Dhx29 (NM_172594) Mouse Untagged Clone
CAT＃: MC224339
Dhx29 (untagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 29 (Dhx29), (10ug)
CNY 14016.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 3732415M03; AU043276; Ddxx; E130202M19Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224339 representing NM_172594
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGGCAAAAATAAGAAACACAAGGCGCCGGGAGCTGCGGCGATGCGGGCCGCCGTGTCTGCTTCCA GAGCCAGAAGTGCAGAGGCCGGAGCTGTTGGGGAAGCCCAGAGCAAGAAGCCGGTGGCCAGGCCGGCGCC CGCCGTCCCCACCGGCGCCCGGGAGCCCCGAGTGAAGCAAGGTCCAAAAATTTATAGTTTTAATTCTGCA AATGATTCCGGTGGCTCTGCAAATCTGGACAAATCTATTTTGAAAGTGGTGATTAACAATAAACTAGAGC AAAGGATTATCGGAGTGATCAATGAGCATAAAAAGCAGAACAGCGACAGGGGAGCGATCTCTGGAAGACT GTCGGCTAAGAAGCTACAGGATTTATACATGGCTTTACAAGCGTTTTCCTTTAAGACTAAGGATATTGAA GATGCCATGACCAATACACTCTTGCATGGAGGTGACCTTCATTCTGCCTTGGACTGGCTCTGCTTAAATC TTTCCGATGATGCACTTCCTGAAGGATTCAGTCAAGAATTTGAAGAGCAGCAACCTAAAAGCAGGCCAAA ATTCCAGTCTGTACAAATACAGGCTACTCTTTCACCTCCACAGCAGACGAAAACCAAAAGGCAGGAGGAA GACCCTAAAATTAAGCCAAAAAAGGAAGAGACAACCGTGGAGGTGAATATGAAGGAGTGGATTTTGAGAT ATGCTGAGCAACAGGACGAAGAGGAGAAGGGCGAGGGCTCTAAAGGCTTAGAGGAAGAAGAAAAGTTTGA CCCCAACCAAAGGTACTTGAACCTTGCAGCCAGACTTCTGGACGCAAAGGAGCAAGCCGCCGCCTTCAAA CTAGAAAAAAACAAGCAAGGCCAAAAAGAGGCTCAAGAGAAAATAAGAAAGTTCCAAAGAGAAATGGAAA CTTTAGAAGACCATCCAATATTCAACCCAGCCATAAAGATTTCACATCAACAAAATGAGAAGAAAAAGCC TGCTCCAGCCACAGAAGCTGAGAGTGCTTTAAACCTGAATTTATTTGAAAAATCTGCAGCTGCTACCGAG GAAGAGAAAGGTAAAAAGAAAGAACCTCACGATGTAAGGAACTTTGACTACACTGCTCGAAGCTGGACTG GCAAATCTCCCAAACAATTCCTCATCGACTGGGTCAGGAAGAATCTTCCCAAGAGTCCAAACCCCTCCTT TGAGAAAGTTGCCGTAGGCAGATACTGGAAATGTAGGGTAAGGGTCGTCAGGTCTGAAGATGACGTGCTG GTTGTGTGTCCTACAATCTTAACCGAGGACGGCATGCAAGCCCAGCACCTGGGAGCCACATTAGCCCTTT ATCGGCTAGTGAAGGGGCAGTCTGTCCATCAGTTACTCCCTCCTACGTACCGGGATGTTTGGCTGGAGTG GAGTGATGAAGAGAAGAAAAGGGAAGAACTCAATAAAATGGAAACCAACAAACCACGGGATCTTTTTATT GCCAAGCTCTTGAATAAGTTGAAGCAGCAGCAGCAACAACAGCAGCAGCAGCGTCCTGAAAGCGAGAAAG GGGGCTCCGAGGATCCCGAGGAATCCTGGGAAAACTTAGTTTCCGACGAAGATCTTGCTGCGCTGTCCTT GGAGCCAACCAGTGCAGAAGACCTGGCACCTGTTAGGAGCCTCTTCAGGAGGCTACAGAGCACACCCAAG TATCAGAGGCTCCTGAAGGAGAGACAGCAGCTACCTGTGTTCAAACACCGGGACTCAATTGTGGAAACTC TGAAGAGGCACCGGGTGGTGGTGGTAGCAGGTGAAACGGGGAGTGGCAAGAGCACCCAGGTGCCACACTT CCTGTTGGAAGATCTGCTGCTCGATGAGTGTGGAGCCAGGAAGTGTAACATAGTCTGCACCCAGCCCCGA AGAATCTCGGCGGTGAGCTTAGCCACCAGGGTGTGCGAGGAGCTGGGCTGTGAGAGTGGCCCTGGAGGAA GGAATTCTTTGTGTGGCTATCAGATCCGGATGGAATCTCGAGCTAGTGAATCTACCAGGCTACTGTATTG TACAACCGGAGTTTTGCTAAGGAAACTTCAGGAAGATGGTCTTCTGGCTGATGTGTCTCATGTCATTGTC GATGAGGTCCATGAGAGAAGTGTCCAGTCAGATTTCCTGCTCGTTATCCTGAAGGAGATTCTGCAGAAGC GTTCTGACCTACACTTGATTCTGATGAGCGCCACCGTGGACAGTGACAAGTTCTCTACGTACTTCACACA CTGTCCTATTCTCAGAATTTCAGGAAGAAGTTACCCTGTTGAGGTTTTTCATCTTGAAGACATAGTAGAA