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Rapgef2 (NM_001099624) Mouse Untagged Clone
CAT＃: MC224556
Rapgef2 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), (10ug)
CNY 19,100.00
货期*
9周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5830453M24Rik; CNRasGEF; mKIAA0313; nRapGEP; Pdzgef1; RA-GEF-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224556 representing NM_001099624
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTCCTTGTGCGTTGCTATGCCAACTGCCTGCAGCCATGGTCCTCCAAACTCCCTGCAGACTTCA CAAAACTGCACCTCACTGACAGCCTGCACCCACAGGTGACCCACGTGTCCTCCAGCCATTCAGGATGTAG CATCACCAGCGACTCTGGGAGCAGCAGTCTTTCTGACATTTACCAGGCCACGGAGAGCGAGGCCGGTGAC ATGGACCTGAGTGGACTGCCAGAGACGGCGGTTGACTCTGAGGATGACGATGATGAGGAAGACATCGAGA GAGCATCAGACCCTCTGATGAGCAGGGACATCGTGAGGGACTGCCTGGAGAAGGACCCGATAGACAGGAC TGACGATGACATCGAACAACTACTGGAATTTATGCACCAGCTGCCGGCCTTTGCCAATATGACGATGTCA GTAAGACGGGAGCTCTGTGCCGTGATGGTGTTTGCAGTGGTGGAAAGAGCTGGAACCATTGTGTTAAACG ACGGAGAAGAGCTGGATTCCTGGTCAGTGATTCTCAATGGTTCCGTGGAGGTGACATATCCGGATGGGAA AGCAGAAATTCTGTGCATGGGAAACAGCTTTGGTGTTTCGCCCACCATGGACAAAGAATACATGAAAGGA GTCATGAGGACTAAGGTGGATGACTGCCAGTTTGTCTGCATTGCCCAGCAAGATTACTGCCGTATTTTGA ACCAAGTAGAAAAGAACATGCAAAAAGTTGAGGAGGAAGGAGAGATTGTAATGGTGAAGGAGCACCGAGA ACTTGATCGGACTGGAACAAGGAAGGGCCACATCGTCATCAAGGGCACCTCAGAGAGGCTGACCATGCAT TTGGTGGAAGAGCATTCTGTAGTGGATCCAACATTCATAGAAGACTTCCTGTTGACATACCGGACTTTCC TTTCCAGCCCGATGGAAGTGGGCAAGAAGTTATTGGAGTGGTTCAATGACCCGAGCCTCAGGGATAAGGT GACGCGGGTAGTATTACTGTGGGTGAATAATCACTTCAATGACTTTGAAGGGGATCCTGCAATGACTCGA TTTCTGGAAGAATTTGAAAATAATCTGGAAAGAGAGAAAATGGGTGGACATCTAAGGCTGTTGAACATTG CATGTGCAGCTAAAGCCAAACGAAGACTGATGACGTTAACAAAGCCTTCCCGGGAAGCCCCTCTGCCCTT CATCTTACTGGGAGGCTCTGAAAAGGGCTTTGGCATCTTCGTGGACAGCGTTGACTCGTGTAGCAAAGCC ACTGAAGCAGGCTTGAAGCGCGGGGATCAGATATTAGAAGTGAATGGTCAAAATTTTGAAAATATCCAAC TTTCAAAGGCAATGGAAATTCTCAGAAATAACACACACCTGTCCATCACTGTGAAAACCAACTTATTTGT ATTTAAAGAACTTCTGACGAGATTGTCTGAAGAGAAAAGAAACGGTGCCCCTCACCTCCCTAAAATCGGG GACATCAAGAAGGCCAGCCGCTACTCCATACCGGATCTCGCCGTAGACGTAGAACAGGTGATAGGCCTCG AGAAGGTCAACAAGAAAAGCAAAGCCAACACCGTGGGAGGAAGAAACAAGCTGAAGAAAATACTCGACAA GACCCGGATCAGCATCTTACCTCAGAAACCATACAATGATATTGGGATTGGTCAGTCTCAAGATGATAGC ATCGTAGGACTGAGGCAGACCAAGCACATCCCAGCTGCCCTGCCTGTCAGTGGAACGCTGTCCTCCAGTA ACCCTGACTTGCTGCAGTCACACCACCGGATTCTGGACTTCAGCACTACTCCTGACTTGCCAGATCAGGT ACTGAGAGTTTTCAAGGCTGACCAACAAAGCAGGTACATCATGATCAGCAAGGACACCACCGCAAAGGAA GTGGTCATCCAGGCCATCAGAGAGTTTGCTGTCACTGCCACCCCAGAGCAGTATTCCCTCTGTGAGGTCT CAGTCACACCAGAGGGAGTTATCAAACAACGCCGACTCCCAGATCAGCTTTCCAAGCTTGCCGACAGGAT ACAACTGAGTGGAAGGTATTATCTGAAAAACAACATGGAAACAGAAACGCTTTGCTCAGATGAAGATGCC CAGGAGCTGCTGCGAGAGAGCCAAATCTCCCTCCTTCAGCTCAGCACCGTGGAGGTTGCCACGCAGCTCT CCATGAGGAACTTTGAACTCTTCCGCAACATTGAGCCTACTGAATACATAGATGATTTATTTAAACTCAA ATCAAAAACCAGCTGTGCCAACCTGAAGAAATTTGAGGAAGTCATTAACCAGGAAACATTTTGGGTGGCT TCTGAAATCCTGCGGGAAACAAACCAGCTGAAGAGGATGAAGATCATTAAGCATTTCATCAAGATTGCCT TGCACTGCAGGGAATGCAAGAATTTTAACTCCATGTTTGCAATCATCAGTGGCCTAAACCTGGCACCGGT