Bcr (NM_001081412) Mouse Untagged Clone
CAT＃: MC224137
Bcr (untagged) - Mouse breakpoint cluster region (Bcr), (10ug)
CNY 16440.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5133400C09Rik; AI561783; AI853148; mKIAA3017 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224137 representing NM_001081412
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGACTCGGTGGGCTTCGCCGAGGCGTGGAGGGCGCAGTTCCCGGACTCGGAGCCGCCGCGCATGG AGCTGCGCTCGGTGGGTGACATCGAGCAGGAGCTGGAGCGCTGCAAGGCCTCCATTAGGCGCTTGGAGCA GGAGGTGAACCAGGAGCGCTTCCGCATGATCTACCTGCAGACGCTGCTGGCCAAGGAGAAGAAGAGCTAC GATCGGCAGCGCTGGGGCTTCCGCCGTGCAGCGCAGCCCCCCGACGGCGCCGCCGAGCCCCGAGCGTCCG CCCCGCGCCCGCCGCCCGCGCCCGCCGACGGCGCAGACCCGGCGCCCGTCGAGGAGTCCGAGGCGCGGCC GGACGGAGAGGGGTCACCGAGTAAGGGGCGGTCAGCGTCCGCCCGCAGGCCAGCGGCTGCTGCATCGGCG GACCGGGACGACCGAGGGCCCCCCACCAGCGTGGCGGCGCTCAGATCCAACTTCGAGAAGATCCGCAAGG GACCTGCCCAGCCCGGCTCTGCCGACGCCGAGAAGCCCTTCTACGTGAACGTCGAGTTCCACCACGAGCG CGGCCTGGTGAAGGTCAACGACAAAGAGGTGTCCGACCGAATCAGTTCCCTGGGCAGCCAGGCCATGCAA ATGGAGCGCAAGAAGTCCCAGCAGAGCGCCGGGCAGGGCCTGGGGGAAGCACCCAGACCCCATTACCGTG GGCGCTCCTCGGAAAGCAGTTGCGGCCTTGACGGTGACTATGAGGATGCAGAGTTGAATCCCCGCTTCCT GAAGGACAACCTAATAAATGCCAACGGCGGCAACAGGCCCCCTTGGCCGCCCCTGGAGTACCAACCCTAC CAGAGCATCTATGTCGGAGGCATGATGGTGGAAGGGGAGGGCAAGAGCCCTCTCCTGCGAAGCCAGAGCA CTTCAGAGCAGGAGAAACGCCTAACCTGGCCTCGCAGGTCCTACTCGCCTCGGAGTTTCGAGGACAGCGG AGGTGGCTACACGCCGGACTGCAGCTCCAACGAGAACCTCACCTCCAGCGAGGAGGACTTTTCCTCTGGC CAGTCCAGCCGCGTGTCACCAAGCCCCACCACCTACCGCATGTTCCGGGACAAGAGCCGCTCACCCTCGC AGAATTCGCAGCAGTCCTTTGACAGCAGCAGCCCCCCGACGCCACAGTGCCAGAAGCGGCACCGGCAGTG CCAGGTTGTGGTGTCTGAGGCTACCATCGTGGGTGTCCGCAAGACTGGGCAGATCTGGCCCAGTGATGGG GACAGCACCTTTCAAGGGGAAGCAGATTCCTCGTTTGGAACACCACCTGGATATGGCTGTGCCGCAGACC AGGCTGAGGAACAGCGCCGGCACCAGGACGGGCTGCCCTACATCGATGATTCACCTTCTTCCTCGCCACA CCTCAGCAGCAAAGGCAGAGGCAGCCTGGCCTCAGGCGCCCTGGATCCCACCAAAGTGAGTGAGCTGGAT CTGGAAAAGGGCCTGGAGATGAGAAAGTGGGTTTTGTCCGGAATCCTGGCCAGCGAGGAGACGTACCTGA GCCATCTGGAGGCCCTGCTGCTGCCCATGAAGCCTCTGAAAGCCGCTGCCACCACCTCTCAGCCTGTGCT GACCAGCCAGCAGATTGAGACCATTTTCTTCAAAGTGCCTGAGCTCTACGAGATCCACAAGGAGTTCTAC GATGGGCTGTTCCCCCGAGTGCAGCAGTGGAGCCACCAGCAGCGTGTAGGGGACCTCTTCCAGAAGCTGG CCAGCCAGCTGGGTGTGTACCGAGCCTTTGTGGATAACTACGGAGTGGCCATGGAAACTGCAGAGAAGTG CTGTCAGGCCAACGCTCAGTTTGCAGAAATCTCTGAGAACCTGCGAGCCAGAAGCAACAAGGATGTCAAG GACTCAACTACCAAGAACTCTTTGGAAACTCTGCTCTACAAACCTGTGGACCGGGTGACGAGGAGCACGC TAGTCCTTCACGACTTGCTAAAACACACCCCGTCCAGCCATCCCGACCATTCCCTGCTGCAAGACGCCCT CCGAATCTCTCAGAACTTCCTGTCCAGCATCAACGAGGAGATCACGCCCCGCCGACAGTCCATGACGGTG AAAAAAGGAGAGCACCGGCAGCTGCTGAAGGACAGCTTTATGGTAGAGCTGGTGGAGGGGGCCCGCAAGC TGCGTCACATCTTCCTGTTCACTGACCTGCTCCTCTGCACCAAGCTGAAGAAGCAAAGTGGAGGCAAAAC