Simc1 (NM_176987) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4732471D19Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224336 representing NM_176987 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACTTTATCGTGATCTCCGACGACAGTGGCTCCGAGAGCTCGGCCGGGACCCGCTCGGGCAGGG CGCGGAGGCTGCGCCGGGCCCTGTCTCGGACCCCCGGCGCGCTGCCCCGCCGAACCGTGGACTTCATTGA CTTAACTAGAGAAAACCGAGCAAGGACAAAAGACCGGAATGGACTCTGTGTGATTGATCTAACAAGAAAT GAGGAAGAAAATAGGCCTATTGCCACTCTTGATTTGACTTTAGAACCTGTAGCTTCCTCTCAGAAGGAGC CAACCAGTCTTCAGACATGTACCAGTCTCTCGGGCAAAGAAGTGATGGAAGCCCATGAAGATAGAGGCTC TCAGCCTGCAGCACAGAGAATCATTAACAATGATCCTGTAGATTTGGATTTGCTAGAGGAAAACCTGTTT GAAGGTTCTCGACCCCCTACATCTATCAGCCAGGACTCTGTTTATCCACCAGAACCAAACTGTAGCTCAA TCACATATAAAGGTGACCTCAGCTTCTTGACAAGCCTACAGCTCTCTTCAGATGTTAGCTCCTTCTCCTC CACAAGCAATCAGAGGGCCTCTTTGCCATGCCCACAGCAAGATGTGCCTTGTCAATCACAAGGCTTGCTA TGCCCGCTACAGGCCTTATCATGCCCACAAACAGCTTCACCATGTCCACCACGAGCTTCCTCATGCCCAC CACAAGCCCTGTCATGCCCACCTCAAGCTTTGTCATGCCCATCACAAACTTTACAGTGCCAACTCCAAGC TTTGCCTCAACCACCTCAAGAAGTGCCATGCTCTACCCAAAATGTACCGTGCCCTCAGCAGAATATGCCA AGCACACCTCAAGGCTTATCATGGCATCCTCGACACACCCTATACCCATATCAAGACACTCTGGGCCTAC CTCAAGATGTTCCAGGCCGACCTCAAAACATGTCATATCCACAAGATGTGACACAACTGCAAGACATGCC ATGGTCACTACATGACATGCCACTGTCACTACAAGATGTGTTACAGTCACTGCAAGATGTGCCACCATTG CTGGGAGATGTGCCGCCATCACCAGAAGTGATGCAGTTACCAGGATATGTGACACAGACATCAAGAGATG TGATTCAGTCACCGGCAGGTGTGACACAGTCACTAGGAAGTATGATGCAGTCACCAGGAAGTGTGACCCA GTCACTAAGAAGTGTGATGCAGTCATCAGGAAGTGTGACCCAGTCACTAAGAAGTGTGATGCAGTCATCA GGAAGTGTGACCCAGTCACTAAGAAGTGTGATGCAGTCATCAGGAAGTGTGATGCAGTCACCAGGAGGTG TGACACAGTCACTAAGAAGTGTGACGCAGTCACCAGGAGGTGTGATGCAGTCACCAGGAGGTGTAATGCA GTCACCAAGAGATGTGATGCAGTCACCAAGAGATGTGATGCAGTCACCAAGAGATGTGATGCAGTCACCA AGAGGTGTGACACAGTCACTAGGAAGTATGATGCAGTCACCAGGAGGTGTGATGCAGTCACTAAGAAGTG TAATGCAGTCATCAGGAGGTGTGACACAGTCACTAAGAAGTGTGATGCAGTCACCAGGAGGTGTGATGCA GTCACCAGGAGGTGTGACACAGTCACCAAGAGGTATGATTAAGTCACCAGGAATGATGCTGTCACCAGGA GATGTGATACAGTCACTAAACAGTGTGCCACAGTCATCAAGAGATAGGATGCAGTCAGCAGGACATGTGC CATCAGCATCAGGAGATGCAATACAATCACCAGGAGGTATGTCACCGACATCAAGAGATAGGATGCAGTC ACCAGGAGGTGTGTCACTGGCAACAGAAGATTCGATACAGTTACCAGGAGGTGTGCCACTATCATCAGAT GTGATACAGTCACAAGGAGGTGTGCCATTATCATCAAAAGATAGGATGCAGTCACCAGGAGGTGTGCCAC CATCATCTGGAGATATGATACAGTCACAAGGAGGTGTGCCACAATCATTAGGAGATGCAATACAGTCAGC AGGAGGTGTGCCACAATCATCAGGAGATGCGATACAGTCACCAGGAGGTGTGTCACTGGCAACAGGAGAT