Col22a1 (NM_027174) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310067L16Rik; C80743
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224757 representing NM_027174 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGTCTTCACGAGCAGGAGAGCCATGGCTGTCCCCAGAGGGAATCCTTTGGCCTGTCTTCTGTGGA TTCTACTGCTCTGGGGAGGGGATGGTGGCTGCCAGGCCCAACGTGCAGGTTGCAAAAGTGTGCAGTATGA TCTGGTCTTCCTCCTGGACACGTCTTCTAGTGTGGGCAAGGAGGACTTTGAGAAAGTCCGCCAGTGGGTA GCCAACCTGGTAGACACCTTTGAGGTGGGCCCTGGCCACACCCGTGTGGGGGTTGTGCGCTACAGTGACC GGCCCACTACAGCCTTTGAACTGGGCCACTTCAACTCTCGCGAGGAGGTGAAGGCAGCTGCGCGGCGCAT CACCTACCATGGCGGTAACACGAACACGGGTGACGCCCTGCGTTATATCACCAGCCGCAGCTTCTCCGCC CAGGCCGGCGGCAGACCTGGGAATCGTGCCTTCAAGCAGGTGGCCATCCTGTTGACTGATGGTCGTAGCC AAGACCTGGTGCTGGATGCTGCAGCAGCTGCCCATGCAGCCGGCATCCGCATTTTTGCTGTTGGTGTGGG TGCAGCATTGAAGGAGGAACTGGATGAGATTGCCTCAGAACCCAAGTCTGCGCATGTCTTCCATGTTTCC GACTTCAATGCCATTGACAAGATCCGTGGCAAGCTGAGGCGTCGGCTTTGTGAAAATGTGCTCTGCCCAA GTGTCCGGGTAGAAGGAGATCGGTTTAAACATACCAATGGAGGAACCAAGGAAATTACAGGTTTTGACTT GATGGATTTGTTCAGTGTGAAGGAAATCTTGGGGAAGAGAGAAAACGGAGCCCAGAGTTCCTATGTACGG ATGGGTTCCTTCCCTGTGGTGCAGAGAACTGAAGATGTGTTTCCCCAAGGTCTTCCTGATGAGTATGCCT TTGTCACAACTTTCCGGTTCCGGAAAACATCACGGAAAGAAGATTGGTACATCTGGCAAGTCATTGACCA GTATGGCATCCCACAGGTCTCTATCCGGCTGGATGGTGAGAACAAGGCAGTTGAGTACAACGCTGTGGGT GCCATGAAGGATGCTGTCCGGGTGGTCTTCCGAGGCCCCCGAGTTGATGACCTCTTTGACCGTGACTGGC ACAAGATGGCTCTAAGCATCCAGGCCCAGAATGTGTCCCTCTACATTGACTGTTTGCTGGTTCAGACGCT GCCTATTGAGGAGAGGGAGAATATCGACATCCAGGGCAAGACTGTGATTGGCAAACGTCTCTATGACAGC GTTCCCATTGATTTTGACCTGCAGCGGATTGTGATTTACTGTGACTCGAGACATGCAGAACTGGAGACTT GCTGTGACATCCCGTTGGGTCCGTGTCAGGTGACTGTGGTGACAGAGCCGCCACCTGCGCCTCCACAGCT GCCCACCCCTGGGAGCGAACAGATCGGGTTCTTGAAGACCATCAACTGCTCATGCCCTCCTGGAGAAAAG GGTGAGAGGGGCTTTGCTGGCCCTCTGGGACTCCCTGGTCAGAAGGGAGATGCAGGACCCATTGGACTAA TGGGTGCTCCTGGACCCAAGGGAGAAAAAGGAGACAGTGGACGAGGACCATTCATCCATGGGGAAAAGGG TGAGAAGGGATCCCTGGGCCCACCTGGGCCCCCTGGAAGAGACGGCAGTAAAGGCATGAGAGGAGAGCCA GGAGAGCTAGGAGAGCCAGGGCTGCCTGGAGAAGTGGGCATGAGGGGGCCCCAGGGACCTCCTGGACTAC CAGGGCCTGCTGGGCCAGTTGGAGCTCCAGGTCTTCGAGGAGAACGAGGTGAAAGGGGGCCTCCAGGAGA AAAGGGGGAGCGAGGCCTGGATGGATTCCCTGGGAAGCCTGGAGAGACTGGAGAGCAGGGAAGACCTGGG CCTCCTGGTGTGGCAGGACTGCAGGGAGAGAAGGGAGATGTGGGGCCTGCAGGACCCCCTGGTGTGCCAG GCTCTGTGGTGCAGAGGGAAGGCCTGAAGGGAGAGCAGGGAGCCCCCGGACCAAGAGGTCACCAAGGCCT ACCTGGGCCTCCAGGAGCACCTGGTTTGATTGGCCCAGAAGGCAGAGATGGACCCCCGGGGCCTCCAGGT CTCCGAGGGAAGAAAGGAGAAATGGGTCCCCCAGGAACCCCTGGAGCACTGGGGCCACAGGGCCCGCCTG GACCACCCGGTGTTCCAGGGCCCCCTGGACCTGGAGGACCCCCGGGTTTACCTGGCGAGCTCGGCTTCCC GGGAAAACCTGGGCCTGCTGGGCATGCGGGGACACCTGGAAAGGATGGGCTGAATGGACCACCAGGCCTT CCTGGCTCCAAGGGAGAACCAGGAGACTCTGGCGAAAGTGGCGTACCTGGAATGCCAGGACCTCGGGGTG AAGTTGGGGAGCGGGGCCTTGCAGGTCATCCTGGTGAGAAGGGAGAAGTTGGCCTTCCGGGTGCTCCAGG