Phrf1 (NM_001081118) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA673488
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224819 representing NM_001081118 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGACGACAACTTGGATGAGCTTGTGGCGCACAGCCCTGGGCCAGATGGACCCCCACGGGTTGGCT CCTCAGAACTGGCCAGTGATGCTGAAGAAAGCAGCAATGGCCAAAGTGGAGACTCTGAAGATGATACAGG CAGTGAGCAGGATGATGACACAGATGGGGAGGAAACAGAGGGGCTGTCTGAAGAGGAAGACCCAGAGGAT AGATCTGGTTCTGAAGACTCAGAAGATGGAGTAGAAATGGCAACGGCAGCAATAGAGACTCAAGGGAAGC TTGAAGCTAGCAGTGTACCCAATTCTGATGATGATGCAGAGAGCTGCCCCATTTGCCTCAATGCATTTAG AGACCAGGCTGTGGGCACCCCAGAGACCTGTGCCCATTATTTCTGCCTGGATTGCATCATCGAATGGTCC AGGAATGCCAACTCCTGTCCTGTTGATCGAACAGTATTTAAATGTATTTGTATTCGTGCCCAGTTTAATG GTAAAATCTTAAAGAAGATTCCAGTAGAAAACACTAAAGCTTGTGAGGCTGAGGAGGAAGACCCGACTTT TTGTGAGGTGTGTGGCAGGAGTGATCGTGAGGACCGGCTCCTACTGTGTGACGGCTGCGATGCTGGGTAC CACATGGAATGTTTGGACCCGCCTCTCCAGGAGGTGCCTGTGGATGAATGGTTCTGCCCAGAGTGTACGG TCCCTGGTGTGGATCCCACTCATGATGCAGCTCCTGTGAGTGATGAGGAGGTCTCCTTGCTACTGGCTGA TGTAGTACCTACTACAAGCAGGCTTCGGCCTCGAGTAGGCAGGACCCGGGCCATCGCTAGGACACGGCAG AGTGAGAGAGTGAGGGCAACTGTGAACCGGAACCGGATCTCCTCTGCCAGAAGGGTCCAGCATGTGCCAA GGTACCTCATGTCTTCTCTGCTGGATGAAACTATTGAGGCTGTCGCCACTGGGCTGAGCACTGCTGTATA CCAGCGTCCCTTGACACCTCGTGTCCCTGCAAAACGGAAAAGGAAGGCAGGGAGACGAAAGAAAGTGCTG GGAAGAAAGAAAACTCGGTCCAGGTCATCTGTGAAAAGTAAGAGTGGGAGTACGAGAGCCAAGAAACGTC AGCACCGTGTAAGGAAGACAAAAGGGAGGAAGCTGAAGAATGAAGTCACAGCTCGTTCTCGAATTGCACG AACACTAGGCCTCCGCAGGCCTGTCCGTGGCACCAGCATGCCATCAGTGTACAAGCCAGTGGACCCCTCC CTCGGGCTGATGAGGGCAGACATTGGAGCAGCTTCCCTGTCACTGTTTGGAGATCCCTATGAGCTGGATC CTTTTGACAGCAATGAAGAACAGTCTGCAGATCCACCTTCCCCTCTGAGTGCCAAGAGGAGAGTGCTGTC CCGCTCAGCTCTGCAGTCTCACCAGCCTGTGGCCAGACCTGTCGCCATGGGGCTCTCTAGGAGGCAGCTC CCTGCTGTGGCCCCAGAGCCCAGCGTGGAGGAGGCCCCCGTTCCTGACCTGTTGGGAAGCATCCTGTCTG GCCAGAGCCTCCTGATGATGAGCAGTGCTGATGTTGTCATACACCGGGATGGCTCTCTCAGCGCCAAGAG