Ankfy1 (NM_009671) Mouse Untagged Clone
CAT＃: MC223788
Ankfy1 (untagged) - Mouse ankyrin repeat and FYVE domain containing 1 (Ankfy1), (10ug)
CNY 15010.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Ankhzn; mKIAA1255; ZFYVE14 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223788 representing NM_009671
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAGAGGAGGTGGCCAAGTTGGAGAAGCATTTGATGCTTCTAAGACAAGAGTATGTCAAGCTGC AGAAGAAACTGGCAGAAACAGAGAAAAGATGCACTCTTTTGGCTGCACAAGCAAACAAGGAGAATAGCAA CGAGTCATTCATCAGCCGCTTGCTGGCCATAGTGGCAGGTCTCTATGAGCAAGAGCAGTACAGTGATTTG AAGATCAAGGTTGGGGACAGGCACATCAGTGCTCACAAGTTTGTCTTGGCCGCCCGCAGTGATAGCTGGA GTCTGGCCAATTTGTCTTCCACAGAGGAGATAGACTTGTCAGATGCGAATCCTGAGGTGACAATGACAAT GCTTCGCTGGATCTATACGGATGAGTTAGAGTTCAGAGAGGATGATGTGTTCCTGACTGAATTGATGAAA CTGGCCAATCGCTTTCAGCTCCAGCTCCTTAGGGAGAGATGTGAGAAGGGTGTTATGTCTCTGGTGAATG TTAGGAACTGTATTCGCTTCTATCAAACAGCAGAGGAATTAAATGCCAGTACGCTGATGAACTATTGTGC AGAAATTATTGCAAGTCACTGGGATGACTTGCGAAAGGAGGACTTCAGTAGCCTGAGTGCTCAGTTGTTA TACAAAATGATCAAATCCAAGACAGAGTACCCATTACATAAAGCTATCAAAGTGGAGAGAGAAGATGTGG TCTTCCTCTATCTGATTGAAATGGATTCCCAGCTACCTGGGAAGCTTAACGAAACAGATCATAATGGAGA TCTTGCGTTAGATCTGGCTCTTTCTCGGCGACTAGAGAGTATTGCCACCACTCTGGTTAGTCATAAAGCT GATGTGGATATGGTAGACAAAAATGGCTGGAGCTTATTACATAAAGGGATCCAAAGAGGAGATCTTTTTG CTTCTACATTCCTCATTAAGAACGGGGCCCTTGTCAATGCTGCCACAGCAGGAGCCCAGGAGACCCCATT ACACCTTGTAGCATTGTACAGTCCAAAGAAATACTCAGCAGATGTGATGTCTGAGATGGCACAGATTGCG GAGGCCCTCCTGCAGGCTGGTGCCAACCCCAACATGCAGGATAGCAAGGGCAGGACTCCTTTACACTTGT CCATCATGGCCAGGAACGACTGTGTGTTCAGTCAGCTGCTGCAGTGTAAACAATTAGATTTAGAGCTGAA AGATCACGAAGGCAGCACTGCACTCTGGCTGGCCGTCCAGTACATCACTGTGTCTTCAGACCAGTCTGTA AACCCCTTTGAAGACCTCCCTGTGGTGAATGGGACATCATTTGATGAGAATAGCTTTGCAGCCAGACTCA TTCAACGCGGCAGCAACACTGATGCTCCTGATGTGATGACAGGAAATTGTTTACTACAAAGGGCAGCTGG GGCTGGAAATGAGGCTGCTGCTCTCTTCCTGGCAACTAGTGGTGCCCATGCCAACCACAGGAACAAATGG GGAGAAACTCCTTTGCATACAGCTTGTCGGCATGGCCTGGCCAACCTCACAGCAGAGCTCCTGCAGCAAG GTGCCAATCCAAACTTACAGACAGAGGAGGCTCTGCCGGTACCTAAAGAGTCTCCCGTCCTAATGAGTTC TGCAGACAGCATCTACCTACAAACACCACTGCACATGGCAATTGCCTATAACCATCCAGATGTGGTTTCT GTCATCTTAGAGCAGAAAGCCAATGCTCTTCATGCCACCAACAACCTACAAATTATTCCAGACTTCAGCC TCAAGGATTCCAGAGACCAAACTGTTCTCGGCCTGGCATTATGGACTGGCATGCACACAATCGCTGCCCA GCTGCTGGGCTCCGGAGCCTCTATCAATGACACCATGTCAGATGGACAGACATTGCTGCACATGGCCATA CAGCGGCAGGACAGCAAGAGCGCTCTCTTCCTACTGGAGCACCAAGCTGATATAAATGTCAGGACTCAGG ACGGGGAGACTGCCCTCCAGCTGGCCATCAAACATCAGCTCCCACTAGTGGTTGATGCCATATGTACTCG AGGAGCTGACATGTCTGTGCCAGATGAAAAAGGGAACCCACCACTGTGGCTTGCCTTGGCAAGTAATCTG GAGGACATTGCTTCCACTCTGGTCAGACATGGTTGCGATGCTACATGCTGGGGCCCAGGACCCAGTGGGT GTCTTCAGACACTTCTGCATAGAGCCGTTGATGAAAACAATGAGTCTACTGCCTGCTTTCTTATTCGCAG TGGCTGTGACGTGAATAGTCCCAGACAACCAGGTACTAATGGAGAAGGAGAAGAGGAAGCAAGGGATGGA