Atg2a (NM_194348) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1810013C15Rik; A830054M12; BC023754; mKIAA0404
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225033 representing NM_194348 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACGATGGCTGTGGCCATGGTCGAACTGTGTGAAAGAGCGTGTCTGCCGCTACTTGCTACAGCACT ACTTGGGTCACTTCTTTCAGGAGCACCTCAGCCTGGACCAGCTCAGCCTGGATCTGTACAAGGGCAGCGT TGCCCTGCGGGATATTCACCTGGAAACCTGGTCTGTGAACGAGTTTTTGAGGTCAATGGAGTCACCACTG GAACTGGTGGAAGGCTTTGTGAGCTCCATCGAGGTGGCTGTCCCCTGGGCTGCACTTCTCACTGACCACT GCACTGTGTGTGTGTCAGGCCTTCAGCTTACCCTGCAGCCCCGCCAGGGTTCAGGACCAGGGGCTGCGGA CTCTCAGAGTTGGGCCTCATGCATGACCACAAGCTTGCAGCTGGCTCAAGAGTGCCTTCGGGAAGGGTTG CCAGAACCCTCAGAACCACCACAGCCCCTGGAGGGGCTGGAAATGTTTGCACAGACCATTGAGACTGTGC TCCGGAGGATCAAGGTGACCTTCCTGAACACTGTTGTGAGGGTGGAGCATTCACTGGGTGATGAGGATCG AAGTGTGGCTGTGGAGGTCCGAGTGCAGAGACTGGAGTACTGTGACGAAGCAGTGCGGGACCCCAGCCAG GCCCCACCTGTGGACGTGCACCAGCCGCCTGCCTTCCTGCACAAGCTGCTGCAGCTGAGTGGGGTCTGCC TATACTTTGAGGAGCTGCCCTCCCAGGCAGACCCACCACAGCCACCTCTGCAAATCGGCAGCTGCACGGG GTACGTGGAGCTCATGGTGAGGCTGAAGCAGAACGAGGCCTTTCCAGGCCCCAAGTTGGAGGTATCAGGA CAACTGGGATCCCTGCACCTGCTTTTGACCCCGCGTCAGCTCCAGCAGCTCCAGAGGCTGCTCAGTGCCG TCAACCTTGCAGACCCTGCAGGCCTGGCCGACAAGCTGAACAAGAGCCGTCCACTGGGTGCTGAGGACTT GTGGCTCATTGAGCAAGATCTGAACCAGCAGCTGCAGGCGGGTGCTGTGGCTGAGTCCCTCAGCCTGTAC CCCATCACCAACCCCCTCAATTTGGACAGCACGGACCTCTTCTTCTCCATGGCCGGCCTCACAAGCAGTG TGACATCGGCTGTCTCTGAGCTCTCCGTGTACAGCGTAGACCTGGGCTCCTCTGTTCACAGCAACATGGC CTTCCACCGACCCTCAACCCCACCCCACTCGGGTGGCAAGATGGCCCCAACTCCCCTTCTGGACACCACA CGCCCTGACTCACTGGTGAAGATGACCTTGGGCGGTGTGAGCTTGACCTTGCTGCAGACTGCTTCCCCGT CTTCAGGACCATCTGACCTCCCCACCCACTTTTTTGCTGAGTTCGATGCTGCCAAAGATGGGCCATTCGG CTCCCGGGACTTCTCCCATCTCAGGCCACGCTTCCAGAGGGCCTGCCCTTGTAGCCACGTCCGGTTAACT GGCACAGCCGTGCAGCTGTCCTGGGAGCTGCGCACGGGCAGCCACAGCCGGAGGACAAGCAGCACCGAAG TGCACTTTGGACAGCTGGAGGTGCTGGAGTGCCTATGGCCCAGGGCTGCCACAGAGCCAGAGTACACGGA GATCCTGAGCTTCCCCAGTCACTCTGGCTCCGAAGCCTCAGCCCGACCCTGCGCCCATCTGCGCCACACA CAGACCATTCGCCGGGTGCTCAAGAGCCGGTCCCGGCGCTCCACTGCCTGCCATTGTCACTCAGAACTGT CCCTGGACCTGGCCGACTTCCAATCTGATGTGGAGCTGGGGTCCCTGGACCGCCTTGCTGCCTTGTTCCG TCAAGTCACCACACCCTCTGAGCCACCTGCTGGCCTACTAACAGAGCCCCCACAGGCCACTGAGCTGCAG