Dlec1 (NM_177117) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D630005C06Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224897 representing NM_177117 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCCGAGAGCAAGGAAGAGCAGCAATGCACAGCTTCCTCGGCCGTTATGTCGTCCTTGGAGACCC AGTTTTTGTCTGTCCCACACCAGCTCGAGTCCGCCAGTCTCAGCTGGTCCATCAGGAAGTCCTTGCATTC CTCGACCGTCTTAATGGATGATTGCCATTTCGGCTTCCCTACGCGGTCCCTCCGCGCCGGGTTCCACGCG CTCCCTCCACTCCCGGAGCCGCAGACGCTGCGTCTACGCCCCGCCTCGTTGCGCACGCAGGAAATCTCAC ATCTGCTTGCCCGAGTCTTCCGAAACTTGTACACCGCCCAGGTGATCGGAGATGACTTGAGCGACAGTTT GATCAAGGCCCGCGGCAGTGAGGATGCGCGCCACGAAGAGTTCCTTGACCAGCTGCAGCAGGCGCGAGCC ATCTATAAACAGCGTCTGGATGAAGCCGCCATGTTGGAGAGACATATCATGCAGGCCCGTGCACGGGATA TTGCAGAAACAGAACACACCACCAAACAGAGCAGACTACAGGTTGTGGAGACCCCTGTCAAACTGCCCCC AGTGAAGACAGCCTTCAGGTGGTGTCTAGACAGCAAGTTAATGCGAAAACATCACTTAATCTCTACAGAG GATTACTACACTGATCCCGTGCCGTTTTGCCCAGCACCCAAAGACAAATCCACCCTTGGATGTTCAAAGC TGACGTTTAGCTGTGAGCAGCGTTTCCTGCGCAAGTCAGAGCTGGACAGGGAGGCTGAGGAGCACTGCAG GCAGCTCACAGAGTGTGACGACTTTGAGTACACCGTGGACAGCTTGGGCTCCATCTCGAAGGCCAAAGAG AGGACCACAGATTCTGTCAAGAAGACAAGCAGGCCAAAGAATAAGAAGTGGATGGAACACCTGCAGGTGC CGCAGAGAGCCCTGGACAGGCGGCTGCTCGCCAGCATGGAGAACAGGAATCACTTCCTGAAAAACCCTCG GTTTTTCCCGCCTAACACACCACATGGAGGCAAGTCTCTCATCGTCCCTTTGAGCAAGCCAGAGAGAAGA GCATCCCTGAATGCAGAGCCTGAGTGGAGCTGTACAGATGCCCCCGTGTTCCTGGCGAAGCCATCAGTTG GATTTTTCACAGATTACGAAATTGGACCCGTTTACGAGATGGTGATCGCTCTACAGAACACCACCTCCAC CAGCCGATTCTTGCGTGTCCTCCCACCGTCCTCTCCGTACTTCGCTCTGGGACTTGGGATGTTCCCAGGA AAAGGCGGAATAGTGGCTCCCGGGATGACCTGCCAGTACATCGTCCAGTTCATCCCGGACTGTCTTGGCG ATTTTGATGATTTCATTTTGGTGGAGACACAGTCTCCCCACACGCTGGTAATCCCCCTGCAGGCCCGGAG ACCGCCGCCAGTGCTGACCTTGTCACCAGTGTTGGACTGCGGCCACTGCCTCATTGGAGGGGTGAAGATA ACCAAATTCATCTGCAAAAATGTGGGCTTCAGCGTCGGCAAATTCTGTATCATGCCCCAAAAGAGCTGGC CGCCACCGAGTTTCCGGGCTGTTGCAACTACTGGCTTTGTAGAGCAGCCTCCTTTTGGAGTAATGCCTTC CGTGTTTGAGCTGGCCCCAGGGTACGCCATACTGTTGGAGGTCTTGTTCCTCCCTACTGGCCTAGGACAG