GAAACAGGCTTCGTCCTGGAGAAAGACTCTGAGTATTGTCAGAAGTTTCTGGAAGAGGAGGAAGAAATTA CCATTAATGTTACAAGTAAAGCTGGGGGAGTCAAAAAGTATCAGGAATACATCCCAGTTCAGAGTGGAGC AAGTCCTGAATTAAACCCGTTCTACCAAAAGTACAGTAGCCGTACGCAGCATGCAATTCTCTACATGAAC CCCCATAAAATCAACCTGGACCTCATTTTGGAGCTTCTTGTATACTTAGACAAAAGTCCTCAGTTCAGAA ATATTGAAGGAGCAGTATTGATCTTCTTACCTGGCCTTGCTCACATTCAACAGTTGTATGACCTCCTGTC AAGTGACAGAAGGTTTTATTCTGAGCGGTATCAAGTTATAGCTCTGCATTCAGTTCTCTCAACTCAAGAT CAAGCGGCCGCCTTCATGTTCCCCCCTCCCGGGGTCAGGAAGATTGTCTTAGCAACAAACATTGCAGAGA CAGGGATAACTATTCCTGATGTTGTGTTTGTCATTGACACTGGACGGACAAAAGAAAATAAGTACCATGA AAGCAGCCAGATGAGCTCTTTGGTGGAGACCTTTGTCAGTAAAGCTAGTGCCCTACAGCGCCAGGGGAGA GCCGGGAGGGTCCGGGATGGCTTCTGCTTTCGGCTGTACACAAGAGAAAGATTTGAAGGTTTTCTGGACT ATTCTGTACCTGAGATCCTGCGCGTCCCTCTGGAGGAACTCTGTCTTCACATCATGAAGTGTGATCTGGG CTCCCCTGAAGACTTCCTCTCCAAAGCCTTAGACCCTCCTCAGCTTCAAGTCATCAGCAACGCGATGAAC TTACTCCGGAAAATTGGAGCCTGCGAACCCAATGAGCCTAAACTGACACCGCTGGGCCAGCACCTTGCCG CTTTGCCTGTCAATGTCAAAATTGGCAAGATGCTCATTTTCGGAGCCATATTTGGCTGTCTCGAACCAGT GGCAACACTGGCAGCCGTGATGACAGAGAAGTCTCCATTCATCACACCAATTGGTCGAAAGGATGAAGCG GACCTTGCAAAGTCGTCTTTGGCTGTGGCCGACTCGGACCACCTCACGATCTACAATGCTTATCTAGGGT GGAAGAAAGCCCAGCAAGAAGGAGGCTTCCGCTCTGAGATCTCATATTGCCAGAGGAACTTCCTAAACAG AACGTCACTGTTGACACTCGAGGATGTGAAGCAGGAGTTAATGAAATTGGTGAAAGCTGCAGGATTTTCA TCGTCTCCCAGCTGGGAGGGAAGGAAAGGGCCGCAGACGCTCTCTTTCCAGGACATTGCTCTTCTTAAAG CTGTGCTGGCTGCTGGCCTGTACGACAGTGTGGGGAAGATCATGTGTACAAAGTCCGTGGACGTGACAGA GAAGCTGGCGTGCATGGTGGAGACAGCCCAAGGCAAAGCTCAGGTGCACCCGTCCTCTGTAAACCGGGAC CTACAAACCTACGGGTGGCTCTTGTACCAGGAGAAGGTGAGGTACACCAGAGTCTACCTGAGAGAGACGA CCCTCATCACCCCTTTTCCAGTTTTGCTTTTTGGAGGTGACATAGAAGTCCAGCACCGAGAGCGGCTTCT GTCTGTGGACGGCTGGATCTATTTTCAGGCCCCGGTAAAGATAGCTGTCATTTTTAAGCAGCTAAGAGTT CTCATTGATTCTGTTTTAAGAAAAAAACTTGAAAATCCAAAGATGTCCCTTGAAAATGACAAGATTCTAC AGATCATTACAGAATTGATAAAAACAGAGAATAATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_172594 |
| Insert Size | 4098 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057112, AAH57112 |
| RefSeq Size | 4098 bp |
| RefSeq ORF | 4098 bp |
| Locus ID | 218629 |
| UniProt ID | Q6PGC1 |
| Gene Summary | ATP-binding RNA helicase involved in translation initiation. Part of the 43S pre-initiation complex that is required for efficient initiation on mRNAs of higher eukaryotes with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211948 | Dhx29 (tGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 29 (Dhx29) |
CNY 14920.00 |
|
| MR211948 | Dhx29 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 29 (Dhx29) |
CNY 14008.00 |
|
| MR211948L3 | Lenti ORF clone of Dhx29 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 29 (Dhx29) |
CNY 15490.00 |
|
| MR211948L4 | Lenti ORF clone of Dhx29 (mGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 29 (Dhx29) |
CNY 15490.00 |
