Ankrd12 (NM_001025572) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2900001A12Rik; AI447928; ANCO-2; AV347965; GAC-1; mKIAA0874
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225145 representing NM_001025572 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAAATCTGGGTTCACAAAACCAGTTCAGAGTGAAAATTCTGACAGTGACAGCAGTATGGTGGAGA GGCCATATGGAAGAAAGAGTAAAGACAAGATTGCATCTTACAGCAAAACTTCAAAAATTGACCGAGGTGA TATGGGCAAGGAAATGAGAGAGAAACCATCCATGAAACGAAAGCTGCCTTTTACAGTCAGCCCATCGAGA AATGAGGAGCGCGACTCAGACACAGATTCAGACCCAGGACATACAAGTGAAAATTGGGGGGAGAGACTTA TATCTTCTTACAGGACATACTCAGAAAAAGAAGGTCCAGAGAAGAAGAAAACAAAAAAAGAAGCTGGAAC TAAGAAGTCCACCCCAGTTAGCATTCTTTTTGGTTATCCACTTTCTGAACGAAAGCAGATGGCACTCCTA ATGCAGATGACAGCAAGGGACAACAGCCCAGATTCCACACCAAGTCATCCGTCACAGGCAACGCCAGCCC AGAAGAAAACGCCCAGCTCTTCATCTCGACAGAAAGATAAAATTAATAAAAGAAATGAGCGTGGCGAAAC TCCTTTACACATGGCCGCTATTCGAGGAGACGTGAAGCAAGTCAAAGAGTTAATTAGTTTAGGGGCAAAC GTCAATGTGAAGGACTTTGCAGGTTGGACCCCTTTGCATGAAGCTTGCAATGTTGGCTATTACGATGTTG CTAAGATACTGATAGCAGCTGGCGCAGACGTTAACACACAAGGACTGGATGATGACACCCCGCTCCATGA CTCTGCCAGCAGTGGCCATAGAGACATAGTGAAGTTGTTGCTTCGTCATGGCGGGAATCCATTCCAAGCT AATAAACATGGAGAACGGCCAGTGGATGTGGCAGAGACAGAGGAGTTGGAGTTGCTGCTAAAAAGAGAGG TGCCTTTGTCTGGCGATGATGAAAGTTATACAGATTCAGAAGAGGCTCAGTCTGTAAATCCTTCTAGTGT TGATGAAAATATTGACTCAGAGACAGAGAAAGACTCTTTCGTCTGTGGCAGTAAAATCCTCCCAAGCAAA GCCCCGCTGCCTTCTGCACTGGATGAGTATGAGTTCAAAGATGATGATGAAGAAGAGATAAGTAAGATGA TTGACGACAGGCACATTCTTAGGAAAGAACAGCGGAGAGAGAATGAATCTGAAGCAGAAAGGAGTGGTCT GTTTGCAAAACAGGAGAAAACCTTCTATTCTAAGTCATTTAAAACAAAAAAACAGAAGCCGTCAAGGGTT CTGTGCTCCAGTACTGAAAGCTCTGATGAAGAGGGTCTTCAGAACAGAAAGATTTCTACTGCTTGTTCTG TTGCTGAATCATCACATCCTGATATGCAAGCCAAAAAAGAATATGAATACAAACAGAAAGGCAAAGTTAA AAGAAAATTAAAAAATCAGAATAAAAACAAAGAGAACCAAGAGCTGAAACAAGAAAAAGAGGGGAAAGAA AATAGCAGGATAGCAACCTTGACAGTTAATACTGCGCTGGATTGTTCAGAAAAATACAGAGAGGAGGGGA ACTTCAGGAAATCTTTTAGCCCAAAGGATGATACTTCATTACACTTATTTCATATTTCCACTGGTAAATC TCCCAAACATTCCTGTGGATTAAGTGAAAAACAGACACCACTGAAGCAAGAACACACGAAAGCATGCATA TCCCCAGGAAGTTCTGAAATGTCATTACAGCCTGATCTCACTCGGTATGATAATGCAGAACCTGAAAGTT CAAATGTCAAATCTTATAAGCACAAGGAAAAAAACAAACAGCAGAAAGATTTTCACATAGAGTTTGGTGA AAAATCAAATGCCAAAATTAAAGATGAAGATCATAATCCAGCATTTGAAAACTCAGACTGCACACTGAAG AAGATGGATAAAGAGGGTAAAACATTAAAAAAACATAAATTAAAACATAAAGAGAAGGATAAAGAAAAGC