Sipa1l1 (NM_001167983) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4931426N11Rik; AW213287; mKIAA0440; Spar
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224597 representing NM_001167983 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAGTTTGAAGCGGTCGCAGACCGAAAGACCTGTCACCGCTGACAGAGCCTCTGTTGTCAGCACAG ATGGCGCCCCCAAAGTCCACACCGATGACTTCTACATGCGTCGCTTCCGCTCTCAGAATGGCAGCCTAGG ATCATCAGTCATGGCTGCAGTGGGGCCCCCTCGAAGTGAAGGCCCTCACCATATCACCTCAACCCCCGGG GTCCCCAAGATGGGGGTTAGGGCAAGAATAGCAGATTGGCCTCCGAGAAAGGAAAATGTAAAAGAATCCA GCCGTTCAAGCCAGGAAATAGAAACCTCAAGTTGCCTTGAGAGCCTGTCCTCCAAAGGCAGTCCTGTGAG TCAGGGGAGTTCTGTTAGCCTCAATTCCAATGACTCAGCCATGCTGAAGAGCATACAGAACACCCTGAAG AACAAGACAGGGCCAGCGGAGAGCATGGACTCCAGATTCCTCATGCCTGAAGCCTACCCCAGTTCCCCCA GGAAAGCCCTTCGCAGAATTCGGCAGCGCAGCAACAGTGATATCACCATAAGTGAGCTTGATGTGGATAG CTTCGATGAATGTATCTCCCCAACCTACAAGTCGGGGCCATCATTGCACAGGGAATATGGCAGCACATCT TCAATCGACAAGCAGGGAACATCCGGAGACAGCTTCTTCGATTTGTTAAAGGGCTATAAAGATGACAGAT CTGACCGAGGTCCAACTCCAACCAAACTCAGTGACTTCCTCATCACTGGTGGGGGCAAGGGTTCTGGTTT CTCCTTGGATGTGATCGATGGCCCCATCTCACAGAGAGAGAACCTCAGGCTTTTCAAGGAAAGGGAAAAA CCACTCAAGCGACGCTCTAAGTCTGAGACTGGAGACTCGTCCATTTTTCGTAAATTGCGCAATGCCAAAG GTGAAGAACTCGGGAAATCATCAGACCTTGAAGACAACAGATCAGAAGATTCTGTGAGGCCCTGGACATG TCCAAAGTGCTTTGCCCACTATGATGTCCAGAGCATATTGTTTGACTTGAATGAAGCCATTATGAACAGA CATAATGTGATTAAGAGGAGAAACACCACAACAGGAGCTTCGGCGGCTGCGGTGGCATCCTTGGTCTCCG GACCTCTGTCTCACTCAGCCAGCTTCAGCTCTCCCATGGGCAGCACAGAGGACCTCAACTCCAAAGGAAG CCTTGGCATGGACCAGGGAGATGACAAGAGCAATGAACTCGTCATGAGCTGTCCGTATTTTCGGAATGAG ATTGGGGGAGAAGGTGAGAGGAAGATCAGCCTGTCCAAGTCGAATTCTGGCTCATTTAGTGGGTGTGAGA GCACATCCTTTGAGTCTGCCCTCAGCTCTCACTGCACCAACGCGGGCGTGGCAGTTCTCGAAGTGCCCAA GGAAAGCTTGATGCTGCATCTGGACAGGGTGAAAAGGTACACCGTGGAACACGTGGATCTTGGCGCATAC TATTACAGGAAGTTCTTCTACCAGAAGGAACACTGGAACTATTTTGGGGCTGATGAGAACCTCGGTCCAG TGGCTGTGAGCATTCGAAGGGAAAAACCAGAAGACATGAAGGAAAACGGATCTCCATACAACTACCGAAT AATATTCAGGACTAGTGAGCTCATGACGCTGAGGGGGTCTGTCCTGGAGGATGCCATTCCCTCCACGGCC AAGCACTCGACAGCCAGGGGATTGCCTCTGAAAGAGGTGCTGGAACACGTGATCCCAGAGCTCAACGTGC AGTGCCTGCGCTTGGCCTTCAACACACCCAAAGTCACAGAGCAGCTCATGAAACTGGACGAGCAAGGGCT