Plch2 (NM_001113360) Mouse Untagged Clone
CAT＃: MC224609
Plch2 (untagged) - Mouse phospholipase C, eta 2 (Plch2), transcript variant 2, (10ug)
CNY 19190.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A930027K05Rik; Plc-eta2; PLCeta2; Plcl4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224609 representing NM_001113360
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGGACTGGCCTGGGGACCATCCAGGGCCGCAGGCAGCAGTTGGGTCAATGCCAGTGGGACCTGGG AACAGCCCTTGCGGGGCTTCAGTGGGCTCCAAGGAGGAAGACGAAGAGGTAGAGGAGAGAAGGGGATCCC TGAGGAGCCCCTGTGCCAGCTGACACCTCAGCTTGGCCTGTCGCTGAGGGTCCCTTTTGGCTTGGGGGAC TACGGCTTGGATATGCCTGGTCCCCAGCCGTCTGCTGCCAGCCAGACCACAGGAGCCGTGGCTTGCCTGG CAGAGGTACTCCTCTGGGTTGGAGGGAGCGTGGTAGTGTCACCAAGATGGCAGCTCAGCCTTGTAGTGGA GCGATGCATGAGTGCCATGCAAGAGGGGACCCAGATGGTGAAGCTCCGTGGCAGCTCCAAGGGATTGGTC CGCTTCTACTACCTGGATGAGCACCGCTCCTGCCTCCGATGGCGTCCCTCCCGCAAGAACGAGAAAGCCA AGATTTCCATCGACTCCATCCAGGAGGTGAGTGAGGGCCGGCAGTCTGAAATCTTCCAGCGCTACCCGGA CAGCAGCTTTGACCCCAACTGCTGCTTCAGCATCTACCATGGCAGCCACAGAGAGTCGCTGGACTTGGTC TCACCCAGCAGTGAAGAGGCACGTACCTGGGTCACCGGCCTTCGCTACCTCATGGCTGGCATCAGCGATG AAGACAGCCTAGCCCGTCGCCAGCGTACCAGGGACCAGTGGCTGAAGCAGACGTTTGATGAGGCTGACAA GAACGGGGACGGCAGCCTGAGCATCAGTGAGGTTCTGCAGCTGCTACATAAACTCAACGTGAACCTGCCC CGGCAGAGGGTGAAACAGATGTTCAGGGAGGCGGACACAGATGACCATCAGGGGACATTAGGCTTTGAGG AATTCTGCGCCTTCTACAAGATGATGTCTACCCGCCGAGACCTCTACCTGCTCATGCTGACCTACAGCAA CCATAAGGACCACTTGGATGCCTCCGACCTGCAGCGCTTTCTGGAGGTGGAGCAGAAGATGAACGGTGTG ACCCTGGAGAGCTGTCAGAACATCATTGAGCAGTTTGAGCCTTGCCTGGAAAATAAGAGCAAGGGGATGC TGGGGATTGATGGCTTTACCAACTACACCCGGAGCCCCGCCGGTGACATCTTCAACCCTGAGCACAACAG AGTGCACCAAGACATGACGCAGCCGCTGAGTCACTACTTCATCACCTCGTCCCACAACACCTACCTCGTG GGTGACCAGCTCATGTCCCAGTCTCGGGTGGACATGTATGCCTGGGTCCTGCAGGCCGGCTGCCGCTGTG TGGAGGTGGACTGCTGGGACGGGCCCGATGGAGAACCCATTGTCCATCATGGCTACACTCTGACCTCCAA GATCCTTTTCAAAGATGTCATCGAAACCATCAACAAATACGCCTTCATCAAGAATGAGTACCCAGTGATC CTGTCCATTGAGAACCACTGTAGTGTTGTTCAGCAGAAGAAGATGGCCCAGTATCTGACTGACATCCTCG GGGACAAGCTGGACCTCTCCTCAGTGAGCAGCGAAGATGCCACCATGCTTCCATCTCCACAGATGCTCAA