Alpk3 (NM_054085) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW319487; D330016D04; MAK; Midori; mKIAA1330
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224820 representing NM_054085 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCGCGGAGGGCCGCGGGCCGGGGCTGGGGCCTGGGTGGTCGAGCAGGAGCTGGTGGAGATAGCG AGGACGACGGGCCGGTGTGGACGCCCGGCCCAGCCAGTCGCAGCTACTTGCTTAGCGTGCGGCCAGAGGC TAGCTTATCAAGCAACCGGTTGTCTCACCCCAGCTCTGGAAGGAGCACCTTCTGCTCCATCATTGCTCAG CTCACAGAGGAGACCCAGCCACTGTTCGAGACCACACTCAAGTCCCGTGCCGTGTCCGAAGACAGTGACG TCAGGTTCACCTGCATTGTCACAGGATACCCAGAGCCAGAGGTGACCTGGTACAAGGATGACATAGAACT GGACCGTTACTGTGGCTTGCCAAAATACGAGATCACTCATCAAGGCAACCGTCACACCCTGCAGCTGTAC AGGTGTCAGGAAGAAGATGCTGCCATCTACCAAGCCTCTGCCCGGAACACCAAGGGCATCGTGTCCTGCT CAGGGGTCCTAGAGGTGGGCACTATGACGGAGTACAAGATTCACCAGCGCTGGTTCGCCAAGTTGAAGCG CAAGGCTGCAGCCAAGATGAGGGAGATTGAGCAGAGCTGGAAGCATGGAAAAGAGGCTTCAGGGGAGGCT GACACGCTTCTTCGCAAGATCAGCCCCGACCGCTTCCAAAGAAAGCGCCGACTGAGTGGAGTCGAAGAGG CTGTCCTCTCCACGCCAGTCAGGGAAATGGAGGAAGGCTCCTCAGCGGCTTGGCAGGAAGGAGAGACTGA GTCTGCTCAGCACCCGGGGTTGGGTTTGATCAACAGTTTTGCTCCTGGAGAGGCGCCCACCAATGGGGAG CCTGCTCCAGAGAACGGGGAAGACGAAGAGCGTGGCTTGCTGACATACATCTGCGAGGTCATGGAACTGG GGCCTCAGAACAGCCCTCCAAAGGAGTCTGGGGCTAAGAAGAAAAGGAAGGATGAGGAATCTAAACCAGG AGAGCAGAAGCTGGAGTTAGAAAAGGCAGAAGGGAGCCAGTGCTCTTCAGAAAACGTCGTCCCCAGTACA GACAAACCCAACTCCAGTAGAAGGGAGAAGTCCACGGATACACAGCCAGCTCAGACCCAGCCCAGAGGCC GGGTAGCACGGGGGCCTGGGATAGAAAGCACCAGGAAGACAGCCTCTGTCCTGGGCATTCAAGACAAGGT CCAGGATGTCCCCGCCCCCGCCCCTGCCCCGGTCCCCGCCCCAGCCCTGGCCCCGGCCCCTGTCCCAGTC CCTGCACCCACCCCTGTTCCAAGCCGCAGCTCAGAGCAGGTGTATTTCTCCCTGAAGGACATGTTCATGG AGACCACCCGGGCAGGCAGGTCCCAGGAAGAGGAAAAACCTCCACCTCCAAGTACCAGGGTAGCTGGAGA AAGTCCCCCAGGAAAGACACCAGTCAAGTCTAGACTGGAGAAGGTACCGATGGTCTCCAGCCAGCCCACA TCTTCCATGGTTCCCCCGCCCATTAAGCCTTTGAACAGGAAGAGATTTGCCCCTCCTAAATCCAAAGTGG AGTCAACTACTACCTCTCTCTCAAGTCAGACTTCAGAATCTATGGCCCAGAGCTTAGGGAAGGCTCTACC