Abca14 (NM_026458) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700110B15Rik; 4930539G24Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224826 representing NM_026458 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCACTACAGATGAAGCAGTTTTCAGTCCTCCTGTGGAAAAACTTCCTTCTAAAGAGCCGGAATG TGGTTGGCTTAGTGGTGGAGATCATCTTGATATTTCTGTTGTTTGTATGGACTTTGACTGTACGCAGAAT TTCAAAGAAGACATCTATAAGTGATGTGATTTTCAATCCTATTCTCTTGACATTGCCTAAATTCCTTAAT GAAAACTTTGACTATGAATTGGCTTATGTGCCTTCTGAGAGTAATGCAGCAAGAAATATTACTGAGATGG TGAAAAAGGATTTAAATTTCAACTTTAAAGTTCAAGGCTTTCCTTCGGAAGAATCTTTTGAGAGATACAT TAAGTATGAGAACAAGGATGCGCATGTGCTGGCTGCTATTGTGTTTGATCACAAGTTCAAAACCAGCAAT GAACGCTTGCCACTTCAGGTAAAATATAGTTTGCGCTTCGGTCGTATCTATGATCCTGAAAATTTGTTTC AGCCGTCTAAGTATCAGAAAGAAATAGAGTGGAATACATCAACTCTCTTCCCATCCGTGCCTTCTCTAGG ACCCCGGAACTTCTTGGAAAACGATGGGGGAAACCCTGGATACATCAGAGAAGGATTTCTTATCGTGCAG CATTCAGTAGACAAGGCCATCATGATGTATCACAGTGGCAGAGCAGCTGAAGACATATTTGCCAACACCA CAATTTATGCGGAGAGATTCCCACACCCGGCCTTTATTCATGACAGCTTCTTGTGGACTTTTATTGCTAT GTTTCCTTGGACAATTTTATTTACATTTACTCAAATGGCACTTGTCATAGTTGGGACCATTATGCTGGAA AAGGAAAAGAGATTAAAGGAATACCAGCTCATGGTGGGACTCAGTAATGCTATGCTCTGGGTTTCCTACT TCATCACCTTCTTATTGATGTATTTCATCATCATCTGTTTGCTGTGTGGGATTTTATTCCTCAAGATTAC ACACGAGAGAGTCTTCCAGCATAGTGACCCGCTCTTCATCGCCTTCTATTTCATGTGTTTTGCCGTCTCC TCAGTGCTGCTCGGCTTCCTGATTAGCACACTCTTTAATAAAGCCTCTCTGGCCACTTCCATTGCAGGGT TCCTCCACTTCCTCACCTTCTTTCCATATTTGATACTATATCATAAGTATGACCAGATAAGCCTCAGTGG GAAGCTGGCTCTCTGTCTCATTACCAACACGGCGCTAGCATTTGGGACTGACTTGATATGTAAGCTGGAA ATGAAGGGGCACGGCGCTCAGTGGAACAACTTTGCTACAAAAGTAAACGCCGATGATGACTTAACCTTGG CCCACATAATAGGAATGTTTTTATTTAGTGCTTTTCTGTATGGCCTTGTGGCCTGGTACTTAGACGCTGT CTTTCCAGGGACGTATGGTGTGCCCAAGCCCTGGAACTTTTTCCTGCAGAAAGCCTACTGGTTTGGAGAG CCAGCCTTAAGCAGGGAAGAGAGCCAAGTCTCTGATTTACTTTCCAGTGATTTTATGGAGCCTGAACCTG TTGGCTTAGTGGCTGGCATCCGGATTCAGCATTTGTACAAGGAATTCATTTTAAAAAACAGTACTCTGAT GGCAGTAAATGACTTGTCTTTGAACTTGTATGAGGGGCAGATAACTGTTCTTCTCGGACATAACGGGGCA GGAAAAACCACCACCTTGTCCATCCTCACAGGTTTGTATCTTCCTACCAGAGGAAAGGTCTATATTAGTG GATATGACATCTCATCTGACATGGTTCAAATTAGAAAAAGCTTGGGCTTGTGTCCCCAGGATGACCTGTT GTTCCCGATGCTGACAGTGTCAGAACACCTTCATTTCTACTGCGTGATCAAAGGAATACCTTTGCAGAAC CAATCAAGAGAAACTAACAGAATGCTCACTTCTTTTGGCCTGCTTCAGCAAAGTAATACCATGTCAAAGG ATCTGAGCGGAGGAATGAAGCGCAAGCTCTCAATCATTATAGCTCTGATAGGGGACACTAAGGTGGTGAT CCTTGATGAGCCAACATCTGGCATGGATCCAGTCTCCAGGAGGGCCACCTGGGACCTACTTCAGCATTAT AAAAAGGACCGTACCATCTTACTGACCACACACCATATGGATGAGGCCGATGTGCTTGGTGACCGGATCG CCATCCTGGTCATGGGGATCCTGAAGTGCTGTGGCTCTTCGCTTTTCCTGAAGAAACTATATGGTGTGGG GTATCACCTAGTCATCGTGAAGACTCCTGACAGCAATGATGAAAAAATCTTCCAGCTGATTAAGAATTAC ATACCGACTGCCAAGATGGAGACCAATGTTGCAGCTGAGCTGTCCTTTATCTTGCCCAAGGAGCACACAC ACAGGTTTGCAGAGCTTTTCACAGACCTAGAAGAAAAACAAGAAGAGCTAGGAATCTCTGGCTTTGGTGT CTCCATGACTACCATGGATGAGGTTTTCTTCAAGGTCAGTAATCTGGAAGATTTGAAATTGAACACTGAG