CCAGCAATATGACTGTAAATGGTACATTCCGCTCACGGACCTCAGCTTCCAGATGGTGGATGAATTGGAG GCACTACCCAACATCCCTCTGGTGCCAGATGAGGAACTGGATGCCTTGAAGATCAAAATCTCCCAAATCA AGAGCGACATCCAACGAGAGAAGAGAGCAAATAAGGGCAGCAAGGTCATGGAGAGGCTGAGGAAGAAGCT GTCAGAGCAGGAGTCACTGCTGCTGTTAATGTCTCCCAGCATGGCCTTCCGCGTTCACAGTCGCAACGGC AAGAGTTACACGTTCCTTATTTCCTCTGACTATGAGCGAGCCGAGTGGAGAGAAAGCATCCGAGAGCAGC AGAAGAAATGTTTCAAAAGTTTCTCTCTGACATCTGTGGAGCTACAAATGCTGACCAACTCATGTGTAAA ACTTCAGACGGTTCACCACATTCCACTGACCATCAACAAAGAAGATGACGAGTCTCCTGGACTCTACGGC TTCCTGCATGTCATCGTCCACTCAGCCACCGGCTTTAAGCAGAGCTCAAATCTCTACTGCACCCTGGAGG TGGATTCCTTTGGATATTTTGTGAATAAAGCAAAGACTCGAGTCTACAGGGACACAACTGAGCCAAACTG GAATGAGGAGTTTGAGATAGAGCTAGAAGGCTCCCAGACCCTGCGGATACTATGTTATGAAAAGTGTTAC AACAAAATGAAGATGACGAAGGAAGATGGTGAGAGCGCAGACAAGCTCATGGGGAAAGGCCAGGTCCAGC TGGACCCCCAAACCCTACAGGACAGAGACTGGCAGCGGACTGTCATCGACATGAATGGAATTGAAGTGAA ACTCTCCGTCAAGTTCACGAGCAGGGAGTTCAGCCTGAAGAGAATGCCCTCCCGCAAACAGACAGGGGTC TTCGGTGTCAAGATTGCTGTAGTCACAAAGAGAGAGAGGTCCAAGGTACCTTACATTGTGCGCCAGTGTG TGGAAGAGATCGAGCGCCGGGGTATGGAGGAGGTGGGCATCTACCGAGTGTCTGGAGTGGCCACAGACAT CCAGGCACTGAAGGCAGCCTTTGATGTCAATAACAAGGATGTGTCCGTGATGATGAGCGAGATGGACGTG AACGCCATTGCCGGCACGCTGAAGCTCTACTTCCGTGAGCTGCCCGAGCCCCTCTTCACGGATGAGTTCT ACCCTAACTTCGCAGAAGGCATTGCTCTTTCAGACCCCGTCGCAAAGGAGAGCTGCATGCTGAACCTGCT GCTGTCCCTCCCAGAAGCCAACCTGCTCACCTTCCTCTTCCTCCTGGACCACCTGAAAAGGGTAGCTGAA AAGGAGACGGTGAACAAGATGTCCCTGCACAACCTCGCCACGGTCTTTGGCCCCACACTGCTTCGGCCCT CAGAGAAGGAAAGCAAGCTCCCTGCTAACCCCAGCCAGCCCATCACCATGACTGACAGCTGGTCCCTGGA GGTCATGTCCCAGGTCCAAGTGTTGCTGTACTTCCTGCAGCTGGAGGCCATCCCTGCCCCTGACAGCAAG AGACAGAGCATCCTGTTTTCCACTGAAGTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001081412 |
| Insert Size | 3813 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001081412.2, NP_001074881.1 |
| RefSeq Size | 6537 bp |
| RefSeq ORF | 3813 bp |
| Locus ID | 110279 |
| UniProt ID | Q6PAJ1 |
| Gene Summary | Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form. The amino terminus contains an intrinsic kinase activity (By similarity). Functions as an important negative regulator of neuronal RAC1 activity (PubMed:20962234). Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity (PubMed:17116687). Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222863 | Bcr (tGFP-tagged) - Mouse breakpoint cluster region (Bcr), (10ug) |
CNY 12350.00 |
|
| MR222863 | Bcr (Myc-DDK-tagged) - Mouse breakpoint cluster region (Bcr) |
CNY 8840.00 |
|
| MR222863L3 | Lenti ORF clone of Bcr (Myc-DDK-tagged) - Mouse breakpoint cluster region (Bcr) |
CNY 13110.00 |
|
| MR222863L4 | Lenti ORF clone of Bcr (mGFP-tagged) - Mouse breakpoint cluster region (Bcr) |
CNY 13110.00 |