GCAGAAGAGACCTTCATCATCGTGTGTGACAACTGTCAGATAAAGGAGGTGGTCGTCGTAGGGACAGGGC AGCTGGTGGCTTTGGATCTGATTCATATTTCTGGTGGAAAAAATGAGCCAGACCCAGGAGAGCTGAAAGA TTTAACAGCCCAGTACTTTATCCGGTTTGAGCCTGAAAACGTCCAGTCCACCGCAAAGAAGCAGCTCATC ATCAGAAATGCTACACACGTGGAGCTGGCCTTCCACTGGCAGATCATGAAACCCAACCTGCAGCCTCTGA TGCCTGGAGAGACACACAGCTCAGACAGCATCAAATGCCACCCTGACAGGGAGACAGCCTTCTCCATCAT CCCCGAGAAGGGCATCCTCGAGTCCCACTCGGACCAAGAGTTCATCCTGAGTTTTTCACCTTACAAGCTC AAGTGTTTCCACAGTGTACTGCAAATGGTTCTAGAAGCTGTGCCCGAGCCCTTGAGCTCAGGGTTGGAGA ACCTGGGGGACTACTCCTACTCAGTGGATGACATGATCGTCCTGGAAATCGAGGTGAAAGGCTTGGCGGA ACCCTTCCAGGTCCTTTTAGAGCCGTACGCCCTCATCATCCCTGGGGAGAACTACATTGGCATCACCGTG AAGAAAGATTTTAAGATGTGGAACAACAGCAAGTCACCCATCAGATACATGTGGGGGAAGATCAGCCACT GCCATATCATCGAAGTGGAGCCCTGCACTGGGGTGATAGAGCCCAATGAGGTCGGAGATTTTGAGTTAAA CCTAACGGGTGGGGTGCCTGGCCCAACCAGCCAGGACCTGGAGTGTGAAATCAAAGACTCACCATGCCCG GTGGTACTCCACATTGAGGCCTCATTTAAGGGACCTGCCTTGACCATTGATGTCCCAGCCCTTCAGTTTG GTCTGCTTCGCCTGGGAAAGAAGGCTTCCATCTCCATCCAGATCCGGAATGTCAGCCAGCTGTCCGCCGT GTGGCATTTGAAGGAGAGCCCAGTCTGCCTGGCAGAGAGAGATGAGGATGTGTCCCCCTTCGACATCGAG CCTTTCTGCGGCCAGCTTCTCCCTCTGGGGGAGTGCAGAGTGACCATCACCCTTGAGGCCTCCCAGTGTC AGCGGCTGCAGACAGTCCTGGAGCTGGAAGTGGAGAACGGGTCCGGGAGCTACCTTCCCGTGTACGCTGA GGTGCAGCAGCCTCACGTGTACCTGAAGAACAGCCACTTGGAGTTCAGCAACCTCTACCTGGGCGTGTCC ACAAAGTCAAGGACCACGCTGGTCAATGGCACACTCCTGCCCACCCGGTTCCACTGGGGAAAGCTTCTGG GACCTCAAGCAGATTTGTGCACAATGACAGTCTCTCCCACACAGGGCCTGCTGGGCCCCAGTGAAGAGCT CCAACTCACCTTGGAGTTGACGACCCACACAGAGGAAAAGCTGACCAATTTGGTTCTCCTTTGTCACGTG TCTGGCATGGAGAAGCCGCTGGTCCTGGGCATCTCTGGGAAGCCCCTGGGACTTCAGGTGACCATCTCCA TAGTGGAATCTGACGGAAGCAGCACCACCAGCACTACACAGGAGGAGCTGCGCTTGGACTTCGGCTCGAA GGTGCCACTGAGGACGCCCGTGATTCGCCATATCATCCTGACAAACTGCTCCCCGATACAGACCCCATTC ACCCTCACACTGGAGTACTTCGGGAGCTCCCAGGACAGCCTGTACAAGAAGACCAGCGTCCCTGACGTGC CTCCTGCCCTGCTGAAGACAGCACGCATCCAGCAGCACGTGGCCAAGAAGGAAAGGCTGGACTTTGTGGA GAGCATGTTATCTCACAGGAAAGGAGCTGCCTTCCTTCCCCACATCTCCCAGGGCATGCTGGGAGCCTAC