GAACTATCAGCAGAAAGTAGGCATCATGTACTGCAAAGCAGGCCAGAGCACGGAGGAGGAGATGTACAAC AACGAGTCTGCAGGCCCAGCCTTTGAGGAGTTCCTTCAGCTGCTGGGGGAACGAGTCCGGCTAAAAGGAT TCGAGAAGTATCGTGCGCAGCTTGACACCAAAACTGACTCCACTGGAACCCACTCTCTGTACACAACCTA CAAAGACTATGAGATAATGTTCCACGTCTCCACCATGCTGCCCTACACACCTAACAACAAGCAACAGCTC CTGAGGAAGCGGCACATTGGGAATGACATTGTGACAATAGTTTTCCAAGAGCCTGGAGCACAACCATTCA GCCCGAAAAACATCCGGTCTCACTTTCAGCATGTTTTTGTCATTGTCCGGGCTCACAACCCTTGCACTGA GAGTGTCTGTTACAGTGTGGCAGTCACCAGGTCCAGAGATGTACCTTCTTTTGGACCTCCCATCCCTAAA GGGGTCACCTTCCCCAAGTCAAATGTGTTCAGGGACTTCCTTTTGGCCAAAGTGATAAATGCAGAAAATG CTGCTCATAAATCAGAGAAGTTCCGGGCCATGGCGACAAGGACCCGCCAGGAATACCTGAAAGATCTGGC AGAAAAGAATGTCACCAACACACCTATTGACCCTTCTGGCAAGTTTCCATTTATTTCTCTGGCCTCCAAG AAGAAGGAAAAGTCTAAGCCTTATCCAGGAGCTGAGCTCAGTAGCATGGGGGCCATTGTGTGGGCTGTCC GGGCCAAAGACTACAACAAGGCCATGGAGTTCGACTGCCTCCTTGGGATCTCCAGCGAGTTCATCGTCCT CATTGAGCAGGAGACAAAGAGTGTGGCTTTCAATTGCTCCTGCAGAGATGTCATAGGGTGGACTTCCAGC GACACCAGCCTCAAAATCTTCTATGAGCGGGGAGAATGTGTGTCGGTGGAGAGCTTCATTAGCGGTGAAG ATATCAAAGAAATTGTCAGAAGGCTGCAGTTTGTTTCAAAAGGTTGTGAATCTGTGGAAATGACTCTGCG AAGAAATGGGCTGGGGCAGCTTGGCTTCCATGTCAACTATGAGGGCATTGTGGCGGATGTAGAACCCTAC GGCTACGCATGGCAAGCAGGGCTGAGGCAGGGCAGCCGCCTGGTGGAGATCTGCAAGGTAGCAGTGGCCA CCCTGAGCCATGAACAGATGATCGATCTCTTGAGAACATCAGTCACAGTGAAGGTTGTCATTATCCCTCC CCATGACGACTGCACCCCACGGAGGAGTTGCTCAGAAACCTACCGCATGCCAGTGATGGAGTACCAGATG AATGAAGGCATTTCCTACGAGTTCAAGTTTCCCTTCCGGAATAATAACAAATGGCAGCGGAATGCCAGCA AGGGTGCTCATTCGCCCCAGGTTCCATCTCAGCTGCAGAGTCCCATGACCTCACGACTGAATGCTGGGAA GGGAGATGGGAAAATGCCCCCTCCAGAAAGAGCTGCCAACATCCCTCGAAGCATCTCCAGTGACGGGCGC CCACTGGAAAGGAGGCTGTCTCCTGGTTCGGACATCTATGTGACAGTCTCATCCATGGCTTTGGCGAGAT CCCAGTGCCGTAACTCTCCCAGCAACTTGTCTTCGTCCAGTGAGACTGGCTCTGGAGGTGGTACCTACAG ACAAAAATCCATGCCCGAAGGGTTTGGGGTGAGCCGAAGATCCCCAGCTTCCATCGACAGGCAGAACACC CAGTCGGATATAAGTGGCAGTGGAAAATCCACTCCCAGCTGGCAGAGAAGTGAGGACAGCCTTGCCGACC AGATGGAGCCGACGTGCCATCTCCCAGCAGTATCGAAGGTACTGCCTGCTTTCCGAGAGAGCCCCAGTGG GAGATTGATGCGGCAGGATCCAGTGGTTCACTTGTCTCCAAACAAACAAGGCCATTCTGACAGCCACTAC TCCAGCCACTCCAGCAGCAACACGCTCTCCAGCAACGCCTCGAGTGCACACAGTGACGAGAAGTGGTACG