GGGCAAGATCCTGGTGAAGGGGAAGAAGCTCCCTGCCAATATCAGTGAGGATGCAGAAGAAGGCGAAGTA TCTGATGAGGACAGTGCTGATGAGATGGAGGATGACTGCAAGCTCCTCAATGGGGATGCCTCCACCAATC GGAAGCGTGTGGAAAACATTGCAAAGAAGAAGCTGGATTCTCTGATCAAGGAGTCAAAGATCCGTGACTG TGAAGACCCCAATGACTTCTCTGTGTCCACACTATCCCCCTCTGGAAAGCTTGGGCGCAAAGCAGAGGCC AAGAAGGGTCAGAGCAAGGTTGAGGAAGACGTGGAGGCCGGGGAAGACAGCGGGGTGAGCAGGCAGAATA GCCGCCTCTTCATGAGCAGCTTCTCCAAGCGCAAGAAAAAAGGCAGCAAGATAAAGAAGGTGGCCAGCGT GGAAGAGGGGGACGAGACCCTGGACTCCCCAGGAAGCCAGAGCCGAGGGACTGCCCGGCAAAAGAAGACC ATGAAGCTGTCACGAGCCCTCTCGGACCTAGTGAAATATACCAAGTCTGTGGGGACCCATGACGTGGAGA TAGAGGTGGTATCTAGCTGGCAGGTGTCGTCCTTCAGTGAGACCAAGGCCCATCAGATCCTGCAGCAGAA GCCCACCCAGTACCTGCGCTTCAACCAGCACCAGCTCTCGCGCATATACCCCTCCTCCTACCGTGTGGAC TCCAGCAACTACAATCCACAACCCTTCTGGAACGCTGGTTGCCAGATGGTTGCCCTGAACTACCAGTCAG AGGGGCGGATGCTACAGCTGAACAGGGCCAAGTTCAGCGCCAACGGTGACTGTGGCTACGTGCTCAAACC CCAGTGCATGTGCCAGGGTGTCTTCAACCCCAACTCGGAGGATCCCCTGCCGGGGCAGCTCAAGAAGCAG CTGGCCCTGAGGATCATCAGTGGCCAGCAGCTGCCCAAACCACGGGACTCGGTGCTGGGCGACCGTGGGG AGATCATCGACCCCTTCGTGGAGGTGGAGGTCATTGGGCTCCCCGTGGACTGCAGCAAGGAGCAGACCCG AGTGGTGGACGACAACGGATTCAACCCCATGTGGGAGGAGACACTGGTGTTCACCGTGCACATGCCAGAG ATTGCGCTTGTACGCTTCCTGGTCTGGGACCATGACCCCATTGGACGTGACTTCATCGGCCAGAGGACAC TAGCCTTCAGCAGCATAATGCCAGGCTACCGGCATGTGTACCTAGAAGGGATGGAAGAGGCCTCTATCTT TGTTCATGTGGCTGTCAGTGACATTAGTGGTAAGGTCAAGCAGACTCTGGGTCTAAAAGGTCTCTTCCTC CGAGGCACAAAGCCAGGCTCGCTGGACAGTCATGCTGCTGGACAGCCCCTCCCCCGGCCCTCCGTTAGCC AGAGGCTCCTGCGGCGCACGGCCAGTGCCCCGACCAAAAGCCAGAAGCCAAGTCGCAAGGGCTTCCCAGA GCTGGCCCTGGGCACACAGGACGCAGGCTCCGAGGGGGCAGCTGATGACGTGGCACCCTCTAGCCCCAAC CCAGCTCTGGAGGCCCCTACTCAAGAGAGGTCAGGCAGCAGCAGCCCCCGAGATACTCGCCTCTTCCCCC TGCAGCGGCCCATCTCCCCCCTGTGCAGCCTGGAACCCATTGCCGAGGAACCAGCCCTGGGCCCTGGCCT CCCGTTACAGGCAGCTGCCCCCACAGGTCCATCTCAGGAAGGGTCCCAGTGCCCTGTAGGCCTGGGAGCC AAAGTGACCAGCTCCCAGCAGACATCTCTGGGAGCTTTTGGAACCCTCCAGCTCAGGATTGGGGGCGGTA GGGAAAACGAAGAGCCACCCCTCAGGCCACACAATGGTGGGATCTCCAGCGGGCCACGTGAGGGGACTTC TGGAAGACAGACAGACAGCAAGAGCCGCTCCCGGGTCCCGGGCCACTTGCCAGTGGTTAGGAGGGCCAAG AGTGAGGGCCAGGTGCTCTCGGAGCTCTCTCCCACACCTGCAGTGTACTCGGATGCCACGGGCACTGACA