Dennd4a (NM_001162917) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI115600; AI851943; F730015K02Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224995 representing NM_001162917 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTGAAGACAAGGGACCTCGTGTTGCTGACTACTTTGTTGTAGCAGGCTTAACTGACATTTCAAAGC CTCTTGAAGAGGAAATTCACTTCAATGATGCCTGTCACAAAGTGGCAAAACCAAAAGAACCCATCACAGA TGTTTCTGTTATCATCAAATCCCTTGGGGAGGAGGTGCCACGGGATTATGTGTGTATTGATGTTACCCCA ACTGGATTATCAGCAGACCTTAATAATGGAAGTCTTGTGGGACCCCAGATTTACCTTTGCTATAAAAGAG GAAGAGATAAACCTCCACTTACAGATCTGGGGGTACTATATGACTGGAAAGAGAGACTGAAGCAGGGCTG TGAGATTATTCAGAGTACTCCCTATGGACGACCTGCCAATATTAGCGGCAGTACCTCATCACAAAGAATC TACATCACTTACCGAAGAGCCTCTGAAAACACGACTCAGAATACGCTGGCTGTTACAGACATATGCATTA TTATACCCAGTAAAGGAGAGAGCCCACCACACACATTCTGCAAGGTCGACAAGAATCTCAGCAATAGTAT GTGGGGATCTGCGGTATACTTATGCTATAAAAAGTCAGTGGCAAAGACTAACACTATATCTTACAAAGCA GGTCTAATTTGCAGATACCCTCAAGAAGATTACGAGTCGTTCTCACTGCCAGAATCTGTTCCCCTCTTTT GTTTACCAATGGGGGCAACTATTGAATGCTGGCCACCAGATAGCAAATACCCTCTTCCTGTGTTCTCTAC CTTTGTGTTAACCGGAGCCTCAGCTGAGAAGGTCTATGGTGCTGCTATTCAGTTTTATGAACCATACCCT GAGGAGAACCTTACAGAAAAGCAGAGACTTCTGTTGGGTTTAACGTCTCTGGTAGATGGGAAGTCTGATA GTACTAGAACAATTCACACTAACAAATGCATCTGTCTTCTTTCTCATTGGCCTTTTTTTGATGCATTCAG AAAATTTCTGACTTTTCTGTATCGTTATTCCATCTCTGGACCTCATGCCCTTCCAATTGAAAAGCATATT TCTCATTTTATGCATAAAGTTCCCTTTCCATCTCCTCAGAGGCCACGGATTTTAGTTCAGTTATCCCCAC ATGATAATTTGATTCTCAGTCAGCCTGTTTCTTCCCCTCTTCCATTAAGTGGTGGAAAGTTTTCAACACT ACTCCAGAATCTTGGCCCTGAGAATGCTGTGACACTGCTGGTGTTTGCTGTAACAGAACATAAAATCCTC ATCCATTCCCTACGGCCCTCTGTGCTCACCAGTGTGACAGAAGCATTAGTGTCTATGATTTTCCCTTTCC ACTGGCCCTGCCCGTATGTCCCTCTGTGCCCACTGGCCCTCGCAGACGTCCTCAGTGCACCATGTCCATT CATAGTAGGCATCGATTCCAGATACTTTGATCTTTATGACCCTCCACCAGATGTTAGTTGTGTCGACTTA GACACCAACACCATTTCTCAAACTGGGGACAAAAAGAATGTAGCCTGGAAGATTCTCCCAAAGAAGCCAT GTAAAAATCTGATGAATACACTGAACAATTTACACCAGCAGTTGGCAAAATTGCAGCAGAGACCAAGAGA TGATGGACTAATGGACTTAGCAATGAATGATTATGATTTTAATTCCGGGAAGAGGTTGCACATGATAGAT CTGGAAATCCAGGAGGCATTCTTGTTTTTCATGGCCTCTATTTTAAAAGGTTATAGATCATACTTACGGC CAATAACACAAGCCCCGTCTGAGACAGCGACAGACGCAACCTCTCTTTTTGCCCTTCAAGCTTTCTTAAG