Cep135 (NM_199032) Mouse Untagged Clone
CAT＃: MC223709
Cep135 (untagged) - Mouse centrosomal protein 135 (Cep135), (10ug)
CNY 14730.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BC062951; Cep4; Gm1044 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223709 representing NM_199032
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTACAGCTGCAGAGAGAAAGTATATTAACATTCGGAAGAGGTTAGACCAGCTGGGCTACCGCCAGA CCCTGTCAGTGGACAGTCTGCCTTTGGTAGAAAAACTTTTCAGTGACCTCGTTCATACGACAGAAAGCCT GCGGCAGTGCAGGTTGTCCTCGGGAAAGGCGGAAAAGGAAAGTGCCAATCTCGATTTTGTTTTGGAACCT TATAAACTTGAAAATACAAGACTGAATAAGGAGAATAATGAATTGTATCTGGAGTTAATGAAGCTCAGAG AATGCTCAGACAAGCACATTAAAGACTTGAAGACGACACTGAAGAAATGTTCCCGTGAGACAGCTGATCT GAAGTTTCTGAACAACCAGTATGTTCACAAGGTCAAAGTCCTGGAGAAAGAGAGCAAAGCCAAGGATGAA AAGATCCAGCAGCTTCAAGAGAAGAACCTGCGTGCTGTGGTGCAGACCCCTGGTGGCAGGAAAAGAAACA TTGCATTTAGGCGCCAGCGGATGCAAATCGATGAACCAGCCCCACCCTCAGAAGTCAGCGCATACCCGGT TCCTCAACCAGAAGACCCATACATTGCGGACCTCCTGCAAGTGGCTGACAACAGGATTCAGGAGCTGCAG GAGGAGGTCCAGCAGCTGCAGGAGAAGCTAGCACAGATGGAGAAAGGAGTGCTGGACTACAGCAAGCAGA TTGAACTGAGAGAACGAGAAATACAGCGACTGTCACTTGCGTTGGATGGCGGTTGTTCCCCTGATGTCCT GTCTCTGGAGACCAGAAATAAAACCAATGAGAAACTCATTGCTCACTTAAATGTCCAGGTTGACTTCCTC CAGCAAGCTAATAAAGAGCTGGAGAAGCATATTCAAGAGCTCATGGAAACCAAGGAAACAGTGACAACTG AAGTTGTGAACCTCAGCAACAGAAACGAGAAGCTCTGCCAGGAGCTAACCGAGATCGACCAGTTAGCCCA GCGGCTGGAAAGGCACAAGGAGCAAGTGCTGGAGACAGCAGACAAGGAGCTCGGGGAGGCAAAGAAAGAG ATTAAACGAAACCTCTGTGAAATGCGGAACCTTGAGGAAAAAATGTCAAAACTGCAGTGGGAATTGGACT TAAGCCATAAGGAGAAGGAGAGACTGAACAGTGAGCTGCTTTTAAAGTCAGACCTGGAGACTGTTGTTCA TCAGCTCGAACAAGAAAAGCAAAGACTTAGCAAAAAGCTGCAGAGTTTTGCAGTCACAGAAAGAGAACTG ACTCTGGAGGTTGAGAGGATGAGGCTAGAACATGGGATAAAGCGTCGGGACAAGTCACCCTCTCGTCTGG ACACATTTCTGAAAGGCATAGAGGAAGAGCGCGATTATTACAAGAAAGAGCTGGAGAAACTGCAGCATCT AATCCAGCGGAGATCTTGTGCGATTAATTACTCCGCTCGGGAAAAACCTCCTGTAGTCAAATGCTCAGAA AAGGGTGACTGCAGCACGGACGTTCACCTGATCACAAGAGAAAGGGATGAACTTCAGCGCATGCTGGAGA GATTCGAGAAATACATGGAGGACATACAGTCCAACGTGAAGCTGCTGACGGCGGAAAGGGACAAGCTGAA TGTCCTGTATAAGGAAGCAAAGGAAGAGTTGTCTACACTAAGGAAGGAGTCCACTAATTCGACATCCCCC AACCATCTTGTTAGTTGTGTAGAAAAGGAGAAAGAACGCGCGTTATCTGAGTTGAGAAGAATTACAGCAG AAAAGGAAGCTCTGAGAGAAAAGTTGAAAAACATCCAAGAAAGGAATGCTGTTGGAAAATCAGACTTAGA GAAAACTATTGAACATTTGACATATATTAATCACCAGCTTGAAAATGAAAAATATGAATTGCAATCTAAA