Eif3a (NM_010123) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A830012B05Rik; Csma; Eif3; Eif3s10; mKIAA0139
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224280 representing NM_010123 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCCTATTTCCAGAGGCCGGAAAATGCCCTCAAGCGCGCCAACGAATTTCTTGAGGTTGGCAAAA AGCAGCCTGCCCTGGATGTTCTTTATGATGTAATGAAAAGTAAGAAGCACAGAACATGGCAGAAGATACA TGAGCCAATTATGCTGAAATACTTGGAACTGTGTGTGGATCTTCGTAAGAGCCACTTGGCTAAGGAAGGG TTATATCAATATAAGAACATATGCCAACAGGTAAACATTAAATCTTTAGAAGATGTTGTTAGGGCATATT TGAAATTAGCAGAGGAAAAAACAGAAGCTGCTAAAGAAGAGTCCCAACAAATGGTGTTAGATATAGAAGA TCTGGATAATATTCAGACTCCTGAGAGTGTTCTCTTAAGTGCAGTAAGTGGGGAAGATACTCAGGATCGT ACTGACAGATTGCTACTGACTCCCTGGGTCAAGTTCCTGTGGGAATCATACAGGCAGTGTTTGGACCTTC TTCGAAACAATTCTAGAGTAGAGCGCCTTTACCATGATATCGCCCAACAAGCTTTCAAATTCTGCCTCCA GTATACTCGGAAGGCTGAGTTCCGCAAGCTATGTGACAACTTGCGAATGCACTTATCCCAGATTCAGCGC CACCATAACCAAAGCACAGCAATTAATCTTAATAATCCAGAGAGCCAGTCTATGCATTTGGAAACCAGAC TTGTTCAGTTGGACAGTGCTATCAGCATGGAATTATGGCAGGAAGCCTTCAAAGCTGTGGAAGATATTCA TGGACTATTTTCCTTGTCTAAGAAACCACCTAAGCCTCAGTTGATGGCAAATTACTATAACAAAGTTTCA ACAGTGTTTTGGAAATCTGGAAATGCTTTGTTCCATGCATCTACACTTCATCGTCTTTATCATCTGTCTA GAGAAATGAGAAAGAATCTTACACAAGAAGAGATGCAAAGAATGTCTACTAGAGTCCTTTTGGCGACTCT TTCCATTCCTATTACTCCTGAGCGAACTGATATTGCTCGACTTTTGGATATGGATGGTATTATAGTTGAG AAGCAGCGACGCCTTGCCACATTGCTAGGTCTTCAAGCCCCACCCACACGAATCGGCCTAATTAATGATA TGGTCAGGTTCAGTGTGCTACAGTATGTTGTCCCAGAAGTGAAAGACCTTTACAACTGGTTGGAGGTGGA ATTCAACCCACTAAAACTCTGTGAGAGAGTTACAAAGGTATTAAATTGGGTTAGGGAACAACCTGAAAAA GAACCAGAATTGCAACAATATGTACCACAACTCCAGAACAATACCATACTCCGCCTTCTGCAACAGGTGG CACAGATTTATCAGAGCATTGAGTTTTCTCGTTTGACTTCTCTGGTTCCTTTTGTTGATGCTTTCCAACT GGAACGGGCCATAGTAGATGCAGCCAGGCACTGTGACCTGCAGGTACGTATTGACCATACTTCCCGGACT CTGAGTTTTGGATCAGATTTGAATTATGCAACTCGAGAAGATGCCCCAGTTGGCCCTCATCTGCAGAGCA TGCCTTCAGAGCAGATAAGAAACCAGCTCACGGCCATGTCCTCAGTGCTTGCCAAAGCACTTGAAGTCAT CAGACCTGCTCACATTCTGCAAGAGAAAGAAGAACAGCATCAATTGGCAGTTAATGCATATCTTAAAAAT TCAAGAAAAGAGCACCAGAGGATCCTGGCTCGGAGACAGACAATTGAGGAAAGAAAAGAGCGTCTTGAGA GTCTGAATATTCAACGTGAGAAGGAAGAACTCGAGCAGAGGGAAGCTGAACTCCAGAAAGTACGAAAGGC TGAAGAAGAAAGGCTGCGCCAAGAGGCAAAGGAGCGAGAGAAGGAACGAATCCTTCAAGAACACGAGCAA ATCAAGAAGAAAACTGTTCGGGAGCGGTTAGAGCAGATCAAGAAGACAGAGCTGGGCGCCAAAGCATTTA AAGATATTGACATTGAAGACCTTGAAGAACTGGATCCAGATTTTATTATGGCCAAACAGGTTGAACAACT GGAGAAAGAAAAGAAGGAACTTCAGGAACGCCTGAAGAATCAAGAAAAGAAGATTGACTATTTTGAGAGA