Nup214 (NM_172268) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BC039282; CAN; D2H9S46E
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225163 representing NM_172268 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACGAGATGGATGCCATGATCCCTGAGCGGGAGATGAAGGACTTTCAGTTTAGAGCACTGAAGA AAGTGAGGATCTTTGACTCTCCTGAAGAGTTGCCCAAGGAGCGCTCGAGTGTGCTTACCATATCCAATAA GTATGGTATGCTCTTCGCTGGTGGCACCAATGGCTTGAATGTTTTTCCTACTAAAAGCCTTCTTATCCAA AATAAACCTGGAGATGATCCCAATAAAATTGTTGACACAATTCAAGGCTTGAATGTTCCTATGAAATTCC CAGTCCATCACCTAGCACTGAGCTGTGATAGTCTTACACTCTCTGCCTGCATGATGTCTAGTGAATACGG CTCCATTATAGCTTTTTTTGATGTTCGCACATTTTCAAATCAGGCTAAACCATTGAAACGTCCATTTACC TATCACAAGGTTTCAAACGATGCCAGCGGAATGGTGAATGATATGAAGTGGAACCCCACTGTCCCTTCCA TGGTGGCAGTTTGTCTGGCTGATGGCAGTATTTCTGTCTTGCAAGTTACAGATGTGGTGAAAGTATGCGC CACACTTCCTCCTTCCACAGGAGTCACATGTGTATGCTGGAGCCCCAAAGGAAAGCAGCTGGCAGTAGGA AAGCAGAATGGAACTGTGGTCCAGTACCTTCCTACTTTGCAGGAAAAAAAGGTCATTCCATGTCCTCCAT TTTATGAGTCAGATCATCCTGTCAGAGTTCTGGATGTGCTATGGATTGGTACATATGTCTTCACGATAGT CTATGCTGGTGCAGATGGAACCTTGGAGACCTGTCCAGATGTGGTGATGGCTCTACTCCCGAAAAAAGAA GAAAAGCATCCAGAAATATTTGTGAACTTCATGGAGCCCTGCTACAGCAGCTGCACAGAGAGACAACATC ATTACTACCTCAGCTACATCGAGGAGTGGGATTTAGTGCTGGCAGCATCTGCAGCTTCTACAGAAGTTAG CATCCTTGCTCGACAAAATGATCAGACTAATTGGGAATCTTGGCTGCTGGAGGACTCTAGTCGAGCTGAA CTGCCTGTGACAGACAAGAGTGATGACTCCTTGCCCATGGGTGTTGCCATAGATTATACCAATGAAGTGG AAGTCACCATCAATGAAGAAAAGACTCTTCCGCCTGCTCCGGTTCTCCTGTTACTTTCAACAGATGGTGT GCTTTGTCCATTTTATATGATCAACCAAAATCCTGGGGTTAGGTCCCTAATCAAGACCTTGGAACTGATC TCAACAGAAGGAGAGCGACAGCCTAAGTCCTCAGGAAGTTTTCCTGGTACTCCATCTTCCCCTCAAGCTC CACAAAACCTGGACGCTCCAGCAACAGCCTCTTCGCCTCTTCCACCTGTGTCCGCTGCTCCCACCTCCAC CTTCCCTATGCCCTCTGCTGCAGGAAGCCCTTCTGTGTTCTCCTTTGGCCCTTCATCCTTCAAGTCTTCT GCTTCTGTCACCGGGGAGCCCCCACTGTACCCCACTGGCTCCGACAGCTCCAGAGCAGCACCAGGCTCTG GCACCTCCACTTTCTCTTTTGCTCCTCCTTCCAAAGGCTCCCTGGCGTCGACCCCTGCAGTGGCTCCTGT GGCCACATCTGCTGCTCCCTTCACTTTTGGTTTTAAGCCCACCTTGGAAAGCACACCTATGTCCAGTACC CCAAACACAGGGATGAAGCCTTCGTTCCCTCCCTCGGCCTCTTCGGTGAAAGTCAACCTGAATGAGAAGT TCACCGCTGTGGCCTCCTCTGCACCTGTTCATAGCTCCACGAGCACACCCTCCGTGTTGCCGTTCTCCTC CTCCCCGAAGCCAACGGCTTCTGGACCACTCAGCCATCCTACGCCTCTCCCAGCATCATCCAGCTCCATG CCTTTGAAGTCTTCGGTCTCTCCCTCGCCAGCAGCTGGCCGCTCCACTCAGACTGCGCCAAGTTCAGCAC CCTCCACAGGGCAGAAATCACCCAGGGTAAACCCTCCAGTGCCAAAGTCAGGTTCTTCTCAGGCCAAGGC