ACAAAAAGGAAGTTGAAGGTGAAAAGGAAAAATATAAAAATAAAGACAATGCTAAAGAGCTACAGAGGAG CATCGAATTTGACAGAGAGTTTTGGAAAGAAAATTTTTTTAAAAGTGATGAAACTGAGGATCTCTTTTTA ACTATGGAACATGAGTCCATAATAGAAAAAAAGACAAAAATGGAAAAAAACATGAAAGATGATAGGTCAG CTAAAGAAAAGCATGTCTCAAAAGAAAGGAACTTTAAAGAAGAACGGGAAAAGACTAAAAAGGAAAATGA AAAATCTCTTAGGGAAGAAAAGGTAAAAGATCCGAAAGAAGAAAAAGACAGTGTATCCACAGAGAAGGAG GCCGAGTGCTGTTCGGTGGGTGTCAGTGTGAGCCAGGAAGCTGCAGGGCTGCATTCATCAGAAAAGGAAA TGGATGCTGAGAAGCAAGAAAGACACACAAAAGAGAGGGAAAAGTCTGATCGGCGCTTTCAGACTAAGGA GAAAGAGCTTGACAGGAACGAGAGGAAATTTTCTGAAAAAGAAAAGAAGATAAAACATGAACATAAGTCA GAAAGAGAGAAGCTGGATTTCAGTGACTGCACTGACAGAGTGAGAGAGAAGGACAAGCTGTACTCACACC AGACAGAAAGGTGCCACAAGGACAGCGAGAAGCTGAAAAACACTGTCAGAAAGACTGATGACAGAGAGAG GAACAGAGAAAAGGCGGATAGAAAGCACGACAGAGAGAAACCTGAACGAGAGAGGTACTTAGCAGAAAAC AAAGAAAAGCACTTAGAGAAAAGAAAGCAATCTGACAGCAGTGAGTATTCCAAATCAGAGAAGGTCAGAA CTAAAGACAGAGACAGGGAAGGAGAGAAGAAGGAGAAACTTAGAGATAAAGAAAGTGTAAATGTAACTAA CTTGAAACATTTGCAGGAAGAGAGAAAGTCCAGTACAGGAGACAGCAGTGGTAAAACCCAGCATGAGAAA GCTCTGTCCCTTAAAGAAAGGGCAAGAGATGAGCCTTTGAAAACTCCAGATGGAAAAGAGAAAGATAAAA AGGATATAGACAGATACAAAGAGCGAGACAAGCGTAAAGATAAAGCCCAGCTAAATACTTTAATCAGACT AAAATCTGAAACAGAGAAGCTTAAACCTAAATTATCACCAGCATCAAAAGATACTCGACCTAAAGAAAAG AGGTTAGTAAATGATGATTTAATGCAGACAAGTTTTGAACGAATGCTTAGCCTTAAAGACCTAGAAATAG AACAGTGGCACAGAAAACATAAGGAAAAAATTAAGCAAAAGGAAAAAGAACGATTACGCAATCGTACTTG TTTAGAACTTAAAGTAAAAGATAAAGAAAAAACAAAACATGCACCCACAGAATCCAAAAGTAAAGAACTT ACCAGGTCAAGAAGTTCAGAGTTGACTGATGTTTATAGCAAGGAGAAACAATCTAAAGATGTTGGCAGTA ATCGCTCACAATCTGTTGACAACAGAAATGTATTGAATTTAGGAAAGCCATCCTACATTTCAGAGAGTGG CTCCAACCGGTCTCCTAGGTCGGAAGGTGAGAGGCTGGGTCTTAGCTCTAGGTCTGTGTCCATGCTTTCT GTTGCTAGTTCAGAAGACTCTTGCCATACAGTGACAACCCCAAGGCCTCTCGTTGAGTATGACTCTGACT TTATGTTAGAGGGCTCGGAATCTCAGTTGTCATTTTCTCAGTCACCTTTTTTGCCAAATGCCAAATCTCC AGCTCTTTATGAAAGAGAGCTGGAGGGTCTCACTGAACTACCAGACCGACTTAAACCACCATATACAAAC AGACTTCCAGTGTGCCATCTCAGGTCATCTTCTGTGGAAGATGTTAAACTAATTACTAATGAGGTAAGAC CTGCTGTAGAAATTAGAAGATGCAGTATGCCCTCAGTTATCTGTGAGCCCATAAAACATTTCCAAGTATC AGAAGAAAGCAGTCAAGGCAACTTAGCTGTTCCCAGAGAGACCTGTCTGTCTCCCAAGCCTGAAGTGTCT TCAGCAGTACCTGAGAGGGGCCTTTCGCATGTGTCTGACTTACATCCTAACTGTACAGGCTCTCCAACGA GGTCTGCAAACAGCAGCTATATTTCATCTGATGTAAATGTAATCAAGAGTGCAACTCTTGTGGGTACACT GATTGACAGTTCGATGCATTTGGAACCGTCTAATCAGGTTGGAGTGATTCCAAGTAAATCATGGGAGACG