GCTAAGCGTTTGGAAGAAATTCCTTTAATAAAGAGTGCTTATGAGGAACAAAGGGTTAAAGACATGGACC TGTGGGAACAGCAAGAAGAAGAAAGAATCACTACAATGCAGCTAGAACGAGAAAAAGCTCTGGAGCATAA GAATAGGATGTCACGAATGTTGGAAGACAGAGATCTATTTGTGATGCGCCTCAAAGCTGCCCGGCAGTCT GTCTACGAGGAAAAACTGAAACAGTTTGAAGAGCGCTTAGCAGAAGAAAGGCATAGTCGCCTAGAAGATC GGAAAAGGCAGCGGAAAGAAGAACGCAAAATAACTTATTACAGAGAAAAGGAAGAAGAAGAGCAGAGGAG GGCAGAGGAGCAGATGCTCAAAGAGCGAGAAGAAAGAGAACGTGCTGAGAGAGCAAAACGGGAGGAAGAA CTTAGAGAGTATCAGGAGCGAGTCAAGAAACTAGAAGAAGTAGAAAGGAAAAAGCGGCAAAGAGAGCTGG AAATTGAAGAAAGGGAAAGGCGCAGAGAGGAAGAAAGGAGACTTGGTGATGATCCACTTTCTAGGAAGGA CTCTCGGTGGGGAGATAGAGATTCAGAAGGCACCTGGAGGAAAGGACCAGAAGCTGACTCTGAGTGGAGA AGAGGCCCACCAGAAAAGGAGTGGAGACGAGAAACTCGGGATGATGAGAGGCCTCACAGGAGAGATGAGG ATCGGCTGAGGCGCTTGGGGGGTGACGATGAAGAGAGAGAGTCTTCTCTTAGACCGGATGATGATCGTAT CCCCAGGCGTGGCCTGGATGATGACAGAGGGCCTAGACGTGGTCCCGATGAAGATAGATTTTCTCGTAGA GGAACAGATGATGACCGTCCTTCCTGGCGTAATGCAGATGATGATAGGCCTCCCAGGCGAATTGGTGATG ATGACAGGGGAAGCTGGCGTCACACAGATGACGACAGACCACCCAGACGGGGACTGGATGATGAGAGAGG AAGCTGGCGTACAGCAGATGAGGACAGAGGACCTAGACGAGGGATGGATGATGACCGGGGGCCAAGACGA GGAGGTGCTGATGATGAACGGTCATCCTGGCGGAATGCTGATGATGATAGGGGTCCCAGGAGAGGCATGG ATGATGATAGGGGTCCCAGGCGAGGGCTGGATGATGACCGAGGACCTTGGAGGAATGCTGCTGAAGATAG GATTTCCAGGAGAGGTGCAGATGATGACCGAGGGCCTTGGAGAAATATGGATGATGATAGGGTTCCTAGA AGGGGTGATGATGCAAGACCTGGTCCTTGGAGACCATTTGTCAAGCCAGGTGGATGGAGAGAGAAAGAAA AGGCTAGAGAAGAGAGTTGGGGTCCACCTCGAGAATCAAGACCATCAGAAGAACGTGAATGGGATAGAGA CAAAGAGAAGGACAGAGATAATCAAGATCGAGAGGAGAATGACAAAGACCTTGAACGAGATAGGGACAGA GAGAGAGATGGGGACCGGGAGGATCGATTCAGAAGACCCAGGGATGAAGGTGGCTGGAGAAGAGGACCAG CAGAAGAATCTTCAAGCTGGAGAGATTCAAGTCGCCGTGATGATAGGGACAGGGAAGACCGTCGACGGGA TAGAGATGATCGTCGTGATCTGAGAGACCTGAGAGACAGAAGGGATTTAAGAGATGATAGAGATCGGAGA GGACCTCCCCTCAGATCAGAGCGAGAAGAAGCAAGCTCTTGGAGACGCACTGATGACAGGAAAGATGACC GGACTGAAGAGAGGGATCCACCTCGTCGTGTTCCTCCCCCAGCTCTTTCAAGAGATCGAGAAAGAGAGCG AGAACGAGAAGGTGAGAAAGAGAAAGCATCCTGGAGAGCTGAGAAAGATAGGGAGTCCCTTCGTCGTACT AAGAATGAAACTGATGAAGATGGATGGACCACAGTACGACGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010123
Insert Size	4035 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010123.3, NP_034253.3
RefSeq Size	5176 bp
RefSeq ORF	4035 bp
Locus ID	13669
UniProt ID	P23116
Gene Summary	RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.[UniProtKB/Swiss-Prot Function]

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