ACTTCAGCCTCCTGTCACAGAGAAACAGAGGCCTCAGTGGAAAGACTCAGACCCCGTGCTGGCTGGGATT GGAGAGGAGATTGCACACTTCCAGAAAGAACTAGAGGAGTTAAAGGCCCGGACCGCCAAAGCCTGTCTCC AGGTTGGCACCTCAGAAGAGATGAAGATGCTGCGGACAGAGTCAGATGACTTGCACACCTTTCTTTTTGA GATTAGAGAGACCACAGAGTCTCTTCATGGAGACATAAGTACTCTGAAAACAACTTTGCTGGAGGGCTTT GCTGGGGTCGAAGAAGCCAGAGAACAGCACGGAAGAAATCACGACTCTGGGTATCTCCACCTGCTTTATA AAAGACCGCTGGATCCCAAGAGCGAGGCCCAGCTCCAGGAAATTCGCCGCCTTCACCAGTACGTGAAATT TGCTGTCCAGGATGTGAATGACGTCCTAGACTTAGAGTGGGATCGGCATCTGGAGCAGAAGAAGAGACAG AGGCGCCTAATTGTGCCAGAACGAGAGACACTTTTCAACACGCTAGCCAACAACCGGGAGATCATCAACC AGCAGAGGAAGAGGCTGAATCAGCTGGTGGACAGTCTGCAGCAGCTCCGTCTCTATAACCACACGGCCCC GTGGAGCCTGCCCTCAGCGCTCTCAACTCAGAGCAACAGTCACAGTTTTGACAGTGACCTTGAATGCCTG CTAAAAACTACCATTGAATCTCACACCAAGCCTTCTCCCAGAGTGCCAGGTAAATTGTCCCCGGCGAAAC AGGCCCAACTGAGGAACTTCTTGGCCAAAAGGAAGACCCCACCAGTGAGGTCTACTGCTCCAGCCAGCCT GTCCCGGTCAGCCTTCCTATCTCAGAGGTACTATGAAGACTTAGATGAAGGCAGCTCCGCGTCATCCGTA GCTCAGCCTCTGGAGGGAGAAGATGCCCGGCCAACCTGCACATCTGTAGCTCAGCCTCTGGAGGGAGAAG ATGCCCAGCCAATCTGCAAAGAGGAAGAGGCTGTGGTCCCAGTACCTCGGCATGCCCCTGTGGTCCGCAC ACCTTCTATCCAACCCAGTCTCCTGCCTCAGTCGATGCCCTTTGCAAAGCCTCACCTGATCCATAGTTCT TCACCTGCCGTGATGAGCTCTGCAGTCTCTACGTCAGCCACCAAAGTTATTCCTCAGGGAGCAGACAGCA CAATGCTGGCAACTAAAACAGTAAAGCACGGTGCACCAGGTCCTTCCCACACTGTTGCAGCTCCCCAGGC CGCAGCTGCTGCAGCCCTAAGACGGCAGATGGCCAGTCAGGCACCAGCTATGAGCACTCTGACCGAATCA ACTCTGAAGACTGTCCCTCAGGTGGTGAATGTACAGGAACTGAGGAGTAATCCCTCGCCCCCATCTGCGG CCATGGGTTCTGCCGTGCAGCACTCAGCAGCCAAAACACCCCATGCTGTGTTAACCCCCGTGGCTAACAG CCAAGCCAAGCAGGGGTCTCTGATAAATTCTTTCAAGCCGTCAGGACCCACAGCAGCATCCTGTCAGCTG TCCTCTGGGGATAAAGCTGTAGGTCAAGGGACAGCCAAGACAGAGTCCGCTGCGACCTCGACTCCATCTG CTGCAGGACAGCTCAACAAGCCTTTCTCATTCGCCTCCCCAGGGACCTTTACTTTTGGGACAATCACACC AACCCCATCTTCTAGTTTCACTGCCACACCAGGGGCAGGACCCCCTACTAAAGAGCCAACCCAGCTTGAG GCATTCTCATTTGGCGGGGGAGGCAAACCTTTCTCTGAGGCCATTCCTGGGAACTCCCCTGCCACAGGAG CAACATCGGCACCCAGCACTTCTGTAACAGCTGCCTCTCTGGAAGACTCTGCTCCATCCAGCAGCAAACC TGCAGCTCCTCCTGAAACTACTGTGTCCTCTGCTTCTAGCAAATTGGAAACTCCACCGTCCAAGTTAGGA GAGCTTCTGTTCCCAAGCTCTCTAGCTGGAGAGACGCTGGGGAGCTTCTCGGGCCTGCGTGTTGGCCAAG CTGAGGATTCTACTAAGCCAGTTAGTAAGGCCTCCTCCACAAACCTAGCTGGAGCCCAGCCAGCTAAGCC CTCAGGGGTTAGCTTTCCTAATACTTCAGTGTTAGGGAAGCCAGTGGAGCCCGCTGTGACCTCCTCTGTG AGCCCCGCCCCAGCAGCACCAGCCTCAGCTCTGAATGTGAGCACTAGCAGTTCCTCAGCAACTGTCTTTG GCAGTGTGCCCCTTACCAGTGCTGGACCTCCTGGGCTCATCAGTTTTGGTGGGGCTGCTTTGGCTAGTAG