Gigyf2 (NM_146112) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610016F01Rik; A830080H02Rik; AI852361; AW259676; BC006835; mKIAA0642; Tnrc15
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224175 representing NM_146112 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCAGAAACACAGACACTAAACTTTGGGCCTGAATGGCTCCGTGCTCTGTCCAGTGGTGGGAGTA TCACGTCCCCTCCTCTTTCTCCGGCCCTGCCAAAGTATAAATTAGCAGATTATCGATATGGCCGAGAAGA AATGTTAGCACTTTTCCTTAAAGACTACAAGATACCCTTTGATCTTCTGGAGAAAGAATTCCTGCCTATC CTACAGGAGGAGCCCTTGCCACCATTGGCTCTAGTACCCTTTACAGAAGAAGAGCAGAGAAACTTTTCCA TGTCTGTAAATAGTGCCGCTGTCCTGCGATTGACAGGACGAGGAGGAGGAGGAGGAACAGTGGTGGGGGC TCCTAGAGGTCGAAGTTCTTCAAGAGGGCGAGGCAGAGGTAGAGGTGAATGTGGTTTCTACCAAAGAAGT TTTGATGAAGTAGAGGGTGTGTTTGGGCGAGGAGGTGGCAGGGAAATGCATAGATCACAGAGCTGGGAAG AAAGGGGTGACAGGCGCTTTGAAAAACCAGGAAGAAAAGATGTAGGGAGACCAAATTTTGAGGAAAGTGG ACCAACGTCTGTGGGGAGAAAGCATGAATTTATACGGTCAGAAAGTGAAAACTGGCGAATCTTTAGAGAG GAACAGAATGGAGAAGATGAAGATGGAGGTTGGCGGTTAGCTGGATCAAGAAGGGATGGAGAGAGATGGC GGCCTCACAGTCCTGATGGCCCTCGTTCTACAGGCTGGCGGGAACACATGGAACGCCGTCGGAGGTTTGA GTTTGATTTTCGAGACCGAGATGATGAGCGTGGTTACCGAAGGGTGCGCTCTGGCAGTGGGAGCATAGAT GACGACAGGGATAGCTTGCCGGAGTGGTGCTTAGAGGATGCTGAGGAAGAAATGGGCACGTTTGACTCTT CTGGGGCATTCCTCTCTCTAAAAAAAGTGCAGAAAGAACCTATTCCTGAAGAGCAGGAAATGGACTTTAG GCCTGTGGAGGAGGGAGAGGAGCGCTCTGACTCTGACAGCAGCCATAACGAAGAGGCCAAAGAACCGGAT AAGACAAACCGAAGAGAGGGGGAGAAAACAGACAGAGCAGGGGCTGAAGCTAGTGAGGAAGTACCTCAGA CGTCATTGTCATCTGCTAGACCAGGTACCCCTTCAGATCATCAGCCTCAAGAAGCCACACAGTTTGAGAG GAAAGATGAACCCAAAGCTGAACAAGTGGAAAAGGCTGAAGAAGAGAATCGGTCAGAAAATAGTCTCTCA GCCAAGGTCCCCAGCAGAGGGGATGAAACGGTGCCTGCCTCCCAGCAACCCTCCACACCGCTTCCTCCAG ACACAGCCTCTCCTCTCCTCATCCTGTCGCCTCCTGTTCCTACTCCTAGTTCTGCCTCTCGGCCAGTTGA GACAGCAGCCGTCGAAGCTCCTGGTATGAGCAGTGTTTCCACAGAGCCAGATGATGAAGAGGGCCTCAAA CACTTGGAACAGCAAGCTGAGAAGATGGTGGCTTATCTCCAAGACAGTGCACTAGACGACGAAAGATTGA CATCAAAACTGCAAGAGCACCGAGCTAAAGGAGTGTCAATCCCACTGATGCATGAAGCAATGCAGAAGTG GTATTACAAAGACCCTCAGGGGGAGATTCAAGGTCCCTTTAACAATCAGGAGATGGCCGAGTGGTTTCAG GCGGGCTATTTTACTATGTCTTTGCTGGTGAAGAGAGCATGTGATGAAAGCTTCCAGCCCCTTGGTGATA TCATGAAAATGTGGGGAAGGGTTCCTTTCTCTCCAGGCCCAGCTCCCCCTCCTCATATGGGAGAATTGGA CCAGGAACGCCTGACCAGACAGCAGGAACTCACTGCCTTATACCAGATGCAGCACCTCCAGTACCAGCAG TTCTTAATCCAACAACAGTATGCACAGGTTTTGGCTCAACAGCAGAAAGCAGCTTTGTCATCTCAGCAGC AACAACAATTGGCTCTTCTTCTGCAGCAGTTCCAGGCTCTGAAGATGAGAATGTCTGATCAGAACATCAT TCCCTCAGTAACTAGGTCTGTGTCAGTGCCAGATACTGGCTCTATCTGGGAGCTTCAGCCAGCAGCCTCA