Brwd3 (NM_001081477) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Brodl; D030064D06Rik; Gm596
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224940 representing NM_001081477 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCACCTACCCAGATCGAAGCCGAGCTGTATTACCTGATCGCAAGGTTCTTGCAGTCCGGAC CCTGTAACAAATCCGCTCAGGTGCTAGTGCAAGAGCTCGAAGAGCATCAGCTGATTCCGCGCCGCTTAGA CTGGGAAGGGAAAGAGCACCGAAGAAGCTTCGAGGATCTGGTGGCAGCCAATGCACACATTCCTCCAGAC TACCTCCTTAAAATTTGTGAGAGGATTGGCCCTTTATTAGATAAGGAAATCCCTCAGAGTGTTCCTGGGG TACAGACATTACTAGGTGTCGGTCGGCAGTCTCTGTTACGGGATGCCAAAGACTGTAAGAGTACACTATG GAATGGGTCTGCTTTTGCAGCCCTCCACAGAGGCAGACCCCCAGAACTACCTGTAAATTATGTGAAACCT CCAAACGTGGTGAATATCACCTCAGCCAGGCAATTAACCGGATGTAGTCGCTTCAGCCATGTTTTCCCCT CATCTGCTTACCAGCACATTAAGATGCATAAGAGAATTCTGGGACACTTATCTTCTGTCTACTGTGTAGC CTTTGATCGAAGCGGGAGGCGAATTTTTACAGGTTCAGATGACTGTTTGGTGAAAATCTGGGCAACAGAT GATGGACGCCTCCTTGCCACACTGCGAGGGCACTCTGCTGAAATCTCCGACATGGCTGTTAATTATGAAA ATACTCTCATTGCTGCAGGCAGCTGTGATAAAGTTGTGAGAGTATGGTGTCTTCGTACTTGTGCACCAGT TGCTGTCCTTCAGGGACATTCAGCTTCTATTACTTCCATACAGTTTTGTCCATCAACTAAAGGCACAACT AGATACCTCACTTCTACTGGTGCTGATGGAACAATTTGTTTCTGGCAATGGCATGTAAAAACAATGAAGT TTAGAGATCGCCCAGTAAAATTTACTGAGAGATCCAGACCTGGAGTTCAGATATCTTGTTCATCTTTCAG TTCTGGTGGTATGTTCATTACAACAGGGAGCACTGATCATGTAATTAGGATATACTATTTGGGTTCTGAG ATACCTGAGAAAATTGCTGAATTAGAATCACATACGGACAAAGTTGTTGCTGTTCAGTTCTGTAACAACG GAGACAGCTTAAGGTTTGTTAGTGGAAGTAGAGACGGAACAGCAAGAATATGGCAATATCAACAACAAGA GTGGAAGAGCATAGTGCTAGATATGGCTACTAAAATGAGTGGGAATAATTTGACATCAGCTGAAGACAAA GTCACTAAACTAAAGGTGACTATGGTGGCTTGGGATCGGTATGATACCACTGTTATTACTGCAGTGAACA ATTTCCTTTTGAAAGTTTGGAATTCTGTCACAGGGCAGCTTCTTCATACTTTATCTGGGCATGATGATGA AGTATTTGTTCTAGAAGCACATCCATTTGATCAAAGGATCATACTTTCAGCAGGTCATGATGGGAACATT TTTATTTGGGACCTTGACCGGGGGACCAAAATACGAAATTACTTTAATATGATCGAAGGCCAAGGTCATG GTGCAGTATTTGATTGTAAATTTTCACCAGATGGAAACCATTTTGCCTGTACAGACTCTCATGGGCATTT GTTGCTATTTGGCTTTGGATGCAGTAAATACTATGAAAAGATTCCAGATCAGATGTTCTTCCATACCGAT TATCGGCCCCTCATCAGAGATGCCAATAACTATGTATTAGATGAACAAACTCAACAAGCTCCTCACCTCA