Cfap43 (NM_027559) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4632415N18Rik; 4930428C11Rik; 4930463G05Rik; AI429486; D19Ertd652e; Wdr96
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224817 representing NM_027559 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCAAGACCCGGAGCGCGACGACGTCACTGCCTCTGCCACTGCCTCTGCCTCAGCCTCAGCCCCTG CCTCTGCCTCTGCCCACTACTCCGGATCCTCCCTGTCGGTGAGATGGGTGCAAGGATTCCCTAGTCAGAA CGTCCACTTTGTCAATGACCAGACCATCTGCTACCCTTCTGGGAACTTTGTAATATTCATTAACCTGGAA ACCAAGAAGAAGACTGTACTCCAGTGTATTAATGGGATTGTGGGCGTCATGGCAACTAATGTGCCCAGTG AAGTCGTGGCTTTCTCTGACCGAAGATTTAAACCTGTTATCTACATATACAGCTTCCCATCGCTGACCAG AAAAAACAAGTTAAAAGGTGACATCCTCCTGGACTACACTCTACTTTGCTTCAGTTACTGTGGCACCTAC CTGGCCAGTTACTCGTCTCTCCCAGAGTTTGAGCTGGCCCTCTGGAACTGGGAAGCCAGTGCCATCTTGT GCAAGAAATCAAATCCCGGCATGGATGTGAGCCAGATGTCCTTCAACCCCATGAACTGGCACCAAATGTG TTTATCAAGTTCAAGTGCGATGAGCGTGTGGACCATTGAGAGAAGTAACCAGGAGCACCATTTCAGGATA AGGTCTGTCAAGCTACCCCTAGAAGACGCGACCTTCCTAAACGAGCCCGACATGCTTTTCCCCACCACTC TGCCAAAGGATCTCATTTATGGACCCGTATTGCCACTGTCGGCCATTGCCGGGCTGGTAGGCGAAGAGGC AGAGACTTTCAGGCCGAAAGATGACATATATCCTCTGCTTCACCCAACTATGCACTGCTGGACTCCGTCA AGCGACCTGTACGTTGGGTGTGAAGAGGGTCATCTTCTGATGATTAACACAGAAACCCTGAAGGTGACCG TGCTCCAGAAGGCGGAGGAGTTTCCACTTCCGGATGGAGCCCCGCTCATCAACCCACTCACGCTGGTGTA CCAAAAGGACGGCATACTCGCTTCTGGAATTGATGGTGTGATATACTCCTTCATCATCAAGGATTCAAAG TACCAAGTGAAGACCTTTCTCGAGTTTGATGGGCCGGTGACACATCTGGTGTTCTCCCCCAGTTACAAAA TGTTACTGATTCAAACAGACAAGGGGTCTGTTTATATCTATACGTTTGGTGCAGAGATGCCTCTTGATAA ACTCCTCGATGCCTGCGATGGGAAAGTGCAGGCTGTCAGCTTTATCACACCTGGAACCAAATACTTCCTG ACACTCACTAGTTCTGGGGAAGTTTCCACTGTATCACTAGAAGACTGCAATTGTACAAGCAGGATTTTCC TGAAGACTCAAGCAACAGCTCTGGCGTGCTCCCCTTCCTCGCCGACAGCTGCCGTGGGGACGGTGGATGG CTATGTCTACTTTCTGAACATCCTTGATGTGGAGTCCCCTCAGATGATCCACCAGGCCTTCCTCTCCCAG TCACCAGTGAAGATAGTGACGTATGACCAGCGAGGAATCTTTCTGTTAGTTGGTACAGAGGAAGGAAACA TCTTTGTCATCGATGCCAGACCCTCAAAGTCATTTCAAATTTTTGGATTCACTGAGACGGGCAAGGACAT