Lrrk1 (NM_146191) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW319595; C230002E15Rik; D130026O16Rik; mKIAA1790
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225125 representing NM_146191 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGCACGTCACAGCGGCCACCCAGTATGTACTGGTGTGTGGGGACAGAAGGATTAGCTGTGTGTC CAGGACCTGCCATGGAGACCCATAATGGTGCCGAAGACATGGGCAGCAAACTGTCTTTACCAGGTGGCAG CTCCACGGTGCAGTGCCCCAGCATGGAAGAAATCCACACTGCGTACAAGCAGAGGAACCTCTCCCGAGCC CGAGACCTACTCAGAGGGGTTTGTGAAGAGAGCGAATCCTCACAGGAGAAGGGCCAGCTGCTGAGCATCG CGGCAGCCCACGGGGATCTGGAGACTGTTCAGTTCCTGCTCACTGAGAAGCGCGTGGAGCTGCCGACGGA GCCCACCGATGACAACCCAGCTGTGGTGGCAGCGCATTTTGGACACGCCGAAGTAGTACGTGAACTACTA GAGTCCTTGCCAGGTCCCTGTACCCCCCAGCGGCTACTGAACTGGATGCTGGCCCTGGCTTGCCAGCGAG GGCACCTGGAGGTTGTGAAGCTGCTGGTCCTGACACATGGGGCTGACCCGGAGAACTATGCCGTCAGGAA GAATGAGTTCCCCGTCATTGTGCGGTTGCCCCTCTATGCGGCCATCAAGGCAGGGAATGAAGACATTGCC ATATTCCTGCTTCGGCACGGAGCCTATTTCTGCTCCTACATCCTGCTGGATAGTCCTGAGCCAAGCAAAC ATCTGCTCAGGAAGTATTTCATCGAAGCCAGCGCCCTGCCCAGCAGCTATCCCGGGAAAATCGCACTTCG TGTGAAATGGTCCCATCTGAAGTTGCCCTGGGTGGACCTGGACTGGCTCCTAGACATCTCCTGCCAGATC ACAGAGCTTGACCTGTCTGCCAACTGCCTGCCTTCGCTCCCTTCCATCATCCCTTGGGGACTCATCAATC TAAAGAAGCTGAATCTCTCAAACAACCAGCTGGGGGAGCTGCCCTGCGTGCAGTCCTCCGACGAAATCAT CTGCTCCAGGTTACTTGAAATTGACATCTCCAGCAACAAACTGTCTCACCTCCCTCCTGGATTCCTGCAC CTCTCAAAACTTCAAAAGCTGACTGCTTCCAAAAACTACCTGGAGCGGCTGTTTGAAGAAGAAAACGCCA CTAACTGGATCGGCCTGCGGAAGCTGCAAGAACTCGATCTAGCTGACAACAGGCTGACAGAGCTCCCTGT CCAATTTATGCACTCCTTCAAGTCTCTCACCTCTCTGAATGTCTCCAGGAACAACCTGAAGAGCTTCCCG GATCCCTGGTCCTGCCCTTTGAAATGTTGTAAAGCCTCCAAAAATGCCCTGGAATCGCTGCCAGACAAAA TGGCTGTCTTTTGGAAAAGCCACCTTCGGGATGCAGATTTCTCTGAGAACTCTCTGAAAGAGGTGCCCCT GGGATTGTTCCAGCTTGACGCCCTCATGTTCTTGAGGTTACAGGGAAACCAGCTGTTGTCGCTGCCACCT CAAGAGAAGTGGACCTGCACCCAACTCAAGACCCTGGACCTCTCCAGAAACCAGCTCGGCAAAAATGAAG ATGGGCTTAAAACAAAGAGGATCTCCCTGTTCACCACCAGAGGGCGCCAGCGTTCTGGAACTGAGACAGC CTCCATGTTGGAATTCCCAGCTTTCCTAAGTGAGTCTTTGGAGGTCCTTTGTCTGAATGACAACCATCTT GATGCTGTTCCCCCATCGGTCTGCCTGCTGAAGAACCTCTCAGAGCTCTACTTGGGCAATAACCCTGGCC TCCGAGAGCTCCCTCCGGAGCTAGGCCAGCTGGGGAACCTCTGGCAACTGGACATTGAAGATCTGAACAT TAGCAACGTGCCTGCGGAGGTCAGGAAAGAAGGCCCGAAAGCAACACTGTCTTTCCTGCGTGCTCAGCTG CGCAAAGCAGAGAAGTGTAAGCTGATGAAGATGATCCTCGTGGGTCCCCCGCGCCAGGGCAAGTCCACAC