Cand1 (NM_027994) Mouse Untagged Clone
CAT＃: MC224016
Cand1 (untagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1), (10ug)
CNY 15770.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2310038O07Rik; 6330512O03Rik; AI195005; AI846556; D10Ertd516e; mKIAA0829; Tp120a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224016 representing NM_027994
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCGCTTCGTACCACATCTCCAACTTGCTGGAGAAAATGACATCCAGCGACAAGGACTTCAGGT TCATGGCGACCAACGACCTGATGACAGAGCTGCAGAAAGACTCTATCAAGCTGGACGATGACAGCGAGAG GAAAGTTGTGAAGATGATTCTCAGGTTGCTGGAGGATAAAAATGGCGAAGTGCAGAACTTAGCTGTCAAA TGCCTTGGCCCTTTGGTGAGTAAAGTGAAAGAGTACCAAGTGGAGACGATCGTGGACACCCTCTGCACCA ACATGCTTTCTGACAAGGAGCAGCTGCGAGACATTTCCAGCATCGGCCTGAAAACAGTAATTGGAGAACT TCCGCCAGCTTCCAGTGGCTCTGCATTAGCTGCTAATGTATGTAAAAAGATTACTGGACGCCTTACCAGT GCAATAGCAAAGCAGGAAGATGTTTCTGTTCAGCTCGAAGCCTTGGATATCATGGCTGACATGTTGAGCA GGCAAGGAGGACTGCTTGTTAATTTCCACCCTTCAATTCTGACCTGTCTGCTTCCCCAGCTGACCAGCCC TAGACTTGCAGTGAGGAAAAGAACAATTATTGCTCTTGGCCACCTAGTTATGAGCTGCGGAAATATAGTT TTTGTAGACCTTATTGAACATCTGTTGTCAGAGTTGTCCAAAAATGACTCCATGTCAACAACAAGGACCT ACATACAGTGTATTGCTGCTATTAGTAGGCAAGCTGGCCATAGAATAGGTGAATACCTTGAAAAGATAAT TCCTTTGGTGGTAAAATTTTGTAATGTAGATGATGATGAATTAAGAGAATACTGCATTCAAGCTTTTGAA TCATTTGTGAGAAGATGCCCTAAAGAAGTTTATCCTCATGTTTCTACCATTATAAACATTTGTCTAAAAT ACCTTACCTATGATCCAAATTACAATTACGATGATGAAGATGAAGATGAGAATGCTATGGATGCTGACGG TGGGGACGACGATGACCAAGGGAGTGACGATGAATATAGTGACGATGACGACATGAGTTGGAAAGTGAGA CGCGCGGCTGCTAAGTGCCTGGACGCTGTAGTTAGCACACGGCATGAGATGCTCCCGGAGTTCTACAAGA CTGTCTCTCCTGCGCTGATAGCCAGATTTAAAGAGCGGGAAGAAAATGTAAAGGCAGATGTTTTTCATGC ATACCTGTCTCTTCTGAAACAAACTCGTCCAGTGCAAAGTTGGCTGTGTGACCCTGACGCAATGGAGCAG GGAGACACGCCCCTAACAATGCTGCAGAGTCAGGTTCCCAACATTGTTAAAGCCCTGCACAAGCAGATGA AAGAGAAGAGTGTGAAGACCCGACAGTGTTGCTTTAACATGTTAACTGAGCTGGTAAATGTGCTGCCGGG AGCACTAACCCAGCACATTCCTGTACTGGTACCAGGAATCATTTTCTCACTGAATGATAAGTCCAGCTCA TCAAACCTGAAGATTGATGCGCTGTCGTGTCTGTATGTCATCCTCTGTAACCACTCTCCTCAAGTTTTCC ATCCTCATGTTCAGGCTCTGGTCCCTCCGGTGGTGGCCTGTGTGGGAGACCCGTTTTACAAGATCACTTC AGAAGCCCTTCTTGTCACTCAGCAGCTTGTCAAAGTGATCCGTCCTCTAGACCAGCCCTCTTCGTTCGAT GCAACGCCTTACATCAAAGATCTCTTCACCTGCACAATTAAGCGCTTAAAAGCAGCTGACATTGATCAAG AAGTCAAGGAAAGGGCTATTTCCTGTATGGGACAGATTATTTGCAATCTTGGAGACAATTTGGGTCCTGA TTTATCAAATACCCTACAGATTTTCTTGGAGAGACTCAAGAATGAGATTACCCGGCTAACGACCGTCAAA GCGCTGACCCTGATTGCTGGGTCACCTTTGAAGATAGATCTGAGGCCTGTGCTGGGAGAGGGAGTCCCCA TCCTTGCTTCATTCCTCAGGAAAAATCAGAGAGCTTTGAAACTGGGGACCCTCTCTGCCCTGGATATTCT