TTCAGCCTCTACCCAGGTCCCAACACCCCCTGCTCGACGGAGACACGGCACCCGAGATAGCCCCTTGCAA GGACAAACGAGCCACAAGACTCCAGGAGAGGCTCTGGAGTCCCCAGCAACCGTGGCTCCCACCAAGTCTG CCAACAGCAGCTCCGATACCGTCTCTGTTGATCACGACAGCTCTGGAAATCAAGGGGCCACGGAGCCCAT GGATACAGAAACTCAGGAAGATGGAAGGACACTTGTGGATGGGAGAACTGGAAGCAGGAAGAAAACACAC ACAGATGGAAAGCTGCAAGTGGATGGGAGGACTCAGGGAGACGGAGCACAAGACAGAGCACACGCTTCGC CAAGGACACAGGCAGGTGAGAAGGCACCGACGGACGTTGTGACACAAGGAAGTGAGAGGCCACAGTCAGA CAGGAGTTCATGGAAGAATTTGGTGACACAGAGAAGAGTGGATATGCAGGTAGGACAGATGCAGGCAGGT GAGAGGTGGCAGCAAGACCCTGGAGACGCAAGGATACAGGAGGAAGAAAAAGAGACACAGTCAGCAGCAG GCAGCATTCCTGTAGCTTTCGAAACCCAATCAGAGCAGTTGTCCATGGCCAGCCTCAGCTCACTTCCTGG AGCTCTCAAAGGCTCACCATCAGGATGCCCTAGAGAGTCCCAGGCTATAGAATGTTTTGAGAAGAGCACA GAGGCACCCTGTGTCCAAGAAAGATCTGACTTGATGCTGCGGTCTGAAGAGGCAGCCTTCAGAAGCCATG AGGATGGGCTGCTAGGCCCCCCATCAGGGAACCGTACCTACCCAACACAGTTGCCTCCCGAGGGGCACTC AGAGCATTTGGGAGGACAAACACATCAAAGGTCAGAGCAAGAGGACAGCCTGTCTCAGTGCCCCAAGAAG GAGCAGCCCCAGGAACCGCTTCATGTGGGTCTCTCTGGTGGGCATTCAACTGGCTTGAGTCAGGAGGTGC CTGCCATGCCTTCTCTTCCTGGGACGGGCCTAACCAGTAGTCTGCAGGAGGAGCTACCAGGCACCGCAGC TTCTCTGCACACAAACACAGATGTCCCCCTCCCCTCCAGGGACCAGGACTTTCCGAGTTCTGCTCCCACT CTGCAATTGGGGCCAGGGTCCCCCACTCAGAGTCACCCACCAGAAGCCATGGCTACTAGCAGTGAGGGAG CCTGTGCCAAGGAGCCAAATGTGGACGGGAGGTCCTCAGGTACCCGGAGCTGTGACCCTGGCCTTATAGA TTCCCTGAAGAACTACTTGCTTCTGCTGCTGAAGCTATCCAGTCCAGAGACAAGTGAAGCCAGGGCCGAG TCCCAGGAAGTGGCAGACACCGGGGGCTTAACCTCCTCCTCTACTCTGGTCCCCACCATGGAGGTGGCTG GGCTGAGTCCCAGGACGTCGAGGCGAATCCTGGAACGCGTGGAGAACAATCACTTAGTGCAGAGTGCACA GACCCTGTTGCTGAGCCCCTGCACCTCCCGCCGCCTTACTGGCCTTCTGGACCGTGAGGTACAGGCTGGC CAGCAGGCTCTGGCTGCTGCCCAGTGCTCTCGGGGCCCGTGCCCCACCCCCCTCACCATCCCTGCCATTG TGGTGGGTGAGGAAGGATCTGCGGGAGAGGATTCCGAGGAGAGGACTTCGCAGGAAAGTGACAAGAAGGG ACTGCTAGGGGAAGTGGAGGGGCACACAGTGGAAAGCAGAACCCAGGAGCCCTGCCAAGAAGAAGCAATG CCAGGGGAGGCTTTGACGGGTCTCCCTGCAGCTACACCCGAGGAACTGGCTCTGGGGGCCCGGAGGAAGA