CAGCCTGCAGTTTGGGAAGGTGGTAGTGTATGGGATCTTCCCCTGGACACCACAGCACCAGGTCCTTCCC TGGAACAGCTTCAGCAGCTAGAGAAGGCCAAAGCTGCAAAGTTAGAACAGGAGAGAAGAGAGGCAGAAAT GAGGGCAAAAAGGGAAGAGGAGGAGCGAAAGAGGCAAGAAGAACTCCGAAGACAACAGGAGGAAATCCTT CGGAGGCAGCAGGAGGAAGAAAGGAAAAGACGGGAAGAAGAGGAACTTGCTCGAAGGAAGCAGGAGGAGG CTCTGCGGCGCCAACGGGAGCAAGAGATTGCGCTAAGGCGGCAGCGAGAAGAGGAGGAAAGACAGCAGCA GGAAGAAGCTCTTAGACGATTGGAGGAGCGGAGGAGAGAAGAGGAGGAAAGGCGGAAGCAGGAAGAACTG CTGCGCAAGCAGGAAGAGGAGGCTGCCAAATGGGCCCGGGAAGAGGAAGAAGCCCAGCGCCGACTGGAAG AGAACCGGCTGCGCATGGAGGAGGAGGCTGCCAGACTTCGGCATGAAGAAGAAGAAAGAAAACGGAAGGA ATTGGAGCTGCAGAGGCAGAAGGATCTGATGCGTCAGAGACAGCAGCAGCAAGAGGCTCTCCGAAGGCTG CAGCAGCAACAGCAGCAGCAACAGCTTGCACAAATGAAGCTTCCCTCTTCGTCAACGTGGGGTCAGCAGT CTAACACAGCAACATGTCAGTCCCAGGCCACGCTGTCATTGGCTGAGATCCAGAAGCTGGAAGAAGAACG AGAACGCCAGCTTCGTGAAGAGCAAAGACGTCAGCAGAGGGAATTGATGAAAGCTCTCCAGCAGCAGCAG CAGCAGCAGCAACAACAAAAACTCTCAGGTTGGGGGAATGTCAGCAAACCTGCAGGTACCACCAAGTCTC TACTAGAGATCCAGCAGGAAGAGGCCAGGCAGATGCAGAAGCAGCAGCAGCAGCAACAACAACAACAACA GCAACACCAGCAATCAAACAGAGCCCGGAACAGCACACATTCCAACCTGCATACCAGCCTTGGGAATTCT GTATGGGGCTCTATAAACACAGGTCCTTCTAACCAGTGGGCGTCTGAGCTAGTCAGTAGTATCTGGAGTA ATGCAGACACCAAAAACTCCAACATGGGGTTTTGGGATGATGCAGTGAAAGAGGTGGGACCTAGAAATTC AACAAATAAAAATAAAAACAACGCCAGTCTCAGTAAATCTGTAGGTGTGTCTAACCGGCAGAATAAGAAA GTAGAAGAAGAAGAAAAGTTGCTGAAGCTCTTTCAGGGAGTAAATAAAGCCCAAGATGGATTTACCCAGT GGTGTGAACAGATGCTTCATGCCCTTAATACGGCAAATAACTTGGATGTTCCCACGTTTGTTTCTTTCTT GAAAGAAGTGGAGTCTCCTTATGAAGTCCATGACTATACCAGGGCCTATTTAGGAGATACTTCCGAGGCC AAGGAGTTTGCCAAGCAGTTCCTTGAACGCCGTGCCAAACAGAAAGTCAACCAGCAGCGTCAGCAGCAGC AGCAGCAGCAGCAGCAGCAGGACTCTGTCTGGGGGATGAACCATAGTACACTCCATTCGGTATTTCAGAC CAATCAAAGCAACAACCAACAATCAAATTTTGAGGCTGTGCAAAGTGGCAAGAAGAAGAAAAAGCAGAAG ATGGTTCGAGCAGATCCCAGTTTATTAGGGTTTTCAGTCAACGCCTCGTCAGAGCGACTGAACATGGGTG AGATTGAGACTCTGGATGACTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_146112
Insert Size	3876 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_146112.4, NP_666224.3
RefSeq Size	5743 bp
RefSeq ORF	3876 bp
Locus ID	227331
UniProt ID	Q6Y7W8
Gene Summary	Key component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation (PubMed:26763119). In 4EHP-GYF2 the complex, acts as a factor that bridges EIF4E2 to ZFP36/TTP, linking translation repression with mRNA decay (PubMed:26763119). May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling, including IGF1 and insulin receptors (PubMed:12771153).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.