CCTGTTGATAAACTGGAGTCTTTAAGCACCAATGACTTCATCTGTCCAGATTCTAATATATCTGATCAAG ATTCCTCTGCTCAGAGTTTTTATAATTCAGAAAACAAAATGTTAAGAGAACAAAATACTGACTGCTTACC TCCGCACCAGCCTGAACTGCCAGGAAACTCATGTGCTCAGGACCCAGCATCCTTTCTGTCTTCACCTCAG CCCTGCTCTTTTTCCAACCAACCACTGGCAGGTGCTGACTCTGTTCCGAAACCCGTGTCTTTGTCATATG TTGTCAGTCAAGAGCCAGGTGTTTTACAACAGAAAAGTGCAGCTCAGATGGCCAGTTCTGTTTTAGATAG TGATAATAACTTTCCAGAGGATGTGGAAAATACATTTGTCTTAGCAGACATTCAGAAGACAAATTCTTTT TTACCAGCTTACTCTGAAAGCACTGTGCAAGAAGCACTCCCAAGCTTTGAGAAGGCCAATACTTTGCTTG TGTTGCCATCAGAAAAGGACTTCAATGGGAGTGATACTGCTCAGCCAAACACACATTATGCATTTAGCAA ACTCACATACAAGTCTTCCAATAGCCAGGAAATTGAGAAGGGCCCAGATGATACTCAAATCATTTCACAT GAAAAGGAAAACAAATTACAAAGTTTGGTCTTAACTCAGTTGAGTAAATGTGATTCTGATTTGTATGAGA TGAATGCAGGGCTGCCAAAAGGAAACCTGAATGAACAGGATAACCCAAAGCATGGTCTTGACAGCGAAAA GTACTCGCTTTCCACTGAAGATGAAGAATCTCAGCAGAGCACCTTATCAAGTGTGGAAAACCATTCACAG CAGTCGGCCCAACCAGAGATGCATAAATATGACCAGCTTAGTAAAGTAGAACTAGAAGAAAATGCCGAAG ACGATAAAACTGAAAACCAGACCCCTCAAAGAGTGACTAGAAACAAAGCAAGCACAGTGGTCAATCCAAG CAAGCCCATGCTTGCTGGCTGTGCCCTTGTAGCAGAGAAAGATAACGAGTCCTCCCCTAGGGGACGAATC AGACTCACTGAAGAGGATGATCCTCAGATCCACCATCCTCGGAAGAGGAAAGTGTCACGGGTTCCTCAGC CTGTGCAAGTGAGCCCCTCTTTACTGCAAGCAAAGGAGAAAACTCAGCAGTCTCTAGCAGCCATTGTAGA TTCTCTGAAGTTAGATGAGATTGAGCCATACAGTTCTGAGAGAGCAAATCCATATTTTGAATATTTGCAC ATAAGAAAAAAAATTGAAGAAAAACGCAAGTTGCTATGTAGCGTTATCCCTCAAGCTCCTCAGTATTATG ATGAATACGTAACGTTTAATGGATCCTATCTCCTGGATGGAAACCCCTTAAGCAAGATTTGTATTCCTAC AATCACACCACCACCTTCGCTGTCAGATCCACTTAAAGAGCTCTTTCGGCAACAGGAAGTAGTAAGGATG AAACTGCGTTTGCAACATAGTATTGAAAGGGAAAAACTTATTGTATCCAATGAACAAGAAGTTCTGCGAG TTCATTACAGAGCTGCAAGAACACTGGCCAATCAAACACTGCCATTTAGTGCTTGTACTGTCTTACTGGA TGCAGAAGTGTACAATGTGCCCTTGGACTCACAGTCTGATGACAGCAAAACTTCTGTGAGAGATCGATTT AATGCAAGACAGTTCATGTCCTGGTTGCAAGATGTGGACGATAAATTTGACAAGCTAAAGACCTGTCTCT TAATGAGGCAGCAACACGAAGCTGCAGCTTTAAATGCCGTCCAGAGGCTAGAATGGCAGCTCAAACTCCA GGAGCTGGACCCTGCCACGTATAAGTCTCTCAGTATTTATGAAATCCAGGAGTTTTATGTTCCCCTTGTT GATGTTAATGATGATTTTGAACTGACTCCTATATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001025572
Insert Size	6126 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001025572.1, NP_001020743.1
RefSeq Size	8991 bp
RefSeq ORF	6126 bp
Locus ID	106585

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