TCGATACAGTTACCAGGAGGCGTGCCACTATCATCGGGAGATGTGATACAGTCCCAAGGAGGTGTGCCAC GGTCATCAAGGGATAGGATGCAGTCACCAGGAGGTGTGCCACCATCATCTGGAGGTGTGATACAGTCACC AGGAGGTGTGTCACCAGCATCAGGAGATGCAATACAGTCACCAGGAAGTGTGTTACCGGCATCAGGAGAT GCAATACAGTCAGCAGGAGGTGTGTTACTGGCATCAGGAGATGCAATACAGTCACCAAGAAGTGTGCCAC AGTCACCTAGCGGTACATTGCGGTCACCAGGCAATATGTCAGAGTCACTTGGAGATACACCAAACTTATC AGGAGATGTGTCAAACTCACCACAAGCATTATTGGACTTAGCAAGAGACAGGCCGAAGTCTTCCCCGAAT GATGTGCAGAATCGTGATACTCCTATGGATATCTCAGCTTCATCCTCTTCAAGTTGCTCTGCTAACCCAC AATCTCGACAGTCTGAATTTAAATTAGATAAAGTTCCTTGGCTCACTATCACAGATAGCTCAGCCAGGAG AGAGAAATCACTGCCACAGCTTGCCAACCCTGGGTCTGCTCAGATACAAGGACAAATACCACAAGTTGGG GTATACAATAGACCTTGCCTGCATAGACTGAAATACTTCTTACGGCCTCCAGTACATCATCTCTTCTTCC AGACATTAATACCAGATAAAGATACACGAGAGAGCAAGGGTCAGAAATTAGAACCCATCCCTCATCGAAG ACTAAGAATGGTGACAAACACTATTGAAGAAAACTTCCCTTTGGGGACTGTGCAGTTTTTAATGGACTTT GTTTCACCCCAACATTACCCACCCAGAGAAATTGTGGCTCACATCGTCCAGAAAATCCTGCTCAGTGGCT CTGAGACTGTGGATGTCCTCAAGGAGGCCTACATGCTTCTCATGAAAATCCAACAGCTGCATCCAGCCAA TGCCAAGACAGTGGAGTGGGACTGGAAACTGCTCACCTATGTCATGGAGGAAGAGGGACAAACTCTGCCT GGACGAGTTCTCTTTCTACGCTACGTAGTGCAAACCCTGGAAGATGACTTCCAGCAGATCTTGAGGAGAC AGCGGCAGCACCTACAGCAATCCATTGCAAACACTGTTCTGTCATGTGACAAACAGCCTCACAATGTCAG AGATGTTATTAAGTGGCTGGTCAAAGCAGTGACTGAAAATGAATTAACTCCACCCCAAGATGAGACTCAG ACCTCTCCAAGAACAGGAGTCTTGAAAACTAGCAGTGACCATCTGTCTCCCCGGCCTAACCAGAACAAGA ACACCAATCAACTGATTGTGTGTCAGCTTCAGAGGATGTTGTCCATCGCTGTAGAGGTGGACAGGACTCC AACCTGCAGCTCCAATAAAATAGCGGAGATGATGTTTGGGTTTGTGCTGGATATCCCTGAGAGGAGCCAG AGGGAGATGTTCTTTACTACCATGGAAAGCCACCTTCTGCGATGCAAAGTCCTGGAGATCATATTCCTGC ACAGCTGTGAGACACCCACCCGTCTCCCCTTGTCTCTGGCCCAGACCCTCTATTTTCTGAACAACTCCAC GTCCCTGCTCAAGTGTCAATCAGATAAAACGCAGTGGCAGACATGGGATGAGCTGGTTGAACATCTGCAG TTCCTGCTGTCCAGTTATCAACACGTGCTGAGAGAGCACCTACGGAGCTCAGTGATTGATCGGAAAGACT TAATCATCAAAAGAATTAAGCCCAAGCCCCAACAAGGAGATGACATCACGGTGCTGGATGTGGAGAAGCA GATCGAGGCCTTCCGCAGCCGCCTGGTCCACATATTGGGAGAACCTTTAGTCCCCCAGCTCCAAGACAAA GTGCATTTGTTGAAGCTGCTGCTCTTCTATGCTGCGGACTTGAACCCCGACACAGAGCCTGCTTCAGAGC ACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_176987
Insert Size	4065 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_176987.4, NP_795961.3
RefSeq Size	4811 bp
RefSeq ORF	4065 bp
Locus ID	319719
UniProt ID	E9Q6E9
Gene Summary	Inhibits the protease activity of CAPN3.[UniProtKB/Swiss-Prot Function]

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