Rpgrip1 (NM_023879) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610005A07Rik; 4930401L23Rik; 4930505G06Rik; A930002K18Rik; AA415034; nmf247
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224268 representing NM_023879 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCATCTACTAGAATATATGCCAGAGGATCTGCCAGTTAGAGACACAGATTCGAGTCCTCTGTTGA AAGGGACGTCGGGTAAGAATGTAAGGGCCCAGCCGCACTTGGGCAGGATGAACCAGAAGGAGCTGAACTG CAGACGCCTTCACCTCCATGAAGAGCCCACGTTGGTGAAGGAACCTTCTCCAAAGCAACGAGATAAAAAC AGAAGGCGAAGGACGAACGTGCAGCGATCCACAACTACCCAACCGGACCTACGGACACTTGCTGTGCTCC AGGAGCCAGAGAGGCGCAGGCGGCCGTGGGTCTCAGCGTCCCCGAGCCCCTCGGCGCCCCCGCGCGCACC AGTTCCCGGGAGGAAAGCGCATGTGCAGCGCCTGTGTCCCTCGACCGCGGTAGGCAGTGCACAGCCTCGA GTCCACGCGGGACGCAGACTGCCCCACATAGCTGGGCCAAATGACAGGCGCAGCCATACAGCCCCTCCCG CATTTAAAGACTATGTGGCGGACAAGAACACCAGAATCGAAATAACCAGGGAGCCCAGCCAACTTACTCA CACCATGACGACTGATTCTACACATGTGGAAGAGATCCCCAGATCTCCTGAGAAAACATCCAAAGTTGAA AAGCCTGAACAGAGAAGCTCAGAGGAGTGCACTCAGAAGGCTGCAGAGCTTCGAGCTTCCATTAAGGAGA ACGTGGAGCTGATTCGCCTTAAGAAGCTCTTACAGGAGAGGAATACCTCGCTGGCAGCCACAGAAGCCCA ACTGACACGAGTTCAAGAGGCATATGAAGACTTGCTTCAAAAGAATCAGGGAATATTGGATACAGCCCAT AATGCCTTCCTCAGCCAAGTGAATGAGCTCAAGGCAGAGCTGAGTGAAGAGAGCAAGAAGGCTGTGAGCT TGAGGACTCAGCTGGGAGATGTGTCTATCTTGCAGATAACTCTGAAGGAGTTTCAGGTCAGAGTTGAAGA TTTGGAAAAGGAACGGAAACTGCTGAGTGACAGTTATGACAGACTCTTAGAGAACATGCTGGATAGTAGT CATCAGCCTCTGGATAGCAGTCATCAGCCTCACTGGAGCACGGAGCTCACAGGGAAGCAGCTCCCACCGC AAGTCTGTCCACTGCTGGACCAAATGGGCACTGCGCTGGAGGAGACCAAAGTCTTTCGTCAAGCAACCAA CAAAGCAGCTCAAGATGGAAAGCTGAAGTTTCAAGACACCGACATCCTTTACCAGCATGAACAGGAAGAA GAGAGCCTTCAGTCAACTGCCACTGTAGCCTCATCTCCAGAAGAGCTGTGTGAACTGGCTGCTCAGCCCA CTCTACTCCCACAGACAGATCAGAGAGAGTCCAGTGAACCAAAAGCTCAAGACGAGAATGACTTATCCCA GGTGCTAAGCGAGCTGCAAGTGTCACATGCAGAGACCACACTGGAACTGGAAAAAACCAGAGATATGCTT CTTCTGCAGCGCAAAATCAACATGTGTTATCAGGAGGAGCTGGAGGCAACACTGACAAAAGCTGACAGGG AAAACAGGGACCACGAAGAGAAACTGGAGCGGCTGAATCACCTCCTGGACTTCAAGAACAGCCGCATCAA ACAGCTGGAAGGTATTTTAAGAAGCCACGGCCTTCCAACATCTGAGCAGCTCAAAGATGTCGCGTATGGC ACTCTGCCACCGTCGCTGTGTCTGGAGCCCCTGGCAGCCCACAGAGGTGACGATGAAGTGGACATGTCTC TGCTGCATCCAAGCGAGAATCTTTTTGAGCTGCACGTCCACCAGGCCTTCCTGACCCCTGCTGCCCTCAC ACAGGCTGGCGACACCCAACCTACCACTTTCTGCACTTATTCCTTCTATGATTTTGAAACTCACTGCACC CCACTCTCTACTGGGCCCCAGCCTCTCTATGACTTCACCTCCCAGTATGTAGTGCAGGCAGACTACCTCC TCCTGCACTACCTTCAAGGAACTTCAGTACGGCTTGATCTCCATCAGGCCATGGCTAGTGAATACCATGT CCTTGCTACTGGATGGATCTCACTTGACAAGGTGCTAGGGACTGTGGAGAGAGTCCACGGCTTGGCCACA CTCGCTGGAGCTGGTGGAGAAGACTTGGGGGTGTTAGAGTACTGGATGAGGCTGTGCCTGCCCCTAAAAC