Heatr5b (NM_001081179) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2010013B10Rik; A230048G03Rik; AV071430; D330050P16Rik; mKIAA1414
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225160 representing NM_001081179 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTAGCTCACAGTTTATTGCTAAATGAGGAAGCTTTGGCTCAGATCACGGAGGCAAAGAGACCCG TGTTCATCTTTGAATGGTTGAGATTTCTTGATAAAGTCCTAGTTGCTGCCAACAAGACAGATGTAAAAGA AAAACAGAAAAAACTTGTTGAACAATTAACTGGATTAATAAGCAGCTCACCTGGACCACCTACTCGAAAA TTGTTAGCTAAAAACCTTGCAGCCCTTTATAGCATTGGGGATACTTACACAGTTTTTCAAACACTAGATA AATGTAATGACATTATCAGAAGTAAGGACGACACTGCAGCCTACTTACCAACAAAATTGGCAGCAGTGGC TTGTGTTGGAGCCTTCTATGAGAAGATGGGAAGGATGTTGGGCAGTGCATTTCCAGAAACAGTGAACAAT CTGTTGAAATCTCTGAAAAGTGCAGAGTCTCAAGGGCGAAGTGAGATCTTAATGAGTCTGCAGAAAGTGC TGACTGGACTGGGCGGCGCAGCAGCTTCCTCTCACCGCGACATTTACAAGAATGCTCGATCCCTCCTGAC CGACAGGTCGATGGCTGTCCGATGTGCAGTGGCCAAGTGCCTTCTGGAACTACAGAATGAAGCTGTGTTC ATGTGGACTGCCGAACTAGAAAATGTAGCTACCCTCTGCTTTAAGGCTCTGGAAAACTCAAATTATGGGG TACGAGTCGCAGTATCTAAGCTACTAGGAACAGTCATGGCTACAGCATTGATGCCGAAACAGGCAACAGT CATGCGGCAAAATGTGAAGCGAGCAACCTTCGATGAAGTCTTAGAACTCATGGCCACGGGATTTCTGCGC GGAGGGTCGGGTTTCCTAAAGAGTGGCGGAGAAATGTTGAAAGTTGGAGGGTCTGTTAATCGGGAAGTGA GAGTTGGTGTGACACAGGCCTATGTTGTCTTTGTGACAACTCTGGGTGGCCAGTGGCTGGAGCGTAGCTT TGCCACATTCTTGTCCCACGTACTTGATCTGGTTTCCCATCCTCGAGCAACACAAACACACGTGGACGCT GTGTACTCCAGGCGATGTGTGTCCTTCATGCTGAGAGCTACCGTGGGCAGTCTGTTAGGTGAGAAAGCCC AGATAGCAGCTGCCAAGGAGATCTGCCAGGCAATCGGAAAGCAGATGAAAGCTGTGGAAGCAGTAGTGAA TGATACGAGCAGTGAAAACAAATCGGGGACAGCAGACATTGCAGCCAGCCAGCACGTCATGGTCTGTGCC CTGCAGGAGCTTGGGAGCCTGGTGCAGAGCCTGAATGCCACCGCCTCTCCTCTTATTCAGGAAGCGTCCA TAGGACTTTTGGAGATTGTGACGTCAGTGCTTCTTCACCCAAGCATGGCTGCTCGGCTTGCTGCTGCCTG GTGTCTGCGTTGTGTAGCTGTGGCGCTGCCCTTCCAGTTGACACCATTTCTGGATAGGTGTGCAGAACGG CTCAACAACCTCAAGACATCCCCAGAAGCTGTTAGTGGATACAGTTTTGCAATGGCCGCCTTGTTGGGTG GAGTGCATCAGTGTCCTTTGGGCATTCCTCATGCCAAAGGAAAGATGGTGGTCAGCATTGCTGAAGATCT GCTACGAACTGCTGCCCAAAACAGCAGGCTGTCCCTGCAGCGCACCCAGGCTGGCTGGCTTCTTCTGGGA GCCCTCATGACTTTAGGACCGTCTGTTGTTCGTTACCATCTACCCAAGATGTTGCTGCTGTGGCGGAACG TGTTCCCTCGCTCTTTAAAGGAACTGGAGGCTGAGAAGGCCCGGGGGGATTCATTCACCTGGCAGGTCAC TCTGGAAGGTCGCGCTGGGGCTCTGTGTGCCATGAGGAGTTTTGTTGCACATTGTCCTGAGCTGCTAACA GAGGATGCAATTAGGAAATTAATGACTCCTATCGAATGTGCCATGACCATGATGTCGCACATTCCATCCG TCATCAAGGCCCATGGAGCCCATCTGAAAGCTAGTGCCGCAATGGTCCGCTTAAGACTTTATGATATCTT