Myo5b (NM_201600) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI661750; mKIAA1119
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224947 representing NM_201600 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTACAGCGAGCTCTACACCCGGTACACAAGGGTCTGGATCCCTGACCCAGATGAGGTGTGGCGCT CGGCTGAATTAACCAAGGACTACAAAGAAGGGGATAAGAGCCTGCAGCTCAGACTAGAAGATGACACAAT TCTAGAATACCCAGTTGATGTCCAAAATAACCAGGTGCCATTCTTGCGGAATCCAGATATCTTAGTAGGA GAAAACGACCTCACTGCCCTCAGCCACCTCCACGAGCCCGCAGTTCTGCATAACTTAAAGGTCCGTTTCC TGGAATCCAACCACATCTACACTTACTGTGGAATTGTGCTTGTTGCCATCAACCCCTACGAGCAGCTGCC AATCTACGGACAGGATGTCATCTATGCCTACAGTGGCCAAAACATGGGTGACATGGACCCCCACATCTTT GCTGTGGCAGAAGAAGCCTACAAGCAGATGGCCAGAGATGAAAAGAACCAATCCATCATAGTCAGCGGAG AGTCTGGAGCAGGCAAGACGGTGTCGGCCAAGTACGCCATGCGCTATTTTGCCACCGTTGGTGGCTCAGC CAGTGATACCAACATTGAAGAGAAGGTCCTAGCATCCAGCCCCATCATGGAGGCCATTGGGAACGCCAAG ACTACTCGCAACGACAACAGCAGCCGATTTGGGAAATTCATTGAGATTGGCTTTGATAAAAAGTACCACA TCATTGGGGCCAACATGAGGACCTACTTGCTGGAGAAGTCCAGGGTGGTCTTTCAGGCGGATGACGAGAG GAACTACCACATCTTTTACCAGCTCTGTGCTGCTGCCAGCCTTCCTGAATTTAAAGAGCTCGCCCTAACG TGCGCAGAGGACTTTTTCTATACAGCCCATGGAGGAAACACGACCATCGAGGGTGTGAATGATGCAGACG ACTTTGAGAAGACTAGACAAGCCCTCACACTCCTGGGAGTGCGGGATTCCCATCAAATAAGCATCTTTAA GATAATTGCTTCTATCTTGCACCTGGGAAGTGTGGAGATTCAGTCTGAGCGGGACGGGGATTCCTGCAGC ATTTCACCCCAGGATGAACACTTGAGCAACTTCTGCAGCCTGCTAGGAATAGAACACAGCCAGATGGAGC ACTGGCTGTGTCATCGGAAGCTGGTCACCACCTCAGAGACCTACGTCAAGACCATGTCCCTGCAGCAAGT GGTCAATGCACGTGATGCCTTAGCCAAGCACATCTATGCTCAGCTGTTCTCCTGGATTGTGGAGCACATC AACAAAGCCCTGCACACCTCCCTTAAGCAGCACTCCTTCATCGGGGTCCTGGATATCTATGGGTTTGAGA CCTTCGAGATCAATAGCTTTGAACAGTTTTGTATCAACTACGCCAATGAAAAGCTCCAGCAACAGTTCAA TTCGCATGTGTTCAAGCTGGAGCAAGAAGAATATATGAAGGAGCAGATCCCGTGGACTTTGATTGACTTC TACGATAACCAACCGTGTATAGACCTCATAGAGGCAAAGCTGGGCATCCTGGACCTGTTGGATGAGGAGT GTAAGGTCCCCAAAGGAACTGATCAGAACTGGGCCCAGAAACTCTACGAACGACACTCCAACAGCCAGCA CTTCCAGAAACCACGCATGTCCAACACGGCCTTCATTGTCAACCACTTTGCAGATAAGGTGGAGTACCTT TCAGATGGTTTTCTAGAGAAAAACAGGGATACAGTATATGAAGAACAGATCAACATCCTGAAGGCCAGCA