Kif1a (NM_001110315) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	ATSV; C630002N23Rik; Gm1626; Kns1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224829 representing NM_001110315 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGGCCTCTGTGAAGGTGGCGGTGCGTGTCCGCCCCTTCAACTCCCGGGAAATGAGCCGAGACT CCAAGTGCATCATTCAGATGTCTGGAAGCACCACCACTATTGTCAACCCCAAACAGCCCAAGGAGACACC CAAGAGCTTCAGCTTTGATTACTCCTACTGGTCACATACCTCGCCGGAGGACATCAACTATGCCTCTCAG AAGCAGGTCTACAGGGACATTGGGGAAGAGATGCTACAGCATGCCTTTGAGGGCTACAATGTGTGCATCT TTGCCTATGGGCAGACGGGGGCCGGCAAGTCCTACACCATGATGGGGAAACAGGAGAAGGACCAGCAAGG CATCATTCCACAGCTCTGCGAAGACCTCTTCTCTCGAATCAACGACACCACCAACGACAACATGTCCTAC TCTGTGGAGGTCAGCTACATGGAGATTTACTGTGAGCGAGTCCGTGACCTCCTGAACCCCAAGAACAAAG GCAACCTGCGTGTGAGGGAGCACCCTCTGCTGGGGCCTTATGTGGAGGACCTCTCCAAGCTGGCTGTAAC CTCCTACAATGACATCCAGGACCTCATGGACTCAGGGAACAAGGCCAGGACTGTGGCGGCCACAAACATG AATGAGACCAGCAGCCGTTCTCACGCAGTCTTCAACATCATCTTTACCCAGAAGCGCCATGATGCAGAGA CCAACATCACCACAGAGAAAGTGAGCAAAATCAGCCTGGTGGACCTGGCAGGCAGTGAACGAGCCGACTC CACAGGAGCCAAGGGCACGCGACTCAAGGAGGGGGCCAACATCAACAAGTCTTTGACCACCCTGGGAAAG GTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAGAAAAAGAAGAAGACAGATTTCA TCCCTTACCGAGACTCCGTATTGACATGGCTTCTCCGGGAAAACCTCGGTGGCAACTCAAGGACAGCCAT GGTGGCAGCCCTGAGCCCTGCGGACATCAACTATGATGAGACCCTCAGCACACTGAGATATGCAGATCGG GCCAAACAAATCCGCTGCAATGCCATCATCAATGAAGACCCCAACAACAAGCTGATTAGAGAACTGAAGG ATGAGGTGACCAGGCTGAGAGACCTGCTGTATGCCCAAGGTCTTGGCGATATCACTGACATGACCAATGC CCTGGTGGGCATGAGCCCCTCATCTTCACTCTCAGCCCTGTCCAGCCGTGCAGCCTCTGTGTCCAGTCTC CATGAGCGCATCTTGTTTGCTCCAGGCAGCGAGGAGGCTATTGAAAGGCTGAAGGAAACAGAGAAGATCA TTGCTGAACTCAATGAGACCTGGGAGGAGAAGCTGCGGCGGACAGAAGCCATCCGGATGGAGAGGGAAGC CTTGCTGGCTGAGATGGGTGTGGCCATGAGGGAAGATGGTGGTACCTTGGGTGTGTTTTCTCCCAAAAAG ACTCCTCATCTTGTCAACCTGAATGAGGACCCTTTGATGTCCGAATGCCTCCTTTACTACATCAAAGATG GCGTCACCAGAGTGGGCAGAGAAGATGCAGAGAGGCGGCAAGACATTGTCCTGAGTGGACACTTCATCAA