Ehmt1 (NM_001012518) Mouse Untagged Clone
CAT＃: MC224190
Ehmt1 (untagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1, (10ug)
CNY 16720.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9230102N17Rik; D330003E03; Eu-HMTase1; GLP; GLP1; KMT1D; mKIAA1876 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224190 representing NM_001012518
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCGCTGATGCTGAGCAGGCAGTTCTGGCCAAGCAAGAGACCAAGCAGGATTGCTGCATGAAAA CTGAGCTGCTAAGGGAAGATACACCTATGGCTGCTGATGAAGGTTCCACAGAGAAACAAGAAGGAGAGAC TCCCATGGCTGCAGATGGAGAAACAAATGGGTCTTGTGAAAAGAGTGGGGATCCCAGCCATCTAAATGCA CCCAAACACACTCAGGAGAACACAAGAGCTAGCCCACAGGAAGGCACCAACAGAGTGTCTCGGGTGGCAG AAAATGGGGTTTCAGAAAGAGACACAGAAGTGGGGAAGCAAAACCATGTCACAGCTGACGACTTCATGCA GACATCTGTCATTGGCAGCAATGGATATTTCTTAAATAAACCAGCCCTGCAGGGGCAGCCGTTGAGGACT CCCAACATTCTAACCTCCTCGCTTCCTGGTCATGCTGCAAAAACTCTTCCTGGAGGAGCCAGTAAATGCA GGACTCTGAGTGCACTTCCTCAGACACCAACCACAGCACCCACTGTGCCTGGGGAAGGGAGTGCAGACAC AGAGGACAGAAAGCCTACAGCCTCGGGCACTGATGTCAGGGTTCACAGGGCACGCAAGACCATGCCGAAG TCCATCTTGGGCCTGCATGCAGCCAGCAAAGACCATAGAGAAGTTCAAGACCATAAGGAACCAAAAGAGG ATATCAACAGAAACATTTCTGAATGTGGACGACAGCAGCTTTTACCAACCTTCCCAGCCCTCCACCAGTC GCTACCTCAGAATCAGTGCTACATGGCCACCACAAAGTCCCAGACAGCTTGCTTGCCTTTTGTTTTAGCA GCTGCAGTATCTCGGAAGAAAAAACGAAGAATGGGAACCTATAGTTTAGTTCCCAAGAAAAAGACCAAAG TATTAAAACAGAGGACGGTGATTGAGATGTTTAAAAGCATAACCCATTCCACTGTGGGCGCCAAGGGCGA GAAAGCCTTAGATGATAGTGCCCTGCATGTAAATGGCGAGAGCTTGGAGATGGACTCAGAAGATGAAGAC TCCGATGAGCTGGAGGATGACGAGGACCATGGAGCTGAGCAAGCGGCTGCATTTCCCACCGAGGATAGTA GGACTTCTAAAGAGAGCATGTCTGAGACTGACCGGGCTGCAAAGATGGATGGAGATTCAGAGGAGGAACA GGAGTCTCCCGACACAGGGGAGGATGAAGATGGTGGAGACGAGTCTGACCTGAGTTCTGAATCCAGTATC AAGAAGAAATTTCTCAAGAGGAGAGGGAAGACTGACAGCCCCTGGATCAAACCTGCTCGGAAAAGGAGGC GGAGAAGTAGAAAGAAGCCGAGCAGCATGCTTGGTTCCGAGGCTTGTAAGTCATCTCCAGGAAGCATGGA GCAGGCAGCTCTGGGAGACAGCGCTGGCTATATGGAAGTTTCCCTGGATTCCCTGGATCTCCGTGTCAGA GGAATTCTGTCTTCCCAGACAGAAAATGAAGGGCTGGCCAGTGGTCCGGATGTGCTGGGGACGGATGGCC TCCAGGAAGTGCCTCTCTGCAGCTGCCGAATGGAAACCCCCAAGAGCCGCGAGATCAGCACCCTGGCCAA CAACCAGTGCATGGCCACTGAGAGCGTGGATCACGAATTGGGCCGGTGTACAAACAGCGTGGTCAAGTAT GAGCTGATGCGCCCATCCAACAAAGCACCGCTCTTGGTGTTATGTGAAGACCATCGGGGTCGCATGGTGA AGCACCAGTGCTGTCCTGGCTGCGGCTACTTCTGCACGGCGGGTAACTTCATGGAATGCCAGCCTGAGAG CAGCATCTCTCATCGTTTCCATAAGGACTGTGCCTCTCGAGTCAACAATGCCAGCTACTGTCCCCATTGT GGGGAAGAAGCTTCCAAGGCCAAAGAGGTGACCATAGCAAAAGCAGACACAACTTCCACAGTGACCCTAG CCCCTGGACAGGAGAAGAGCCTGGCTGCTGAAGGCAGGGCTGACACGACCACGGGCAGCATTGCTGGAGC CCCAGAGGATGAAAGATCGCAGAGCACAGCCCCCCAGGCACCAGAGTGCTTCGACCCTGCCGGACCGGCT GGGCTCGTGAGGCCGACATCTGGCCTTTCCCAGGGCCCAGGAAAGGAAACCTTGGAAAGTGCTCTAATCG