ATGTTAATGATGAAAGAAACAGTGGAGTCATTAGAGAACAAATCAAAACTCCAAGCTCAAAAGCTTAGCC ATGTGACTGGTGACTCATCTCATCAGAAAACAGAGATGACCTCCCTTAGGATCGTCAGTGAGCAGCTACA GCGCTCACTTGATGACTGTCAGCACCGACTTTCCATAAAAAGAGGTGAACTTGAATCAGCCCAAGAGCAA ATTAAAATGCTGGAGCAAAAACTAGAGAATCTCAGCCACAGGATGACGGTGCAGAGCGAAGAGACTCACG CCATGAAGAAGACCATTGGAGTGATGGACAAAGAAAAGGACTTTCTCCAGGAGACTGTGGATGAGAAGAC AGAAAAGATCGCCAACCTGCAGGAAAGCCTCCTTAGTAAAGAAAAAGTCATTGCCCAGTTGAAGGTCACA GTTGCAGAGTATGAAACATCACTTAACCAGCTACAAGAAACTTTGACTACTCGAGACCGAGAGATAAACA GCCTCCGGCGCCAGCTTGATGCATCTCACAAGGAGCTTGATGATGTTGGGAAATCTAGAGAGATCTCTTT TAAGGAAAATAGAAGGTTACAAGATGACCTGGCCACAATGGCGAGAGAAAATCAGGAGATCTCACTGGAG CTGGAAGCAGCAGTGCAAGAAAAAGAAGAGATGAAGAGCAGAGTCCATAAGTACATCACTGAGGTGTCGC GGTGGGAGAGCCTGATGGCCGCCAAGGAAAAAGAAAACAAAGACTTGCTGGATAGATTCCAGATGCTCCA CAGCCGTGCTGAGGACTGGGAGGTCAAGGCTCAGCAAGCGGAAGGGGAGAACAGCTCAGTCCGCCTGGAG CTGCTGTCTATCGACACAGAGAGAAGGCATCTGCGGGAACGAGTGGACCTCCTGGAGAAGGAGATCCAGG AGCACATCAATGCACATCACGCATACGAGTCTCAGATCTCGTCAATGGCTAAGGCCATGTCTCAGTTAGA AGAGGAGCTGCGACGTCATGAGAGCGAGAAAGCCACCATGTTAGGTGACGTGTCGTCTCTCCGAGAACTG TGCATCAAGCTCGACTCAGGCAAAGACGTCATGACCCAGCAGCTGAATTCCAAAAGCCTAGAGCTGGAGC GGGCGGTAGCAGAATTAGAGAATGTAAAATCAGAATCAGAACTATTAAAAAAACAGCTGACGAATGAGAG ACAGACAATTAAAAACCTTGAGTCGTTGTTAGCTACAAACAGAGATAAAGAGTTCCAGTCCCATTTAACC TCCCACGAGAAGGACACAGAAATCCAGCTGCTGAAGGAGAAGCTAAATCTCTCAGAAAGCAAACTGACTA CCCAAAGCCGAGAAACCTCCATGCTTCGAACTAAAGTGACACAGTTGCAGACAGATTATGATAATCTGAA GAGGCAGATGTCAAACGAGAAGTACGAACGAGAGCGAGCAATCCAAGAGATGCGCCGACTTGGTCTTCCT ACATCACCTCTGAGCTCTACTCTGAAATCTCCTGTACAGACTCCTGATCATATAAATGCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_199032 |
| Insert Size | 3423 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_199032.2, NP_950197.1 |
| RefSeq Size | 5334 bp |
| RefSeq ORF | 3423 bp |
| Locus ID | 381644 |
| UniProt ID | Q6P5D4 |
| Gene Summary | Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211699 | Cep135 (tGFP-tagged) - Mouse centrosomal protein 135 (Cep135) |
CNY 9220.00 |
|
| MR211699 | Cep135 (Myc-DDK-tagged) - Mouse centrosomal protein 135 (Cep135) |
CNY 7936.00 |
|
| MR211699L3 | Lenti ORF clone of Cep135 (Myc-DDK-tagged) - Mouse centrosomal protein 135 (Cep135) |
CNY 10260.00 |
|
| MR211699L4 | Lenti ORF clone of Cep135 (mGFP-tagged) - Mouse centrosomal protein 135 (Cep135) |
CNY 10260.00 |