CAAAGCTAGTTTCTCATTTGGAAACCAGCAGACTAGTAGTAGTACAGCATCATCAGCTACCCCAACATCG ACCTCAGTTGCCCCTTCCCTTCCAGCATCTTTCCCTACTTTGTCATTTGGTGGCCTCCTGAGTTCACCAT CAGCTTCTTCCCTGCCCGTGTCCTCTGGTAAGAGTACAGAGGAGGCAGCACCACCAGCTGTTCCTGACAA GTCAGACAGCAGTGAAGTTTCAGCAACAACACCCTCACTTCCAGTACAGCCACAGTCAACCCAGGCATCT CTGCAAACTTCTGACCCTGTCAAAAAAGAACCTGTTCTTGTGCAGACCACAGACAGCAGCCCCAGCAGAC CAGCATCTTCTGCCAGCTTTGTGGCATCCACAGAGTCCATGCCAGTAACCCTAGGGGCCCCTGACAGCAA GATAGAGGCAGTGTCTCCTGCGTCCACCTTTGCAGGGCCTGGACAGGCTGCTGTGGCAACAGCTGTTCTC CCAGGAGCAGGCTCGGCAGCCACTGAAGCATCGGGTACCCCTACCACCTCCACTGTCTCCAGCAGTAGCC CAACTTCTGCCACAGAGACAGCTGTATTTGGGACTGCCACATCTGGTTCCTCAGTCTTTACACAGCCACC TGCTGCCAGTTCTAGCTCAGCGTTCAGCCAGCTCTCCAGTAACACAGCCACTGCGCCCTCTGCCACCCCT GTGTTTGGGCAAGTGGCAGCCAGCATAACCAGCACAGCCGCTGCCACACCACAGGCCAGCAGTTCAGGGT TTGGCAGCCCTGCATTTGGTGCTTCAGCTCCTGGAGTGTTCGGACAGACAGCATTTGGGCAGACCCCAGC CTTTGGGCAAGCAACAAGCAGCCCTGCCAGTGGCTTCTCCTTCAGCCAGCCTGGGTTCAGTTCCGTGCCT GCTTTCGGCCAGTCTGTCTCCTCTACCCCCGCATCCACCAGTGCAAATGTCTTTGGAGCCACCTCGAGTA CCAGCAGTCCTGGTTCCTTCTCATTTGGACAGGCTTCTACCAATACAGGAGGGACGCTGTTTGGTCAAAA CAACCCTCCTGCTTTTGGTCAGAGCCCTGGCTTTGGGCAGGGAAGCTCTGTGTTTGGTGGCACCTCGGCC ACTACCTCTACAGCAGCACCTTCAGGGTTTAGCTTTTGTCAAGCCTCCGGTTTTGGGTCTAGTAATACTG GTTCTGTGTTTGGCCAGGCAGCCAACACTGGTGGGTCAGTCTTTGGCCAGTCATCCACTTCCAGTGGTGG CGTGTTTGGGTCTGGAAATGCTACAAGAGGGGGCGGTTTCTTCAGTGGCCTTGGAGGAAAACCCAGCCAG GATGCTGCCAACAAAAACCCCTTCAGCTCAGCCGGTGGGGGCTTTGGATCTACAGCTGCCCCAAATACCT CTAATCTGTTTGGAAACAGTGGAGCTAAGACATTTGGAGGGTTTGGCAGCTCATCCTTCGGGGAACAGAA GCCTGCCGGCACATTCAGCTCTGGCGGTGGGAGCGTAGCATCCCAAGGTTTTGGATTTTCCACTCCAAAT AAAACAGGTGGCTTCGGTGCTGCTCCAGTGTTTGGCAGCCCTCCTACTTTTGGGGGATCCCCTGGGTTTG GAGGGGTGCCAGCATTCGGTTCAGCCCCAGCCTTTACAAGCCCTCTGGGCTCGACGGGAGGCAAAGTGTT CGGAGAGGGTACTGCGGCTGCCAGCGCAGGCGGATTCGGGTTCGGGAGCAGCGGCAATACTGCATCCTTC GGTACCCTCGCCAGTCAGAACGCACCCACCTTCGGGTCCTTGTCCCAGCAGACCTCAGGCTTTGGGACCC CGAGCAGTGGATTCGCTGGGTTTGGATCGGGCACAGGAGCCTTCACCTTTGGATCATCTAACTCGTCTGT CCAGGGCTTCGGTGGCTGGAGAAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_172268
Insert Size	6258 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_172268.2, NP_758472.2
RefSeq Size	8195 bp
RefSeq ORF	6258 bp
Locus ID	227720
UniProt ID	Q80U93
Gene Summary	May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.[UniProtKB/Swiss-Prot Function]

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