Tcf20 (NM_013836) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2810438H08Rik; mKIAA0292; SPBP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225087 representing NM_013836 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTCGTTTCGGGAGCAAAGCAGTTACCACGGAAACCAGCAGAGCTACCCACAGGAGGTGCACAGCT CATCCCGCATAGAAGAGTTCAGCCCTCGCCAGGCCCAGATGTTCCAGAATTTTGGGGGAGCAGGTGGTGG TAGTAGTGGCACTGGCAGCAGCAGTAGCGGTCGACGAGGAACAGCTGCTGCAGCGGCAGCAATGGCTAGT GAGACCTCTGGCCATCAAGGCTATCAGGGTTTCAGGAAAGAAGCTGGAGATTTTTACTACATGGCAGGCA ACAAAGACACAGTGGCAGCAGGAACCCCACAGCCTCCTCAGCGAAGGCCTTCTGGGCCTGTACAGAGCTA TGGACCTCCCCAGGGGAGCAGCTTTGGCAATCAGTATGCGAGTGAGGGTCATGTGAGCCAATTTCAAGCA CAGCACTCTGCCCTTGGTGGTGTGTCTCATTATCAGCAGGATTACACAGGGCCTTTCTCTCCTGGGAGTG CTCAGTATCAACAGCAGGCCTCTAGCCAACAACAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCA GCAACAGCAGCAGCAAGTACAGCAGTTGAGACAACAGCTTTACCAATCCCATCAGCCTCTGCCACAAACC ACTGGACAGCCAGCCTCTGGCTCATCGCATCTACAACCAATGCAGCGGCCCTCAACTCTGCCATCTTCTG CTGGTTATCAGTTAAGAGTAGGTCAGTTTGGACAGCACTACCAGTCTTCTGCTTCCTCCTCTTCCTCCTC CTCCTTCCCTTCTCCACAGCGTTTCAGTCAGTCTGGACAAAGCTATGATGGCAGTTACAGTGTGAATGCT GGATCTCAGTATGAAGGGCATAATGTGGGTTCTAATGCACAGGCTTATGGAACACAATCAAATTATAGCT ATCAACCTCAGTCTATGAAAAATTTTGAACAGGCAAAGATTCCACCAGGAAACCAGCAGGGGCAGCAGCA GCAACAGCAGCAACCACAACCTCAGCAACAGCAGCCGCAGCAACAGCAGCAACAGCAGCAGCAGCAGCAA CATCCTCCCCAGCATGTGATGCAGTACACAAATGCTGCCACCAAGATGCCTCTGCAAAGCCAGGTTGGGC AGTACAACCAGCCTGAGGTTCCTGTAAGGTCTCCCATGCAGTTCCACCAGAATTTCAGCCCTATTTCTAA CCCTTCTCCAGCTGCTTCTGTGGTTCAGTCTCCAAGCTGTAGCTCTACACCTTCTCCTCTCATGCAGAGT GGTGAGAATCTCCAGTGTGGGCAAGGCAATGTGCCCATGAGTTCCAGAAACCGAATTTTACAGCTAATGC CCCAGCTCAGTCCAACTCCATCAATGATGCCCAGTCCTAACTCTCATGCTGCAGGATTCAAAGGATTTGG ATTAGAAGGGGTGCCGGAAAAGCGGCTGACTGATCCTGGGTTGAGTAGTTTGAGTGCTTTGAGTAGTCAA GTGGCCAATCTTCCTAATACTGTCCAGCACATGTTACTTTCTGATGCTTTGACACCTCAGAAGAAGACGT CTAAGAGGCCCTCATCATCATCTAAGAAAGCAGATAGCTGTACAAACTCAGAAGGCTCCTCACAGCCTGA AGAACAACTAAAGTCCCCTATGGCTGAGTCATTGGATGGAGGTTGCTCTAGTAGTTCAGAAGATCAAGGT GAGAGGGTGAGGCAACTGAGTGGCCAGAGCACTAGCTCTGACACCACCTATAAGTGTGGCGCTTCAGAGA AAGCTGGCTCCTCACCAACACAAGGTGCTCAGAATGAGGCCCCTAGGCTCAGTACCAGTCCTGCAACTAG GGACGAAGCTGCCTCTCCAGGTGCTAAGGACACATCACTGTCATCTGAGGGGAACACAAAAGTCAATGAG AAGACAGTTGGGGTGATTGTCTCCCGAGAAGCCATGACAGGTCGGGTAGAAAAATCTGGTGGACAAGATA AAGGCTCCCAGGAGGATGATCCTGCTGCCAGTCAGAGACCACCTAGCAATAGTGGCGTAAAGGAAATCAG