AGAGGAGCACTGCATCTTCCGGAGTGACTCCCGAGGAGGCGGAGAAGCTGTGGTGACCCTGGAGCCCTGT GAAGGAGCAGACACATATGTCAACGGCAAGAAAGTCACTGAGCCCAGCATCCTACGGTCAGGAAACCGCA TCATCATGGGAAAAAGCCACGTGTTCCGGTTTAACCACCCTGAGCAGGCCCGTCAGGAGCGTGAGCGAAC ACCTTGTGCAGAGACACCTGCTGAGCCAGTGGACTGGGCCTTTGCCCAGCGGGAACTGCTGGAGAAGCAA GGCATTGACATGAAGCAGGAGATGGAGCAGAGGCTCCAGGAGTTGGAGGACCAGTACCGGAGGGAGCGGG AGGAGGCCACATACCTTCTGGAGCAGCAGAGGCTGGACTATGAGAGTAAGCTGGAGGCCCTACAGAAACA GATGGACTCCAGGTACTACCCAGAGGTGAACGAGGAGGAAGAAGAGCCAGAGGATGAAGTCCAATGGACA GAACGAGAATGTGAGCTGGCATTGTGGGCCTTCCGGAAGTGGAAGTGGTACCAGTTTACTTCTCTGCGGG ACCTGCTGTGGGGCAATGCCATCTTCCTGAAGGAGGCCAACGCCATCAGTGTGGAGCTAAAGAAGAAGGT CCAGTTCCAGTTTGTCCTCCTCACGGACACCCTGTACTCCCCACTGCCGCCAGACCTACTGCCCCCAGAA GCCGCCAAAGACCGAGAGACACGACCCTTCCCCAGGACCATTGTGGCAGTGGAGGTCCAGGACCAGAAGA ATGGGGCCACCCACTACTGGACACTGGAGAAACTCAGGCAACGCCTGGACCTGATGCGGGAAATGTACGA CAGGGCTGCAGAGGTTCCCTCCAGCGTCGTTGAGGACTGTGACAACGTGGTGACCGGCGGGGACCCCTTC TATGACCGCTTCCCCTGGTTCCGACTGGTGGGCAGGGCCTTCGTATACCTGAGCAACCTGCTGTACCCTG TGCCCCTGGTGCACCGAGTGGCAATCGTCAGCGAAAAGGGAGAGGTGAAAGGCTTCCTCCGTGTGGCGGT GCAGGCCATCTCAGCTGATGAAGAGGCTCCGGATTATGGCTCGGGTGTCCGTCAGTCAGGAACTGCTAAA ATCTCCTTTGATGACCAGCATTTTGAAAAGTTCCAATCTGAGTCCTGCCCTGTGGTGGGGATGTCCCGCT CAGGAACTTCCCAAGAGGAGCTGCGCATTGTAGAAGGCCAGGGTCAGGGGGCTGATGCTGGGCCTTCAGC TGATGAAGTTAACAACAACACCTGTTCAGCTGTGCCTCCTGAAGGCCTCATGGATAGCCCAGAGAAGGCT GCCCTGGATGGGCCTCTGGACACAGCCCTGGACCACCTCCGTCTAGGCAGTACCTTCACCTTCCGGGTGA CAGTCCTGCAGGCGTCCAGTATCTCTGCTGAATACGCTGACATCTTCTGCCAGTTCAACTTTATCCACCG CCACGATGAGGCCTTCTCCACTGAACCCCTGAAGAACACTGGCAGAGGACCCCCTCTTGGCTTCTACCAT GTCCAAAATATTGCGGTAGAGGTGACCAAGTCCTTCATCGAATACATCAAGAGCCAGCCCATTGTGTTCG AGGTCTTTGGGCACTACCAGCAGCACCCGTTCCCACCTCTTTGCAAGGATGTCCTCAGCCCCCTGAGACC CTCACGTCGCCACTTCCCCAGGGTCATGCCGTTATCCAAGCCAGTGCCCGCCACTAAGCTCAGCACGATG ACACGGCCTTCCCCAGGTCCCTGCCATTGCAAGTATGACCTGCTGGTCTACTTCGAGATCTGCGAGCTGG AAGCCAATGGCGATTACATCCCTGCTGTGGTGGACCACCGTGGTGGCATGCCGTGCATGGGGACTTTCCT