CAGGAGCTATGTATTGACATCACAGGCTGTGCCAACATGTGGGGCGAGTACTGGGATGAGCTCCTCTGCG TGGTGGGAGACCTGCCGGTCACTGTCATCCCGGTATACATGGCCGTGGTGGGCTGCCCTATCAGCTCCCT GAGGAATACGTGCTACAGTGTGGCCCCATTCCAGAAGGAGCCTATCACCAGGTTTGGGACCCAGATCTCT GGAGGAGATACAGTCACCAGATCTCTCCGTCTGTATAACTCCAGTCACTGTGACATCCGCCTAGACTGGG AGACCTACATCCCAGAAGAAAAGGCGGACCGGCTGCTGGAGCTGCTTGTGTTTTATGGGCCACCCTTCCC ACTGCGAGATCAAGACGGCAGTGAGATTGTGTGCCCTGAGACCTCGGAGGCCAGCTTACCAGGGTCTCCA TGCCCCTCCAATGTCTCAGTGTCTAGCCACACTGTCCCCTCAACGGTGAGCAGTGGCGGCGGCGGCGGCA CCAGGGCCGAAGAGCAGATCATGTCTGTCATCCTGCAGAGGCACGAGGCAGTGCCCGCCGGCCACATATA CCGTATCAGCCCCAAGCAGTTGGTCATCCCAGCTGGAGACCACAGGACCATCTACATCTCCTTCACGCCC ACGGTGCTCGACCCAGGGGTCATGCACAAGGTGGGGTATACTGGCTATGCCCTGGGCTTCATGAGCTTGG ATAAGGAGACAGACAGACAGATTCCAGGCCGAATGCGACGCCTTCAGGACTTTGCCGTGAGCCCCCTGAG ACTGGATCTGAATGGCCACGTGAGACACGCGCAGCTGCACGTCGAGCTGGACAGCAGTGGCTACTTGGAA TTCTGGTGCCAGGCCAGCGACCTTATCCCCCAAAATCCCTGCAGTGGGGTGCTAAGTGACCGGATTACCA CCCGCCATCTGAAACTGACCAACACCACTGAAATCCTGTATGACTTCCAGGCCCGGGTCTCCAGGCCCTT CTTCATTTCTCAAGGTGGGGCCAGCTGGGACAGCAGAGCCCCTCATCAGTGTGAAGAAGGGACGGCCTCC ACAGGCCAGTGGCTGATGATCCGTCCTCAGGACAACATGCTGGTGGATGTGTCCTTCTCACTGTCCCTGG AACTGCTGTCCTATCAGAAACTCTCGGCCGACCAGATGTTGCCCGGAGTGGATATTCAGGAGAGCGAGAA CGGAGAGAGAAAGATGGTGTTCACACAGAACCTACTCCTGGGCTTCACCAACCAGACTGTTCAGGAGGTG CCCCTGCGGGCCTTTGTGACCCTGCCAGCGCCACAGCTCTCCACCAGCTGGGTGGACTTCGGGACCTGTC TCGTGAACCAGCAGCACACCCGGCAGATCTACCTGATGAACCTCAGCTGCTGCCTGAGCTACTGGACGGT GCTGTTGGGACAAGAGGAACCAGCCAGGGAGGATCATGCGTTTGGGGTCTCCCCAGGCAGTGGACTGCTG GAGGCCCGAGCCACCAATGCACCCCCTACCTCCAGCCCACTTCAGGTGTTCTTCAACCCTAGGAGCAGCA TCCTCTATGAATCTACACTGGTGGTGGAAGGCGTGCTGAGCGAGAAGCCCTGTGTCCTGAGGCTTCGGGG CCTAGGCTCTTACGATGAAAGATACGTGAAATTCCATGAGTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177117
Insert Size	5226 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177117.3, NP_796091.2
RefSeq Size	5263 bp
RefSeq ORF	5226 bp
Locus ID	320256

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