Dmbt1 (NM_007769) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CRP; CRP-[a]; CRP-[b]; Crpd; DBMT1; gp300; p80
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225071 representing NM_007769 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGATCTCCACTGTTATCTTTGAAATATGTCTTTTGTGGGGTCAAATTCTGTCTACAGCATCTCAAA CAGCTGTTCCCACAGATGGAACAGATTCTGGTTTGGCCGTGAGGCTGGTGAATGGAGGAGACAGGTGTCA GGGTCGAGTGGAGATCCTTTACCAGGGTTCCTGGGGCACTGTGTGTGACGACAGCTGGGACCTCAATGAT GCCAACGTGGTGTGCAGGCAGCTGGGCTGTGGCTTGGCCGTGTCTGCCCCGGGAAATGCCAGGTTTGGAC AGGGCTCAGGGCCCATTGTCATGGATGACGTGGCCTGTGGAGGCTATGAGGACTATCTGTGGAGATGTTC CCACCGAGGCTGGCTCTCTCATAACTGTGGACACCAGGAAGATGCTGGAGTCATCTGTTCAGATTCTCAA ACAAGCAGTCCCACACCTGGCTGGTGGAATCCAGGAGGGACAAATAACGATGTGTTCTATCCAACTGAAC AAACCACAGCAGAGCAGACAACCATTCCTGATTATACACCAATAGGAACAGATTCTGGTTTGGCCGTGAG GCTGGTGAACGGAGGAGACAGGTGTCAGGGTCGAGTGGAGATCCTTTACCAGGGTTCCTGGGGCACTGTG TGTGATGACAGCTGGGACGTCAGTGATGCCAACGTGGTGTGCAGACAGCTGGGCTGTGGCTGGGCCGTGT CTGCCCCAGGAAATGCCTACTTTGGACAGGGCCAAGGGCCCATTGTCCTGGATGACGTGGCCTGTGGAGG CTATGAGAACTATCTGTGGAGCTGCTCTCACCAAGGCTGGCTCTCTCATAACTGTGGACACCAGGAAGAT GCTGGAGTCATCTGTTCAGCTTCTCAATCAAGCAGTCCCACACCTGGTTGGTGGAATCCAGGAGGGACAA ATAATGATGTGTTCTACCCAACTGAACAAACCACTGCAGGAACAGATTCTGGTTTGGCTGTGAGGCTGGT GAACGGAGGAGACAGGTGTCAGGGTCGAGTGGAGATCCTTTACCAGGGTTCCTGGGGCACTGTATGTGAC GACAGCTGGGACACCAATGATGCCAACGTGGTGTGCAGGCAGCTGGGCTGTGGCTGGGCCGTGTCTGCCC CAGGAAATGCCTACTTTGGACCGGGCTCTGGATCTATTGTTCTGGATGACGTGGCCTGTACAGGACACGA GGACTATCTGTGGAGATGTTCCCACCGAGGCTGGCTCTCTCATAACTGTGGACACCATGAGGATGCTGGA GTCATCTGTTCAGCTTCTCAATCAAGCAGTCCCACACCTGATGTGTTCTACCCAACTGACCAAACCACAG CAGAGCAGACAACCGTTCCTGATTATACACCAATAGGAACAGATTCTGGTTTGGCCGTGAGGCTGGTGAA CGGAGGAGACAGGTGTCAGGGTCGAGTGGAGATCCTTTACCAGGGTTCCTGGGGCACTGTGTGTGACGAC AGCTGGGACCTCAATGATGCCAACGTGGTGTGCAGGCAGCTGGGCTGTGGCTTGGCCGTGTCTGCCCCAG GAAGTGCCAGGTTTGGACAGGGCACAGGGCCCATTGTCATGGATGACGTGGCCTGTGGAGGCTATGAGGA CTATCTGTGGAGATGTTCCCACCGAGGCTGGCTCTCTCATAACTGTGGACACCATGAGGATGCTGGAGTC ATCTGTTCAGCTTCTCAATCAAGCAGTCCCACACCTGATGTGTTCTACCCAACTGACCAAACCACAGCAG AGCAGACAACCGTTCCTGATTATACAACAATAGGAACAGAAAACAGTTTGGCCGTGAGGCTGGAGAATGG AGGAGACAGGTGTCAGGGTCGTGTGGAGATCCTTTACCAGGGTTCCTGGGGCACTGTGTGTGACGACAGC TGGGACCTCAATGATGCCAATGTGGTGTGCAGGCAGCTGGGCTGTGGCTTGGCCGTGTCTGCCCCAGGAA GTGCCAGGTTTGGACAGGGCACAGGGCCCATTGTCATGGATGACGTGGCCTGTGGAGGCTATGAGGACTA TCTTTGGAGATGTTCCCACCGAGGCTGGCTCTCTCATAACTGTGGACACCATGAGGATGCTGGAGTCATC TGTTCAGCTTCTCAATCAAGCAGTCCCACACCTGATGTGTTCTACCCAACTGACCAAACCACAGTAGAGC AGACAACCGTTCCTGATTATACACCAATAGGAACAGAAAACAGTTTGGCCGTGAGGCTGGAGAATGGAGG AGACAGGTGTCAGGGTCGAGTGGAGATCCTTTACCAGGGATCCTGGGGCACTGTGTGTGACGACAGCTGG