AGTTCCCATTAGTGGCTGATTTGTTCCACGATGACAAGGACTCTGCTCCAGCCACCAACACAGCTAAGAA TCGATCATCTTCAAAGATCAACGTTCGCTCTTCCAGACCCCTCATCAAAGTCCCTAACAAGGAGCACAAG AAATCCGTGGGCTACCAGTTTCGTACTTCCCTAAACCTGCTTATGGAGACCCTGAATGCCACAACGCCAC ACTATGTCCGGTGCATCAAGCCCAACGATGAAAAGCTTCCCTTCCACTTTGACCCCAAGAGAGCTGTACA GCAACTCCGAGCCTGCGGTGTGCTGGAGACCATTCGGATCAGCGCGGCAGGCTACCCATCCAGGTGGACC TACCATGACTTCTTCAACCGGTATCGGGTGTTGATGAAGAAGAGAGAGCTCACCAACACCGACAAGAAGA ATATCTGCAAGTCTGTCCTGGAGAGTCTCATCAAGGACCCAGACAAGTTCCAGTTTGGCCGCACCAAGAT CTTCTTCCGAGCAGGCCAGGTGGCCTACCTGGAGAAGCTTCGGGCAGACAAGTTCCGGGAAGCTACTATC ATGATCCAGAAGTCGGTCAGGGGATGGCTGCAGAGAGTGAAGTACCGTAGGCTGAGGGCAGCTACCTTAT CCCTGCAGAGGTTCTGCAGGGGATACCTAGCCCGCAGGCTGGCCGAACACTTGAGGAGAACCCGGGCCGC CATCGTGTTCCAGAAGCAGTACCGCATGCTGAAGGCCCGCCGAGCCTACCGCAGGGTCTGCAGGGCTACC GTCATCATCCAGTCCTTCACACGGGCCATGTTTGTGCGCAGGAACTACCGCCAGGTCCTCATGGAGCACA AAGCCACCATCATCCAGAAGTACGCCCGAGGCTGGATGGCACGGAAGCGTTTTCTGCGGGAGCGGGATGC AGCCATTGTCATCCAGTGTGCCTTCCGGAGACTCAAGGCCAGGCAGGAGCTGAAGGCCCTCAAGATTGAG GCCCGCTCGGCAGAGCATCTGAAACGCCTCAACGTGGGCATGGAGAACAAAGTCGTCCAGCTACAGCGGA AGATTGATGACCAGAACAAAGAGTTCAAGACTCTGTCAGAGCAGCTGTCTGCAGTCACCTCCAGTCATGC CGTGGAGGTGGAGAAGCTGAAGAAGGAGCTGGCACATTACCAGCAGAACCAGGAGGCTGATACCAGCCTT CAGCTGCAGGAGGAGGTTCAGAGCCTGCGCACTGAACTACAGAAGGCTCATTCGGAGCGCAGGGTCCTGG AGGATGCTCACAACAAGGAGAATGGCGAGCTGAGAAAGCGAGTTGCAGACCTGGAACATGAAAATGCCCT CTTGAAGGATGAAAAAGAATACCTTAACAACCAAATCCTGTGCCAGTCAAAAGCTGAATCTTCGCAGAGC TCTGTGGAAGAGAACCTCCTGATGAAGAAGGAGCTGGAGGAGGAACGGTCCAGGTACCAGAACCTCGTGA AGGAGTACTCCCAGCTGGAGCAGAGATATGAGAACCTTCGGGACGAGCAAACTCCGGGCCACAGGAAGAA CCCATCGAACCAAAGCAGCTTAGAATCTGACTCCAATTACCCCTCCATTTCCACTTCCGAAATCGGAGAC ACTGAGGATGCCCTTCAGCAGGTGGAGGAGATTGGCATAGAGAAGGCAGCCATGGACATGACCGTCTTCC TGAAGCTGCAGAAGAGAGTGCGCGAACTTGAGCAGGAGAGGAAGAAGCTGCAGGCACAGCTAGAAAAGGG ACAGCAGGACAGCAAGAAAGGGCAGGTAGAACAACAGAACAATGGCTTAGATGTGGACCAGGACGCAGAT ATAGCCTACAATAGTCTGAAGAGACAGGAGCTTGAGTCAGAGAACAAGAAGCTGAAGAATGACCTGAATG AGCTGAGGAAGGCTGTCGCTGACCAAGCCATGCAGGATAACTCCACCCACAGCTCCCCAGACAGCTACAG