GGCTTTATTACCTCCAAAAACTTATGAAGGGTCTTTCAATGCACTTCTTAGAGAGCTGGTGGCAGAGTTC ACTCTGACTGACAACTCGGCCAACACAACTACCTCCCTCCTTCGCTCCCTCTGCCACTATGACGACAGCG TTCTCCTGGGCTCCTGGCTTCAAGAGACTGATCATAAATCCATTGAAGACCAGCTCCAGCCAAACAGTGC ATCTGGCAGTGGGGCTCTGGAACACGACCCTTCTTCCATTTACCTACGGATACCTGCAGGTGAAGCCGTG CCGGGTCCGCTCCCTCTCGGAGTCTCAGTGATTGACGCATCTGTGGCTCTGTTCGGGGTTGTGTTTCCAC ATGTTTCCTATAAACACAGGTTACAAATGCTGGATCACTTTGCTGAATGTGTTAAGCAAGCCAAAGGTGT CCGCCAGCAGGCTGTGCAGCTTAATATATTTACTGCTGTTCTTAGTGCACTGAAGGGCTTAGCTGAAAAC AAGAGTACTTTGGGACCTGAGGAAGTCCGCAAGTCTGCTCTGACACTGGTTATGGGAGCCCTCGACAACC CAAACCCCATCTTACGGTGTGCGGCAGGGGAAGCCCTTGGAAGAATGGCTCAGGTGGTGGGAGAAGCCTC TTTCATTGCCCGAATGGCCCAATACAGCTTTGACAAGTTGAAGTCAGCTCGAGATGTCGTGTCCAGGACT GGTCACTCACTGGCCCTCGGTTGTTTACATCGTTATGTTGGTGGCATAGGATCAGGACAACACCTAAAGA CCAGTGTCAGCATTTTACTGGCTCTAGCTCAAGATGGGACATCCCCTGAAGTTCAGACGTGGTCTCTTCA TTCACTCGCCCTGATAGTGGATTCCAGCGGCCCAATGTACCGTGGGTACGTGGAGCCCACGTTGTCCCTG GTACTCACCTTGCTGCTGACAGTTCCACCTTCACACACAGAAGTTCACCAGTGCCTGGGCAGATGCTTGG GGGCGATCATAACTACTGTTGGACCAGAGCTGCAGGGAAATGCAGCGACCATATCCACCATCCGCTCCTC CTGCTTGGTGGGCTGTGCCATTACCCAGGACCACTCTGACTCCCTTGTTCAGGCAGCTGCCATCTCTTGC CTTCAGCAGCTTCACATGTTTGCCCCACGACACGTCAATCTGTCCAGCCTTGTTCCTAGCCTCTGTGTTC ACCTGTGCAGCTCCCACCTGCTGCTCCGACGAGCGGCTGTGGCCTGTCTCCGGCAACTCGCACAGAGAGA AGCAGCCGAAGTGTGTGAATACGCCATGAGCCTGGCGAAGAATGCAGGGGACAAGGAGATCAGCGGTGGT AATGTCAACCCTTTCACGCCTGGAGTTAGCTCTCGCAGTGACGTCCATTGCCGGCACCAGGGTGTTAACA TAACAGACACGGGGCTTGAGGGACTTCTCTTTGGAATGCTAGATCGAGAGACAGACCGGAAACTCTGCTC TGATATTCACGACACGTTGGGGCACATGCTCTCTTCACTGGCCGTTGAAAAGCTTTCCCACTGGCTAATG CTTTGTAAAGATGTCTTAGCAGCTTCTAGTGATATGAGTGCAGCCACACTCTTAAGCAGTGGAAAAGATG AAGAATCTGAGAAGAAAGATGAGATGGATGACGACGCCATGTTCACGACACTAGGCGAGGAGGACAAATC AAAGCCTTTCGTGGCTCCTCGCTGGGCCACTCGGGTGTTTGCTGCAGACTGCCTGTGCCGAATCATCAAC CTGTGTGAGAACTCAGACCAGGCTCACTTCGACCTTGCCTTGGCACGGTCTGCTAAACTACGAAACCCAA AAAATGACCTCCTAGTATTGCATCTCTCTGACCTGATTCGAATGGCATTCATGGCTGCAACTGATCATAG CAACCAACTGCGAATGGCTGGGCTCCAGGCACTTGAAGACATTATCAAGAAATTTGCATCTGTGCCTGAG CCAGAATTTCCAGGTCATGTGATCCTGGAGCAGTATCAAGCTAATGTGGGAGCTGCCCTAAGACCAGCCT TTTCACAAGATACGCCATCAGACATAATAGCGAAAGCTTGCCAGGTGTGCAGCACGTGGATTGGAAGCGG CGTTGTCAGCGACCTCAATGATCTCCGCCGGGTGCATAATCTACTGGTTTCCTCTCTGGACACAGTTCAG GCTGGAAAGGGATCTTCTAGTCAGCTGTACCGAGAGAGCGCCACCACCATGGAGAAGCTGGCCGTTCTTA AGGCATGGGCAGAGGTATATGTGGTTGCTATGAATATTAAGAAAGAAGCAGAGTCAAAACCAAAGAGAGC