CCTGCAGATATCCACGGTGTCTGTCATGGAGTCAGATGTGGTCGAAGTGCTGGTGTTGTACCCGCTTCCA GATATGGGCAGAAGCAGGCTGGAATACTTCACCCTGCCAGTCATGTTACCAGAAGTGGTTCCTGAAAACT TTTCTGATGAACGAGGAAGGCTGAAAGACGATTTAACCCACAAGTACCTGTACGAGGTGGAGCACACTCT GTCCTCTGCAGTGCTGGGCTTCACAGGCAGCAAAATCTTTGGCTTCTGCAGCCAGGTGCCCTACATCTGC AGCTATGTAATGCCCGTAAAGGAACACACTGGTGTCCTTGTTCTTAAGCCTCACCAAAAAGTACAGAGCA AACAGTACGGATCTGGAACAATCTATCTGTCTTCTCACGGATTATGGCTTATGACAATAGCTAAATGTGG AATTCTCTGTATCCGAGATATGTTCAGTATGGAAACCTTTGTTCGATGTCGGAGTCATTCTCACCAGGGG CGAGGGATCCAGAACATGAAAATGTCATTGGACGGACAGCACATCCTGGTGAATGGAAAAGATGACAACA CCCTTGTCTGCCTCAAATGGAAGCGGCTTGGAGCAAACATAGCAAGTGAGATCTTTGAACACAGCCGTCC GCTAGTGCTCCATCTGTCCCAGACAGTGGAATCGGAGAGTGTATACTTAGCTTTATCGAGAGAGTCCACG AATGAGCAGCAAGAAGAGACAACTGAATCCCAAAAACACCTGAACTCAGACTCATCAGAAGAGGAAGCAG TCATCGATCACAAGATGATTCCCTGGATCCAACAAAAAATGGAAGAGGCCATCAAAAAGGAGGTGAGGAT TTTTTCCCCTAGAAGAAAGGAGATCAAAAGAGGAATTAAGGAGCTCGCTCAGGTTATTGCAATGATGATG GAAGAGAATGAAAAGGTGGACATCATTGCAAAACTAGATGAACAGGAGTTTTGCCTGGACGCCGATGAGC TGGAAAGGCTTCATGATGAATGTGAGGAGGAAGTAGCGAAGATAAGGAAAGATGTGGAGATGCACAACTT GGCCCAGAGCTACTTAACTGAGCTCATCAAGGAGGAGTGCTGGAACTCCATGGCTGTGAAGGGCCGGGCT CTAAAGTGTTTTCATATACCCTATGTGGTTGACAACTTTCCCATGAAAGAGCGCACGGAAGAGGAGCTAC AGGAGTTGAGTAAAGTTATGCAGCAAAAGAAGACTGAGATTGAGTGTCTTAAGCTCCGGAAAGAGATTGT GGAGGTCCAGGCGACAACCACTATAGCTAAGAAACACCACGAGGAGGAGGAGGAGGAGGAAGAGGATGAA GAAAGGACAATAAAAACTACCAGTCTGCCCAATTACCTGCTTGGTAGTTTGAGCACTGATTTTGGGGCGG ACACATCTTTGTTGACAAGCCAACTGGACCTTCATTCCAGAGAGGAGAAAATCAACCAAATTATATTGCT AAAGGATATCATTTACAATATAAAAAGGAATTTCAATAGTGAATTTGATGCTGCCTATAAGCAAAAAGAG ATTGAAATTGCACGTGTAAAAGAAAAGAATGTTAGAATTGCAGAAATTATTTCAGACTTGGAATTGGAAG AAACAGTGTGGCAGCCAGTGTTTGAAGACAGTGAGAAACCAGAAAGAGCCCTTGTCGTGGAAGATGATGA GATCTCATTTAAAAAATACATTGCTCCATGGCAAAGAGCCAAAATAAAAGAAGTGGTCTCAACCTATGAA ATGGAACGGTTGCAGCAAGCACGGATCTCTGATGAACGGCAGAGAGGCCTGATGGATATGATGGGAGGTG TTCTGGAAGTCAAGAAGGAAGACATCTTAAGGATGGTGATCCCTCAACCACCTTTCATGGCGAAAGCTGA TGCTCTGTGGAGTGAAGATGAAAGGAAGCAATTCAAAGAGTACGAGAAAAAAGTCAAGGAGCTGAATGAA