GGTTATGGCAGCGGCTGGAGCCTGGGAGCCACAGGGACAGTGTCTCCTCATCCTCCAGCATGTCCTCCAA CGACACCGTCATAGACCTATCACTGCCCAGCCTCGGCCTCTGCCGCAGCCGAGAGAGCATTCCAGGGGTC TCTCTTGGACGCCTAACCTCACGGCCCTGCTTAGCCTCAGCTGCCCGTCCTGACCTGCCTCCTGTGACCA AGAGCAAATCGAACCCCAACCTGCGGGTTGCGGGTGGGCTGCCTACTGCACCCGACGAGCTGCAGCCCCG GCCTCTTGCCCCGCGGCTGACCGGCCACCATCCACGGCCACCGTGGCATCACCTCACCCTGGTGGGCCTG CGGGACTGCCCAGTGTCTGCTAAGTCCAAGAGTCTGGGAGACCTGACAGCTGACGACTTTGCTCCCAGCT TTCAGGGCAGCACCAGCAGCCTGAGCTGCGGCCTAGGCTCCCTGGGTGTGGCGCATCAGGTGCTGGAGCC GGGAATACGGCGAGATGCTCTCACGGAGCAGCTACGCTGGCTCACCGGCTTCCAGCAGGCTGGGGACATC ACGTCGCCCACCAGCTTGGGCCCAGCTGGGGACGGGTCTGTAGGGGGCCCCAGCTTCCTGAGGCGTTCCT CCTCTCGGAGCCAGAGCCGCGTGCGCGCTATTGCCAGCCGAGCCCGCCAAGCGCAGGAGCGCCAGCAGAG GCTGAGAGGCCAGGACTCACGGGGTCCCCCAGAGGAGGAGCGGGGCACCCCTGAGGGTGCCTGTTCTGTA GGCCATGAGGGCTGTGTGGATGTGCCAATGCCTGCCAAGGGAGCCCCTGAGCAGGTGTGTGGTGCTGCTG ATGGCCAGCTGCTGCTCAGGCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001113360 |
| Insert Size | 4506 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001113360.2, NP_001106831.1 |
| RefSeq Size | 5067 bp |
| RefSeq ORF | 4506 bp |
| Locus ID | 269615 |
| UniProt ID | A2AP18 |
| Gene Summary | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This phospholipase activity is very sensitive to calcium. May be important for formation and maintenance of the neuronal network in the postnatal brain.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) encodes the longest isoform (b). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222577 | Plch2 (tGFP-tagged) - Mouse phospholipase C eta 2 (Plch2) transcript variant 2, (10ug) |
CNY 14440.00 |
|
| MR222577 | Plch2 (Myc-DDK-tagged) - Mouse phospholipase C, eta 2 (Plch2), transcript variant 2 |
CNY 11296.00 |
|
| MR222577L3 | Lenti ORF clone of Plch2 (Myc-DDK-tagged) - Mouse phospholipase C, eta 2 (Plch2), transcript variant 2 |
CNY 15010.00 |
|
| MR222577L4 | Lenti ORF clone of Plch2 (mGFP-tagged) - Mouse phospholipase C, eta 2 (Plch2), transcript variant 2 |
CNY 15010.00 |