TGCCTCCCCCATTCTTGGTGGATGTCGATGGAAATCCTCATCCTACAAAATTTCAACGGCTGGTACCAGG ACGGGAAAACTGTAAAGATGAACAACTTATTCCACAGTTAGGATATGTGGCTAATGGTGACGGTGAGGTG GTAGAACAGGTAATTGGGCAGCAAACCAATGACCAAGAAGAAAGCATTCTTGATGGAATAATCAGGGAGT TACAGAGAGAACAAGATCTCAGATTAATTAATGAAGGAGATGTTCCACATTTTCCAATTAATAGATCATA TTCTGTTAATGGTGCTCTGAGTAGTCCAAATATGGACATACCTTCTTCCCCAAATATTGGCCTTCGGCGT AGTGGCCAAATTGAAGGTGTTCGGCAGATGCACAACAATGCTCCCCGGAGCCAGATGGCCACTGAAAGAG ATCTCATGGCCTGGAGCCGAAGAGTGGTGGTGAATGAGCTAAATAGTGGCGTTAGTAGGGTACAAGAAGA ATGTCGAAATGCAAAAGGTGACCTAGAAGTTAGTCTTTATACTGTTGAAAAGAAGAAAAAGCCATCTTAT CCTATCCAAAGAAATGATTATCAGCCCAGCTGTGGAAGGTCTTTACGAAGGACACAACGCAAGCGACAGC ATACTTACCTAACAAGGTCGAACATAGAGCATAATTCACAGGCATCATCTCAAACTTCCGGTGTACAAGA AGATTCAGACAGCTCTTCAGAGGAAGATGAAACTGTTGGCACAAGTGATGCTTCCGTAGAAGACCCTGTT GTTGAGTGGCAAAGTGAGAGTTCTTCCAGCGATTCATCAAGTGAATATTCTGACTGGATAGCAGATGCTG GGATAAATTTGCAGCCTCCCAAAAGACAAACAAGACAGGCAACACAGAAAATATACAGCAGTTCTGAAGA TGAAAATTTGAAATTAGAAGATAGGCAGAAGAAACCTAAACAGACTAAAAAGAAGAAAGGGGGACTTGTT TCTATGGCAGGCGAACCCAATGAAGAATGGCTTGCCCCTCAGTGGATCTTGGATACCATACCACGACGTT CTCCTTTTGTTCCACAGATGGGAGATGAGCTTATTTACTTTCGACAAGGACATGAAGCTTATGTGCGAGC TGTAAGGAAATCAAAAATCTATAGTGTTAATTTACAAAAACAGCCATGGAACAAAATGGATCTCAGGGAG CAAGAATTTGTGAAGATTGTAGGAATCAAATATGAGATTGGACCACCTACGCTATGCTGCTTGAAACTTG CATTTTTGGATCCAATTTCAGGCAAAATGACTGGAGAGTCTTTTTCCATTAAGTATCATGACATGCCAGA TGTCATTGATTTCCTTGTGTTACATCAGTTTTATAATGAAGCCAAAGAAAGAAACTGGCAGATTGGTGAT AGATTCCGCAGTATAATAGATGATGCCTGGTGGTTTGGGACTGTGGAAAGTCAGCAGCCTTTTCAGCCAG AATATCCTGATAGCTCCTTCCAGTGTTACAGTGTTCACTGGGACAATAATGAAAGAGAAAAAATGAGCCC CTGGGATATGGAGCCGATTCCAGATGGAACTGCTTTTCCAGATGAAGTTGGTGCTGGTATTCCTGTGTCC CAGGAAGAACTAACTGCATTGCTCTACAAACCCCAGGAAGGAGAGTGGGGTGCTCATTCCAGAGATGAGG AGTGTGAACGAGTTATTCAGGGCATCAACAACCTTCTTTCCCTGGATTTTGCCAGCCCTTTTGCTGTTCC AGTGGATCTCAGTGCCTACCCCTTGTATTGTACTGTAGTTGCTTATCCAACTGACCTCAATACCATCAAA CAAAGACTTGAAAATCGCTTTTACAGGAGAATATCAGCATTAATGTGGGAGGTACGCTATATTGAACACA ATGCTAGGACTTTCAATGAGCCAGATAGTCCCATAGTTAAAGCAGCCAAGATTGTGACTGATGTCCTACT TCGGTTTATTGGGGATCAAAGCTGTTCTGATATTTTGGATACTTACAATAAAATCAAAGCAGAAGATCCA