ATGGGGACCGCACGGAGTCCGACCTCAACAGCTACAACTACCTACAGGGCACGTCTGCCGACAGCGGGAT TGACACCGCCTCCTACGGCCCCAGCCATGGCAGCACGGCCTCCCTGGGGGCCTCCACATCCTCACCTCGT TCAGGGCCAGGCAAAGAAAAGGTGGCTCCCCTGTGGCACAGCTCCAGTGAAGTGCTCTCCCTGGCAGATC GGACCTTAGAGACTGAGGGCCACGGCATGGACAGGAAAGCAGAGTCCTCCCTGAGCCTGGACATCCACAG CAAGAGCCAGGGCGGGTCAAGCCCGCTGAGCAGGGAGAACAGCACCTTCAGCATAAATGATGCTGCGTCC CACACCAGTACCATGAGCTCCCGACACTCTGCCAGCCCAGTGGTATTCTCCAGTGCCAGAAGTTCCCCCA AAGAGGAGCTTCACCCCACCGCATCCTCCCAGCTCGCACCGTCCTTTTCCTCTTCTTCCTCATCCTCCTC TGGACCTAGGACTTTCTACCCTCGCCAGGGCGCCACTAGCAAATATCTGATTGGATGGAAAAAGCCAGAA GGAACCATTAACTCCGTGGGATTTATGGACACACGAAAGCGACATCAGAGTGATGGCAATGAGATAGCCC ACACTAGGCTTCGAGCCTCAACCAGGGACCTGCAGGCATCCCCAAAGCCGACCTCCAAGTCTACCATTGA GGAAGATCTAAAGAAACTCATCGACCTTGAGAGCCCAACTCCCGAATCCCAGAAGAATTTCAAGTTCCAT GCACTGTCCTCCCCGCAGTCCCCGTTCCCCACTACCCCTACCTCCCGGCGGGCCCTGCACAGGACTCTGT CAGATGAGAGCATTTACAGCAGCCAGAGGGAGCATTTCTTCACCTCCAGGGCTTCGCTTCTAGACCAAGC CCTGCCCAACGATGTCCTCTTCAGCAGTACCTACCCATCTCTCCCCAAGTCACTTCCACTGAGGAGGCCA TCTTACACGTTGGGAATGAAGTCATTGCATGGAGAGTTCTCTGCCTCGGACAGCTCCCTCACCGACATCC AGGAGACCCGAAGGCAGCCTATCCCTGACCCTGGCCTGATGCCCCTGCCTGATGCAGCTTCAGATTTGGA CTGGTCCAACCTAGTAGATGCCGCCAAAGCCTATGAGGTCCAGAGAGCCTCATTTTTTGCTGCTAGTGAT GAAAACCATCGCCCCCTGAGCGCGGCCTCCAACAGTGACCAGCTGGAGGAGCAGGCCCTGGTCCAGATGA AGTCCTACAGCAGTAAGGACCCCTCTCCCACTCTGGCTTCTAAGGTGGACCAGCTGGAAGGTATGCTGAA AATGCTTCGAGAAGATTTGAAGAAGGTAAATGCTCTCAGAAAAAGAAGACAAGGCCCAGCTGCAGGCGGA AGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001167983
Insert Size	5256 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001167983.1, NP_001161455.1
RefSeq Size	7717 bp
RefSeq ORF	5256 bp
Locus ID	217692
UniProt ID	Q8C0T5
Gene Summary	Stimulates the GTPase activity of RAP2A. Promotes reorganization of the actin cytoskeleton and recruits DLG4 to F-actin. Contributes to the regulation of dendritic spine morphogenesis (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate donor splice site at the penultimate coding exon compared to variant 1, resulting in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1.

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