CCACACATCACTTCCACAGCCAGATCCTCCAGGAGGAGGGAGCAAAGGAAACAAGAATGGTGATAATAAT AGCTCTAACCACAATGGAGAGGGGAATGGCCCGAGTAGCCACTCCGCAGTAGGCCCAAGTTTCACAGGCA GGACTGAGCCTAGCAAGTCTCCTGGAAGTTTGCGCTATAGTTACAAAGAGAGTTTTGGGTCAGCTGTACC ACGAAATGTCAGTGGTTATCCTCAGTATCCTTCAGGACAAGAAAAGGGGGATTTTGGCAGCCATGGAGAG CGAAAGGGTAGAAATGAGAAGTTCCCAAGTCTTCTACAGGAAGTGCTTCAGGGCTACCACCACCATCCTG ACAGAAGGTATCCTAGAAGCGCACAGGAACATCAAGGGATGGCTAGTGGCCTGGAAGGAACTGCAAGGCC TAACATCTTAGTCAGTCAAACCAATGAATTAGCTAGCAGGGGCCTTCTGAACAAGAGTATTGGATCCCTG TTGGAAAATCCCCACTGGGGACCATGGGAAAGGAAGTCAAGCAGCACAGCTCCTGAAATGAAACAGATCA ATTTGTCTGACTATCCCATTCCCAGGAAGTTTGAGATAGAACCTCCATCATCAGCCCATGAGCCTGGAGG CTCCCTTTCTGAAAGGAGGTCAGTAATCTGTGATATATCCCCACTAAGACAGATTGTCAGGGACCCAGGG GCTCACTCATTGGGACACATGGGTACTGATGCCAGAATTGGGAGGAATGAACGTCTCAACCCAAGTTTAA GTCAGTCAGTCATTCTTCCTGGTGGGTTGGTGTCCATGGAAACAAAATTGAAATCCCAGAGTGGACAGAT AAAAGAGGAAGACTTTGAACAATCCAAATCTCAAGCTAGTTTCAACAAGAAATCTGGAGACCACTGCCAT CCTACCAGCATCAAACATGAGACTTACCGTGGCAATGCAAGTCCTGGAGCAGCAGCCCATGATTCCATTT CAGACTATGGCCCACAAGATAGCAGGTCCACACCAATGCGGCGGGTCCCTGGTAGAGTTGGTAGCCGGGA GACTATGAGGGGTCGGTCCTCTTCTCAGTACCATGACTTTGCAGAAAAATTGAAGATGTCTCCAGGCAGG AGCAGAGGCCCAGGGGGAGACCCTCATCACATGAACCCACATATGACCTTTTCAGAGAGGGCCAATAGGA GTTCTTTACATGCTCCTTTTTCTCCCAACTCAGAAAGCCTGGCCTCTGCTTACCACACAAACACCAGGGC TCATGCTTATGGGGACCCTAATACCGGTTTGAATTCCCAGCTCCATTATAAGAGACAGATGTACCAACAG CAACAAGAGGAGTATAAAGACTGGGCCAGCAGTTCTGCTCAGGGAGTGATCGCTGCTGCGCAGCATAGGC AGGAAGGCCCACGCAAGAGCCCAAGGCAGCAGCAGTTTCTTGACAGAGTACGGAGCCCCCTGAAAAATGA CAAAGACGGAATGATGTATGGCCCACCAGTAGGTACGTATCATGACCCAAGCACTCAGGAAGCTGGGCGC TGTCTCATGTCTAGTGATGGTCTGCCTGCCAAAAGCATGGAATTGAAGCACAGCTCTCAGAAGTTACAAG AGTCTTGTTGGGATCTTTCTCGGCAGACTTCTCCAGCCAAAAGCAGTGGTCCTCCAGGAATGTCTAATCA AAAACGGTATGGGCCACCCCATGAGCCAGATGGACATGGACTAGCCGAGTCTGCACAGTCATCCAAACCT AGTAACGTCATGTTACGGCTTCCTGGTCAAGAGGATCATTCTTCTCAAAATCCTTTAATCATGCGAAGGC GGGTTCGTTCTTTTATCTCCCCTATTCCCAGTAAGAGACAGTCACAAGATGTAAAAAACAGTAACGCTGA TGATAAAGGGCGCCTCCTCCACCCATCAAAAGAAGGTGCCGATAAAGCATACAATTCCTACAGCCATCTT TCTCACAGTCAGGATATCAAGTCTATTCCTAAGAGAGATTCCTCCAAGGACCTTCCAAACCCAGACAATA GAAACTGTCCTGCTGTTACCCTGACAAGTCCTGCTAAGACCAAAATACTGCCCCCACGGAAGGGACGGGG ATTAAAATTGGAAGCTATAGTTCAGAAGATCACATCTCCAAATATTAGGAGGAGTGCATCTGCAAACAGT GCTGAGGCTGGGGGAGACACAGTCACACTGGATGATATACTATCTCTAAAGAGTGGTCCTCCTGAAGGTG GCACTGTGGCTACTCAAGAGGCTGAGATGGAAAAGCGAAAGTGTGAGGTGGTATCTGACCTAGTCAGTGT