Kndc1 (NM_177261) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2410012C07Rik; B830014K08Rik; very-kind; VKIND
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224892 representing NM_177261 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGCCATGGACCCAGCCTCCCGGGGCTTCTACGAGGAAGATGGCAAGGACTTGGACTTCTACGACT TCGAGCCGCTCCCCACCCTGCCGGAGGATGAGGAAAATGTGTCTCTGGCTGACATCCTCTCCCTGCGGGA CAGAGGCCTCAGTGAGCAGGAGGCCTGGGCTGTGTGCCTAGAGTGCAGCCTGTCCATGCGGAGTGTCGCC CACGCAGCCATCTTCCAGACCCTGTGCATCACACCAGACACCTTGGCCTTCAACACCAGTGGAAACGTGT GCTTCATGGAACAGCTCAGTGATGACCCCGAGGGTGCCTTTGTACCCCCAGAGTTTGACCTGACAGGAAA CACCTTTGAGGCTCACATCTACTCCCTGGGGGCCACACTGAAGGCTGCCCTGGAGTATGTCCCAGAACCA GAGCTGGAGCCCAAGCTGAGCACAGACCTGGAGGGACTGCTGAGCCAGATGCAGGCAGAAGACCCCAGAG AGCGGCCGGACCTGGCGAGCATCATAGCACTGTGTGAGGAGAAGATGCAGCCTGTGTCCTCATGCCGCCT GTGCCGCAGTCTCTCAGCCATTGGAAGAAGGGTGCTCTCCATTGAGTCCTTCGGAGCATTCCAGGAGCTC AGCGAGAACACATGGAGGGGAAGGCCTGCTCCAAGAAACGTGGGGCCCAAGAAGATGCCAGGAGACCTCA GCACTGACCCAGAGGCACTGTTCCCATCAAAGGGCCTACTCCAGCCTCCTGCTTCCAGGGATGCCGAGCA GGAGGCTGGGCAGAGGCCCAGAGCCCCATCCCCCAAACCACTGCTGTCAGCCCCTGTGAGAAATGGTGAG AACCCAGGACAGGAAGGGCTGGCTGACCTTGTCCTGGATGCCAGGTGCCCCCTGGGCGAGCTGGACAGAG ACAACCTCAGGCGAAGCCGGCTGAAAAAGGCTCAGACGTTTCCCAGGCTACTGCAGGAAAGCACAGAGAC CAGCACCCTCTGCCTGTCACTGAACGGCTCGAGAAACCAGCTGGCCATATCTGAGTTCTTTCCCCCAGAC CCTAGGAAGCTCTTCCTGGAAGGGAAAAATGGCCTTTCTGGTTTCAAAACACAGTCCAAAAGCAGACTGT GGCCAGAGCAGGAGCCTGGAGTCCAGTTGGATAAGACACCAGGTGCAGGCCGCAACCCACACAGGAGTCC AGGGGCCTCGGGGCAACTAGAGGCTTCATCTCCTAGCCAGGGATCTGTAGAGTACAAACCTTCACCCAGC CCTGTAGACGCTGGAGATTCAGACCATGAAGGCCACATACCTAGGAGTGAAGAGAAGATTCCAGAAGAAT CCAGACAACCTGGAAGCACAGCCACTGAACAGAGCCTGTCCCTGAAGGACCTTCTGTCTAAGCTAGGCCG GCCCTTCAGGGAGTACGAGCTGTGGGCACTGTGCCTCTCCTGCCTTAGCACTCTGCAGACACACAAGGAG CACCCAGCTCACCTGTGCCTGGACAACGTGTTGGTGGCCGAGGACGGGACAGTGTTCTTCGGACCGCCCC CTGCCAATGGCGCCTACAACTCACTCTTCTTGGCTCCCGAAGTGTCAGAAGAGAAGCTGGTGACAGAGAA GGCCTCTGTGTACTGTGTGGCTGCGGTTCTGTGGACAGCAGCCAAGTTCAGCGTACCCCGAGACCACAAA CTGGCCCTGCCACGTAGACTCAAAACACTCCTTCTGGACATGGCCAGACGCCATGCCTCAGAACGGCCAT