GGCAAGACTACGAACCACCTGGGAAAAGCTTCCCAATAAATATGAAAAGCTGTTCCAGGACCTGCAAGAC CTGTTTGATCCTTCCAGAAACATGGCAAAGTACCGCAATGTCCTGAGTGGCCAGAACTTACAGCCTCCTG TCATTCCCCTGTTCCCTGTCATCAAGAAGGACCTCACGTTTCTCCATGAGGGAAATGACTCAAAAGTAGA CGGACTGGTCAACTTTGAGAAGCTGAGGATGATTGCAAAAGAAATCCGTCACGTTGGCCGAATGGCATCA GTCAACATGGACCCTGCCCTTATGTTCAGGACTCGGAAGAAGAAGTGGCGGAGTCTGGGGTCCCTGAGTC AGGGCAGTGCAAACGCCACAGTGCTGGATGTTGCTCAGACAGGCGGGCATAAAAAGCGGGTACGCCGTAG TTCCTTCCTCAATGCCAAAAAGCTGTATGAAGATGCCCAGATGGCTCGGAAAGTGAAGCAATACCTGTCC AACCTGGAGCTGGAGATGGATGAGGAGAGTCTTCAGACACTGTCACTGCAGTGTGAACCAGCAACCAGCA CATTGCCCAAGAATCCTGGAGACAAAAAGCCTGTCAAGTCTGAGACCTCCCCAGTGGCTCCACGTGCAGG GCCCCAGCAGAAAGTACAGCCTCAGCAGCCGCTGGCACAGCCACAACCTCCACATAAAGTCAGCCAGGGA CTGCAGGTGCCCGCTGTGTCCCTCTATCCTTCACGAAAGAAGGTGCCGGTGAAGGATCTGCCTCCTTTTG GCATAAACTCTCCACAAGCTTTGAAGAAAATCCTGTCCTTGTCTGAAGAAGGGAGTTTGGAGCGTCACAG GAAGCAAGCAGAAGATACAATATCAAATGCGTCCTCTCAGCTCTCATCGCCCCCCACCTCACCTCAGAGC TCTCCAAGGAAAGGTTATGCACTGGCTCTCAGTGGCACCGTGGATAATTTCTCAGATTCAGGTCACAGTG AGATTTCTTCACGATCCAGTATTGTCAGCAATTCTTCTTTTGACTCGGTGCCAGTCTCTCTGCATGATGA ACGGCGCCAGAGGCATTCTGTCAGCATTGTGGAGAGCAACCTAGGTGTGGGCAGGATGGAGCGGCGGACC CTGATGGAGCCTGATCAATACAGCTTAGGGTCGTATGCCCCAGTGTCTGAAAGCCGAGGCCTGTATGCCG CAGCGACGGTCATCTCTTCTCCTAGCACAGAGGAGCTTTCCCATGACCAAGGGGACCGTGCTTCCCTCGA TGCTGCTGACAGCGGTCGTGGGAGCTGGACATCATGCTCAAGTGGTTCCCATGACAACATACAGACCATT CAGCACCAGAGAAGCTGGGAAACACTGCCATTTGGGCACACTCACTTTGACTATTCAGGGGATGCTGCAA GTATATGGGCCTCCGGTGGCCATATGGACCAAATGATGTTTTCTGATCATAGCACAAAATATAACAGACA GAATCAAAGTAGAGAGAGTCTTGAACAAGCCCAATCCCGGGCCAGCTGGGCGTCTTCCACGGGCTACTGG GGAGAAGACTCAGAAGGCGACACAGGCACTATCAAGCGAAGGGGTGGGAAGGATGTCTCCGCTGAGGCAG AGAGCAGCAGCATGGTGCCCGTGACTACAGAGGAAGCCAAACCTGTCCCTATGCCTGCCCACATAGCTGT GACGCCGAGCACTACCAAGGGACTCATCGCACGGAAGGAAGGCAGGTACCGGGAGCCGCCTCCCACACCT CCAGGCTACGTGGGCATCCCCATTGCCGATTTCCCAGAAGGGCCTTGCCACCCGGCCAGGAAGCCCCCGG ATTACAACGTGGCCCTGCAGCGGTCCCGCATGGTGGCACGGCCCACTGAGGCCCCGGCACCGGGCCAGAC GCCGCCTGCAGCCGCAGCCAGCCGGCCGGGCAGCAAGCCACAGTGGCACAAGCCCAGCGACGCAGACCCA CGCCTCGCGCCCTTCCAGCCGCAGGGCTTCGCAGGAGCGGAGGAGGACGAAGATGAACAAGTGTCTGCTG TTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001099624 |
| Insert Size | 4485 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001099624.3, NP_001093094.2 |
| RefSeq Size | 6583 bp |
| RefSeq ORF | 4485 bp |
| Locus ID | 76089 |
| Gene Summary | Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP or not. Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG219556 | Rapgef2 (tGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), (10ug) |
CNY 14,350.00 |
|
| MR219556 | Rapgef2 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2) |
CNY 10,224.00 |
|
| MR219556L3 | Lenti ORF clone of Rapgef2 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2) |
CNY 14,920.00 |
|
| MR219556L4 | Lenti ORF clone of Rapgef2 (mGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2) |
CNY 14,920.00 |
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