GACACCAAGGATGCCAACGTGGTGTGCAGACAGCTGGGCTGTGGCTGGGCCGTGTCTGCCCCAGGAAATG CCTACTTTGGACCGGGCTCTGGGTCTATTGTTCTGGATGACGTGGCCTGTACAGGACACGAGGACTATCT GTGGAGCTGCTCTCACCGAGGCTGGCTCTCTCATAACTGTGGACACCATGAGGATGCTGGAGTCATCTGT TCAGATGCCCAAATCCAGAGCACAACCAGGCCAGATCTGTGGCCTACTACTACTACCCCAGAAACTACAA CAGAATTGTTGACTACAACTCCTTATTTTGATTGGTGGACAACTACTTCTGACTACAGCTGTGGAGGTCT CCTGACCCAACCCTCTGGGCAATTTTCCAGCCCATACTACCCTTCAAACTATCCAAACAATGCCAGATGT TCGTGGAAAATTGTGCTCCCCAACATGAACCGCGTGACAGTGGTCTTCACAGATGTGCAGCTTGAAGGAG GCTGCAACTATGACTATATCCTGGTTTATGATGGCCCTGAATACAATTCTTCTCTCATTGCTCGGGTTTG TGATGGTTCCAATGGCTCTTTCACCTCAACCGGAAACTTCATGTCCGTAGTCTTCATCACAGATGGCAGT GTGACAAGGAGAGGGTTCCAGGCTCACTACTACTCTACAGTCAGCACAAACTACAGCTGTGGAGGTCTCC TGACCCAACCCTCTGGACAATTTTCCAGCCCATACTACCCTTCAAACTATCCTAACAATGCCAGATGTTC CTGGGAAATATTGGTCCCCAACATGAACCGTGTGACAGTGGTCTTCACAGATGTGCAGCTTGAAGGAGGC TGCAACTATGACTATATCCTGGTTTATGATGGCCCTCAGTACAATTCGTCTCTCATTGCTCGGGTGTGTG ATGGGTCCAATGGATCTTTCACCTCAACCGGAAACTTCATGTCCGTAGTCTTCATCACAGATGGCAGTGT GACAAGGAGAGGGTTCCAGGCTCACTACTACTCTACAGTCAGCACAACTCCTCCAGTGCCAATCCCAACC ACAGATGACTACAGCTGTGGAGGTCTCCTGACCCTACCCTCTGGACAATTTTCTAGCCCACACTATCCTT CAAACTATCCTAACAATGCCAGATGTTCCTGGGAAATATTGGTCCCCAACATGAACCGTGTGACAGTGGC CTTCACAGATGTGCAGCTTGAAGGAGGCTGCAACTATGACTATATCCTGGTTTATGATGGCCCTGAATAC AATTCTTCTCTCATTGCTCGGGTTTGTGATGGTTCCAATGGCTCTTTCACCTCAACCGGAAACTTCATGT CCGTAGTCTTCATCACAGATGGCAGTGTGACAAGGAGAGGGTTCCAGGCTCACTACTACTCTACAGTCAG CACAAACTACAGCTGTGGAGGTCTCCTGACCCAACCCTCTGGACAATTTTCCAGCCCACACTACCCTTCA AACTATCCTAACAATGTCAGATGTTCCTGGGAAATATTGGTCCCCAGCATGAACCGTGTGACAGTGGCCT TCACAGATGTGCAGCTTGAAGGAGGCTGCAGCTTTGACTATATCCTGGTTTACGATGGCCCTGAATATAA TTCTTCTCTCATTGCTCCGGTGTGTGATGGGTTCAATGGATCTTTCACCTCAACCGGAAACTTCATGTCC GTAGTCTTCATCACGGATGGCAGTGTGACAAGGAGAGGGTTTCAGGCTTACTACTACTCCACAGTCAGCA CAAGTCCTCCATCATTCCCAAACATAACTGGAAATGATTCTTCATTGGCGCTGAGGCTGGTAAATGGATC AAACCGGTGTGAGGGCCGAGTGGAGATCTTGTACAGAGGCTCTTGGGGCACCGTGTGTGATGACAGCTGG GGCATCAGTGACGCCAATGTGGTCTGCAGACAGCTCGGTTGTGGCTCTGCTCTGTCTGCACCAGGAAATG CTTGGTTCGGTCAGGGCTCAGGGCTCATTGTCCTGGATGATGTGTCTTGCTCGGGGTATGAGTCTCACCT GTGGAACTGTCATCATCCTGGCTGGCTTGTCCATAACTGTCGCCATTCTGAGGATGCAGGAGTCATCTGT GCACTCCCTGAGGTGACTTCTCCCAGTCCAGGTTGGTGGACTACAAGTCCTTCCTATGTAAACTATACTT GTGGAGGTTTCCTGACCCAACCCTCTGGGCAATTTTCTAGCCCATTCTACCCTGGGAATTATCCTAATAA TGCCAGATGTCTGTGGAACATCGAGGTCCCAAACAACTACCGTGTGACTGTGGTCTTCAGAGATTTGCAG CTGGAAAGGGGCTGCAGCTATGATTATATAGAGATTTTTGATGGCCCCCACCACAGTTCACCTCTCATCG CCCGGGTTTGTGATGGGTCCCTGGGCTCTTTCACCTCAACATCCAACTTCATGTCAATTCGCTTCATCAC TGATCACAGTATTACTGCGAGAGGGTTCCAGGCTCACTACTACTCCGACTTTGACAATAACACCACCAAT CTCCTTTGTCAGTCAAATCACATGCAAGCCAGCGTGAGCAGGAGCTACCTTCAGTCCATGGGCTATTCTG