CTGCAGCCGAGGCCATCAAGGTGTGCAGCAGCTACCTCCTTCAGCGAGGCATGGACAGCAGCAAGATTCT AGCCCACCTGCGGGCATCCACCTGCAAGGTTCACCCAGAGGAGGAGACCATCGGCCTCCAGAACGCCTTC TCAGTGGTTGAACTGAAATCCACCACGGCACCTGCTCCTGAGAGTAGTCCCGGCTTCCTGCAGGTCAGCA ATGATACCAAGCTGGTCGCTGTCCCAGGGCCTGTGCCTGGTCTGCCCCCCTGCTGCAAAGAAGCCTGTGA GCTGCCAGCAGCCTTCACCTCTGAGGCCACTCACTTTAAGCCCATAGTCCTGGCTCAGGACGCAAGTGTT ACCAGAGACCAGCTTGCCTTGCCCTCAGAGTCAAATGAGAAACCCAAAGAGGGAAGTGGTCATCTGGACA GGGAGGGAACAAGAAAGCAGGCAGCCCTGGAGCTTGTTGAGGCCACTGACCTAAAGATGTCCAACCAGCT GTCACCTGGTCCAGAGCTGCAGGGAGCAACTCCAGACCCTGATGGTGACTCAGGGTCCCCCAGCTCAGCC ACGGAATGTTCCTGCCCCCATGGCCCTGCTCTGGTCACCCAGCAAAAAGGAACATCAGGGACACCCAGCT CTCCTGCCTCTTCCCTGCCCCCTGAGCACAGGCCAGATGGTGAGGGACCACTAGGCACCACTGTGCTCCC AGGGCCCACCTCAGCCAGCCAGGGCTCTCGACACCCATGTAAGCCACCCAGGGGTAAGGCTGCAGCGAGT CCAAGCAGCCCTAGGGGCTCGGATGGTCATCCTGAGAAGCCTCGGCCAGCAGACCGCAAGCTCTGTCCAT CCAGTGTAGATACCTCATCCCCCCCTAAGATGACAGCCTGCCCCTCGCTTCAGGAAGCCATGCGCCTCAT CCAGGAGGAATTTGCCTTTGATGGCTACATGGACAACGGGCTAGAGGCACTGATCATGGGGGAGTATATT TACGCCTTGAAAGACCTCACCTTCGCCACCTTCTGTGGTGCCATATCTGAGAAGTTCTGTGACCTATACT GGGATGAGCAGCTGCTAAAGAACCTCTTCAAGGTGGTCAATGGGCCAGCCTCACCCTCTGAGAGCACTTC TGAGGAGCCTGGATCCCAGCCAGAACACTCACCCAGCAGATGCTCACTATCCAGCAAAAGGCCTTCCCTG CATGGACTCGGCAAGGAGAAGCCAGCCACGACCTGGGGCAGCGGGGGACCCTGCTCCCCCACAGCACTAT CAGATATAGACTCCGACACGCTCTCTCAGGGAAACTTTGAGGTTGGATTTCGGTCTCAGAAGTCAATAAA AGTCACGCGAGAGCAGCAACCAGAGGCCGAAGTGGGTGGGCAGCCAGGTCCAAGCCAGGACTCAACCAGC CATGCCTCGGACACAGTGGCCCGGCTAGCTAGATCTGAGGACGGTGGCCCAGCTGGGTCTCCAGGGGCAT CTGATTTCCAGAACTGCAGCCCTGGCTGGTCCAGTGCCTTCTATGAGGCTGACTGCTTTGGAGCCGATGT CTACAACTACGTGAAGGATCTGGAGAGGCAGAAGACCAACGGGCACACAGAACTGGAAGCCCAGAGTCCA GAGCTGGAGCAGCAGCTCATGATAGAGAAGAGAAACTATAGGAAGACCCTGAAGTTTTACCAGAAACTCT TACAGAAGGAAAAACGGAACAAAGGCTCTGAGGTCAGGACCATGTTGTCTAAACTTCGAGGACAGCTGGA TGAAATGAAGTCCAAGGTGCAGTTCCTGAGCCTGGTCAAGAAGTACCTCCAGGTCATGTATGCAGAACGC TGGGGGCTGGAGCCCTGTGCTCTGCCGGTGATTGTGAACATCGCAGCAGCCCCCTGTGACACACTGGATT TCAGCCCCCTGGATGAGTCTTCTTCCCTCATCTTCTACAATGTCAACAAGCACCCAGGCAGCGGCCGACA GAAGAAGGCTCGCATCCTACAGGCTGGTACACCACTGGGGCTCATGGCCTACCTCTACTCCAGTGATGCT