Med13l (NM_172424) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2210413I17Rik; 6330591G05Rik; 9030618F05Rik; mKIAA1025; Thrap2; Trap240L
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225226 representing NM_172424 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGCCGCGGCGAACTGGGTAGCGAACGGGGCGAGCCTGGAGGATTGTCACTCCAACCTCTTCTCGC TGGCTGAACTCACAGGAATCAAATGGCGTAGGTACAATTTTGGAGGGCACGGGGACTGTGGACCCATAAT TTCAGCCCCAGCCCAAGATGATCCAATCCTGTTAAGTTTCATCCGCTGTCTGCAAGCCAACCTGCTGTGT GTATGGCGTCGTGATGTCAAGCCAGATTGCAAAGAGCTCTGGATATTCTGGTGGGGAGATGAACCCAACC TAGTGGGAGTAATACATCATGAACTGCAGGTTGTGGAAGAAGGACTCTGGGAAAATGGCCTTTCCTATGA ATGTAGGACGCTGCTCTTCAAAGCAATCCACAATCTGTTAGAAAGGTGCCTAATGGATAAGAACTTCGTG CGGATCGGGAAGTGGTTTGTGCGGCCCTATGACAAGGATGAAAAGCCAGTCAACAAAAGTGAGCATCTTT CCTGTGCTTTCACATTCTTCCTTCATGGAGAGAGTAACGTGTGCACAAGTGTGGAGATAGCTCAGCACCA GCCGATCTATTTGATCAACGAGGAACACTTACACATGGCCCAATCTTCACCTGCACCATTTCAAGTACTG GTGAGTCCTTACGGCTTAAATGGCACACTAACAGGGCATGCATACAAGATGTCTGACCCGGCCGCCCGCA AGCTGATTGAGGAATGGCACTGTTTCTACCCGATGGTGCTGAGGAAGAGGGAGGAGCCTCGAGAAGAGGC TGAGCTGGGGTATGACGACGACTTCCCGGTGGCAGTTGAAGTCATTGTTGGTGGCGTCCGGATGGTCTAC CCTTCGGCTTTCGTGTTGGTCTCTCAGAATGACATCCCAGTTCCTCAGAGTGGGCACGGCACAGTTGCAC AGCAGGGGCTTGGTAGTGTGAAAGACCCCAGTAACTGTGGGATGCCTCTGACGCCTCCCACCTCCCCAGA GCAGGTGGTTATAGGAGAGAGCGGAGGTGTGCAGAGTGCTGCCAGTCACCTCGGTTCCCAGGATGGAGGG ATGTCAACTATGCACAGTCCGAAGAGATCAAGGAAGACCCCTCCTAAACTGCACAGTCACATGGTCCGTC GAGTCTGGCGGGAGTGCATCCTCAGCCGAGCCCAGTCTAAGAGGAGCCAGATGTCGACGCCCACTCGGGA AGAAGAGGCTGCTCACAGTCCTGCTGCCTGGGATTTTGTGGACCCAACCCAAAGAGTCAGCTGCTCCTGC TCCAGGCATAAACTGTTAAAACGGTGTGCAGTAGGACCCAGCCGACCTCCCGCCATCTCTCAGCCAGGCT TCAGTGCAGGACTGCCATCATCTTCATCCTTACCACCCCCTGCTTCTTCCAAGCACAAAACCACAGAAAG ACAAGAAAAAGGAGACAAGTTGCAAAAGAGACCCTTGGTGCCCTTTCACCATAGGCCCTCTGTGGCCGAA GAATTATGCGTGGAGCAGGATGCGCCTGGGCAGAAGCTAGGGCTGGCAGGGATAGACGCCTCCTTAGAGG TGTCGAACACTCGAAAGTATGATAAGCAAATGGCCGTGCCTTCCAGAAATACAAGCAAGCAAATGAATCT GAATCCTATGGATTCTCCTCATTCCCCCATCTCCCCTCTGCCCCCCACACTCAGCCCTCAGCCACGAGGT CAGGAGGCAGAGAGTTTGGACCCACCTTCTGTCCCTGTGAACCCTGCCCTCTATGGAAATGGGCTAGACC TTCAGCAGCTGTCTACTATAGAGGACAGAACTGTCCTCGTAGGCCAAAGACTGCCTCTGATGGCAGAGGC CAGTGAGACAGCCTTGTACAGTGGGCTCAGGCCTTCATACACCGAGTCGTCAGACAGGTGGTGGCAGAGT TTTCGTCTCCCATCCAGTGAGGATGCTGAGTTCCGGCCTCCAGAGCTGCAGGGGGAGAGGTTTGACACTG CCCTGGACCTGAATCCCGAGAGCACGGCCCTGCAAAGACTCTTAGCACAACCTAACAAGCGGTTCAAAAT CTGGCAGGATGAGCAGCCTCAGGTGCAGCCTCTCCCCTTCCTCGACCCATCACCTCTGTCCCAGCAGCCT