTGTTCGATGA AGTATCAAGAATGGATACATGGTCTGATACTCTTTCTGATAAGCCTTCTTTGTCATCAGTCTTCAGTTTG CCGGTTTCAGTCTTTAGAAGGTATCATCTACCAGTTCATGTGACTGGCCACCATGCTGTGAGTCCTTACT CAATAGTTTTGCCATTACCTACTGAATGTTTGACCCTGAAGCCGGTGTCAGATATGCTGAGAATAGCTTG GAACCTGGCATGGTATCATGGGAGTGATAATCTATTGAAACAAATGACCTCTGAAGCTAAAATTCAGGAA TTTTTAGATACACTGCAGCTGTCCACAGAAGATATCCTTGCACTGAAGATCACATACACGGATAGTGGGT TGAAGGACTGCCTTCAGTCCATCATTCAGGCTGGAGGCCACAGGGATGTGAGGGCTGCTATCACCAGGAT GAGTTTTTATCTTTTGAATGACAAACTGTCTTTGAAGGATGATATTGGTCCATGTGGCGTTACTTTAAAA TCCTTGGCTTGGCACACTGTGCTGAACAGGTTTTTACAAGTACTTCCTGCTTGCACTGAAGATGAGAAAC TGCTAATAGATATCATACATTTTTTGAATAAGTTAATAAAGGAACAAAGAAAAAACCCATCAATTGAACT TTTAAACTGGATTCTGGAATTACTTCTTAGGCATAATCCAAGTCCACTACTAGACCTGCTGGTTCTGACA GATTCACATGCAGGAGAAGAAACAGATGACGTCCGGACTGCTGTCAGACAGCAACTCCAGAAAGAACTGA TCAGTCTTCTCAATTCCTTGTTACTGGTTTTTATGACAGTCTCTGACAGGAAATGCTTGGAACTGTTTTA CGTTTTCCAAACACAGCTGTCTCTGAAGCTACTCCAGTGCCTCAGAGTCACAGATGCTCCCCATTTCTAT GGCTTACCTTCATTGGAGAGGACCTTAGCAGGGATGGCTCACCTCACTGCCTTCCCTGGGTGGAGTGCAC ACTCTCCTCTTTCAAAGCCACTTGAGATTTGTGGGAAGTACTTGTCCGGTCTCCTGGAGGTCATTACTTC CTTTTATGTTGAACGGGGAGGAAATGCTATGTCCTTCATGGGGAAAGGCGTTACAAAGAGCACAGGTCTT TGCTTACTTCACTTATCCCATGAGATGATAGTCCAGGCACCAGGCTCGGAGTGGATGCCGCTTTGGTTCT TGCCTTTGGGTAGTCACAGTGAAGAACATGCCCCTACTCAGCAAGGATTGGCCTGGCTAATTCCCTTATG GGTTGATAGAGACCCAGAGGTGAGATTCACTTCACTGGGGTTGGGATCAGCACTGACTACTATTGAAGCT GGCTGTGTGGCCTTGGCAAACAGTTGTCAGAATATTTCTGGTGGGCTCTGGGGAACCGTGGTGAACATTC TTCTGGATCAATCAGAATGCAGTCTGGTGCGCCGGGAGGCTGCATTTATTCTTCAGAATCTCCTTGTAAT TCCAATGCCTTCAGAAATTATAAAGGATTATACTTGGCAGGGCCCCTGTGTCCATGATGAGGATTCTGGC CTGTCACTTTCAGGAAAACCTGCCCTTCAGGCTCTTTTATATCACTGTCGTTTCTATGAACATTTGACTC ACATGGTGAAACATTGTTATGTAGGAAGATATATCTTTGATTTTAATTTTTCTGCTTTTACTGAAACTTC AGAATACAGTGATTTAAATGGTTTAGACGACTCATTCAAGTTTTGGAGGGCCCCTTCTGGGACATCTACT CAGGATCATGAGCCAAGCTCTCTTTCCACCTCAGAAACAATGGTGGCTGCCTCGGTGGTCAGTTCTGAGA TTCAGCCCCTGCTGCCGTCAACACTTCTTCCTGAAACTGCCCTTGACCAGTTTGTGACGCAAGGTCAACG TGAAATGAGACCAGTGCGACCTCGTGACTCATTGCTGTCTACTTCCCTGAACAGGCAGTATGTGTTTGTT ACTCCGTCTCTGCTGTCAGCAGTCTGTAGCCTTTTAGACAACCTCTTGGCAGTTACTCCAAGAGATGCTG CAAATGCTTTTCAACAAGCTCATCTCATAGAACTCCTCTGTAGCACGGTGGATGCTTCCCTCATAGAAAT