AAGCCGGGACCGGTCACATCAAAAATTCTATAACATGATGACCAAAACACAAATGTTTATTCGCTTCATT GAGGAATGTTCTTTTGTCAGCGATAAAGATGCAAGCCTGGCCTTTTTTGATGACTGTGTAGATAAAGTGG ATACAGACAAGAGTGGTGAAGTTCGACTTATAGAACTGGATGCATCTTTCAAAAGTGAACATACCGTTTT TGTGACACCACCAGAGATCCCCCATCTACCCAATGGTGAAGAGCCACCATTACAGTACAGCTATAATGGA TTTCCAGTTCTCAGGAACAATTTATTTGAGAGGCCTGAAGGATTTCTACAAGCACGAAAGAACAAACTAC CTTCAAAACCAAGCAGTCCTGGTAGTCCTTCTCCCATGTTCAGAAGAACAAAACAGGAAATCAAGTCAGC CCATAAGATCGCAAAGAGGTATTCTTCTATCCCTCAGATGTGGTCTCGGTGTCTGCTGCGTCACTGTTAT GGATTGTGGTTTATTTGTCTCCCAGCTTACGTGAAGGTTTGTCACTCCAAAGTCAGGGCTCTGAAAACTG CATACGATGTGCTTAAAAAAATGCAGCTGAAGAAGATGGATCCACCTGATGAGGTGTGCTACCGCATTCT CATGCAGCTCTGTGGACAGTACGACCAGCCTGTACTTGCGGTTCGAGTGCTTTTTGAAATGCAGAAAGCT GGTATTGACCCCAATGCAATCACTTATGGATATTATAATAAGGCTGTTTTAGAAAGTACGTGGCCTTCAA GAAGTCGTAGTGGCTATTTCCTCTGGACAAAAGTAAGAAATGTTGTTTTAGGAGTTGCACAGTTCAAAAG AGCTTTGAAGAAACATCCACACTTACCACAGACAGCGCTCTCAGGTGGCCGGTCTGATCTTGGATATAAT TCATTATCTAAGGACAATGTTAGAAGAGAGGGTCCATCTACTGAAGACATTCAAGGGGAAAAGGAGAAAA GAGGGAGTGATTCTAGTTCCTTGTCAGAAAATGAGAGTACAAAAGGAAGTGCGGAGTGCCTCCCTACTCT CAGTTACCAGAGGGCTTCCACTATAGTTCGCCTCAACGGTGCACATAACGACAGTGCTGGTAAAACATCC GGAGAATGTGAGGAAAGCACACCTGAGCTGCTCTTAATGCCATCTCTTGAGGACACAAATGAAGCACAAA CCACGCCAAGCAGGTGCTTTAGGAAAAGGCATAAAAGTGATGATGGATCTCACTTGCAGCAGCAAATGCC TTGGGGAAGCAGGAACCGTAATCTCAGTGGTGGGGTACTGATGGGATTCATGCTGAACAGAACTAACCAG GAAACAAGCCCTGGAGAGATGGTTGAAAAATTAGGAGCTGATGCAAAAATTCTTTCAAATGTTATCTCAA AAAGCACAAGGCCAAATAGTCTTGATATTGGAAAACCACCCGCAAGATCAAAGAGAGACAGCCTAGAGAA GGAGTCTAGCGACGATGACACACCTTTCCATGGTTCTAACTGCCTGGATAAAGTCGAGTCTCCTGTTATC TTTGACTTAGAAGATTTAGACACTGAAACAGATGGATCAAAAGTGGGCGGTGTCGCTGCACAAAATCCCA AGAGGCTTCAACGTAGGAACAGCAGCTTTTCAGTAAAACCCTCTGAAAAAACAGATGTTGTAACAGGCTT TGATCCTCTCTCGCTTTTGGTTGCTGAGACTGAGCAGCAGCAGAAAGTGGAAGAGGAAGAGGATGAAGAT GATAACAAGAGCGTCTCAACACCATCTGCCAGGCGCAATTTAGCTGAAGAAATCGAGATGTACATGAATA ACATGAGCAGTCCTTTGACAAGCCGCACACCCAGCATTGATTTACAACGAGCATGTGATGACAAGTTAAC CAATAAGAAAAGCCCAACATTGGTCAAGGCCTGCAGAAGATCAAGCCTGCCTCCTAATTCTCCTAGGCCA GTGAGACTTACGAAATCCAAAAGTTACACCAAAAGTGAGGAGAGACCCAGGGACAGACTGTGGTCCTCCC