Sin3a (NM_011378) Mouse Untagged Clone
CAT＃: MC224127
Sin3a (untagged) - Mouse transcriptional regulator, SIN3A (yeast) (Sin3a), transcript variant 1, (10ug)
CNY 13312.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW553200; mKIAA4126; mSin3A; Sin3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224127 representing NM_011378
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGACGTTTGGATGACCAGGAATCACCAGTGTATGCAGCCCAGCAGCGAAGGATTCCTGGGAGCA CAGAGGCTTTTTCTCACCAGCACCGGGTCCTTGCCCCGGCCCCTCCTGTGTATGAAGCAGTGTCTGAGAC CATGCAGTCAGCTACAGGCATTCAGTACTCAGTGGCACCCAACTACCAGGTTTCAGCTGTGCCACAAAGT TCTGGCAGTCATGGGCCCGCCATAGCAGCAGTTCATAGCAGCCATCATCACCCAACAGCTGTCCAGCCTC ATGGAGGCCAGGTGGTCCAGAGCCATGCCCACCCAGCACCACCAGTTGCACCAGTACAGGGACAGCAGCA GTTTCAGAGGCTCAAGGTGGAAGACGCCCTGTCCTATCTTGACCAGGTGAAACTGCAGTTCGGTAGTCAG CCTCAGGTCTACAATGATTTCCTTGACATCATGAAGGAATTTAAATCTCAGAGCATTGATACTCCAGGAG TGATTAGCCGAGTGTCCCAGCTATTTAAAGGCCACCCTGATCTGATCATGGGCTTTAACACCTTCTTGCC TCCTGGCTACAAAATTGAGGTGCAGACTAATGACATGGTGAACGTGACAACACCTGGCCAAGTTCATCAG ATTCCCACCCATGGCATCCAGCCCCAGCCTCAGCCACCACCTCAGCATCCTTCCCAGCCTTCATCCCAGT CAGCTCCCACTCCTGCTCAGCCAGCTCCTCAGCCCACAGCTGCCAAAGTCAGCAAGCCTTCCCAACTACA AGCACATACTCCAGCCAGTCAGCAGACTCCCCCACTCCCACCATATGCATCCCCACGTTCTCCACCAGTC CAGCCTCACACACCAGTGACAATCTCCTTGGGGACAGCTCCATCTTTGCAAAACAATCAGCCTGTGGAGT TTAATCATGCCATCAACTATGTTAATAAGATCAAGAACAGATTCCAGGGCCAACCAGACATCTACAAAGC ATTCTTGGAGATTTTGCACACATACCAGAAAGAACAGCGGAATGCCAAGGAAGCTGGAGGAAACTACACT CCAGCTTTGACTGAGCAAGAGGTGTATGCCCAGGTGGCTCGACTCTTCAAAAACCAGGAAGATTTGTTGT CTGAATTTGGACAGTTCCTGCCAGATGCCAACAGCTCAGTGCTTTTAAGCAAAACAACTGCTGAGAAGGT TGATTCTGTGAGAAATGACCATGGAGGCACTGTGAAGAAGCCCCAACTGAATAACAAGCCACAGAGGCCC AGTCAGAATGGCTGCCAGATCCGCAGGCACTCTGGAACAGGAGCCACACCTCCAGTGAAGAAAAAACCCA AACTGATGAGTCTAAAAGAGTCTTCAATGGCAGATGCCAGCAAGCATGGTGTTGGAACGGAATCATTATT TTTTGATAAGGTTCGAAAGGCTCTTCGGAGTGCAGAGGCCTATGAAAACTTCCTTCGTTGCCTTGTTATC TTTAATCAGGAGGTGATCTCTCGGGCCGAGCTTGTACAGCTAGTCTCTCCTTTTCTGGGGAAATTCCCTG AATTGTTTAATTGGTTTAAAAACTTTTTGGGCTATAAGGAGTCTGTACATCTGGAAAGCTTTCCAAAGGA ACGAGCTACAGAAGGCATTGCCATGGAGATAGACTATGCCTCTTGTAAACGACTGGGCTCTAGCTACCGA GCCCTACCGAAAAGTTACCAGCAGCCCAAGTGCACGGGACGGACTCCTCTGTGTAAAGAGGTTTTAAATG ATACCTGGGTTTCCTTCCCATCTTGGTCTGAAGACTCCACTTTTGTTAGTTCCAAGAAGACTCAGTATGA AGAACATATTTACCGTTGTGAAGATGAACGATTTGAGCTTGATGTGGTTCTTGAGACCAATCTTGCAACA ATCCGGGTTTTAGAAGCAATACAGAAAAAACTTTCTCGCTTGTCTGCTGAGGAACAAGCCAAATTTCGCT TGGATAACACCCTTGGAGGCACGTCCGAAGTCATCCATCGAAAAGCACTCCAGAGGATATATGCTGACAA AGCAGCTGATATCATCGATGGCCTGAGGAAGAACCCCTCCATTGCTGTTCCGATTGTCCTTAAAAGGTTG AAGATGAAAGAAGAAGAGTGGCGAGAAGCTCAGAGAGGCTTCAACAAGGTCTGGCGAGAGCAAAATGAGA AGTACTACTTGAAGTCTCTGGATCACCAAGGCATCAACTTCAAGCAGAACGACACTAAGGTCTTGAGGTC TAAGAGCTTACTCAATGAGATCGAGAGCATCTATGACGAGAGGCAAGAGCAGGCTACAGAAGAGAACGCT