CCAGGGATCTTGTCATTCCTGGTTGGAACTCCAGCTACCACTGTCAGCCCCAGATAACACAGAGGGAGGT CATATTCACAATTCCCTACACAGGCTGTGGCACAATCAAACAGGCTGACAACGAGACCATCAACTACTCC AACTTCCTCAGAGCGGTTGTTTCAAACGGCATCATCAAAAGGAGAAAGGACCTGAATATCCACGTCAGCT GCAAGATGCTGCAGAACACCTGGGTCAACACCATGTACATCACCAACAACACAGTGGAGATCCAGGAAGT TCAGTATGGCAATTTCGACGTAAACATTTCCTTCTACACATCCTCCTCCTTCTTGTTTCCAGTGACCAGC AGCCCATACTACGTGGATCTGGACCAGAATTTGTACCTTCAGGCCGAAATCCTCCATTCTGACGCCTCCT TGGCTCTGTTTGTGGACACCTGTGTGGCTTCGCCACATCCCAATGACTTCTCATCTTTGACATATGATCT CATCCGGAGTGGATGCGTACGGGATGATACCTACCAATCCTACTCCTCGCCCTCACCACGCGTCTCCCGC TTTAAATTCAGTTCTTTCCACTTCCTGAACCGCTTCCCCTCAGTGTACCTGCAGTGTAAACTGGTAGTAT GTCGAGCGTATGATACCTCCTCACGGTGCTACAGAGGCTGCGTAGTGAGATCTAAGAGGGATGTAGGCTC CTACCAAGAAAAGGTGGATGTTGTCCTGGGACCCATCCAGTTGCAATCCCCCAGCAAAGAAAAGAGGAGC CTCGACTTGGCAGTGGAGGATGTGAAGAAGCCAGCAAGCTCCCAGGCCGTCTACCCTACTGCAGCCATCT TTGGCGGAGTCTTCCTGGCCATGGTCCTGGCTGTGGCAGCCTTCACATTGGGAAGGAGGACACACATTGA CCGTGGCCAACCTCCAAGTACTAAGTTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_007769
Insert Size	5841 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007769.2, NP_031795.2
RefSeq Size	6259 bp
RefSeq ORF	5841 bp
Locus ID	12945
UniProt ID	Q60997
Gene Summary	May play roles in mucosal defense system and cellular immune defense. May play a role in liver regeneration. May be an important factor in fate decision and differentiation of transit-amplifying ductular (oval) cells within the hepatic lineage. May function as a binding protein in saliva for the regulation of taste sensation. May play a role as an opsonin receptor for SFTPD and SPAR in macrophage tissues throughout the body, including epithelial cells lining the gastrointestinal tract (By similarity). Required for terminal differentiation of columnar epithelial cells during early embryogenesis. Displays a broad calcium-dependent binding spectrum against both Gram-positive and Gram-negative bacteria, suggesting a role in defense against bacterial pathogens. Binds to a range of poly-sulfated and poly-phosphorylated ligands which may explain its broad bacterial-binding specificity. Inhibits cytoinvasion of S.enterica. Associates with the actin cytoskeleton and is involved in its remodeling during regulated exocytosis. Interacts with pancreatic zymogens in a pH-dependent manner and may act as a Golgi cargo receptor in the regulated secretory pathway of the pancreatic acinar cell.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript was mostly derived from the reference genome assembly. The genomic coordinates were determined from alignments.

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