Itsn1 (NM_010587) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA517634; AA545208; AI316805; AI839402; AI848451; Ehsh1; Ese1; Itsn; Sh3p17
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224855 representing NM_010587 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTTTCCCACACCTTTCGGTGGTAGCCTGGATGTCTGGGCCATAACTGTGGAGGAAAGGGCCA AGCATGACCAGCAGTTCCTTAGCCTGAAGCCGATAGCGGGATTTATTACTGGTGATCAAGCGAGGAACTT TTTTTTCCAATCTGGGTTACCTCAGCCTGTCTTAGCACAAATATGGGCGCTAGCGGACATGAATAACGAT GGAAGGATGGATCAAGTGGAATTTTCCATAGCCATGAAGCTTATCAAACTGAAGCTACAAGGATATCAGC TCCCCTCCACACTTCCCCCTGTCATGAAACAGCAACCAGTGGCTATTTCCAGTGCACCAGCATTTGGTAT AGGAGGGATTGCTAGCATGCCACCACTCACAGCTGTTGCTCCTGTGCCAATGGGCTCCATTCCAGTTGTT GGAATGTCTCCACCCTTAGTATCTTCTGTCCCTCCAGCAGCAGTGCCTCCCCTGGCTAACGGGGCTCCTC CCGTCATACAGCCTCTGCCTGCGTTTGCGCATCCTGCAGCCACATTGCCAAAGAGTTCTTCCTTCAGCAG ATCTGGTCCAGGGTCACAATTAAACACTAAGTTACAGAAGGCACAATCATTCGATGTCGCCAGCGCCCCT CCAGCAGCAGAATGGGCTGTGCCTCAGTCATCAAGGCTGAAATACAGGCAGTTATTCAACAGCCACGACA AAACTATGAGTGGACACTTAACAGGTCCCCAGGCAAGAACTATTCTCATGCAATCAAGTTTACCCCAGGC TCAGCTGGCTTCAATATGGAATCTTTCTGACATTGATCAAGATGGAAAACTCACTGCAGAAGAATTTATC CTAGCTATGCACCTAATTGATGTTGCCATGTCTGGTCAGCCACTGCCGCCCGTCCTGCCTCCAGAATACA TCCCTCCTTCCTTCAGAAGAGTTCGCTCCGGCAGTGGGATGTCCGTCATAAGCTCTTCTTCTGTGGATCA GAGGCTGCCTGAGGAGCCGTCGTCAGAGGATGAGCAGCAGCCAGAGAAGAAACTGCCTGTGACATTTGAA GATAAGAAGCGGGAGAACTTCGAGCGAGGCAGTGTGGAGCTGGAGAAGCGCCGCCAAGCGCTCTTGGAGC AGCAGCGCAAAGAGCAGGAGCGGTTGGCTCAGCTGGAGCGCGCCGAGCAGGAGAGGAAAGAGCGGGAGCG CCAGGAGCAGGAGCGCAAGCGGCAGCTGGAGCTGGAGAAGCAGCTGGAGAAGCAGCGGGAGCTGGAGCGG CAGCGAGAGGAGGAGAGGAGGAAGGAGATCGAGAGGCGCGAGGCCGCAAAACGGGAACTGGAAAGGCAGC GACAACTTGAATGGGAACGGAACCGGAGACAGGAACTCCTGAATCAGAGGAACAAGGAGCAGGAGGGCAC CGTGGTCCTGAAGGCAAGGAGGAAGACTCTGGAGTTTGAGTTAGAAGCTCTGAATGACAAAAAGCATCAG CTAGAAGGAAAACTTCAGGATATCAGGTGTCGACTGGCAACCCAGAGGCAAGAAATTGAGAGCACGAACA AGTCTAGAGAGCTAAGAATTGCTGAAATCACCCACTTACAGCAGCAGTTGCAGGAATCTCAGCAAATGCT TGGAAGACTTATTCCAGAGAAACAGATACTCAGTGACCAGTTAAAACAAGTCCAGCAGAACAGTTTGCAT AGAGACTCGCTTCTTACCCTCAAAAGAGCCTTGGAAGCAAAGGAGCTGGCCCGGCAGCAGCTCCGGGAGC AGCTGGACGAGGTGGAGAGAGAGACCAGGTCAAAGCTGCAGGAGATTGATGTTTTCAACAACCAGCTGAA GGAACTGAGAGAGATACATAGCAAACAGCAACTCCAGAAGCAGAGGTCCCTGGAGGCAGCGCGACTGAAG