CATTAAGAACTACAGTGACAGCCTGACGGCCGCCATGATTGACGCAGTTCTGGATGAGCTCCCCCCCCTC ATCAGCGAGAGCGATATGCACGTGTCGCAGATGGCTATCAGCTTCCTCACCACCCTGGCCAAGGTCTATC CCTCCTCCCTGTCCAAGATAAGCGGATCTATCCTCAATGAACTGATTGGACTTGTGAGATCGCCTCTACT GCAGGGAGGAGCGCTCAGTGCCATGCTAGACTTTTTCCAAGCTCTGGTTGTCACTGGAACAAACAATCTA GGGTACATGGACTTGCTGCGCATGCTAACGGGTCCAGTTTATTCTCAGAGCACTGCTCTCACTCATAAGC AGTCTTATTATTCCATTGCCAAATGTGTAGCTGCCCTTACTCGAGCTTGCCCTAAAGAGGGACCCGCCGT AGTAGGTCAGTTTATTCAAGATGTCAAGAACTCAAGGTCTACAGATTCCATTCGTCTCTTAGCACTCCTT TCTCTTGGAGAAGTTGGACACCATATTGACTTAAGTGGGCAGTTGGAATTAAAATCTGTAATATTAGAGG CTTTCTCATCCCCTAGTGAAGAAGTCAAATCAGCTGCGTCCTATGCATTAGGCAGCATTAGTGTAGGCAA CCTTCCTGAGTATCTGCCCTTTGTACTGCAAGAAATAACCAGCCAGCCCAAAAGGCAGTATCTTCTGCTT CACTCTTTGAAGGAGATCATTAGCTCTGCATCGGTGGCAGGCCTTAAGCCATATGTTGAGAACATCTGGG CCTTGCTGCTAAAGCACTGTGAGTGTGCGGAGGAAGGCACCAGGAATGTCGTGGCTGAATGTCTAGGGAA GCTCACTCTTATTGATCCTGAAACTCTCCTCCCACGGCTTAAAGGGTATTTGATATCAGGGTCATCATAT GCCAGGAGCTCGGTGGTTACAGCTGTGAAGTTCACGATTTCTGATCATCCTCAGCCTATTGATCCACTGT TGAAGAACTGCATAGGTGACTTTCTAAAAACTTTGGAAGACCCAGATTTGAACGTAAGAAGAGTGGCCTT GGTCACATTCAATTCTGCGGCCCATAACAAGCCATCACTGATACGGGACCTTCTGGACTCAGTTCTTCCA CATCTTTACAATGAGACAAAAGTTAGGAAGGAACTTATAAGAGAGGTAGAAATGGGTCCATTTAAACACA CGGTTGATGACGGTCTAGACATTAGAAAGGCAGCTTTTGAGTGTATGTATACACTTCTAGACAGCTGTCT TGATAGACTGGATATCTTTGAATTTCTAAATCATGTTGAAGATGGTTTGAAGGACCATTATGATATTAAG ATGCTAACATTTTTAATGTTGGTACGACTGTCTACCCTCTGTCCAAGTGCAGTACTACAAAGGCTGGACC GACTTGTTGAGCCACTGCGTGCTACGTGTACAACTAAGGTAAAGGCAAACTCTGTAAAGCAGGAGTTTGA AAAGCAAGATGAGCTAAAGCGGTCTGCCATGAGGGCAGTGGCGGCTTTGTTAACCATTCCAGAGGCAGAG AAGAGCCCTCTTATGAGTGAATTCCAGTCACAGATCAGCTCCAACCCGGAGCTGGCAGCCATCTTTGAAA GTATCCAGAAAGATTCTTCGTCCACCAACTTGGAATCAATGGACACGAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_027994 |
| Insert Size | 3693 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_027994.1, NP_082270.1 |
| RefSeq Size | 8209 bp |
| RefSeq ORF | 3693 bp |
| Locus ID | 71902 |
| UniProt ID | Q6ZQ38 |
| Gene Summary | Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG216867 | Cand1 (tGFP-tagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1), (10ug) |
CNY 13490.00 |
|
| MR216867 | Cand1 (Myc-DDK-tagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1) |
CNY 12260.00 |
|
| MR216867L3 | Lenti ORF clone of Cand1 (Myc-DDK-tagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1) |
CNY 14160.00 |
|
| MR216867L4 | Lenti ORF clone of Cand1 (mGFP-tagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1) |
CNY 14160.00 |