CCTCCTCCTGAACCAGCTCAAGCTGGCCAATGAGGAGCTCGAGGTCCGCAAAGAGGAGGTGCTGATCCTC AGGACCCAGATCATGAATGCCGACCAGCGCCGCCTGTCTGGCAAGAACATGGAGCCGAACATCAATGCCA GAACAAGTTGGCCCAACAGTGAGAAGCACGTGGACCAGGAAGACGCCATTGAGGCCTATCACGGGGTCTG CCAGACAAACAGGTTGCTGGAGGCCCAGCTGCAGGCCCAGAGCCTGGAGCATGAGGAGGAGGTGGAACAT CTCAAGGCCCAGGTGGAAGCCCTGAAAGAGGAGATGGACAAACAGCAGCAGACCTTCTGCCAGACCCTGC TGCTCTCCCCAGAGGCCCAGGTAGAATTTGGTGTCCAGCAGGAGATATCCCGGCTGACCAATGAGAACCT GGATTTTAAGGAATTGGTGGAAAAGCTGGAGAAGAATGAGAGGAAGCTGAAGAAGCAGCTGAAGATTTAC ATGAAGAAGGTCCAGGACTTAGAAGCTGCTCAGGCGTTGGCACAGAGTGACAGGAGACACCATGAACTCA CAAGACAGGTCACAGTCCAACGAAAAGAGAAGGACTTCCAAGGCATGCTGGAGTACCACAAAGAGGACGA AGCCCTCCTCATCCGGAACCTGGTGACAGACCTGAAGCCTCAGATGCTGTCGGGCACCGTGCCCTGTCTG CCTGCATACATACTCTATATGTGCATCAGGCACGCGGATTACACCAACGATGACCTCAAGGTGCACTCGT TGCTGAGCTCCACCATCAACGGCATTAAGAAAGTCCTCAAGAAGCACAATGATGACTTTGAGATGACGTC ATTCTGGCTATCCAACACCTGCCGCTTCCTTCACTGTCTGAAGCAATACAGTGGTGATGAGGGTTTCATG ACACAGAACACTGCGAAGCAGAATGAGCACTGTCTCAAGAACTTTGACCTCACTGAATACCGCCAGGTGC TAAGCGACCTTTCCATTCAGATCTATCAGCAGCTCATTAAAATCGCCGAGGGCTTGCTGCAGCCTATGAT AGTTTCTGCCATGTTGGAAAATGAGAGTATCCAGGGGCTGTCTGGTGTGAGACCAACTGGTTACCGGAAG CGCTCCTCCAGCATGGTGGATGGAGAGAATTCGTATTGCCTGGAGGCCATCGTCCGCCAGATGAATTCTT TCCATACAGTCCTGTGTGACCAGGGTCTGGACCCCGAGATTATCCTGCAGGTGTTCAAACAGCTCTTCTA CATGATCAACGCTGTGACTCTTAACAACCTACTCCTGCGGAAAGACGCCTGCTCCTGGAGCACAGGCATG CAACTCAGGTACAACATAAGTCAACTGGAAGAGTGGCTTCGGGGCAAAAACCTTCACCAAAGTGGAGCAG TTCAGACCATGGAGCCCCTCATCCAGGCAGCCCAGCTCCTCCAGCTGAAGAAGAAAACCCACGAGGATGC TGAGGCCATCTGCTCTCTGTGCACCTCCCTCAGCACCCAGCAGATTGTCAAAATTTTAAACCTCTACACT CCCTTGAATGAATTTGAGGAACGGGTCACAGTGTCCTTCATACGAACAATCCAGGCTCAGCTACAAGAGA GGAATGACCCTCAGCAGCTCCTGCTGGACTCCAAGCACGTGTTCCCAGTTCTGTTTCCATATAACCCATC TGCTCTGACCATGGACTCGATCCACATCCCGGCCTGTCTCAACCTGGAGTTTCTCAATGAAGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_201600
Insert Size	5457 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_201600.2, NP_963894.1
RefSeq Size	6647 bp
RefSeq ORF	5457 bp
Locus ID	17919

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