CTCTAGACTCTGAAAAACCCAAGAAACTTCGCTTCCACCCAAAGCAGCTGTACTTCTCTGCCAGGCAGGG TGAGCTGCAGAAGGTGCTTCTCATGCTGGTTGATGGAATTGATCCCAACTTCAAAATGGAGCACCAAAGT AAGCGTTCCCCATTACATGCTGCTGCGGAGGCTGGCCACGTGGACATCTGCCACATGCTGGTTCAGGCGG GTGCCAATATTGACACTTGCTCAGAGGACCAGCGGACCCCACTGATGGAGGCTGCAGAGAACAACCACTT GGATGCAGTGAAGTACCTCATCAAGGCTGGAGCACAGGTGGATCCGAAGGACGCAGAGGGCTCCACATGT TTGCATTTGGCTGCCAAGAAAGGCCACTATGATGTGGTTCAGTATCTGCTTTCAAATGGACAGATGGATG TCAACTGCCAGGATGACGGTGGATGGACACCTATGATCTGGGCCACTGAGTACAAGCACGTGGAGCTGGT GAAGCTGCTGCTGTCTAAGGGCTCTGACATCAACATCCGGGACAACGAGGAGAACATTTGTCTGCACTGG GCAGCATTTTCAGGCTGTGTGGACATAGCTGAAATACTTCTGGCTGCCAAGTGTGACCTGCATGCTGTGA ATATCCATGGAGACTCACCCCTGCACATCGCAGCCAGGGAGAATCGCTACGACTGTGTTGTCCTCTTTCT TTCTCGGGATTCAGATGTTACTCTGAAAAACAAGGAAGGAGAGACTCCCTTGCAGTGTGCAAGTCTCAGT TCGCAGGTGTGGAGTGCATTGCAGATGAGCAAAGCACTTCGGGACTCAGCCCCTGACAAGCCCGTTGCTG TTGAGAAGACGGTGAGCAGGGATATCGCTCGAGGGTATGAGCGCATTCCCATTCCCTGTGTCAATGCTGT GGACAGTGAGCTGTGTCCTACCAACTATAAGTATGTCTCCCAGAACTGTGTGACATCCCCCATGAACATT GACAGGAACATCACTCATTTGCAGTACTGCGTGTGTGTAGATGACTGCTCCTCTAGCACCTGCATGTGTG GCCAGCTGAGCATGCGCTGCTGGTATGATAAGGATGGCCGACTTCTGCCTGAGTTTAACATGGCAGAACC ACCCTTGATCTTCGAGTGCAATCATGCCTGCTCATGCTGGAGGAACTGCCGCAATCGTGTGGTGCAAAAT GGTCTCAGGGCAAGGCTGCAGCTTTATCGGACACAGGACATGGGCTGGGGTGTGCGGTCCCTCCAGGATA TCCCACTGGGCACCTTTGTCTGCGAATACGTAGGGGAGCTGATTTCGGACTCTGAAGCTGATGTTCGGGA AGAGGACTCTTACCTCTTTGATCTTGACAATAAGGATGGAGAGGTATACTGCATTGACGCTCGGTTCTAT GGGAATGTCAGCCGGTTCATAAACCACCACTGCGAACCCAACCTTGTGCCTGTGCGAGTGTTCATGTCAC ACCAGGACCTGCGGTTTCCCAGGATTGCCTTCTTCAGTACCCGCCTGATTCAGGCTGGGGAGCAGCTCGG GTTCGACTACGGGGAGCGCTTTTGGGACGTCAAGGGCAAGCTCTTCAGTTGCCGGTGTGGGTCTTCCAAG TGTCGGCACTCAAGCGCAGCCCTGGCCCAGAGGCAAGCCAGTGCAGCCCAGGAGCCTCAGGAGAATGGCC TTCCAGATACCAGCTCTGCAGCCGCTGCTGACCCCCTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001012518 |
| Insert Size | 3891 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001012518.3, NP_001012536.2 |
| RefSeq Size | 5107 bp |
| RefSeq ORF | 3891 bp |
| Locus ID | 77683 |
| UniProt ID | Q5DW34 |
| Gene Summary | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227091 | Ehmt1 (tGFP-tagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1) transcript variant 1, (10ug) |
CNY 12540.00 |
|
| MR227091 | Ehmt1 (Myc-DDK-tagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1 |
CNY 9024.00 |
|
| MR227091L3 | Lenti ORF clone of Ehmt1 (Myc-DDK-tagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1 |
CNY 13300.00 |
|
| MR227091L4 | Lenti ORF clone of Ehmt1 (mGFP-tagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 1 |
CNY 13300.00 |