GGTTCCTCCCTAAGGTCAGAGCAGGGTCAGATGGAGAGGCAAACAAGGCTGAAGAAAGGGAGAGCCCCAC GGTTTCCCCCCGGGGACCCAGGAAGGGCCTGACACCTGGGTCACCAGGGACTCCAGGGCGGGAGAGACGG TCCCCTACCCAGGCCCGAAAAGCCAGCATGTTAGAGGTGCCTGGCGCAGAAGAAGAGCCTGCAACTGGAG ACTTGGTCTCCAGATCCAAAGACAGTGGCCTGGACTCAGAGCCTGCGGTGGATGAAGGCAAGCAGGAAGC TCTGGCCAAGCAAAGGAAAGCTAAGGACCTGCTAAAAGCCCCACAGGTGATCCGGAAAATTCGGGTGGAA CAGTTTCCAGATTCTTCTGGTAGTCTGAAGCTTTGGTGCCAGTTTTTCAACATTGTTAGTGACTCAGTCT TGACATGGGCGAAGGATCAGCACCCAGTGGGCGAAGTGAACAGGAGTGCAGGGGACGAGGGGCCAGCGGC TTTGGCCATCGTGCAGGCGTCTCCCACGGACTGTGGTGTGTATCGCTGTACCATCCAAAACGAGCATGGC TCAGCGTCCACTGACTTCTGCCTCAGCCCCGAGGTATTGTCCGGCTTCATCTCCAGAGAGGAAGGTGAAG TTGGAGAAGAGATTGAGATGACCCCCATGGTGTTTGCTAAGGGTCTGGCTGACTCTGGCTGCTGGGGGGA CAAGCTCTTTGGGCGATTGGTGAGCGAGGAACTTCGAGGGGGTGGACATGGCCTTCAGAAGGCGTCCCGG GCCAAGGTCATCTATGGGCTGGAACCCATCTTCGAATCTGGCCGCACGTGCATCATCAAAGTATCCAGCC TGCTTGTGTTCGGACCCAGCAGTGAGACCTCTCTTCTGGGCAGAAACTATGACGTCACTATCCAGGGATG CAAGATCCAGAACATGAGTCGAGAGTACTGCAAAATCTTTGCAGCTGAAGCCCGGGCGGCCTCTGGCTTC GGAGAGGTGCCCGAGATCATCCCACTCTACTTGATCTACCGGCCTGCAAACAATATACCATATGCAACCC TGGAGGAAGATCTGGGCAAGCCCCTGCAGACTTACTGTTCCAGGCAGTGGGGCTGTGCTGGGGCCCCCGC AGCAGCCAGCAGCTCCGAGGCCTTGCAGAAATGCCAAACCTTCCAGCACTGGCTGTATCAGTGGACAAAC GGCAGCTTTCTTGTCACAGATCTGACAGGAGCTGACTGGAAGATGACTGATGTACAGATTGCTACCAAAC TTCGAGGATACCAAGGCCTCAAGGAGAGCTGTTTTCCTGCCCTGCTGGACCAGTTTGCCTCTTCCCACCA GTGTAACACCTACTGTGACATGCTGGGGCTGAAGCCCCTCAAAGGCCCTGAGGCTGCCCACCCTCAAGCC AAGGCCAAAGGCTCCAAAAGTCCATCTGCTGGCAGAAAAGGCTCACAGCTGAGTCCTCAACCCCAGAAGA AAGGCCTTCCCAGTCCCCAGGGCTCCAGGAAGAGCGCTCCAAGCTCCAGGGCTACACTTCAGGCCTCCCA GGCAGCCACTGTTCAGTTACTGGGACAGCCTCCTGTCCAAGATGGGAGCTCTAAGGCCCAGAGCATGCGG TAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_054085
Insert Size	5043 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_054085.2, NP_473426.2
RefSeq Size	6369 bp
RefSeq ORF	5043 bp
Locus ID	116904

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