CCAGCCTCCAGGCATGCAATAAGCGAAAGAAAGCCCAGGCCTACCTGTCAGTCAGTGTGCTTGGAGCTCG GAAGGTCCAGAGCAATGAGTCCAGATCCGAAACCTGGGCGCCTCAGAATGAGCTTCGGGTTGAGATCACC AGGTGCTGCGGCCTCCGGAGTCGACGGCTGGGAAGGCAGCCCAGTCCGTATGTCATGTACCGCTTCTTCA CCTTTCCTGACCATGACACCATCATCATTCCAGCCAGCAGCAATCCATACTTTAAAGACCAGGCTCTGTT TCCCGTGCTTGTGACCTCTGACCTGGACCAGTATCTGAGGCGGGAAGCTCTGTCTGTCTATGTTTTTGAT GATGAAGACCCAGAGCCTGGCTCCTATCTTGGCCGAGCCCAGGTGCCCTTGCTGCCTCTTGCACAAAACA AATCTATCAAAGGTGATTTCAATCTCACAGACTCTGGGGAGAAGTCCAATGGATCCATCAAAGTGCAACT AGACTGGAAGTCTCACTACCTAGCCCCAGAGGGCTTCCAGATGTCAGAAGCTGAGAAGCCGGAGGGTGAA GAGAAGGAGGAGGAAGGGGGAGAGGAGGAGGTAAAGGAGGAAGAGGTGGAGGAGGAAGAGGAAGAAGAAG AAGAGGAAGAAGAGGTCAAGGAAGAGAAGGAGGAAGAGGAGGAGGAGGAAAGGGAGGAGGAAGAGGAGAA GGAGGAGGAGAAGGAGGAAGAGGAGGAGGAGGACGAGAAGGAAGAGGAGGAAGAGGAAGAGGAAGAAGAG GAGGAAGAGGAGGAGGATGAGAACAAAGATGTCCTGGAGGCCTCATTCACGGAAGAATGGGTCCCCTTTT TTTCCCAGGACCAGATAGCATCTACAGAGATTCCCATAGAGGCTGGCCAGTACCCAGAGAAGAGAAAACC TCCTGTAATAGCAGAAAAGAAGGAAAGAGAGCATCAGGTCGCAAGCTACTCACGAAGAAAACACAGCAAA AAACCAGGTGTCCAAGACAAGAACAGAATGGAATATCTGAGCTGTAACATCTTGAATGGAAACACACAGC AGATGCATTACACGGAGTGGAAGTTCTCAGGGCTCAAGAAGGCCGAGGATGGTGGCTTAAAAGCTCAGGA CAAGAGAGAGGAACCGCCATCACCCCGTTCAGCACTGAGACAGGAGCATCCCTCACATCCTAGAAATGCC TTCTCCCTAGCAGACCAGGAATCCTGTGAACAAGCTTCTGAAGTCAGTGAAACACAGACTACCGACAGCG ATGACATCATAGTGACACCCCAGGCTCAGACGGTTCCTAAAGCCGATTCAGAGAAGATGTGCATTGAAAT CGTCTCACTGGCCTTCTGCCCTGAGGCTGACGTCATGTCTGATGAGACTATCCAGCAGGTATATGTGGAG TACAAATTCTGTGATCTGCCTCTATCGGAGACAGAGACTCCGATGTCCCTGAGGAAACCAAGGGCAGGCG AAGAAATCCACTTCCACTTTAGCAAGGTGATAGATCTGGACCCTGTGGAGCACCAAAGCCGAAGGCAGTT TTTGTTTGCCATGCTACATGCTCAAGATTCTGATGAGGGACGTTTCAAGTTTACAGTGGTAAGTGATCCT CTGGATGAAGAAAAAAAAGAATGTCAAGATATAGGCTATGCATACCTTGAACTGTGGCAGATATTTCAAT CTGGAAAAGATATCCTAGAGCAAGAACTAGAGATTGTGAGCCCTAGAAACCAGGCCATCCAAATTGGAAG GCTGAAGGTTTCCCTCCAAGCAGCTGCTGCCCTCCATGGCATTTATAAGGAGATGACTGAAGATCTGTTT TCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_023879
Insert Size	3996 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_023879.3, NP_076368.1
RefSeq Size	5238 bp
RefSeq ORF	3996 bp
Locus ID	77945
UniProt ID	Q9EPQ2
Gene Summary	May function as scaffolding protein. Required for normal location of RPGR at the connecting cilium of photoreceptor cells. Required for normal disk morphogenesis and disk organization in the outer segment of photoreceptor cells and for survival of photoreceptor cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant represents the longer transcript and it encodes the longer protein (isoform 1).

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