Dock11 (NM_001009947) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5033414A21Rik; 8030476J24; sph238; Zizimin2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225158 representing NM_001009947 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAAGTGCGCAAATTCACCAAGCGGCTGAGCAAGCCAGGCACGGCAGCCGAGCTCCGCCAGAGCG TGTCGGAGGCCGTGCGGGGCTCCGTGGTGCTGGAAAAGGCCAAGTTAGTTGAACCCCTGGACTATGAAAA TGTTATCACTCAACGGAAAACCCAGATCTACAGTGATCCCCTCAGAGATCTGCTTATGTTCCCCATGGAA GACATATCTATCTCAGTGATTGGTCGTCAGCGCAGAACAGTGCAGTCTACTGTACCAGAGGATGCTGAAA AGAGGGCCCAGAGTTTATTTGTCAAAGAGTGTATTAAGACCTACAGCACAGATTGGCATGTGGTGAACTA TAAGTATGAGGATTTCTCTGGGGACTTTCGGATGTTGCCATGCAAGTCTTTGAGACCTGAAAAGATTCCT AATCATGTGTTTGAGATTGATGAAGATTGCGAGAAAGATGAGGATTCATCTTCTTTATGTTCTCAGAAGG GTGGTGTGATAAAACAAGGTTGGTTGCATAAAGCAAATGTAAATAGCACCATCACAGTAACCATGAAGGT GTTCAAGAGACGGTATTTTTATTTGACTCAACTTCCTGATGGTTCATATATTCTCAATTCTTATAAAGAT GAGAAAAATTCCAAAGAGTCTAAAGGTTGCATCTACTTGGATGCCTGCATTGATGTTGTTCAGTGCCCCA AAATGCGCCGTCATGCTTTTGAACTCAAGATGTTAGATAAGTATAGCCATTATCTGGCAGCTGAAACGGA GCAGGAAATGGAGGAATGGTTGATAATGTTGAAAAAGATTATTCAGATCAATACTGACAGTTTAGTGCAA GAGAAAAAGGACACAGTCGAGGCAATACAAGAGGAGGAAACTAGCAGCCAAGGAAAAGCCGAGAACATCA TGGCCAGTTTGGAAAGGAGCATGCATCCGGAACTGATGAAGTATGGCAGAGAAACAGAACAACTGAACAA ACTCAGTAGAGGAGACGGAAGACAGAATCTCTTTTCTTTCGACTCAGAGGTTCAGAGATTAGACTTTTCA GGAATTGAGCCTGATGTAAAGCCATTTGAAGAAAAGTGCAACAAGCGTTTCATGGTGAATTGCCATGATT TAACTTTCAATATCTTGGGTCACATTGGAGACAATGCAAAAGGACCACCCACAAATGTTGAGCCCTTTTT CATCAACCTCGCCTTATTTGATGTAAAGAACAATTGTAAAATTTCAGCAGACTTTCATGTAGATTTGAAC CCACCCTCTGTCCGTGAAATGCTGTGGGGTACCTCGACCCAACTGTCCAATGATGGGAATGCAAAGGGCT TTTCACCTGAGTCTCTCATCCATGGAATTGCTGAGTCTCAGTTATGCTACATAAAGCAGGGAATCTTCTC AGTGACGAACCCACATCCTGAAATTTTCCTAGTTGTGAGAATTGAAAAGGTGCTGCAGGGAAACATCACA CACTGTGCAGAACCCTACATCAAAAACTCAGATCCGATAAAGACAGCCCAGAAGGTACACAGGACAGCTA AACAAGTGTGTAGTCGCCTTGGACAATACAGAATGCCATTTGCTTGGGCAGCCAGACCCATTTTCAAAGA TGTTCAGGGCTCTCTGGATCTGGATGGGAGATTTTCCCCTTTGTATAAACAAGACAGTAGCAAGCTTTCA AATGAAGATATTCTCAAGTTGCTCTCAGAATACAAAAAGCCAGAGAAGACAAAATTGCAGATTATCCCTG GACAGCTGAGTATCACAGTGGAATGTGTTCCTGTAGATTTACCAAATTGTATTACCTCTTCATATGTGCC CCTGAAGCCTTTTGAAAAGAATTGTCAGAATATTACTGTGGAGGTTGAAGAGTTTGTTCCAGAAATGACA AAATATTGTTATCCATTCACTATTTACAAAAATCATCTGTATGTATATCCCTTGCAATTAAAATATGACA GCCAGAAATCGTTTGCCAAGGCTAGGAACATTGCTGTCTGTGTGGAATTCCGGGATTCGGATGAAAGTGA CGCGTCTGCGCTAAAGTGCATTTACGGAAAACCAGCAGGGTCTGTTTTTACCACAAATGCTTATGCTGTG GTCTCGCATCACAACCAAAATCCGGAGTTCTATGATGAGATCAAAATTGAGCTTCCCATTCACCTGCACC