CAGACTCCCCTGCATTTGGCAGCCTCATGGGGACTAGAAGAGACAGTACAGTGTCTTTTGGAGTTTGGTG CCAACGTCAATGCACAGGATGCAGAAGGGAGAACGCCTGTCCATGTGGCCATCAGCAACCAGCATAGCGT CATCATTCAGCTGTTGATCTCTCACCCCAACATTGAGTTGAGCGTCCGGGACAGACAAGGGCTGACTCCT TTTGCCTGTGCCATGACTTACAAGAACAATAAGGCAGCTGAAGCCATTTTAAAACGAGAGTCTGGGGCTG CTGAGCAGGTAGATAATAAAGGTCGGAATTTTCTCCATGTGGCAGTTCAGAACTCTGACATTGAAAGTGT CTTGTTCCTGATCAGTGTCCAGGCCAATGTGAACTCCAGAGTCCAGGATGCTTCCAAGTTGACCCCTCTG CACCTTGCTGTTCAGGCCGGCTCAGAGATCATTGTCCGAAATCTGCTTCTTGCAGGAGCCAAGGTGAATG AGTTAACCAAGCATCGTCAGACCGCTCTCCATCTCGCTGCACAGCAGGACCTGCCCACCATCTGCTCAGT CCTCCTAGAGAATGGAGTGGACTTCGCTGCTGTGGATGAGAATGGAAATAATGCTCTTCATCTTGCGGTC ATGCATGGTCGACTCAACAATATCCGGGCTCTTCTGACAGAGTGTACAGTAGATGCTGAAGCCTTTAATC TGAGAGGCCAGTCACCACTACACATTTTGGGACAGTATGGCAAAGAAAATGCAGCAGCCATCTTTGATCT CTTCTTAGAGTGTATGCCTGAGTATCCTCTGGACAAACCAGATGCAGAAGGCAACACAGTGCTGCTCTTA GCATACATGAAAGGGAATGCCAACTTGTGCCGTGCCATCGTTCGGTCTGGGGTTCGCCTTGGGGTGAATA ATAACCAAGGAGTCAACATTTTCAACTACCAGGTTGCCACTAAGCAGCTTCTGTTTAGATTATTAGATAT GCTCTCCAAAGAGCCTCCATGGTGTGACGGCTCCAACTGCTATGAATGTACTGCCAAGTTTGGAGTCACA ACTCGAAAACATCACTGTCGTCACTGCGGCCGTCTTCTTTGCCATAAATGCTCAACCAAGGAGATCCCTA TTATAAAGTTTGACCTGAACAAGCCTGTGCGGGTTTGCAACATTTGCTTTGATGTACTGACTCTGGGTGG AGTCTCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009671 |
| Insert Size | 3510 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009671.5, NP_033801.4 |
| RefSeq Size | 8034 bp |
| RefSeq ORF | 3510 bp |
| Locus ID | 11736 |
| UniProt ID | Q810B6 |
| Gene Summary | Proposed effector of Rab5. Binds to phosphatidylinositol 3-phosphate (PI(3)P). Involved in homotypic early endosome fusion and to a lesser extent in heterotypic fusion of chlathrin-coated vesicles with early endosomes. Required for correct endosomal localization. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Regulates the subcellular localization of the retromer complex in a EHD1-dependent manner. Involved in endosome-to-Golgi transport and biosynthetic transport to late endosomes and lysosomes indicative for a regulation of retromer complex-mediated retrograde transport (By similarity). Involved in macropinocytosis; the function is dependent on Rab5-GTP.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224779 | Ankfy1 (tGFP-tagged) - Mouse ankyrin repeat and FYVE domain containing 1 (Ankfy1), (10ug) |
CNY 11310.00 |
|
| MR224779 | Ankfy1 (Myc-DDK-tagged) - Mouse ankyrin repeat and FYVE domain containing 1 (Ankfy1) |
CNY 8144.00 |
|
| MR224779L3 | Lenti ORF clone of Ankfy1 (Myc-DDK-tagged) - Mouse ankyrin repeat and FYVE domain containing 1 (Ankfy1) |
CNY 12160.00 |
|
| MR224779L4 | Lenti ORF clone of Ankfy1 (mGFP-tagged) - Mouse ankyrin repeat and FYVE domain containing 1 (Ankfy1) |
CNY 12160.00 |