GGTGTACCTGTTGGCCCGCACCTCTCTCTTGCCTATGAAGACAAACAGATACTAGAAGATGCTGCTGCTC TGATTATCCACCATGTGAAGAGGCAAACAGGCATTCAGAAAGAGGACAAATACAAAATCAAGCAAATCAT GCACCATTTCATTCCTGACCTGCTGTTTGCTCAGAGAGGCGATCTCTCAGATGTGGAAGAAGAGGAGGAG GAAGAAATGGATGTGGATGAAGCAACAGGAGCACCTAAGAAGCACAATGGTGTTGGGGGCAGCCCCCCTA AGTCCAAGTTGCTATTTAGTAACACAGCAGCTCAAAAGTTAAGAGGGATGGATGAAGTATATAACCTTTT CTATGTCAATAACAATTGGTATATCTTTATGCGACTGCATCAAATTCTCTGCTTGAGGCTGCTACGGATT TGTTCCCAAGCTGAACGGCAAATTGAAGAAGAAAACCGAGAGAGAGAATGGGAACGGGAGGTGCTAGGCA TAAAGCGAGACAAGAGTGATAGTCCTGCCATACAACTACGTCTCAAGGAACCTATGGATGTTGATGTAGA AGATTATTACCCAGCTTTCCTGGACATGGTGCGGAGCCTGCTTGATGGCAACATAGACTCATCACAGTAT GAAGATTCACTGAGAGAGATGTTCACCATTCATGCCTACATTGCCTTTACTATGGACAAATTAATCCAGA GCATCGTCAGACAGCTACAGCACATCGTCAGCGACGAGGTCTGTGTGCAGGTTACTGATCTTTACTTGGC AGAAAACAATAACGGAGCCACGGGAGGCCAGCTCAACAGTCAGACTTCAAGGAGCCTTCTGGAGTCAGCA TACCAGCGGAAGGCAGAGCAGCTTATGTCAGATGAGAACTGCTTCAAGCTAATGTTCATTCAAAGTCAAG GTCAAGTTCAGCTGACTGTTGAGCTCCTGGACACAGAAGAGGAGAACTCAGATGACCCCGTGGAAGCAGA GCGTTGGTCAGACTACGTGGAGCGATATATGAGTTCTGATACTACTTCTCCTGAACTTCGAGAACATCTG GCACAGAAACCAGTATTTCTCCCAAGGAATTTGCGGCGTATCCGGAAGTGTCAACGTGGTCGAGAGCAAC AGGAAAAAGAAGGGAAAGAAGGAAACAGCAAGAAGACCATGGAAAATGTAGAGAGCCTGGATAAGCTGGA GTGTAGGTTCAAGCTGAACTCCTATAAGATGGTATATGTGATCAAATCGGAGGACTACATGTACCGGAGA ACTGCTCTACTCAGAGCTCATCAGTCCCATGAGCGTGTAAGCAAGCGTCTGCATCAGCGGTTCCAGGCCT GGGTGGATAAATGGACCAAGGAGCATGTGCCTCGGGAAATGGCAGCAGAGACCAGCAAATGGCTCATGGG TGAGGGGCTCGAGGGCCTGGTACCCTGCACCACCACCTGTGATACAGAGACTCTGCACTTTGTGAGCATT AACAAATATCGTGTCAAATACGGCACAGTATTCAAAGCCCCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011378 |
| Insert Size | 3825 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011378.2, NP_035508.2 |
| RefSeq Size | 5229 bp |
| RefSeq ORF | 3825 bp |
| Locus ID | 20466 |
| UniProt ID | Q60520 |
| Gene Summary | Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (PubMed:22476904). Required for cortical neuron differentiation and callosal axon elongation (PubMed:27399968).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript but encodes the shorter isoform (1). Variants 1, 3, and 4 all encode the same isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223187 | Sin3a (tGFP-tagged) - Mouse transcriptional regulator SIN3A (yeast) (Sin3a) transcript variant 1, (10ug) |
CNY 13970.00 |
|
| MR223187 | Sin3a (Myc-DDK-tagged) - Mouse transcriptional regulator, SIN3A (yeast) (Sin3a), transcript variant 1 |
CNY 12730.00 |
|
| MR223187L3 | Lenti ORF clone of Sin3a (Myc-DDK-tagged) - Mouse transcriptional regulator, SIN3A (yeast) (Sin3a), transcript variant 1 |
CNY 14630.00 |
|
| MR223187L4 | Lenti ORF clone of Sin3a (mGFP-tagged) - Mouse transcriptional regulator, SIN3A (yeast) (Sin3a), transcript variant 1 |
CNY 14630.00 |