AGAAGCATCACCTGCTTTTTACTTTTTATCATGTGAGTTGTGAAATCAACACAAAAGGAACAACCAAAAA GCAAGACACAGTGGAAACTCCAGTGGGATTTGCCTGGGTACCTTTGCTGAAGGATGGGAGGGTCATCACA TTGGAGCAGCAGCTGCCAGTCTCAGCCAATCTTCCTCCAGGCTACTTGAATGTGAATGATGCTGAATCAA GAAGGCAATCAAATGCGGATATCAAGTGGGTAGATGGTGCAAAGCCTTTGCTGAAGATTAAGACCCACTT AGAATCAACCATTTATACTCAGGATCTGCATGTGCACAAATTCTTCCATCATTGCCAGCTGATTCAGTCG GGCTCAAAAGAAGTTCCAGGGGAACTCATTAAATATTTAAAGTGTTTGCATGCCATGGAGATCCAAGTCA TGATACAGTTTCTACCTGTAATTCTTATGCAACTCTTCCGAGTTCTCACAAACATGACCCATGAAGATGA CGTTCCCATCAACTGCACCATGGTTCTCTTACACATTGTGTCTAAGTGTCATGAAGAAGGCTTGGAGAGC TACTTAAGATCCTTCATAAAGTATAGCTTCAGACCTGAAAAACCAAGCACTCTTCAGGCCCAGTTGATAC ATGAAACCCTGGCTACGACTATGATAGCAATATTGAAACAGTCTGCAGATTTCCTAGCCATAAATAAACT TCTAAAGTACTCATGGTTTTTCTTTGAAATAATTGCAAAGTCGATGGCCACATACTTATTGGAAGAGAAT AAAATTAAGCTCCCCAGGGGCCAAAGGTTTCCTGAGGCGTATCACCACGTCTTACATTCTCTGCTCCTTG CAATAATTCCCCATGTGACCATTCGCTATGCAGAGATTCCTGATGAGTCCCGAAATGGGAATTATAGCTT AGCCAGCTTTCTGAAGCGCTGCTTGACCCTGATGGACAGAGGATTTGTTTTCAATTTGATAAATGACTAT ATATCAGGATTTAGCCCTAAGGATCCTAAGGTTCTTGCTGAATACAAGTTTGAATTTCTCCAAACAATCT GCAATCATGAACATTACATTCCTCTGAACTTGCCAATGGCATTTGCAAAGCCTAAATTACAGAGAGTTCA AGATTCCAATCTTGAATACAGTTTATCAGATGAGTATTGCAAGCATCACTTCTTGGTTGGCCTACTTCTG CGGGAAACCTCCATTGCTCTTCAAGACAACTATGAGATCAGATACACAGCTATCTCAGTCATAAAGAATC TTTTGATAAAACATGCATTTGATACTAGATACCAGCACAAGAACCAGCAAGCCAAAATAGCGCAGTTGTA CCTCCCATTTGTCGGACTGCTCTTGGAAAATATCCAGCGACTGGCAGGCCGAGATACTCTGTATTCCTGT GCAGCCATGCCTAGTTCTGCATCTAGGGATGAGTTTCCATGTGGCTTTGTTTCACCTACAAACAGAGGGA GCCTGGCCAGTGACAAAGACACAGCCTATGGGTCATTTCAAAATGGTCATGGAATTAAGAGGGAAGATTC AAGAGGTTCCCTTATTCCTGAAGGAGCAACAGGATTTCCAGACCCTGGGAGCACTAGTGAAAACACACGA CAGAGTTCTTCAAGGAGTAGTGTATCCCAGTATAATCGTCTGGATCAGTATGAAATCAGAAACCTCTTGA TGTGCTACTTATACATAGTAAAGATGATTTCTGAAGATACTCTCTTGACTTACTGGAATAAAGTGTCTCC TCAGGAGCTCATAAACATTCTTGTACTTCTAGAAGTATGTTTGTTTCACTTCCGATATATGGGGAAAAGA AACATAGCAAGGGTACATGATGCCTGGCTATCCAAACACTTTGGAATAGATAGAAAATCACAAACTATGC CAGCTCTTCGAAACAGATCAGGGGTGATGCAAGCTCGGCTTCAACATCTCAGCAGCTTAGAAAGCTCATT CACACTTAATCACAGTTCTGCAACAACGGAAGCAGACATTTTCCACCAGGCGCTTCTGGAAGGCAATACT GCTACTGAAGTATCTCTGACAGTACTAGACACGATCTCCTTTTTTACCCAGTGCTTCAAGAACCAACTTT TAAATAATGATGGTCACAACCCACTAATGAAAAAAGTATTTGATATTCACCTTGCTTTTCTTAAAAATGG ACAATCTGAAGTGTCACTGAAGCATGTCTTTGCCTCACTGAGATCTTTCATCAGTAAGTTTCCCTCAGCC TTTTTCAAAGGAAGAGTAAACATGTGTGCTGCATTTTGCTATGAGGTTTTAAAGTGCTGTACTTCAAAGA TTAGCTCAACCAGGAATGAGGCATCTGCACTTTTGTATCTTTTGATGAGAAACAACTTTGAATATACCAA