ACAGTGTTTCGTCTCTCAGCACCCCGGGCCACCCTGAGGCTACGCTTCCCCATCCCAGACCTACGGCCTG ACCGGGACCCCTGGGCTGGCCAGGCTGTGCGGGCTGAGCAGCTGCGACTGGAGCTGAGTGAGCCCCAGTT CCGCTCAGAGCTCAACAGTGGGCCTGGCCCCCCAGCTCCTACCCGCCTGGAACTTACTTGCTCAGACCTG CAAGGAATCTACGAAGATGGAGAGAAGCCACCAGTCCCCTGCCTGCGGGTCTCCAAAGCTCTGAATCCCA GGAGCACTGAGGCCAAGTACTTTCTACCTCAGGTAGTGGTGACCCTGAACCCCCAGTCCAGTGGCACACA GTGGGAGACAGCCTATGAGAAAGGCCGGGATCTAGAGCTGTCCACCGAGAGTCCGTGTGAGCTACAGCAA CCTGAGCCCTCGCCCTTCTCCTCCAAGCGGACCATGTACGAGACTGAGGAAATGGTGATCCCTGGAGACC CTGAAGAGATGAGGACATTCCAGAGCCGGACACTGGCACTGTCCCGCTGTACTCTGGATGTGATCATGCC CAGTGCCCACATCTTCCTGCCCAGTAAGGAGGTCTACGAAAGTATCTACAACAGGATCAATAACGACCTG CTCATGTGGGAGCCTGCAGACCTGCTTCCCACATCCAGCGCTGCTGCTCGGCCGCCTGGCTCTTCGGGGT TCAAGATGTGCAAGTCAGCCTTCAAGCTAGACTCGGATTCAGATGAAGAAGACGCCCAGTTCTTCTCGAT GGCATCAGGTGTCCCCCAGACACCTGCCCCAGAACCTTCCCGTCGTCAGTCCCAGAGTACCTTCTCCACA CTGGTGACAGTGCTGAAGGGTCGGATCACTGCGCTCTGTGAGGCTAAGGATGAGACAGGGAAGCGGCTGG ATGTCACTCACGGGGAGCTGGTACTAGATGTGGAGCAAGGCACCATCTTCAGCGTAGCCCAGTACCGTGG CCAACCAGGGCTTGGCTACTTCTGCCTGGAAGCTGAAAAGGCCAAACTCTACCATCGAGCGGCCATTGAA GACTACCTGCTACCCACTCATTTGGAAGTGCCCAGCTTTGCTCCCCCCGCTCAGCTGGCTCCAACCATCT ACCCATCAGAGGAAGGGGTGACTGAGCGAGGAACCTTGGGCCGCAAGGGCCAGGGTCCCCCCATGCTGTC TGCAGCTGTCCGTATCCACCTGGACCCTCACAAGAATGTCAAGGAATTTTTGGTGACAGTACGGCTGCAC AAAGCCACTCTACGCCACTACATGGCCCCACCTGAACAGAGCTGGCACTCCCAGCTCCTGGACTTCTTGG ATGTGCTGGATGACCCTGTGCTGGGATACCTGCCCCCAACCGTCATCACTGTCCTGCACACACATCTCTT CTCCTGCGCTGTGGACTACAGGCCCCTCTACCTCCCTGTGCGTGTCCTGGTCACTGCTGAGACCTTCACC CTCTCCAGCAACATTGTCATGGACACGTCCACCTTCCTGCTCAGGTTCATCCTTGATGACTCCGCTCTGT ACCTGTCTGACAAGTGTGAGGTGGAGAGCCTGGATCTGCGGCGAGATTATGTCTGTGTCCTGGATATTGA CCTCCTGGAACTTGTCATCAAAACCTGGAAGGGCAGCACTGAGGGCAGACTGAGCCAGCCTCTGTTTGAG CTGCGCTGCTCCAACAACGTGGTGCACGTGCATAGCTGTGCTGACTCCTGCGCCCTGCTGGTCAACCTGC TCCAGTACCTAACCAGCTCGGGTGACCTGCACCCCCCACCCCGGCCCCCCAGCCCTACAGAGATCGCTGG CCAGAAGCTTTCGGAGAGCCCTGCCTCCCTGCCCTCATGCCTACCTGTGGAGACAGCCCTCATCAACCAA CGAGACCTGACTGACGCCCTTCTGGATACTGAACGGCGCGGCCTGCAGGAGCTGGCCCAGTCATCGGGTG GCCCACTTCCTCAGGCCTCTCCCGTCTCCGTCTATCTGTTCCCAGGTGAACGGAGTGGGGCCCAGGCCCC TTTGCCTCCTCCTGGGGCCTCTTCTCATACCTTGGGTTCTAAGGCAAAGGAACATGAAAACGAAGAGGAA GGGGACGGAGACACCCTGGACAGTGATGAGTTCTGCATCCTTGACGCCCCTGGCCTAGGCATTGCGCCTC