ATTGCTCCATCAGCTTCCATAGTGAGCCAAAGCTCAAATGAAGACAACCAGAACATGAATGTGCCCAGAA ATTTTGAGCGACCAGGTTACTCAAGAAGACACTTGGATTCTTCATTCAATGCCGGGTGGTCTCTTTACAT CCAGCAGTTCAGAGCCATGTTCATCAAAAGGGTAATGTTCTCTTGGCGCTACTGGAAACTGCTGCTGTTG CAGTTGCTGGCACTTCTGGGTCTCATGTATCTTCTGATAAAAGGAATAAGTTTCTCAGTTCCTAAAGAGC CTGCCAGGGTGATGGACTTGGAACAGTATGGCGAGACCATTGTTCCTTTCTCAGTTTCTGGGGACCCCAA TTTGACCCAAATTCTCACCAAAAATCTTGAGACCATGTTGAAGGCTAAAAAGCAAAAAATTCATGAAGTA CAAGGTGATATGAAGAACTACTTGCTGACAAGTAAAGACTGCATTTACTCGTGCATCGTTGCCTTTTCCC TTGATGTTACCACAGAAGAAAAGACATTTATCTTTTGGTTTAACAATGAGGCATACCATGCAGCACCGTT ATCTCTGTCGATATTAGACAACATTATTTATAAGTATCTCTCTGGCCCTGATGCCACCATTACAGTCTCC AACAACCCTCAGCCTCAACGTGTCACCAAAGACAAATCTAATGAAAGAAGCATATCAGGAATCCAGATAG TTTTTAACTTGCTCTTTGGGATGAGTATCTTCACCAGTGGCTTTTGCCTCATGACAGTGACTGAGAGAGT TAGCAAAGCAAAGCACATACAGTTTGTGAGTGGTGTCTATACCTTGAATTTTTGGCTCTCCGCTTTGCTG TGGGACCTCATCATCCACTTCGTGGCCTGTGTCTTGCTGCTGGTAGTGTTCCTATACACTGATGTGGATA TATTGTTGGAGAAATACCACTTCCTGGACACAATGTTCATCCTCATGTTGTTTGGCTGGTCTATTATCCC CTTCATCTACCTACTAAGCTTCTGGTACAACAACAGCACCAATGCCTACATCAAGATCTTTGTCTTCAAC CACTGTTTAGGGTTTATCAGTATAATTGTGGATGCTGTGGTACAAATCATACCAGATATTAAAACCAGTA CTAAGAATCTCATACTTAACTCCATGCTGCTGTTACCCATTTATAACTTTGGGATGAGTATCTACAAATA CTATAACATCCACGAGATAAGGAAATTATGTTCTTCTTTGGGATATATTAATACCATTCCAAGTTGCAAA AGTCAAATTATTGACATTCCTATATACAGTATGAATCAAAAAGCAATCGGAAGGCATGTGACTGCCATGG CTGCCACAGGACTGATATACTTTATCCTCATTATCCTTCTGGAGACTACTTCATGGAATTTAAAGATCTT CATATATCGATATGTTCTCTTTGGCATCTACAGGATATTTTATAAGGCTAGAATGTCTGAGGAGTTATCT GGATACTCAGAAGAGGAAGATGTACAAAATGAAAGAGAGACTATCCTGAACCATCCTTGGCGCTCACTAA ATTCTACTGTGCTCATTAAAGAGCTCATAAAGATATATTTTAAAATTCCACCTACTCTGGCTGTGAGAAA TATCTCTGTCGCCATTCAAAAGGAGGAGTGCTTTGGACTGCTGGGATTAAATGGAGCTGGGAAAACCACT ACCTTTAAAATTCTGACTGGAGAAGAGATAGCTACCTCTGGGGATGTGTTCATTGAAGGCTACAGCATCA CCAGAAATATTTTAAAGGTAAGGTCAAAAGTTGGCTACTGTCCGCAGTTTGATGCCTTGCTGGATTACAT GACCTCTCGGGAGATATTGACAATGTATGCCAGGGTGTGGGGGATTCCAGAGAACAGTATTAGAGCCTAT GTGGACAACTTGTTGAAAATGCTGTATCTGAAACCACAGGCTGATAAGTTTATTTACACTTTGAGTGGAG GAAACAAACGTAGGCTGAGCACTGCCATTGCCATAATGGGAAACTCTACAGTTGTCTTCCTAGATGAGCC ATCCACTGGCATGGACCCACTAGCCCGGCGCATGCTCTGGAACGCAGTGATAAAGACAAGGGAGAGTGGC AAGGTCATCATCATAACCTCTCACAGTATGGAGGAGTGTGAAGCTCTCTGTACCAGGCTAGCCATCATGG TGCAGGGGAAGTTTGTGTGCCTGGGAAGCCCTCAGCATCTCAAGAACAAATTTGGCAATATTTACACCAT GACCATCAAGTTCAAAACTGACACCGATGACAACACGGTACAAGATTTAAAAGATTTTATTGCCGAAGTT TTCCCAGGTAGCGACTTAAAGCAAGAGAATCAAGGGATCCTTAATTACTACATTCCCAGCAAGAACAACA GCTGGGGAAAGGTGTTTGGCATTCTGGAGAAAGCGAAGGAGGACTACAATTTGGAAGATTATTCCATCAG TCAGATCACGCTGGAGCAAGTCTTCCTGACCTTTGCCAATCCAGATAACCCACGGAGCGGCTATGAGAAA GCAGTGCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_026458
Insert Size	5052 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_026458.4, NP_080734.3
RefSeq Size	5384 bp
RefSeq ORF	5052 bp
Locus ID	67928

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