GTGCATCCAAGAACTCAAGACCCCACTGCCTTTACCATCTGCTGTTGAACATATTCAGGCTCAGGCTTCC TTTTTCTTGGAATACCTATCCTCTTTGTCCCAGCTCTTGAAGTCCTGTCTGTTGGTAGAGCCTAAACTTG TGACACAAGATGAGCTCTTGAAACCTCTTATCACTAATGTCATCCGAGTTCTCATTGTGTGTCCCAAAGA TGTATTAGATGTACAGTTAGTGTTGGCTTTTTACCGCACATGGACGCATTTATTTGACCTTCTGGCCACA CTACTGAGGAAAGCTGGTGCTGTCTCCCTTGTTCCTCTTACCTCAGCTCTTGCCAAGCATTGGGAGGCAG TGACTGATACACTGTGTAGATGTGTGGGCCTCTCATTCAAGTGCCCTGCCCTGACTATTGCCAGCCTCCA GTTCCTGTCTGTTCTCTTGGCTGAGGAAGAAAAGAGGCGTGTCCAGGACAAGGACAAAACAAATGAGGGC CAGGCCCCAACAGTGGCCTTACTTCTTGATGGAACTCAGGGAAGCCTTAGCTCTTCAGAGCGACTTAATG AAACAATTCTTCAGTGCTATGAAGGAATATCCCCCAAAGATGTCCTGAAAAGAGTTGCTGCAAATGCCCT GTTGTCACTTCTGGCTGTTAGCAGAAGAGCGCAGAGGCATGCTTTGAGATCTGATCTCATAGAAAACTGT ATGGAGCAGATGAAGCACATCAATGCACAACTGAACTTAGATTTGCTGAGACCTGGGAAAGCAGTACTGA AAAAAAAGGAGGATGGCTTCATTAAAGAATTAAGCATCACCATGCAGCTCCTAAGAAACTGCCTTTATCA AAATGAAGAATGTAAAGTGGCAGCTCTTGAAGCACGTCTTGTGCCTGTATTGCACTCTCTCTGGCCTTGG CTTTTGATGGAAGATTCATTGATGCAAATTGCCTTGCAGTTGCTTTGTGTCTATACTGCAAATTTTCCAG CTGGCTGCAGTTCCCTTTGTTGGTCAAATTATGGACAGTGTATTCAAACTGCACACAGAGGCACCCCCAG TAGCTCCCTGATACTGTGTATCCTGAAACTGGCTTCTCAGATGCCTGCAGAGAACACCACCATTCAGCAG TTGGTTTTTATGTTTCTTTCAAACTTGGCCTTATCTCATGACTGCAAGGGAGTAATTCAAAAGAGCAACT TTCTACAGCACTTCTTGTCCCTGACATTGCCTAAAGGAGGAAACCAACATCTCAGCACTGTGGCCATTCT GTGGTTGAAGTTGCTCCTGAATATGTCATTTGGAGAAGATGGACAGCAAATGATCCTGAGGCTAGATGGC TGCTTAGACTTACTCATAGAGATGAGCAAGTACAAGCACAAGAGCAGCCCTCACATGCCTCTGCTGATCT TTCATAACATTTGCTTCAGTCCTGCTAATAAGCCTAAAATCCTGGCTAATGAAAAAGTTGTTACTCTTCT GGCTGCCTGTTTAGAAAGTGAGAATCAAACTGCCCAGAGGATTGGAGCAGCTTCACTTTGGGCTCTAACT TACAATTACCAAAAGGCAAAAGCAACTTTGAAAAATCCATCAATAAAAAGAAAAGTAGATGAAGCATATT CCATAGCAAAGAGAACTCTCTCAAACTCAGAAGAAAACCCTCTGAATTCCTACTATTTGAAATGCCTTGA AAATCTAGTACAACTGCTTAACTATTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_175542
Insert Size	6681 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_175542.3, NP_780751.2
RefSeq Size	7280 bp
RefSeq ORF	6681 bp
Locus ID	246102
UniProt ID	Q8R4Y8
Gene Summary	Involved in the genetic cascade that governs left-right specification. Required for correct asymmetric expression of NODAL, LEFTY and PITX2 (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.