CTTCCCAGGAGTACATGGAGAGAAAGGAGACCAGGGAGAAAAAGGTGAACTGGGACTTCCAGGTCTGAAA GGTGCCCGAGGAGAAAAGGGTGAGGTTGGTCCAGCAGGTCCCCCTGGCTTACCTGGAAGTCCATCAGTGT TCACACCACACCCAAGAATGCCAGGAGAGCAAGGGCCTAAAGGAGAGAAAGGTGATCCTGGCGAGCCTGG AGCCCTGGGACCACAGGGCCATCCTGGAGAATTGGGTCCTCGGGGACCCATTGGACCTCCGGGTGCCAAG GGGCATGATGGTGCACAAGGACCTCCTGGAGCAGCTGGAAACCCTGGTGCTCCAGGACCTGCAGGACCCC CTGGTCTCAGTGGACCCCCTGGAAGTTTGGGTTCTCCTGGTGTCAGGGGTGCCCCTGGGAAAGATGGGGA GCGTGGAGAGAAGGGAACAGCAGGGGAAGAAGGCAGCCCAGGGCCAGCTGGTCCACGGGGAGATCCTGGT GCTCCTGGTCTCCCAGGGCCACCAGGAAAGGGCAAGGATGGGGAGCCGGGACTACGTGGACCACCTGGGT TACCTGGTCCCTTAGGAATCAAGGGTGACCGAGGAACACCTGGGATTCCTGGCTCTCCTGGCAGCCGTGG TGACCCAGGCATTGGGGTTGCTGGTCCACCTGGTCCCTCAGGACGACCAGGAGATAAAGGACCCCCGGGA TCTCGAGGCTTACCTGGATTCCCCGGCCCCCAGGGACCAGCTGGCCAGGATGGTGCACCAGGAAACCCAG GAGAAAGAGGGCCTCCTGGAAAACCAGGCCCTTCTTCACTGCTGTCTCCAGAGGACATAAATCTCTTAGT AAAGGATGTGTGCAACGACTGCCCTCCTGGTCCCCCAGGCCTCCCAGGTCTGCCAGGTTTCAAAGGGGAC AAGGGTCTTCCAGGAAAACAAGGGAGAGAGGGGACAGAAGGCAAAAAGGGAGATACCGGGCCTCCAGGCC CCCCTGGGCCTCCAGGAGTAGCTGGACCACAGGGAAGCCAAGGAGAACGTGGTGCAGAGGGTGAGGTGGG ACAGAAAGGTGAACAGGGTCACCCTGGAGTTCCAGGCTTCATGGGCCCACCGGGGAATCCTGGGCCACCA GGAGCAGATGGGAATGCAGGTGTGGCGGGGCCACCAGGACCTCAAGGGCCTCAGGGCAAAGAAGGCCCTC CTGGTCCCCAAGGTCCATCTGGCATCCCAGGAGTCCCAGGAGAAGAAGGCAAGCAGGGCAGAGATGGAAA GCCAGGTCCCCCTGGAGAACCGGGCAAGACAGGAGAGCCAGGCCTATCAGGAGCAGAGGGAGCCAGAGGT CCACCGGGCTTCAAGGGACACACAGGTGATCCTGGCCCACCTGGTCTGAGGGGAGAACCTGGCATAGCAG GACCCTCTGGAAGGGATGGATCACCAGGAAAAGATGGTGACACTGGACCTGCTGGACCACAGGGTCCCAG AGGAACAAGGGGACCACCGGGCAGCAGTGGATCACCTGGATCTCCAGGAGACCCTGGCCGTCCGGGAGCC CTAGGCCAGAAGGGAAACAAAGGAGAAAGTGGCAGCCCAGGACTTCCAGGGTTTCAGGGTCCTCGTGGAC CTCCAGGAGAAGCAGGAGAGGTAGGAGCTCCAGGAAAGGAGGGTGCTCCTGGGAAGCCTGGAGAGCCAGG ATCCAAAGGCGAGAGGGGAGACCCTGGGATCAAAGGTGACAAAGGACCACCAGGTGGGAAGGGTCAGCCT GGAGACCCTGGAACCCCAGGCCACAAGGGTCATACTGGCCTAATGGGTCCCCAAGGACAACCAGGAGAGA GTGGCCCTCCTGGGCCCCCAGGGCCACCTGGCCAGCCAGGATTTCCAGGACTGAGGGGGGAGTCTCCATC CATGGACACCTTACGGCGCCTCATTCAGGAAGAGCTTGGGAAGCAGCTAGAAGCTAAACTTGCCTACCTC CTAGCCCAGATGCCTCCAGCACACATGAAGTCCTCTCAAGGCAGACCCGGACCTCCTGGACCCCCTGGAA AAGATGGGCTGCCAGGTCGGACAGGCCCCATGGGTGAGCCAGGCCGTCCTGGCCAAGGTGGTCTGGAAGG ACCCTCTGGACCAATGGGCCCCAAAGGTGAAAGAGGAGCCAAAGGTGACCCTGGCACACCTGGAGTTGGT CTCCGAGGAGAGATGGGTCCTCCTGGAATACCAGGCCAACCTGGGGAACCTGGATACGCTAAAGATGGAC TTCCAGGAAGCCCTGGTCCTCAAGGAGAGACAGGACTAGCTGGACATCCTGGCCCTCCTGGTCCTCCTGG CCCACCTGGCCTGTGTGACCCCTCCCAGTGTGCGTACTTTGCCAGCCTTGCTGCCCGGCCAAGTAATGTG AAGGGCCCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_027174
Insert Size	4842 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_027174.1, NP_081450.1
RefSeq Size	6128 bp
RefSeq ORF	4842 bp
Locus ID	69700

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