TCCTGGAGATCTTACAGACCGGGAAGGCCCCGCAGCTAGCACACAGTGAAGCCACCATCAGGACCACCAA GTGGGAGCTCCAGAGACCAGCAGGCTCCAAAGCCAAGGTTGAGTCCGTAGAGTTCAACGTCTGGGACATC GGGGGTCCGGCCAGCATGGCCACGGTCAATCAGTGCTTCTTCACAGACAAGGCGCTGTATGTGGTGGTCT GGAACCTGGCACTGGGAGAGGAAGCCGTGGCCAACCTCCAGTTCTGGCTGCTCAACATTGAGGCCAAGGC CCCAAATGCCGTTGTGCTGGTGGTCGGGACACACCTGGACCTAATCGAAGCTAAGTTCCGAGTGGAGAGG ATAGCAACCCTGCGTGCCTACGTGCTGGCCCTCTGCCGCTCGCCATCGGGCTCCAGGGCTACGGGCTTCC CGGACATCACTTTCAAACACTTGCATGAGATCTCCTGCAAGAATCTGGAAGGGCAGGAGGGGCTGCGGCA ACTGATCTTCCACGTCACGTGCAACATGAAGGATGTGGGTAGCACTATCGGCTGTCAAAAACTCGCCGGC AGGCTGATCCCCAGGAGCTACATAAGCCTACAGGAGGCCGTGCTGGCTGAGCAACAGCGCCGCAGTCTGG GTGACCAAGTACAGTATCTGACGGACAGGCAGCTGGACCAGCTGGTAGAGCAGACGCCTGGCAATGATAT CAAAGACTATGAAGACCTGCAGTCTGCCATCAGCTTCCTCATAGAAACTGGGACCCTGCTGCACTTTCCA GACACGAGCCATGGCCTAAGGAACCTCTACTTCCTGGATCCCATCTGGCTCTCTGAATGCCTGCAGAGGA TCTTTAACATCAAGGGCTCGCGGTCAGTGGCAAAGAATGGGGTGATCCAAGCGGAAGACCTCAGGATGCT GCTGGTGGGGACAGGCTTCACACAGCAGACTGAGGAGCAATACTTCCAGTTCCTTGCCAAGTTCGAGATT GCCCTCCCTGTTGCCAATGACAGCTACCTCCTACCACACCTCCTTCCATCCAAGCCTGGGCTGGACACCC ACAGCATGCGGCACCCAATGGCTAACACCATCCAGCGGGTGTTTAAGATGAGCTTCGTGCCTGTTGGCTT CTGGCAACGGTTCATAGCACGGATGCTAATCAGCTTGGCTGAGATGGACCTGCAGCTTTTTGAAAACAAG AAGAATACGAAAAGCAGGAACCGGAAAGTCACTATTTACAGTTTTACAGGGAGCCAGAGAAACCGCTGCA GTACATTCCGAGTCCGAAGAAATCAGACCATCTACTGGCAGGAAGGGCTGCTGGTCACTTTCGATGGGGG CTACCTCAGTGTGGAATCCTCAGATGTGAACTGGAAAAAGAAAAAAAGCGGAGGAATTAAAATCATCTGC CAGTCAGAGATGAGGGACTTCTCGGCAATGGCTTTTATCACAGACCATGTCAACTCCCTGATTGACCAGT GGTTCCCTGCCCTGACGGCCACAGAAAGTGACGGGACCCCACTGATGGAGCAGTACGTACCCTGCCCTGT CTGTGAAGCATCCTGGGCCCAGCATGCAGATCCAAACGAGAGGTCAGAGAGCGTGCAGTACTTTGATATG GAAGACTGTGTGCTCACAGCCATCGAACGGGACTTCATCTCCTGCCCCAGACACCCAGACCTCCCAGTAC CACTTCAGGAGCTTGTACCAGAGCTGTTCATGACTGACTTCCCAGCCAGGCTCTTCCTGGAGAACAGCAA GCTGGAGCACACGGAGGGTGAGAACAGCATTCTGGGCCAAGGTGGCAGTGGCACAGTCATCTACCAGGCC CGGTACCAGGGCCAGCCTGTGGCTGTGAAGAGGTTCCATATCAAGAAGTTCAAGAATTCTGCTAATGCCC CAGCAGACACCATGCTGCGGCACCTGAGAGCCATGGATGCCATGAAGAACTTCTCAGATTTCCGTCAGGA GGCCAGCATGCTGCATGCCTTGCAGCATCCTTGCATCGTGTCACTCATCGGCATCAGCATCCACCCGCTC TGCTTCGCCTTGGAGCTGGCCCCGCTGGGCAGTCTCAACACCGTGCTATCTGAGAATGCCAAAGATTCTT CCTTTATGCCCCTGGGACACATGCTCACCCAAAAGATAGCCTACCAGATTGCCTCGGGCCTGGCCTACCT