CAGAAAGAGCAGGAGAGGAAGAGCCTGGAGTTAGAGAAGCAAAAGGAAGACGCTCAGAGACGAGTTCAGG AAAGGGACAAGCAATGGCTGGAGCATGTGCAGCAGGAGGAGCAGCCACGCCCCCGGAAACCCCACGAGGA GGACAGACTGAAGAGGGAAGACAGTGTCAGGAAGAAGGAGGCGGAAGAGAGAGCCAAGCCGGAAATGCAA GACAAGCAGAGTCGGCTTTTCCATCCGCATCAGGAGCCAGCTAAGCTGGCCACCCAGGCACCCTGGTCTA CCACAGAGAAAGGCCCGCTTACCATTTCTGCACAGGAGAGTGTAAAAGTGGTATATTACCGAGCGCTGTA CCCCTTTGAATCCAGAAGTCACGATGAGATCACCATCCAGCCAGGAGATATAGTCATGGTGGATGAAAGC CAGACTGGAGAGCCAGGATGGCTTGGAGGAGAGCTGAAAGGGAAGACGGGATGGTTCCCTGCAAACTATG CAGAAAAGATTCCAGAAAATGAGGTTCCCACTCCAGCCAAACCAGTGACCGATCTGACATCTGCCCCTGC CCCCAAACTGGCTCTGCGTGAGACCCCTGCTCCTTTGCCAGTGACCTCTTCTGAGCCCTCCACAACCCCC AACAACTGGGCAGACTTCAGTTCCACGTGGCCCAGCAGCTCAAACGAGAAGCCAGAAACGGACAACTGGG ATACATGGGCGGCTCAGCCTTCTCTGACCGTACCTAGTGCTGGCCAGTTACGGCAGAGATCAGCCTTTAC CCCAGCCACAGCCACTGGCTCCTCCCCATCTCCCGTCCTGGGCCAGGGTGAAAAGGTGGAAGGGCTACAA GCGCAAGCCCTGTATCCCTGGAGAGCCAAAAAAGACAACCACTTAAATTTTAACAAAAGTGACGTCATCA CCGTTCTGGAACAGCAAGACATGTGGTGGTTTGGAGAAGTTCAAGGTCAGAAGGGTTGGTTCCCCAAGTC TTACGTGAAACTCATTTCAGGGCCCGTAAGGAAATCCACAAGCATCGATACTGGCCCTACTGAAAGTCCT GCTAGTCTAAAGAGAGTGGCTTCCCCGGCCGCCAAGCCAGCCATTCCCGGAGAAGAGTTTATTGCCATGT ACACATACGAGAGTTCTGAGCAAGGAGATTTAACCTTTCAGCAAGGGGATGTGATTGTGGTTACCAAGAA AGATGGTGACTGGTGGACGGGAACGGTGGGCGACAAGTCCGGAGTCTTCCCTTCTAACTATGTGAGGCTT AAAGATTCAGAGGGCTCTGGAACTGCTGGGAAAACAGGGAGTTTAGGAAAAAAACCTGAAATTGCCCAGG TTATTGCTTCCTACGCTGCTACTGGTCCCGAACAACTCACCCTGGCTCCTGGGCAGCTGATTCTGATCCG GAAAAAGAACCCAGGTGGATGGTGGGAAGGAGAACTGCAAGCTCGAGGGAAAAAGCGCCAGATAGGGTGG TTTCCAGCAAATTATGTCAAACTTCTAAGCCCCGGAACAAGCAAAATCACCCCAACTGAGCTACCCAAGA CCGCAGTGCAGCCAGCAGTGTGCCAGGTGATCGGGATGTACGATTACACCGCCCAGAACGATGACGAACT AGCCTTCAGCAAAGGCCAGATCATCAACGTCCTCAACAAGGAGGACCCGGACTGGTGGAAAGGAGAAGTC AGTGGGCAAGTTGGGCTCTTCCCATCCAATTATGTAAAGCTGACCACAGACATGGACCCCAGCCAGCAAT GGTGCTCAGACCTGCATCTCTTAGATATGCTGACCCCGACTGAGAGGAAGCGGCAAGGCTACATCCATGA ACTCATTGTCACGGAGGAGAACTACGTGAACGACTTGCAGCTGGTCACAGAGATCTTTCAGAAACCCCTG ACGGAGTCTGAGCTGCTGACAGAAAAAGAGGTTGCTATGATTTTTGTTAACTGGAAGGAGCTGATCATGT GTAATATCAAACTGCTGAAAGCGCTGAGAGTCCGCAAGAAGATGTCTGGGGAGAAGATGCCGGTGAAGAT GATTGGCGACATCCTGAGCGCCCAGCTGCCGCACATGCAGCCTTACATCCGCTTCTGCAGCTGCCAGCTC AATGGGGCTGCCCTCATCCAGCAGAAGACGGACGAGGCTCCAGACTTCAAGGAGTTCGTCAAAAGACTGG