GGAGACACTCTGGGAGAAGTCAACGACCCATACACCTTTGAGGATGGCGACATAAAGTACATCTTCACAG CAAATAAGAAATGCAAGCAGGGGACGGAGAAGGACTCCCTGAAAAAGAACAAGTCAGAGGATGGATTTGG TACCAAGGATGTCACTACACCAGGTCATTCCACGCCGGTGCCTGATGGGAAAAATGCCATGTCTATTTTC AGTTCTGCTACTAAAACAGATGTCCGGCAGGACAGCGCTGCTGGCAGAGCCGGCTCTGGGAGCCTTACCC AGGTGACAGATTTGGCACCTTCCCTGCACGACTTAGACAACATCTTTGATAACTCTGATGAAGACGAGCT TGGGGCCGTGTCCCCTGCTCTACGTTCATCCAAGATGCCTACTGTTGGGACTGAGGAGCGGCCCCCTGGG AAGGACGGAAGAGCTGCTGGCCCTTACCCTCCAACAGTTGCAGACTTGCAAAGGATGTTCCCCACCCCTC CGTCATTGGAACAGCACCCTGCATTTTCTCCTGTGATGAACTATAAAGATGGAGTAAGTTCAGAGACAGT GACTGCACTGGGCATGATGGAGAGTCCCGTGGTCAGCATGGTCCCCACACACCTCACGGAGTTTAGGATG GAAGTGGAAGATGGCTTAGGAAGCCCCAAGCCAGAGGAGATAAAGGACTTTTCCTATGTGCACAAAGTGC CGCAGTTCCAGCCTTTCGTGGGATCCTCCATGTTTGCTCCACTGAAGACGCTGCCGAGCCATTGCCTGCT GCCTCTGAAGACACCTGACGCCTGTCTGTTCCGGCCCTCATGGGCGGTCCCTCCTAAGATGGAACAGCTG CCCATGCCCCCTGCAGCCTCTTCTATTAGAGACGGCTACAATAACGTGCCTAGTGTCGGGAGCCTTGCAG ACCCAGACTATCTGAACACACCACAGATGAACACACCTGTGACACTCAACAGCGCCGCCCCTGCCAGCAA CAGTGGAGCTGGAGTTCTCCCATCTCCAGCTACCCCTCGGTTCTCTGTCCCCACACCACGAACCCCCAGG ACTCCAAGAACTCCCAGAGGTGGGGGCACTGCTAGTGGCCAAGGGTCTGTGAAATATGACAGCACAGATC AGGGGTCACCAGCTTCCACCCCCTCTACCACAAGGCCCCTTAACTCTGTGGAGCCTGCCACCATGCAGCC AATTCCTGAGGCCCACAGTCTCTATGTCACCCTCATTCTGTCAGATTCTGTGATGAATGTCTTCAAGGAC AGGAACTTTGACAGCTGTTGCATTTGTGCCTGCAACATGAACATCAAAGGGGCAGATGTCGGGCTGTACA TCCCCGATTCCTCCAAGGAGGACCAGTACCGCTGCACCTGTGGGTTTAGTGCGATCGTGAACCGTAAACT TGGCTACAACTCGGGGCTCTTCCTCGAAGATGAGCTGGATATTTTTGGCAAGAATTCTGACATCGGCCAA GCTGCAGAAAGACGCCTGATGATGTGCCAGTCCTCCGGCCAGTCCACCTTGCTTCCTCAGGTGGAGGGTG CCCGGAAAGCCCCAGAGCCCCCCGTAAGCCTCCTCCTCCTCCTTCAGAATCAGCACACGCAGCCCTTCGC TTCTCTCAGCTTCCTGGATTACATCTCCTCCGCCAATCGCCATGCACTCCCCTGTGTGAGCTGGACTTAT GACCGTGTGCAGGCAGATAACAATGATTACTGGACAGAGTGCTTCAACGCATTGGAACAGGGGAGGCAAT ATGTGGATAATCCCACAGGCGGGAAAGTGGACGAGGCTCTGGTGAGAAGTGCCACCGTACATTGTTGGCC GCACAGCAACGTGCTGGACACAAGCATGCTCTCATCCCAAGATGTGGTGCGCATGCTGCTGTCCCTGCAG CCCTTCCTGCAGGATGCCATCCAGAAGAAGCGCACGGGCAGGACCTGGGAGAACATCCAGCATGTGCAGG GGCCGCTCACCTGGCAGCAGTTCCATAAGATGGCGGGGCGCGGGACCTACGGTTCCGAAGAGTCTCCTGA GCCGCTGCCCATCCCCACTCTGCTGGTGGGCTACGACAAGGAGTTCTTGACCATCTCGCCCTTCTCCTTG CCCTTCTGGGAGAGGCTGCTCTTGGAGCCCTATGGGGGCCACCGTGATGTTGCTTATATTGTGGTGTGTC CAGAAAATGAGGCCTTGCTCGAAGGAGCCAAAACTTTCTTCAGGGACTTGAGTGCTGTATATGAGATGTG TCGGCTGGGACAGCACAAGCCCATCTGCAAAGTGCTCCGTGATGGGATCATGCGTGTGGGCAAGACCGTG GCGCAGAAGCTGACAGAGGAGCTTGTGAGCGAGTGGTTTAACCAGCCATGGAGCAGTGAGGAGAGTGACA ATCATTCCAGACTCAAACTTTATGCTCAAGTTTGCCGCCATCACCTAGCACCTTATTTAGCCACTCTGCA