AATGAACAATCCTGATGATGATGATGACGACTATGGCACCATCGACGAGCTGCCTCCAGACAGCTTAATA ACCCTGGTGCAGCCTGAGCTGCCGACGCTCAGCCGCCTGTGGTTGGCAGCACTGAAAGACTACGCGCTTT TGACTTTACCAGCTGAATTTTCTAGTCAGCTTCCCCCAGATGGGGGGGCATTTTATACTCCAGAGACCAT CGATACAGCAAGACTTCACTACAGGAACTCCTGGGCCCCAATTCTCCATGCAGTGGCACTCTGGTTAAAC AGCACAGGATTTATAAGTCAAGAGTCCACAGAAGCAACAACTGTGTCTGGTGTCCAGAAACGCTCCCCAG CTGTCAGTTTAAACCAAGTTCCAGGAGCAATGGCTAGTGCTAAACCCTTGCCAGAAGTTAACAAAGACAG GATGCACCTGATCCTAGGTGTGAGTATACAGTTTCTTTGTTCCCCTCGACCAGAGGAGCCCATTGAACAT GTTACAGCATGCCTGCAGGCCTTGCACACCTTACTGGGATCTCCTTATGCACGGATCCACATCGCAGAAG ATCAGCTGATTGGCGTTGAGTTGCTAAGTGTCCTGCACCGCCTGCTACTGACATGGAACCCACCCTCTAT CCAGCTGCTGGTCACTGGAGTTGTGCAGCAGATCGTCAGGGCTGCTCAGGACTATCTACAGGAAAAGAGA AACGCTCTAAATGAAGAGGATATGGAAAAAGAGTCCTGTCCTACATTAGGAGAAGGGGGTGATACCGGTG GCCTCATTCCTGGAAAGTCCCTTGTGTTTGCAACCATGGAGCTGCTGATGTTCATTTTAGTACGGCACAT GCCACACCTGAGTACCAAGATGTTAGACTCTCCTAGTCACACGGCCATGAAAACTCAGCTGTCAGAAGAA AGCGCCCGCTTGGTGGCAGCCACGGTGGCCATTCTCTCGGATCTGCCGTCCCTTTGTTCACCGGCTGGAT GTATGACAATCCTACCCACAATCCTTTTCTTAATTGCAAGAATATTGAAAGACACAGCAATAAAGTCTGC GGACAACCAGGTTCCTCCCCCAGTCAGTGCAGCTCTTCAGGGGATAAAAAGTATTGTGACACTTTCCATG GCCAAAACTGAGGACACACAGAAACAGTGGACGACTCTAATCCGGAGCACCCTTGCCTGCATCCTGGAAT ATTCTCAACCAGATGACTGTATGCCCGCACCTGACGAAGTGAGCACACTCACAGCCATTGCGCTCTTCCT GTGGTCTGCCAGCAGTGAAATAATAGGAGTGCAGTCCCTACAGAACGGCTGCATGAACAGATTTAAGAGT GCCCTGAACTCGTGTGACCCATGGGTTCAAGCCAAATGCTACCAGCTTCTCCTCTCCGTCTTCCAGCATT CCAACCGTGCACTCTCCACTCCCTACATCCACTCCTTAGCCCCGCTGGTGGTTGGGAAGCTGAAAGCTGT GGAGAGACACAGACCGGCCAGTAGCACGGAACTTTTGGCTGTCCAAGAAGGAATTAAAGTTCTTGAAACA TTGGTTGCTCTTGGGGAAGAACAGAACAGAGTCCAGTTACTCGCTCTTCTAGTGCCCACTTTGATCTCTT ACCTGCTGGATGAAAATTCTTTTGCCTCAGCAAGTTCGATCTCCAAAGATCTTCATGAGTTTGCACTGCA GAACTTAATGCATATTGGACCTCTGTACCCACATGCTTTCAAGACGGTAATGGGGGCTGCTCCTGAACTG AAAGCACGACTAGAAACTGCTGTCCGAGCAAGCCAGGCGAGCAAAGCTAAAGCCGCTGCCAGGCAGCCCG CCCCCACCACACATTCCACACCGACAATTAAGCTGAAAACAAGCTTTTTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081179
Insert Size	6213 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081179.1, NP_001074648.1
RefSeq Size	6437 bp
RefSeq ORF	6213 bp
Locus ID	320473
UniProt ID	Q8C547
Gene Summary	Component of clathrin-coated vesicles (By similarity). Component of the aftiphilin/p200/gamma-synergin complex, which plays roles in AP1G1/AP-1-mediated protein trafficking including the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.