GAGACGGGGAGCCCATCGTGACACAGCTGCATCCAGGCCCCATCATCGTGCACGACGGTCACTTCTCCCA GCCCCTGGGAAGCACTGACCTGCTGCGCGCTCCTGCCCACTTCCCAGTGCCCAGCAGCCGTGTGGTACTA CGGGAGGTCTCCTTCATCTGGCACCTCTATGGGGGTAGAGACTTTGGCCTTCACCCCACTTACAGAGCAA GAGTAGGCCTCACAGGCCCCAGGGTCTCACCTTCCCGCTCCTCTGGTCCCAACCGTCCCCAGAACTCCTG GCGTACCCAGGGTGGCATTGGACGGCAACACCAGGTCCTCATGGAGATCCAGCTCAGCAAAGTGAGCTTC CAGCATGAAGTGTACCCAGAAGAGTCGGCCATAGCTGGGGGCCTGGGCCAGGAGCTGGACGAGCGACCAC TGTCGCGCCAGGTGCTCATTGTTCAAGAGCTAGAGATCCGAGACCGGCTGGCCACCTCCAAGATCAACAA GTTTCTTCATTTGCACACGAGTGAGCGGCTGCCACGGCGCACACACTCCAACATGCTTACCATCAAGGCA CTGCATGTGGCTCCCACCAGCAGCGTCGGAGGGCCGGAGTGCTGTCTTCGAGTCTCCATGATGCCTCTGA GGCTCAATGTGGACCAGGATGCCCTCTTCTTCCTCAAGGACTTTTTCACCAGCCTAGCAGCCAGTATCAA CCCCATGGTCCCAGGGGACACTTCTGAGGCTCCCCGAGAGACCCACAGCCGGCCCGGCAGCCCCCAGGAA GGGCAGTCTGAGGACACAGAGACAGCCAGCAACCCACCGGAAGCTCCAGGCAGTAGCCACAGCTCCTCTG ACCAGCAGCCTATCTACTTCAGGGAGTTCCGCTTCACATCTGAGGTGCCCATCTGCCTGGATTACCATGG CAAGCATGTCACTGTGGACCAGGTGGGCACTTTCATGGGACTCCTAATCGGCCTGGCCCAGCTCAACTGC TCAGAGCTGAAGCTCAAGAGGCTCTGCTGCCGGCATGGGCTCCTGGGTGTGGACAAGGTGCTGTGCTATG CCCTCAATGAATGGCTGCAGGACATCCGTAAGAACCAGCTGCCCGGCCTGCTGGGAGGCGTGGGCCCCAT GCACTCGGTTGTGCAGCTCTTCCAAGGGTTCCGGGACCTGCTGTGGCTACCCATCGAGCAGTACAGGAAA GATGGGCGCCTCATTCGGGGGCTGCAGCGTGGGGCTGCCTCCTTTGGGTCATCCACAGCCTCTGCTGCTC TGGAACTGAGCAACAGGCTGGTCCAGGCCATCCAGGCTACAGCTGAGACAGTGTATGACATCCTGTCCCC GGCTTCTCCTGTCTCCCGATCCCTACAAGACAAGCGCTCTTCGCGGAAGCTACGCAGAGGTCAACAACCT GCTGACCTCCGGGAAGGCATGGCTAAGGCTTATGATGCTGTTCGAGAGGGTATCCTGGATACAGCTCAAA CCATCTGTGATGTGGCATCCAGGGGCCATGAGCAGAAGGGACTGACAGGTGCCGTGGGGGGTGTGATCCG ACAGCTCCCCCCAACCGTGGTAAAACCAATAATCGTAGCAACAGAGGCAACATCCAACGTGCTAGGGGGC ATGCGTAACCAGATCCTTCCAGATGCGCACAAGGACCATGCTCTCAAGTGGCGATTGGAGGAGGCCCAGG ACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_194348
Insert Size	5745 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_194348.3, NP_919329.2
RefSeq Size	6340 bp
RefSeq ORF	5745 bp
Locus ID	329015
UniProt ID	Q6P4T0
Gene Summary	Involved in autophagosome assembly, regulating the size of nascent autophagosomes. Also regulates lipid droplets morphology and distribution within the cell.[UniProtKB/Swiss-Prot Function]

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