GCACAAGAAAAACATCATCTTCTGTGACCTCAAGTCGGACAACATCCTAGTGTGGTCGCTGAGCGCCAAG GAGCATATCAACATCAAACTGTCAGACTACGGCATCTCCAGGCAGTCCTTTCACGAGGGGGCCCTGGGTG TGGAGGGCACCCCAGGTTACCAGGCCCCAGAGATCAGGCCTCGCATCGTGTATGACGAGAAGGTAGATAT GTTCTCCTACGGCATGGTGCTATACGAGCTGCTGTCAGGACAGCGTCCTGCACTGGGCCACCACCAACTT CAGATTGTCAAGAAGTTGTCCAAGGGCATCCGCCCAGTTCTGGGGCAGCCGGAGGAAGTTCAGTTCCATC GGCTGCAGGCGCTCATGATGGAGTGCTGGGACACAAAGCCGGAGAAGCGCCCCCTGGCCTTGTCCGTGGT GAGCCAGATGAAAGACCCAACCTTTGCCACCTTCATGTACATGCTGCCCTGTGGGAAGCAGTCGGCCTTC TTCTCATCCCAGAGCCAGGAGTACACTGTGGTGTTCTGGGATGGGAAGGAGGAGTCCAGGAATTACACGG TGGTCAACACAGAGAAGGGCCTTCTGGAAGTACAGAGGATGACCTGCCCCGGGATGAAGTTGAGCTGTCA GCTCAAGGTCCAGAGCTCAGTGTGGATAGCCACGGAGGACCAGAAAATCTATATCTACAGCCTTAAGGGT ATGTGCCCATTAAGCGTGCCCCAGCAGGCCCTGGACACCCCAGCTGTCGTCACCTGTTTCTTGGCAGTAC CTGTTATCAAAAAGAACTCCTTCCTGGTGCTGGCAGGCCTGGCTGACGGACTCGTGGCTGTGTTTCCTGT GGCACGGGGCACCCCAAAAGAAAGCTGCTCCTACCTGTGCTCCCACACAGCCAACAGGTCCAAGTTCTGC ATCCCCGACGAAGATGCACGGCAGAACCCTTACCCAGTGAAGGCAATGGAAGTGGTCAACAGTGGGTCTG AGGTCTGGTACAGCAACGGGCCTGGCCTCCTCGTCATTGACTGCACCATCCTGGACATCAGCAGGCGGCT GGAGCCCTACGCAGCTCCGTCCATGGTCACGTCACTTGTGTGCAGCTCAGACTGCAGAGGAGAGGAGATG GTCTGGTGCCTCGACGACAAGGCCAATTGCTTAGTGATGTACCACTCAGCCACCTACCAGCTCTGTGCCC GGTACTTCTGTGGGGACCCCAATCCTCTCAGGGACACGTTTTCTGTGCAGCCTTCAGTCCTAGAAACCCC AGGCAGTCACAAAACCACCTCAAAGGGGCCTGTGGAAGAGTGCATCGCGGATGTGAGCATCATGTACAGC GAGGAGCTGGGCACGCAGATCCTCACCCACCAGGACTCGCTCACCGACTACTGCTCCATGTCCTCTTATT CTTCGTCCCCACCCCACCAAGATCCTAGGTCCCCTTCCAGTCTGCCCAGCTCCCTCACCAGCTATTCCAG TGTGCCTTTCTCTGCCAACTATGAAGACTCAGACAGGCTGCAGGAGCCCAGTGTCACCTCTGACAGAACT GAGCATGACCTGAGCCCCATGGATGGGGAGACGTTCAGCCAGCACCTGCAGGCTGTGAAGGTGCTTGCTG TCAAAGACCTCATTTGGGTTCCTAGACATGGAGGAGATATCATTGTCATCGGCCTGGAGAAGGATTCAGG TGCCCAGCGGGGCAGAGTCATCGCTGTTTTAAAAGCCCGAGAGCTCAATCGGCACGGGGTTCTGGTGGAT GCCGCAGTGGTGGCAAAGGACACCGTAGTGTGCAGCTTTGCAAATGAAAACACAGAGTGGTGCCTGGCTG TCTGGAGGGGCTGGGGCGCCAGGGAGTTTGACATCTTCTACCAGTCCTATGAGGAGCTGGGCCGGCTGGA GGCTTGTACTCGCAAGAGAAGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_146191
Insert Size	6045 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_146191.3, NP_666303.3
RefSeq Size	7467 bp
RefSeq ORF	6045 bp
Locus ID	233328
UniProt ID	Q3UHC2

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