GAGGAAAACCTTTCTGAGGACACACTTGCAGATAATCATTGCTGTGAGTCAGCTAATAGCTGATGTAGCA CTAAGTGGAGGATCGAGATTTCAAGAGTCTTTATTCATTATCAATAACTTTGCAAATAGCGACAGACCTA TGAAGGCAACTGCTTTTCCCACAGAAGTCAAAGATTTGACCAAGAGGATCCGCACCGTTCTTATGGCCAC TGCCCAAATGAAGGAACATGAGAAAGACCCTGAAATGCTAATTGATCTCCAGTATAGCTTAGCTAAATCT TATGCCAGCACACCAGAGCTGAGGAAAACTTGGCTTGACAGCATGGCCAAAATTCATATAAAAAATGGAG ATTTTTCTGAGGCTGCAATGTGTTATGTCCATGTAGCGGCTCTAGTTGCAGAATTTCTTCACCGAAAGAA ATTATTCCCTAGTGGGTGTTCAGCGTTCAAGAAAATTACTCCCAATATAGATGAAGAAGGAGCCATGAAA GAAGATGCCGGGATGATGGATGTTCATTACAGTGAAGAGGTTTTGCTGGAATTGCTAGAACAGTGTGTGG ATGGTTTGTGGAAGGCAGAGCGATATGAAGTAATTTCTGAGATTTCCAAGTTGATTATTCCAATTTATGA GAAACGCCGTGAATTTGAGAAACTGACTCAAGTTTATAGAACTCTCCATGGCGCTTACACAAAAATTTTG GAGGTTATGCACACCAAAAAACGACTTTTAGGTACTTTCTTTAGAGTTGCCTTTTATGGCCAGTCTTTTT TTGAAGAAGAAGATGGAAAGGAGTATATCTATAAGGAACCCAAGCTCACTGGCCTTTCAGAGATTTCCTT GAGACTTGTGAAACTTTACGGGGAGAAATTTGGTACTGAAAATGTCAAAATAATTCAGGATTCAGACAAG GTAAATGCCAAAGAGCTTGATCCAAAATTCGCTCATATACAAGTCACTTATGTGAAGCCGTATTTTGATG ACAAAGAACTCACAGAAAGAAAGACGGAGTTTGAAAGAAATCATAATATCAACAGATTTGTTTTTGAGGC CCCATATACATTATCAGGCAAGAAACAAGGTTGCATTGAAGAACAGTGCAAGCGTCGTACTATCTTGACC ACTTCAAACTCATTTCCATATGTAAAAAAGAGGATACCTATAAACTGTGAACAGCAGGTGAATTTAAAAC CAATTGATGTTGCTACTGATGAAATCAAAGATAAAACTGCAGAGCTACACAAGCTCTGCTCCTCTGTTGA CGTGGACATGATTCAGCTTCAGCTAAAGTTGCAGGGCTGTGTCTCTGTGCAGGTCAATGCTGGTCCATTA GCATATGCGAGGGCTTTCTTAAATGAAAGTCAAGCTAACAAGTATCCACCTAAAAAAGTGAATGAGTTGA AGGACATGTTTAGAAAATTCATACAAGCATGCAGCATTGCACTTGAACTAAATGAGCGGCTAATTAAAGA GGATCAAATTGAGTACCATGAAGGGCTAAAGTCAAATTTCAGAGACATGGTAAAAGAACTGTCTGACATT ATCCATGAGCAGATACTACAAGAAGACACAATGCATTCTCCCTGGATGAACAACACATTACATGTATTTT GTGCAATTAGTGGTACATCAAGTAACAGAGGTTATGGTTCCCCAAGATACGCGGAAGTATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001009947
Insert Size	6222 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001009947.3, NP_001009947.2
RefSeq Size	6665 bp
RefSeq ORF	6222 bp
Locus ID	75974
UniProt ID	A2AF47
Gene Summary	Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP (PubMed:15710388, PubMed:16968698, PubMed:25851601). Required for marginal zone (MZ) B-cell development, is associated with early bone marrow B-cell development, MZ B-cell formation, MZ B-cell number and marginal metallophilic macrophages morphology (PubMed:25729399). Facilitates filopodia formation through the activation of CDC42 (PubMed:22494997).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.