GAGAGAGACAAGTACAGAAAGTCTTTAGAAGCGGAGCTGAAGAAACTTCAAAATTCTATTCAAGAGAGCA CGCAGAACTTTGACGACCATCTGAAGAGACTCTTTGAAAGGAGAGTGAAGGCAGAAATGGTCATCAACCA GGAGGAACTGAAAATAAACAACATTATATTTTCGTTGCTGCTGGATGAAGAACTCAGCTCCAGGGAACAG TTCCTGAACAACTACCTTTTGAAGAAACAGGAGGAGAAAACCAAGACTGCAGAAGCCATCCAGAAGGCGA GAGAAGATCTGGACGTATTCAAGGAGCACCATGACATGTTAGTGGCAGAGGACAAAATTCTGGATCGAAG CTTCAAAAAGGAATTTTCTGACATTTTGGGTCACCAGGTGGATGTCCTCTACAAACTTTTTAAACGCCGG CCAAGGGTTCACAAACAGAAAACACAGGCAGATGTGACCAGTCTTGTTCCTTATGGAGAGCGACCAGGAT CTGCCAAGTTAAATAAGGAAAACTTAGCCCAGCTAATGAAATCCATGGATGAGTTGGACAATATCAATAA CATGCCAGAAGGCTTGGACCCCTCTGTCTGGGAACATTTCTGCTCAACTAGACGGGCAAAAGTGGAAAAT GAATACAAAGTGAAGCAGAAGGCAGCCTGCCTCCTGGAAATGACGACTTTTCTCCGGAAGCGGATGGAAG AGGATGACGTAGTGCATCATGAGATCGAAAAAGTGTTCCATGAGCTCATCCGATTACAGGATGAGAAAGT GAGATTCCAGGTGAATTTGACAGTACAAATCCTTCTTAAGCAAGGACAAGTAGAGCTGGAGAATTTCCAG TTGATGCTGGAATATTCTGACGCAATTCTCATCAATAAGAATATAATTGAAGACTTGAATTCTGTGATTC GGACTCAAGGACAAAAGAAAGTTGCTAGCATGATGGAAAGTAAAGAGGTCCACAAAGGAATCTATCAGAT TGAGTGGGAGCACAAGAAGATGGAGATGGAGATGGAGGACCTAAACCAGAGGGCCTGGGACATCGAGATG CTCTTCTTCTCACGAGACCGGCAGAAGTACCTAAACGAACCAAACTACGAGAATGTGATTGCCATTCAGA TTGGAATAATGGAGCAAACGATTAGTGTTATAGATAAGACTCATAAGAAGAATGTAGAAAACTGCAAAAA GCTACTCAAGAAACTGGGAAAATACAGCAATCAGAAAGATGTTGCAAATTACACCCTAAGCTGTAATCTA CGGGAAGAACTGGTGGCCGTCTCAGAGAGACAAGACATTTGCAATGAGATAGGGTCTAAGCTGACCTGTG AGAAGATTGCTAGAGAACGCTACGACAACCAGCTGAAGCAGCAGAAGCTGTTGAACATCTCCAAGCAGCA AGCTGAGCAGATCTCAATCCTGCAGGCTGAAGTTGAAAGACTCAGGATGAAGACATTCCCTGCTCTCATC CCAATGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_027559
Insert Size	5049 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_027559.2, NP_081835.2
RefSeq Size	5947 bp
RefSeq ORF	5049 bp
Locus ID	100048534
UniProt ID	E9Q7R9
Gene Summary	Flagellar protein involved in sperm flagellum axoneme organization and function.[UniProtKB/Swiss-Prot Function]

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