CAGCCTTCTCGCCCACGTGTCCATTTCGGGAAGGATCTCAAGAAACACTAGCCCATTCATCACCTTCATT TAACTTAGACACACTGCTGGTGCCTAAACTTGATGTCTTAAGGCACAGTGTGTTCACTGCTGGGAAAGGA GTTGCGGAGAAAGCAAGCAAATGGTACTCAAGGTTCACCATGTACACTACGTCATCAAAGGATCAAAGTT CTGATCGTACCAGTCTTTCTTCAGTGGGAGCCCAGGATTCTGAATCTACCTCTTTGACAGATGAAGATGT CTGCCACGAGTTGGAAGGAGCCACTTCCTCCCAAGAAAGCAGTGCGGCTTCGGGAACCAAGGGGATTGAC GTCAGCAGGGCCAGCCTGGGAAGCTCTGCCTCCTTAGAAGGATCCCTGTCAAAGTTTGCCTTGCCTGGAA AATCAGAGACTGCATCTTCTCTCAACACCAGTAACGCCAATATCTTCCAGAACTACGCAATGGAGGTTCT CATCTCAAGTTGTTCTCGGTGTAGGACCTGCGACTGTCTTGTCCATGATGAGGAAATCATGGCTGGCTGG ACAGCAGATGATTCAAATCTCAATACTACATGTCCATTTTGTGGCAATCTCTTCCTACCCTTTCTGAATG TTGAAATCAGAGACTTAAGACGACCTGGGAGATATTTCTTGAAGTCCAGCTCATCAACAGAAACTATGCA CTTTGCAAGTCGGACAAGGCAGTCTTGCATCTCAGCATCGGCCTCTGGTCTTGACACTTCTTCTCTCTCG GTTCAAGGGAACTTTGATCTAAATAACAAATCTAAACTGCAGGAAAATCCATGTGCCCGAAGTATTCAGA TCCCTGCTCACAGATCAAAAACTGTTGTGTCAAAATGCCCGCTATTCCCAATGGCCAGAAGCATCAGCAC ATGTGGCCCCTTGGATAAGGACGATCCCGGAGGACAGAAGCTCATTCCTACAGGCAGCCTGCCAGCGACT TTACAAGGACATACAGATTCTTTAGGGTTAGAATGGCACCTGCCGAGTCCAGACCCTGTCACTGTCCCAT ACCTTAGTCCCTTAGTGGTGTGGAAGGAACTTGAGAGCTTATTGGAGAATGAAGGTGACCATGCAATAAC AGTGGCAGACTTTGTGGACCATCACCCAATTGTCTTTTGGAACCTGGTGTGGTATTTTAGACGCCTCGAC TTGCCCAGTAACCTGCCTGGACTGATTCTTTCTTCTGAGCATTGTAACAAGTATTCAAAGATTCCCCGCC ACTGTATGTCTGAAGATAGCAAGTATGTTTTAATACAGATGTTATGGGACAACATGAAATTACATCAGGA TCCAGGGCAACCCTTGTACATCCTCTGGAATGCCCACAGTCAGAACCGCACTCTTCTGTTTGAGACTCAA AAGTACCCAATGGTCCATTTGTTACAAAAAGGTGATGACTCCTTTAACCAGGAACTGCTGAAAAGCATGG TGAAGAGCATTAAAATGAACGATGTCTATGGGCCCATGAGTCAGATTTTAGAGACTCTGAATAAGTGTCC GCATTTTAAAAGACAGCGGAGTTTATATAGAGAAATATTATTTCTCTCACTTGTGGCACTGGGAAGAGAA AACATTGATATAGATGCATTTGACAAAGAATACAAGATGGCTTATGATCGTCTCACGCCGAGTCAAGTCA AGAGTACACATAACTGTGACAGGCCACCAAGTACTGGAGTGATGGAATGCCGAAAAACCTTCGGAGAACC TTATCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001162917
Insert Size	5610 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001162917.1, NP_001156389.1
RefSeq Size	8655 bp
RefSeq ORF	5610 bp
Locus ID	102442

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