GACAGCAGCGATCTGGAGGAGGATTCAGAAATGGTTGACTTAGATTCAGATGGTCCTGGCACTTCATCTG GAAGGAGGGCTAAATGTCGAGGCAGACGACAGTCTCTCAAGTGTAATCCTGATGCTTGGAAAAAGCAATG CGAAGAGCTACTGAGCCTCATTTATGAACGGGAAGACTCAGAGCCATTTCGTCAGCCAGCAGATCCTCAA TCTTACCCAGTTCAACAGCAACAAGAAGGGGAATCCTCACAATCAGTTCCTCCAGATAGGCAAGACCCAT CTCTTTCTGAGGATTATCAAGATGGTATAGACACACCTATGGACTTCAGCACTGTGAAAGAAACTTTGGA ATCAGGAAATTATGATAGTCCTCTGGAATTTTATAAAGATGTTCGCCAAATATTCAGCAACTCCAAAGCT TATACTTCTAATAAAAAGTCAAGGATCTATAGCATGACGCTGAGATTGTCAGCCTTATTTGAAAATCACA TTAAAAACATCATCTCTGACTATAAGTCAGCAATCCAGAGTCAGAAGAGGAGAAGACCACGGTACAGAAA GCGTCTGAGAAGCAGCAGCAGCTCGTTATCTAGCAGCAGAGCTCCCAGTCCAAAAGGCAAACAGAAGCAA ATGAAGTTACAGCCTAAAAATGACCAGAATACCTCAGTGGCTTATGCCAGGACTAGCTCTCCTTTTTCTT CTCCTGTTTCAGATGCTGCTGAAGGTGTTTCACTGTATCTACTTGATGATGAAGGGGATGGGCCTTTTTC TCCATCAAGTTTCAGTGGATATAGTAGAAGTGGAAATAGCCATGACCCAGGGAAAGCCAAATCATTTCGT AATAGAGTTTTACCAGCTAAACAAGATCACTCCCTTGATGGACCTCTTACAAATGTTGATGGCAGAGAGC CCCGGACAGGAGCCAAAAGAAAACTGCTCAGTGCTTCAGAAGAAGATGAAAGCATGGGAGGAGAAGAGAA AGAAATGAAAGAAACAAAGGAGCAAGTACATTTATCCTCCTCTGAGAGTGGAGAGTTAGGCTCCAGTTTA AGTTCAGAAAGTACCTCTGGCTCTGACTCTGACTCTGAATCCACTTCCAGAACCGATCAAGATTATGTTG ATGGAGACCATGACTATAGCAAATTCATACAAACCAGACCTAAAAGGAAACTCAGAAAACAACACACCAA TGGAAAAAGAAACTGGAAGACGAGAGGCACAGGAGGAAGAGGCAGATGGGGAAGATGGGGAAGATGGAGC AGAGGAGGCAGAGGAAGAGGTGGCAGGGGTCGAGGGGGTCGAGGGCGAGGTGGAGGTGGGGGGAGAGGGA GAGGACGAGGGAGAGGAGGAAGAGGTGCCTCTAGAGGATCAAGCCGAGCCAAACGAGCCCGAGTTGCAGA TGATGAATTTGATACGATGTTTTCAGGACGTTTCAGTCGACTTCCACGGATTAAAACAAGAAACCAAGGC AGGAGAACTGTTTTATATAATGATGATTCAGATAATGACAACTTTGTGTCCACTGAGGATCCTCTGAATC TTGGTACATCCAGATCAGGCAGAGTACGTAAAATGACCGAAAAAGCCAGGGTTAGCCATCTCATGGGATG GAATTATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081477
Insert Size	5400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081477.1, NP_001074946.1
RefSeq Size	6434 bp
RefSeq ORF	5400 bp
Locus ID	382236
UniProt ID	A2AHJ4
Gene Summary	Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (By similarity).[UniProtKB/Swiss-Prot Function]

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