CAATGGACCCTCGGTGCAAAGGAATGCCTCTGTCCAGCTTTATACTGAAGCCTATGCAGCGTGTCACAAG ATACCCGCTGATCATTAAAAACATCCTGGAAAACACTCCCGAGAACCATCCAGACCACAGCCACCTGAAG CATGCCCTGGAAAAGGCGGAGGAGCTATGCTCCCAGGTGAACGAGGGAGTTCGAGAGAAGGAGAACTCAG ACCGGCTGGAGTGGATCCAAGCCCACGTGCAGTGTGAAGGCCTTTCTGAGCAACTGGTGTTCAATTCAGT GACCAACTGCTTGGGACCACGCAAGTTTCTGCACAGCGGGAAGCTCTACAAGGCCAAGAGCAACAAAGAA CTGTATGGCTTCCTCTTCAACGACTTCCTCCTGCTGACCCAAATCACAAAGCCCTTAGGCTCTTCCGGCA CCGACAAAGTCTTCAGCCCCAAATCTAACCTTCAGTATAAAATGTACAAAACGCCCATTTTCTTAAATGA GGTTCTAGTAAAATTGCCCACGGACCCTTCTGGAGATGAGCCTATCTTCCACATTTCCCACATCGACCGG GTCTACACCCTCCGAGCAGAGAGCATAAATGAGAGGACTGCCTGGGTGCAGAAAATCAAGGCGGCGTCTG AGCTCTACATAGAGACGGAGAAAAAGAAGCGAGAGAAGGCGTACCTGGTCCGTTCCCAGCGGGCGACCGG TATTGGAAGGTTGATGGTGAACGTGGTAGAAGGCATTGAGCTGAAGCCCTGTCGGTCACATGGAAAGAGC AACCCGTACTGTGAGGTGACCATGGGCTCTCAGTGCCACATCACCAAGACAATCCAGGACACGCTAAACC CCAAGTGGAATTCTAACTGCCAGTTCTTCATCAGAGACCTGGAGCAGGAGGTTCTCTGCATCACAGTGTT TGAGAGGGACCAGTTCTCGCCTGATGATTTTTTGGGTCGGACAGAGATCCGAGTGGCCGACATCAAGAAA GACCAGGGCTCCAAGGGGCCGGTTACGAAGTGTCTCCTGCTGCATGAGGTCCCCACGGGAGAGATTGTGG TCCGCCTTGACCTGCAGTTGTTTGATGAGCCGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010587
Insert Size	5145 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010587.2, NP_034717.2
RefSeq Size	13976 bp
RefSeq ORF	5145 bp
Locus ID	16443
UniProt ID	Q9Z0R4
Gene Summary	Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery (PubMed:10064583). Acts as guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex (By similarity). Plays a role in the assembly and maturation of clathrin-coated vesicles (By similarity). Recruits FCHSD2 to clathrin-coated pits (By similarity). Involved in endocytosis of activated EGFR, and probably also other growth factor receptors (PubMed:16914641). Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 (By similarity). Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways (PubMed:16914641). In chromaffin cells, required for normal exocytosis of catecholamines (PubMed:18676989). Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones (PubMed:23633571). Inhibits ARHGAP31 activity toward RAC1 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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