GCTCGACAGTGGCCTGCTGATGCCACCTAAGCACCAGAGCCCACCAGCAGAAGCACAGGGACAAGCCACG CCTGGGAATGCTGGGTCTTTACCTTCGAATTCAGGCTCAGGAGCACCTCCAGCTGGCAGTGCATTCAATC CCACCTCCAGCAGCTCTGCAAACCCCACAACAAGTAGTTCATCTGCCTCCTCTGGGCCGCCAGGCTCCTC AGCTGCCTCTGCTCCAGGCATCACTCAGATGAACACTACCTCTTCTTCAGGATTCGGTGGTGGTGTTGGA GGGCAGAACCCCAGTGCGGGGGGCAGCTCTACAGATCGAACCCCAGGGAATGTGGCTTGTGGAGACACTG AGCCTGGGCAGAGCTGCACCCAGTCCTCACAGGATGGACAAGACAGTGTTACAGAGAGGGAGAGAATAGG AATCCCCACGGAGCCTGACTCTGCAGACAGCCATGCCTACCCTCCAGCCGTCGTCATTTACATGGTGGAC CCCTTCACCTACACTGCGGAGGAGGACTCCAGCTCTGGAAACTTCTGGCTGCTGAGCTTGATGCGCTGCT ACACGGAGATGCTGGATCACTTACCCGAGCACATGAGAAGCTCGTTCATTCTCCAGATTGTGCCTTGCCA GTACATGCTGCAGACCATGAAGGATGAGCATGTCTTCTACATTCAATACTTGAAGTCCATGGCGTTCTCA GTGTACTGCCAGTGCAGGCGGCCTCTGCCTACACAGATCCACATTAAATCCCTCACAGGCTTCGGGCCTG CAGCCAGCATTGAGATGACCCTCAAGAACCCGGAGCGGCCCAGTCCAATCCAGCTTTATTCCCCTCCCTT TATATTGGCTCCAATCAAAGACAAGCAGACAGAACCGGGAGAGACCTTTGGTGAGGCGAGCCAGAAATAC AATGTGCTCTTTGTGGGCTATTGTCTGTCTCATGACCAGCGCTGGCTCTTGGCATCCTGCACTGACCTGC ATGGGGAGTTACTGGAGACCTGTGTTGTAAACATTGCTTTACCGAGCAGGTCACGGAAGAGTAAAGTGTC TGCACGGAAGGTCGGGCTGCAGAAGCTGTGGGAGTGGTGCCTGGGCATTGTCCAGATGACTTCTCTGCCC TGGAGAGTTGTCATTGGCCGCTTGGGGCGTCTTGGCCACGGGGAGCTGAAAGATTGGAGTATCCTCCTTG GAGAATGTTCACTGCAGACAATCAGCAAACAGCTCAAGGATGTGTGCCGAATGTGTGGGATCTCTGCTGC AGACTCTCCTTCCATCCTCAGTGCCTGCTTGGTTGCCATGGAGCCCCAGGGGTCCTTTGTAGTGATGCCA GATGCTGTCACAATGGGCTCTGTGTTTGGCCGAAGCACTGCATTGAATATGCAGTCGTCCCAGCTGAACA CACCTCAGGACGCCTCCTGCACCCACATCTTGGTGTTCCCGACATCATCAACCATCCAGGTGGCTCCAGC CAACTACCCCAATGAGGATGGGTTTAGCCCCAACAATGATGACATGTTTGTAGACCTTCCATTCCCAGAC GACATGGACAATGACATTGGCATCCTCATGACCGGGAACCTCCACTCCTCTCCCAACTCCTCTCCGGTTC CCTCCCCGGGCTCTCCCTCTGGAATCGGTGTGGGCTCTCACTTCCAGCACAGTCGGAGCCAGGGAGAGCG GCTTCTGTCTCGGGAGGCTCCAGAGGAACTCAAGCAGCAGCCCCTGGCCCTTGGGTATTTTGTGTCCACT GCCAAAGCGGAGAACCTCCCGCAGTGGTTTTGGTCATCGTGCCCCCAGGCCCGGAACCAGTGCCCACTCT TCCTGAAGGCTTCCCTGCATCACCACATCTCTGTAGCGCAGACCGATGAGCTCCTTCCTGCCAGGACCTC TCAGCGGGCTCCACACCCTCTGGACTCCAAGACCACATCTGACGTTCTAAGGTTTGTGCTGGAGCAGTAC AACGCCCTATCCTGGCTCACGTGCAATCCAGCCACCCAGGACCGCACTTCCTGCCTTCCCGTCCACTTCG TGGTGCTCACGCAGCTGTACAATGCCATCATGAACATGCTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_172424
Insert Size	6624 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_172424.4, NP_766012.3
RefSeq Size	9315 bp
RefSeq ORF	6624 bp
Locus ID	76199
UniProt ID	Q6JPI3
Gene Summary	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.