GGCAGCTCCTGTTTCTCTTCAGCGAAATTCTGTGACTCAATCCAGAGAAGAGTCCAGGCTCAGAGACAAC CCGCAGCCTGGGGCACTACCCTCAGAGAGTGCATCTGGTGGATTCGTGGGAGACAGACAACCAAACTCAG GGCTGAGCTGTGGGAATAGAACAGCTCTGTGCTGTCTCCCTGCCCGCATTGCCCAGACACCTGTGAGGTC AGATCCCTCACTGACTCCTCGCTCAGGTCTGTCTAGGACCCTTTCAGATGAGAACAGGCCCAGCCGGACA CACAGCAGCAGCCCCCAACTCAATGGCTCCAATGTACGGGTTAGTTCTGCCTCGACTAAGATCGTGACTC ATTCTAGCTTTCCATCGAAAAATACAGCTTCTGGGCTTCCTCAGAGAACAGGTCCCAGGAGACCTGATTT CTCCAAGCTACCCAGGATACCAAAGATCCATCGGGATGGCAATAAGAGCACGCAGGACCAGGCCCCAGCC AGCGGCCACATTGTCGAGCTCCCTAGCACTTGCATCAGCCGCCTGACTGGCAGGGAAGGCCCTGGGCAGC CAGGACGAGGCCGAGTCGACAGTGAGCCAAGCAGCAGGGGTCCCCAGGAGACTGGCTCACACACAAGTGG TTCTCGACCTCCTGCGCCCAGCTCCCATGGCAGCTTAGCCCATCTAGGGCCCTCAAGAGGAAAGGGCATT GGGTCTAGCTTTGAAAGTTTCCGGATCAACATCCCTGGGAACACTGCACATTGCAGCCAGCTGTCCAGCC CTGGATTCTGTAACACGTTCCGGCCGGTAGACAGCAAGGTGCAGAGGAAGGAGACCCCGTTCCCCCTCTT CTCCATCAAGAAGCCGAAACAACTCAAAAGCGAAATCTATGACCCCTTTGACCCCACTGGCTCGGACTCT AGCCCTCCTAGCAGCAGCCCAGAGAGTCTTGGTCCAGGCCTCCTACCCTCTGAGATCACAAGAACTATCT CCATCAACAGCCCAAAGGCCCCAGCCTTCCAGACTGTGCGCTGTGTTACCTCCTACAGGGTAGAAAGCAT CTTTGGGACTGAGATGGAACCTGAGCCCCAGCCCCCTAGTGAGCCTGTATCTGGCATGCTGGAGTTACTC AGCAAGGGATCTGCTGAGGGAACCTCTGATTTGGAGCAAGAGGGACTAGGGGAAATAGAGCCTACAGAGA TTCGGGGCTCCACAGCCCGGACACAGAGGCCACCCCCACCAGACCCTTGGGATGATGAGGACGAAGTGTC CTGTACACCCTTCTTTGGCTCTGAGGAACGGACAGTGACGTGTGTGACTGTTGAGGAGCCAGGTGTTCTA CCAAGCCCAGATGCTCCACAGATCACCACCCACAGAATTGTGGAGTTCAGGGCCTCATCCCGTTCCCGCT CCACCTCCAGCTCCCGAAGCAGGAAGAAAACGAAGAAGAAGAAGAAAAAGGTTGCCAGAGAGCACCAGAG GACACGCTCCAGCACTCGCTCTGGCTCCAGGGACAGGACTTCACGCTCAGTATCTCCAGTTGCTGAGGAA CACACCAGGAGGCACAGAGCCAAGACTAAGAGCCGGAGGTCTTCCAGTGACCGTGCCAGCAGCCAGGACA GAGCAAAGAGGAGGAAGGACAGAGACGACAGAGACAGGGAGCATAGGCGGGGTTCCTGGGGTCATGGCAG GTGCCGGCGGAAGTCCAGGTCTCGCTCAGGGAGCCCTGGCAGTTCTTCCTGTGAGCGCCATGAGAGTAAG AGACGGAAGAGGCGACATTCGGGGTCCAGGTCTCGGGGCAGCAGTCTGGAGAGGGACCGGAGACACAAAC ATCGGGAGAGGAGCCGAGAGCGAATGGACAAGCAGGAGAGTGTGACCCGGTCCCGGGAGAGGAGGAGGTG