TCTCCATCAGGGCATCCAGAGACGGATAACTGTGACACTGTTACATGAGACAGGAAGCCACATCCGCTGG AAGGAAGTGCGCGAGCTAGTTGTGGGTCGCATCCGAAACACCCCAGAAACTGACGAAGCTCTGATTGACC CCAACATCTTGTCCCTCAACATCCTCTCATCAGGATACGTCCATCCAGCCCAGGATGACCGGACCTTCTA CCAGTTTGAAGCAGCCTGGGACAGTTCCATGCATAACTCTCTCCTGCTGAATCGTGTCACCCCTTACCGG GAGAAAATCTACATGACGCTCTCTGCCTACATTGAGATGGAGAACTGTACCCAGCCAGCTGTCATCACCA AGGACTTCTGCATGGTTTTCTACTCTCGGGACGCCAAGCTACCCGCCTCACGCTCCATTCGCAACCTGTT TGGCAGTGGCAGCCTGCGGGCCACAGAAGGCAACCGTGTGACTGGTGTGTATGAGCTCAGCCTTTGCCAC GTGGCCGACGCAGGCAGCCCAGGGATGCAGCGTCGGCGCCGGCGGGTGCTGGACACATCAGTAGCCTATG TCCGGGGTGAGGAGAACCTGGCTGGCTGGAGGCCCCGAAGTGACAGCCTGATCCTAGATCACCAGTGGGA ACTGGAGAAACTGAGCCTCTTACAGGAGGTGGAAAAGACCAGGCACTACCTGCTCCTTCGGGAGAAGCTG GAGACGACCCAGCGCCCTGGCCCTGAGGTGCTGTCCCCAGCCTCCAGTGAGGACTCTGAGTCCCGTAGTT CTTCAGGTGCCTCTTCCCCATTGTCAGCCGAGGGTCAGCCGTCACCTCTGGAGGCTCCCAACGAGAGGCA GAGGGAGCTGGCTGTCAAGTGCTTACGACTCCTCATGCACACATTCAACAGGGAATACACCCACAGCCAC GTCTGTATCAGCGCCAGCGAGAGCAAGCTCTCTGAGATGTCTGTCACCCTGATGCGGGACCCATCCATGT CCCCTCTGGGTGCTGCTACACTCACTCCCTCCTCCACTTGCCCCTCTCTGATCGAAGGACGATACGGTGC CACTGACGTGAGGACCCCCCAGCCCTGCTCCCGGCCCGCCAGCCCAGAGCCCGAGCTCCTGCCAGAACTT GACTCTAAGAAGACTCCCTCCCCTGTTCGGGCAACAGAGACAGAGAAGGAGCCCCAGCGACTGCTGGTCC CTGACATCCAAGAGATCCGAGTCAGCCCAATCGTCTCCAAGAAGGGATACCTGCATTTCCTGGAGCCACA CACGGCTGGCTGGGCCAAGCGCTTTGTGGTGGTGAGGCGTCCCTACGCCTACATGTACAACAGTGACAAG GACACGGTGGAGAGATTTGTGCTCAACCTCTCCACTGCGCAGGTGGAGTACAGCGAGGACCAGCAGGCCA TGCTCAAGACACCCAACACATTCGCTGTGTGCACGGAGCACCGAGGCATCCTGCTGCAGGCCAACAGTGA CAAGGACATGCACGACTGGCTGTATGCCTTCAACCCGCTCCTGGCTGGGACCATCCGGTCCAAGCTCTCT CGAAGGAGATCTGCGCAGATGCGGGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001110315
Insert Size	5070 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001110315.2, NP_001103785.1
RefSeq Size	8243 bp
RefSeq ORF	5070 bp
Locus ID	16560

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