TTCCTCGAGGGCTACGTGCAGCAGTTTCTCTACACTTTCCGGTACTTTTGCACACCGCACGACTTCCTGC ACTTCCTCCTGGACCGCATCAGCAGCACTTTATCCAGGGCTCACCAGGATCCTACCTCGACTTTTACCAA GATCTACAGGCGAAGCCTCTGCGTCCTGCAGGCCTGGGTGGAAGACTGCTACACCGTGGACTTCATAAGG AACGCGGGGCTGCTGGGACAGCTGGAGGACTTCATCTCTTCCAAGATCCTACCTCTAGATGGCACTGCCG AGCACCTGCTGGCCCTCTTAGAGGTGGGCACTGAGCGGCGGGCTGACAGTGCCTCTCGAGGTGCAGACCT GGAGGACCCTAAGGAGGCAGAGGAGGACACCAGACCCTTTAATGCCCTCTGCAAGAGGTTCTCAGAGGAT GGCATCACCCGGAAGAGCTTCTCTTGGAGACTGCCTCGGGGCAACGGGCTAGTGCTGCCACATCATAAGG AGCGCCAGTACACCATTGCATCCGCCCTGCCTAAGCCCTGCTTCTTCGAAGACTTCTATGGCCCCTATGC CAAAGCCAGTGAGAAGGGTCCTTACTTCTTGACAGAGTACAGCACCAACCAGCTCTTCACTCAGCTAACA CTACTGCAGCAGGAATTGTTTCAAAAGTGCCACCCTGTCCACTTCCTAAATTCACGAGCCCTGGGTGTCA TGGACAAAAGTGCAGCCATTCCCAAAGCCAGCTCTTCTGAGTCTCTTTCTGCCAAAACCTGCAGCCTCTT CCTACCCAATTACGTCCAGGACAAATATCTGCTACAGCTTTTAAGAAACGCAGATGATGTCAGTACCTGG GTGGCTGCTGAAATTGTGACCAGTCATACCTCCAAGCTTCAGGTGAACTTGCTGTCCAAATTCCTGCTGA TTGCAAAATCTTGCTACGAACAGAGGAACTTTGCAACGGCCATGCAAATCCTGGGCGGGCTGGAGCACCT GGCTGTGAGGCAGTCCCCTGCTTGGAGAATCCTGCCTGCGAAGATTGCTGAGGTCATGGAAGAGCTGAAA GCTGTGGAGGTATTCCTGAAGAGTGACAGCCTGTGTCTGATGGAAGGAAGGCGCTTCCGGGCCCAGCCTA CTCTACCCTCCGCCCACCTGCTGGCCATGCACATCCAGCAGCTGGAGACAGGAGGTTTCACCATGACTAA TGGAGCCCATAGATGGAGCAAACTGAGAAACATCGCCAAGGTGGCAAGCCAGGTGCACGCGTTCCAGGAG AACCCGTACACATTCAGCCCGGACCCCAAGCTACAGGCCCACCTCAAGCAGAGGATCGCCCGCTTTAGCG GCGCTGATGTCTCCATTTTAGCAGCTGATAACAGGGCCAACTTCCACCAGATCCCAGGAGAGAAACACTC ACGGAAGATCCAAGACAAGCTGAGGAGAATGAAGGCCACATTCCAGTAG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_177261
Insert Size	5229 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177261.4, NP_796235.4
RefSeq Size	7320 bp
RefSeq ORF	5229 bp
Locus ID	76484
UniProt ID	Q0KK55
Gene Summary	RAS-Guanine nucleotide exchange factor (GEF) that controls the negative regulation of neuronal dendrite growth by mediating a signaling pathway linking RAS and MAP2 (PubMed:17984326, PubMed:21385318). May be involved in cellular senescence (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.