GCGATCTCGGTCGCCTAGCTTGGAGCATAGGCCACGAAGGCCACCTTCCCGTGAGAAGCGTGCCCATTCC CCAGAGAAAAAGGGGCCTGTGAGGGAGGTTTCCCCAGCCCCTGCTACACAGGGGGAGTCGAGGCAGGATG GAGACCACTCTGCTGAGCCTCCGGTTTCAGAAGTAAGTGTCTTGCCTGAGGTAGTAAGTGTCCTGCCAGA GGTGGTTGTGGCTGACCTGAACCCTCCAGAAGTCCCTCCTGTCCTGGCAGAGCCAGTTGCCCATGTGCCT GAGGACCTGGACTATGGTGAGTCTGTGGAGGCAGGCCACGTCTTTGAGGATTTTTCAAATGAAGCCATCT TCATCCAGCTCGATGACATGAGCTCGCCTCCTTCTCCTGAGAGTACAGACTCCTCCCCTGAGAGAGACTT CCCACCAAATCCCATACTGCCCCCAGCCAGCCTTCCACAAGACAGTACTTTACCTACCATCCAAAGGGAG GTGTTACCCATTCACAGTGAAGATATCTCAAAGCCTGTGCCCCAGGCCTTGGCCCCATCAGACCAGAGTC TGCTCAAGCAAGACACTGTAGAGATCACCACTACAACTCCCAGCACCCCAGCTGTGGTGCCCATGACAAA GGACAGTCCGGTGCTGAGTGCAAGAGGATGGGAAGCAGTCAGGCCCAGGGATGCTGTGGCCCAGGCCCCA CTGCTACGATCCCGAACCCTGGTGAAAAGAGTCACCTGGAACCTGCAGGAAGCAGAACACAGCACGCCAG CAGCCCTGGACAGAGATCCAAGGACACCACTTCAGAGGCCCCAGAGGCCCCAGGAAGGAGACTGGGATGC AGAGGACAGGGCCCTCATAGGATTTCAGCAGGCACCATTCTCCGAGCTGCCCCCTCCCATCCACGTGCTC CAGGAGTCTGGGTTACCTGATGCAGATCCCTCTCAGCCCCCTGGTGCTCCCAGGGCAGAAGGGCTTCCAG CTGCTGGGACTCTGCACTCAGCAGGGGGTATTCTTGCCCAGGTTTACAGCCCCAACATGCCACCACCCTT GGCTCAGCCCTCAAGCATCCTGCCCTATGCACTGGTCAGCCAGCCCTCAGTCCAGTTGATCCTGCAGGGG ACCCTTCCCCTAGCAGGCTGCGGTACAGCACAGAGCCTGGCACCAGTGCCCACAATGCCAGCTACAGTCT CAGAGCTAGCTGTTCCAACCACCAACAACTCAGAAGAAAGGACTGCTACTCCCAAAACAGCTGCTGAGAA GACCAAAAAGGAGGAGTACATGAAGAAGCTGCACATGCAAGAACGGGCTGTGGAGGAGGTGAAGCTGGCC ATTAAACCCTTCTACCAGAAGAGAGAAGTGACCAAGGAGGAGTACAAGGACATACTGCGCAAAGCCGTGC AGAAGATCTGCCACAGCAAGAGTGGTGAAATCAACCCTGTAAAGGTGGCCAACCTGGTGAAGGCCTATGT GGACAAATACAGGCATATGCGCAGACACAAGAAGACTGAAGGTGGAGAGGAGCCACCCACTCAGGGTGCT GAGACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081118
Insert Size	5049 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081118.1, NP_001074587.1
RefSeq Size	5374 bp
RefSeq ORF	5049 bp
Locus ID	101471
UniProt ID	A6H619

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