AACTAACCAGGAATCGAATGTTGAGAAGCCTCTTCCAGGGCCTTCTGAAGAGTGGCGTGGCAGTGGGGAT GACAAAGTCAAGACAGAGGCACATGTAGAAACAGCTTCTACTGGAAAGGAACCCTCTGGTACTATGACAT CCACAGCTTCACAGAAGCCTGGTGGTAACCAAGGGAGACCAGATGGTTCCCTGGGTGGGGCAGCACCACT AATCTTTCCTGACTCAAAGAATGTAGCTCCAGTGGGCATATTGGCCCCTGAGGCAAATCCCAAGGCTGAA GAGAAAGAGAACGATACAGTCATGATTTCACCCAAACAAGAAAGTTTCCCCCCTAAAGGGTATTTCCCAT CAGGAAAGAAAAAGGGGAGACCAATCGGTAGTGTCAATAAGCAAAAGAAACAGCAGCAGCAACCACCTCC ACCTCCTCAGCCCCCTCAGATGCCAGAAGGTTCTGCAGATGGGGAGCCAAAGCCAAAAAAGCAGAGGCAA AGGAGGGAGAGAAGGAAGCCTGGGGCCCAGCCAAGGAAGCGGAAAACCAAACAAGCAGTTCCCATTGTAG AACCTCAAGAACCAGAGATCAAGCTAAAGTACGCTACCCAGCCACTAGATAAAACTGATGCCAAGAACAA GTCTTTTTTCCCTTACATCCATGTAGTAAATAAGTGTGAACTTGGAGCTGTTTGTACAATCATCAATGCT GAAGAAGAAGAACAGACCAAATTGGTGAGGAGCCGGAAGGGTCAGAGATCTCTGACCCCTCCCCCCAGCA GCACAGAAAGCAAGGTGCTCCCAGCTTCATCCTTTATGCTGCAGGGGCCTGTGGTAACAGAGTCTTCTGT TATGGGGCACCTGGTTTGCTGTCTCTGTGGCAAGTGGGCCAGTTACCGTAACATGGGTGACCTCTTTGGA CCCTTTTATCCCCAAGATTATGCAGCCACTCTTCCGAAGAATCCACCTCCTAAGAGGTCCTCAGAAATGC AGAGCAAAGTCAAGGTTCGGCACAAAAGCGCTTCTAATGGTTCTAAAACTGACACTGAGGAGGAGGAGGA GCAGCAGCAGCAGAAGGAGCAGAGGAGCCTGGCTGCTCATCCTAGGTTCAAGCGGCGCCACCGCTCAGAA GACTGTGGTGGAGGTCCTCGGTCCCTGTCCAGGGGGCTCCCTTGTAAAAAAGCAGCCACGGAGGGCAGCA GTGAAAAGACTGTTTCAGACACAAAGCCCTCTGTACCTACCACTTCAGAAGGTGGGCCTGAGCTGGAGTT ACAAATCCCTGAACTACCTCTTGACAGCAACGAATTTTGGGTCCATGAGGGTTGTATTCTCTGGGCCAAT GGAATCTACCTGGTCTGTGGCAGGCTCTATGGCCTGCAGGAAGCGCTGGAAATCGCCAGAGAGATGAAAT GCTCCCACTGCCAGGAGGCTGGCGCTACTTTGGGCTGCTACAACAAAGGCTGCTCCTTCCGATACCATTA CCCATGTGCCATTGACGCAGATTGTTTGCTGCATGAGGAGAATTTCTCGGTGAGGTGCCCCAAGCACAAG GTGAGACTGTGGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_013836
Insert Size	5898 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013836.3, NP_038864.3
RefSeq Size	7241 bp
RefSeq ORF	5898 bp
Locus ID	21411
Gene Summary	Transcriptional activator that binds to the regulatory region of MMP3 and thereby controls stromelysin expression. It stimulates the activity of various transcriptional activators such as JUN, SP1, PAX6 and ETS1, suggesting a function as a coactivator.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, compared to variant 1. This results in a distinct C-terminus and shorter protein (isoform b), compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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